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Protein

Forkhead box protein P4

Gene

Foxp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor that represses lung-specific expression.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri312 – 33726C2H2-typeSequence AnalysisAdd
BLAST
DNA bindingi459 – 54991Fork-headPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB

GO - Biological processi

  • embryonic foregut morphogenesis Source: MGI
  • heart development Source: MGI
  • lung secretory cell differentiation Source: MGI
  • negative regulation of lung goblet cell differentiation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of lung goblet cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein P4
Alternative name(s):
Fork head-related protein-like A
Short name:
mFKHLA
Gene namesi
Name:Foxp4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1921373. Foxp4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi366 – 3661Missing : Loss of dimerization. Almost complete loss of DNA-binding. Reduced transcriptional repression activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 795795Forkhead box protein P4PRO_0000247653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei652 – 6521PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9DBY0.
PRIDEiQ9DBY0.

PTM databases

PhosphoSiteiQ9DBY0.

Expressioni

Tissue specificityi

Expressed in the adult heart, brain, spleen lung, liver, kidney and testes.2 Publications

Developmental stagei

Expressed predominantly in the lung and brain during embryogenesis. Expressed in the lung epithelium and the mesenchyme immediately adjacent to the epithelium from 10.5 dpc. At 9.5 dpc, expressed in the foregut endoderm but not in the heart. At 16.5 and 18.5 dpc, expressed in both the proximal and distal airway epithelium. Also expressed in the developing gut. In the hindgut, primarily expressed in epithelial cells of the intestine and stomach. Expressed in the brain in a dynamic pattern. At 14.5 dpc, expressed at high levels in the intermediate zone of the neopallial cortex with lower levels in the surrounding cells. By 16.5 dpc, no longer expressed in the intermediate zone, but still present in the surrounding cells of the neopallial cortex.2 Publications

Gene expression databases

BgeeiQ9DBY0.
CleanExiMM_FOXP4.
ExpressionAtlasiQ9DBY0. baseline and differential.
GenevisibleiQ9DBY0. MM.

Interactioni

Subunit structurei

Forms homodimers and heterodimers with FOXP1 and FOXP2. Dimerization is required for DNA-binding.1 Publication

Protein-protein interaction databases

BioGridi216509. 2 interactions.
IntActiQ9DBY0. 1 interaction.
STRINGi10090.ENSMUSP00000094916.

Structurei

3D structure databases

ProteinModelPortaliQ9DBY0.
SMRiQ9DBY0. Positions 317-375, 458-539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni354 – 37522Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi71 – 225155Gln-richSequence AnalysisAdd
BLAST
Compositional biasi603 – 724122Pro-richSequence AnalysisAdd
BLAST

Domaini

The leucine-zipper is required for dimerization and transcriptional repression.1 Publication

Sequence similaritiesi

Contains 1 C2H2-type zinc finger.Sequence Analysis
Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri312 – 33726C2H2-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5025.
GeneTreeiENSGT00780000121840.
HOGENOMiHOG000092089.
HOVERGENiHBG051657.
InParanoidiQ9DBY0.
KOiK09409.
OrthoDBiEOG7M6D7G.
PhylomeDBiQ9DBY0.
TreeFamiTF326978.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. TF_fork_head.
IPR011991. WHTH_DNA-bd_dom.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q9DBY0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMVESASETI RSAPSGQNGV GSLSAQADGG GGAGTAGTAP AAGRDASGRE
60 70 80 90 100
AASGGADSNG EMSPAELLHF QQQQALQVAR QFLLQQASSL NSPGNNDSKQ
110 120 130 140 150
SASAVQVPVS VAMMSQQMLT PQQMQQILSP PQLQALLQQQ QALMLQQLQE
160 170 180 190 200
YYKKQQEQLH LQLLTQQQAG KQQPKEALGN KQLAFQQQLL QMQQLQQQHL
210 220 230 240 250
LNLQRQGLVS LQPSQASGPL QALPQAVCPT DLPQLWKGEG APGQPAEDSG
260 270 280 290 300
RQEGLDLAST AVTATSFASP PKVSPPLSHH PLPNGQPTVL TSRRDSSSHE
310 320 330 340 350
ETPSSHPLYG HGECKWPGCE TLCEDLGQFI KHLNTEHALD DRSTAQCRVQ
360 370 380 390 400
MQVVQQLEIQ LAKESERLQA MMAHLHMRPS EPKPFSQPVT VSADPFPDGL
410 420 430 440 450
VHPPTSAAAP VTPLRPPGLG SASLHSGGPA RRRSNDKFCS PISSELAQNH
460 470 480 490 500
EFYKNADVRP PFTYASLIRQ AILETPDRQL TLNEIYNWFT RMFAYFRRNT
510 520 530 540 550
ATWKNAVRHN LSLHKCFVRV ENVKGAVWTV DEREYQKRRP PKMTGSPTLV
560 570 580 590 600
KNMISGLSYG ALNASYQAAL AESSFPLLSN PGMLNPGSAS SLLPLSQEDL
610 620 630 640 650
GVPGEPLPSN GSSSPPRLSP PQYSHQIQVK EEPAEAEEDR RPGPPLGAPN
660 670 680 690 700
PSTVGPPEDR DLEEDLGGED MPSQPCPLIP GWKPSLLHLS YCVKPKFTVS
710 720 730 740 750
VGSKTPSSPL PPPPRVQGSY SLPPCSYLAY GDMRGQNPAP SPGLLSGVGG
760 770 780 790
GLFRCLHRTK SPSLPGVWIL AAELETMRFH RPPMGDPQPK TADWV
Note: No experimental confirmation available.Curated
Length:795
Mass (Da):85,981
Last modified:June 1, 2001 - v1
Checksum:i860AE60AAD3742CE
GO
Isoform 21 Publication (identifier: Q9DBY0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     672-672: P → S
     673-795: Missing.

