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Q9DBX2 (PHLP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosducin-like protein

Short name=PHLP
Gene names
Name:Pdcl
Synonyms:PhLP1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers. Ref.3

Subunit structure

Forms a complex with the beta and gamma subunits of the GTP-binding protein, transducin. Interacts with the CCT chaperonin complex By similarity.

Disruption phenotype

Conditional deletion in photoreceptor cells leads to 50-fold decrease in Gbeta-Ggamma dimer formation and more than 10-fold decrease in light sensitivity. A 20-fold reduction in Gbeta5 and RGS9-1 expression is also observed, causing a 15-fold delay in the shutoff of light responses. Ref.3

Sequence similarities

Belongs to the phosducin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 301300Phosducin-like protein
PRO_0000163756

Regions

Region158 – 301144Thioredoxin fold By similarity

Amino acid modifications

Modified residue21N-acetylthreonine By similarity
Modified residue251Phosphoserine Ref.2
Modified residue2931Phosphoserine By similarity
Modified residue2961Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9DBX2 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: FE0A130521AD4CFC

FASTA30134,407
        10         20         30         40         50         60 
MTTLDDKLLG EKLQYYYSTS EDEDSDHEDK DRGRGAPAIS STPAEAELAG EGISINTGPK 

        70         80         90        100        110        120 
GVINDWRRFK QLETEQREEQ CREMERLIKK LSMSCRSHLD EEEEQQKQKD LQEKISGKMT 

       130        140        150        160        170        180 
LKEFGTKDKN LDDEEFLQQY RKQRMEEMRQ QFHKGPQFKQ VFEIPSGEGF LDMIDKEQKS 

       190        200        210        220        230        240 
TLIMVHIYED GVPGTEAMNG CMICLATEYP AVKFCRVRSS VIGASSRFTR NALPALLIYK 

       250        260        270        280        290        300 
AGELIGNFVR VTDQLGEDFF AVDLEAFLQE FGLLPEKEVL VLTSVRNSAT CHSEDSDLEI 


D 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Lung and Skin.
[2]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[3]"Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors."
Lai C.W., Kolesnikov A.V., Frederick J.M., Blake D.R., Jiang L., Stewart J.S., Chen C.K., Barrow J.R., Baehr W., Kefalov V.J., Willardson B.M.
J. Neurosci. 33:7941-7951(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK004704 mRNA. Translation: BAB23489.1.
AK028668 mRNA. Translation: BAC26056.1.
AK028814 mRNA. Translation: BAC26133.1.
AK166983 mRNA. Translation: BAE39165.1.
CCDSCCDS15998.1.
RefSeqNP_080452.2. NM_026176.3.
XP_006498314.1. XM_006498251.1.
XP_006498315.1. XM_006498252.1.
UniGeneMm.30709.

3D structure databases

ProteinModelPortalQ9DBX2.
SMRQ9DBX2. Positions 51-277.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9DBX2.

Proteomic databases

MaxQBQ9DBX2.
PaxDbQ9DBX2.
PRIDEQ9DBX2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000009174; ENSMUSP00000009174; ENSMUSG00000009030.
ENSMUST00000112940; ENSMUSP00000108562; ENSMUSG00000009030.
GeneID67466.
KEGGmmu:67466.
UCSCuc008jmp.2. mouse.

Organism-specific databases

CTD5082.
MGIMGI:1914716. Pdcl.

Phylogenomic databases

eggNOGNOG262340.
GeneTreeENSGT00530000062970.
HOGENOMHOG000232010.
HOVERGENHBG003456.
InParanoidQ9DBX2.
OMAMQEYNML.
OrthoDBEOG7H4DVC.
PhylomeDBQ9DBX2.
TreeFamTF315179.

Gene expression databases

ArrayExpressQ9DBX2.
BgeeQ9DBX2.
CleanExMM_PDCL.
GenevestigatorQ9DBX2.

Family and domain databases

Gene3D1.10.168.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR001200. Phosducin.
IPR023196. Phosducin_N_dom.
IPR024253. Phosducin_thioredoxin-like_dom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF02114. Phosducin. 1 hit.
[Graphical view]
PRINTSPR00677. PHOSDUCIN.
SUPFAMSSF52833. SSF52833. 1 hit.
ProtoNetSearch...

Other

ChiTaRSPDCL. mouse.
NextBio324658.
PROQ9DBX2.
SOURCESearch...

Entry information

Entry namePHLP_MOUSE
AccessionPrimary (citable) accession number: Q9DBX2
Secondary accession number(s): Q3TKI0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot