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Protein

Cytochrome P450 4V2

Gene

Cyp4v2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Omega-hydroxylase that oxidizes medium-chain saturated fatty acids and polyunsaturated omega-3 fatty acids, and which plays a role in fatty acid and steroid metabolism in the eye. Catalyzes the omega-hydroxylation of medium-chain saturated fatty acids such as laurate, myristate and palmitate in an NADPH-dependent pathway. The substrate specificity is higher for myristate > laurate > palmitate (C14>C16>C12). Acts as a polyunsaturated omega-3 fatty acids hydroxylase by mediating oxidation of docosahexaenoate (DHA) to 22-hydroxydocosahexaenoate. Also produces some 21-hydroxydocosahexaenoate. Also converts eicosapentaenoate (EPA) to 20-hydroxyeicosapentaenoate (20-OH-EPA).By similarity

Catalytic activityi

Docosahexaenoate + NADPH + O2 = 22-hydroxydocosahexaenoate + NADP+ + H2O.By similarity

Cofactori

hemeBy similarity

Enzyme regulationi

Inhibited by N-hydroxy-N'-(4-n-butyl-2-methylphenyl formamidine)(HET0016) with an IC50 of 38 nM.By similarity

Pathwayi

Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei329 – 3291Heme (covalent; via 1 link)By similarity
Metal bindingi467 – 4671Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. monooxygenase activity Source: UniProtKB-KW
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Source: InterPro

GO - Biological processi

  1. fatty acid omega-oxidation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4V2 (EC:1.14.13.-)
Alternative name(s):
Docosahexaenoic acid omega-hydroxylase CYP4V2Curated (EC:1.14.13.199By similarity)
Gene namesi
Name:Cyp4v2
Synonyms:Cyp4v3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2142763. Cyp4v3.

Subcellular locationi

  1. Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Curated

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Cytochrome P450 4V2PRO_0000051861Add
BLAST

Proteomic databases

MaxQBiQ9DBW0.
PaxDbiQ9DBW0.
PRIDEiQ9DBW0.

PTM databases

PhosphoSiteiQ9DBW0.

Expressioni

Gene expression databases

BgeeiQ9DBW0.
ExpressionAtlasiQ9DBW0. baseline and differential.
GenevestigatoriQ9DBW0.

Interactioni

Protein-protein interaction databases

MINTiMINT-1863146.
STRINGi10090.ENSMUSP00000092966.

Structurei

3D structure databases

ProteinModelPortaliQ9DBW0.
SMRiQ9DBW0. Positions 62-520.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOVERGENiHBG000182.
InParanoidiQ9DBW0.
KOiK07427.
OMAiHEAIREE.
PhylomeDBiQ9DBW0.
TreeFamiTF105088.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DBW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLWLWLGLSG QKLLLWGAAS AVSLAGATIL ISIFPMLVSY ARKWQQMRSI
60 70 80 90 100
PSVARAYPLV GHALYMKPNN AEFFQQLIYY TEEFRHLPII KLWIGPVPLV
110 120 130 140 150
ALYKAENVEV ILTSSKQIDK SFLYKFLQPW LGLGLLTSTG SKWRTRRKML
160 170 180 190 200
TPTFHFTILE NFLDVMNEQA NILVNKLEKH VNQEAFNCFF YITLCALDII
210 220 230 240 250
CETAMGKNIG AQSNNDSEYV RTVYRMSDMI YRRMKMPWLW FDLWYLVFKE
260 270 280 290 300
GRDHKRGLKC LHTFTNNVIA ERVKERKAEE DWTGAGRGPI PSKNKRKAFL
310 320 330 340 350
DLLLSVTDEE GNRLSQEDIR EEVDTFMFEG HDTTAAAINW SLYLLGTNPE
360 370 380 390 400
VQRKVDQELD EVFGRSHRPV TLEDLKKLKY LDCVIKETLR VFPSVPLFAR
410 420 430 440 450
SLSEDCEVGG YKVTKGTEAI IIPYALHRDP RYFPDPEEFR PERFFPENSQ
460 470 480 490 500
GRHPYAYVPF SAGPRNCIGQ KFAVMEEKTI LACILRQFWV ESNQKREELG
510 520
LAGDLILRPN NGIWIKLKRR HEDDP
Length:525
Mass (Da):60,939
Last modified:June 1, 2001 - v1
Checksum:i71BB341589BD10A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056457 mRNA. Translation: BAB33032.1.
AK004724 mRNA. Translation: BAB23507.1.
CCDSiCCDS22276.1.
RefSeqiNP_598730.1. NM_133969.2.
UniGeneiMm.245297.

Genome annotation databases

EnsembliENSMUST00000095328; ENSMUSP00000092966; ENSMUSG00000079057.
GeneIDi102294.
KEGGimmu:102294.
UCSCiuc009lou.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056457 mRNA. Translation: BAB33032.1.
AK004724 mRNA. Translation: BAB23507.1.
CCDSiCCDS22276.1.
RefSeqiNP_598730.1. NM_133969.2.
UniGeneiMm.245297.

3D structure databases

ProteinModelPortaliQ9DBW0.
SMRiQ9DBW0. Positions 62-520.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1863146.
STRINGi10090.ENSMUSP00000092966.

PTM databases

PhosphoSiteiQ9DBW0.

Proteomic databases

MaxQBiQ9DBW0.
PaxDbiQ9DBW0.
PRIDEiQ9DBW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095328; ENSMUSP00000092966; ENSMUSG00000079057.
GeneIDi102294.
KEGGimmu:102294.
UCSCiuc009lou.1. mouse.

Organism-specific databases

CTDi102294.
MGIiMGI:2142763. Cyp4v3.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOVERGENiHBG000182.
InParanoidiQ9DBW0.
KOiK07427.
OMAiHEAIREE.
PhylomeDBiQ9DBW0.
TreeFamiTF105088.

Miscellaneous databases

NextBioi355392.
PROiQ9DBW0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DBW0.
ExpressionAtlasiQ9DBW0. baseline and differential.
GenevestigatoriQ9DBW0.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse mRNA for cytochrome P450, cDNA clone KK-1."
    Fujita Y., Kase K., Ohi H.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung.
  3. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 472-478, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiCP4V2_MOUSE
AccessioniPrimary (citable) accession number: Q9DBW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2005
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.