Show »
Length:672
Mass (Da):72,759
Checksum:i8B89F59EF85FC768
GO
Isoform 31 Publication (identifier: Q9DBY0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     388-388: P → PLNPVPGSSSFSK
     672-672: P → S
     673-795: Missing.

Show »
Length:684
Mass (Da):73,960
Checksum:iA7CFBE31E1C09A09
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti226 – 2261A → AA in AAN08624 (Ref. 2) Curated
Sequence conflicti226 – 2261A → AA in AAH43702 (PubMed:15489334).Curated
Sequence conflicti226 – 2261A → AA in AAH52407 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei388 – 3881P → PLNPVPGSSSFSK in isoform 3. 2 PublicationsVSP_052129
Alternative sequencei672 – 6721P → S in isoform 2 and isoform 3. 3 PublicationsVSP_052130
Alternative sequencei673 – 795123Missing in isoform 2 and isoform 3. 3 PublicationsVSP_052131Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135029 mRNA. Translation: AAN08624.1.
AB052766 mRNA. Translation: BAC53799.1.
AK004693 mRNA. Translation: BAB23479.1.
BC043702 mRNA. Translation: AAH43702.1.
BC052407 mRNA. Translation: AAH52407.1.
BC057110 mRNA. Translation: AAH57110.1.
CCDSiCCDS50135.1. [Q9DBY0-2]
RefSeqiNP_001104294.1. NM_001110824.1.
NP_001104295.1. NM_001110825.1.
NP_083043.2. NM_028767.2. [Q9DBY0-2]
XP_006525079.1. XM_006525016.2.
XP_006525080.1. XM_006525017.2. [Q9DBY0-3]
UniGeneiMm.240062.

Genome annotation databases

EnsembliENSMUST00000113265; ENSMUSP00000108890; ENSMUSG00000023991. [Q9DBY0-2]
GeneIDi74123.
KEGGimmu:74123.
UCSCiuc008cwm.2. mouse. [Q9DBY0-3]
uc008cwn.2. mouse. [Q9DBY0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY135029 mRNA. Translation: AAN08624.1.
AB052766 mRNA. Translation: BAC53799.1.
AK004693 mRNA. Translation: BAB23479.1.
BC043702 mRNA. Translation: AAH43702.1.
BC052407 mRNA. Translation: AAH52407.1.
BC057110 mRNA. Translation: AAH57110.1.
CCDSiCCDS50135.1. [Q9DBY0-2]
RefSeqiNP_001104294.1. NM_001110824.1.
NP_001104295.1. NM_001110825.1.
NP_083043.2. NM_028767.2. [Q9DBY0-2]
XP_006525079.1. XM_006525016.2.
XP_006525080.1. XM_006525017.2. [Q9DBY0-3]
UniGeneiMm.240062.

3D structure databases

ProteinModelPortaliQ9DBY0.
SMRiQ9DBY0. Positions 317-375, 458-539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216509. 2 interactions.
IntActiQ9DBY0. 1 interaction.
STRINGi10090.ENSMUSP00000094916.

PTM databases

PhosphoSiteiQ9DBY0.

Proteomic databases

MaxQBiQ9DBY0.
PRIDEiQ9DBY0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113265; ENSMUSP00000108890; ENSMUSG00000023991. [Q9DBY0-2]
GeneIDi74123.
KEGGimmu:74123.
UCSCiuc008cwm.2. mouse. [Q9DBY0-3]
uc008cwn.2. mouse. [Q9DBY0-2]

Organism-specific databases

CTDi116113.
MGIiMGI:1921373. Foxp4.

Phylogenomic databases

eggNOGiCOG5025.
GeneTreeiENSGT00780000121840.
HOGENOMiHOG000092089.
HOVERGENiHBG051657.
InParanoidiQ9DBY0.
KOiK09409.
OrthoDBiEOG7M6D7G.
PhylomeDBiQ9DBY0.
TreeFamiTF326978.

Miscellaneous databases

ChiTaRSiFoxp4. mouse.
NextBioi339838.
PROiQ9DBY0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DBY0.
CleanExiMM_FOXP4.
ExpressionAtlasiQ9DBY0. baseline and differential.
GenevisibleiQ9DBY0. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. TF_fork_head.
IPR011991. WHTH_DNA-bd_dom.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Foxp4: a novel member of the Foxp subfamily of winged-helix genes co-expressed with Foxp1 and Foxp2 in pulmonary and gut tissues."
    Lu M.M., Li S., Yang H., Morrisey E.E.
    Mech. Dev. 119:S197-S202(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6Imported.
    Tissue: Lung1 Publication.
  2. "Isolation and characterization of novel human and mouse genes, which are expressed in the digestive tract."
    Daigo Y., Takayama I., Fujino M.A.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6JImported.
    Tissue: LungImported.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Strain: C57BL/6Imported and FVB/NImported.
    Tissue: BrainImported and Mammary gland1 Publication.
  5. Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. "Transcriptional and DNA binding activity of the Foxp1/2/4 family is modulated by heterotypic and homotypic protein interactions."
    Li S., Weidenfeld J., Morrisey E.E.
    Mol. Cell. Biol. 24:809-822(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DIMERIZATION, DOMAIN, MUTAGENESIS OF GLU-366.

Entry informationi

Entry nameiFOXP4_MOUSE
AccessioniPrimary (citable) accession number: Q9DBY0
Secondary accession number(s): Q80V92, Q8CG10, Q8CIS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.