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Protein

Matrix-remodeling-associated protein 8

Gene

Mxra8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the maturation and maintenance of blood-brain barrier.1 Publication

GO - Biological processi

  • establishment of glial blood-brain barrier Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix-remodeling-associated protein 8
Alternative name(s):
Adipocyte-specific protein 3
Limitrin
Gene namesi
Name:Mxra8
Synonyms:Asp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1922011. Mxra8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 340ExtracellularSequence analysisAdd BLAST321
Transmembranei341 – 361HelicalSequence analysisAdd BLAST21
Topological domaini362 – 442CytoplasmicSequence analysisAdd BLAST81

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000029866620 – 442Matrix-remodeling-associated protein 8Add BLAST423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 136PROSITE-ProRule annotation
Glycosylationi118N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi185 ↔ 271PROSITE-ProRule annotation
Modified residuei227PhosphoserineBy similarity1
Glycosylationi305N-linked (GlcNAc...)Sequence analysis1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei324 – 325CleavageBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9DBV4.
PaxDbiQ9DBV4.
PRIDEiQ9DBV4.

PTM databases

iPTMnetiQ9DBV4.
PhosphoSitePlusiQ9DBV4.
SwissPalmiQ9DBV4.

Expressioni

Tissue specificityi

Expressed significantly in brain and localization is restricted to glia limitans which is formed by the endfeet of astrocytes surrounding capillaries and beneath the pia mater.1 Publication

Inductioni

Disruption of the blood-brain barrier by cold injury results in a drastic reduction in expression.1 Publication

Gene expression databases

BgeeiENSMUSG00000029070.
CleanExiMM_MXRA8.
ExpressionAtlasiQ9DBV4. baseline and differential.
GenevisibleiQ9DBV4. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9DBV4. 1 interactor.
MINTiMINT-4122441.
STRINGi10090.ENSMUSP00000030947.

Structurei

3D structure databases

ProteinModelPortaliQ9DBV4.
SMRiQ9DBV4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 156Ig-like V-type 1Add BLAST132
Domaini159 – 291Ig-like V-type 2Add BLAST133

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJVY. Eukaryota.
ENOG41113W9. LUCA.
GeneTreeiENSGT00390000001509.
HOGENOMiHOG000065729.
HOVERGENiHBG058527.
InParanoidiQ9DBV4.
OMAiLLTCVNR.
OrthoDBiEOG091G09TD.
PhylomeDBiQ9DBV4.
TreeFamiTF332884.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 2 hits.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DBV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLSRVLLW KLLLLQSSAV LSSGPSGTAA ASSSLVSESV VSLAAGTQAV
60 70 80 90 100
LRCQSPRMVW TQDRLHDRQR VVHWDLSGGP GSQRRRLVDM YSAGEQRVYE
110 120 130 140 150
PRDRDRLLLS PSAFHDGNFS LLIRAVDRGD EGVYTCNLHH HYCHLDESLA
160 170 180 190 200
VRLEVTEDPL LSRAYWDGEK EVLVVAHGAP ALMTCINRAH VWTDRHLEEA
210 220 230 240 250
QQVVHWDRQL PGVSHDRADR LLDLYASGER RAYGPPFLRD RVSVNTNAFA
260 270 280 290 300
RGDFSLRIDE LERADEGIYS CHLHHHYCGL HERRVFHLQV TEPAFEPPAR
310 320 330 340 350
ASPGNGSGHS SAPSPDPTLT RGHSIINVIV PEDHTHFFQQ LGYVLATLLL
360 370 380 390 400
FILLLITVVL ATRYRHSGGC KTSDKKAGKS KGKDVNMVEF AVATRDQAPY
410 420 430 440
RTEDIQLDYK NNILKERAEL AHSPLPAKDV DLDKEFRKEY CK
Length:442
Mass (Da):49,750
Last modified:June 1, 2001 - v1
Checksum:i9600816B14AAA6EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33S → N in BAD05133 (PubMed:14603461).Curated1
Sequence conflicti33S → N in BAB68501 (Ref. 2) Curated1
Sequence conflicti33S → N in AAH26438 (PubMed:15489334).Curated1
Sequence conflicti79G → D in BAD05133 (PubMed:14603461).Curated1
Sequence conflicti127D → E in BAD05133 (PubMed:14603461).Curated1
Sequence conflicti127D → E in BAB68501 (Ref. 2) Curated1
Sequence conflicti127D → E in AAH26438 (PubMed:15489334).Curated1
Sequence conflicti157E → D in BAD05133 (PubMed:14603461).Curated1
Sequence conflicti364Y → H in BAD05133 (PubMed:14603461).Curated1
Sequence conflicti364Y → H in BAB68501 (Ref. 2) Curated1
Sequence conflicti364Y → H in AAH26438 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052097 mRNA. Translation: BAD05133.1.
AB040488 mRNA. Translation: BAB68501.1.
AK004732 mRNA. Translation: BAB23514.1.
AL670236 Genomic DNA. Translation: CAM18391.1.
BC026438 mRNA. Translation: AAH26438.1.
CCDSiCCDS19044.1.
RefSeqiNP_077225.4. NM_024263.4.
UniGeneiMm.159028.

Genome annotation databases

EnsembliENSMUST00000030947; ENSMUSP00000030947; ENSMUSG00000029070.
GeneIDi74761.
KEGGimmu:74761.
UCSCiuc012dqy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052097 mRNA. Translation: BAD05133.1.
AB040488 mRNA. Translation: BAB68501.1.
AK004732 mRNA. Translation: BAB23514.1.
AL670236 Genomic DNA. Translation: CAM18391.1.
BC026438 mRNA. Translation: AAH26438.1.
CCDSiCCDS19044.1.
RefSeqiNP_077225.4. NM_024263.4.
UniGeneiMm.159028.

3D structure databases

ProteinModelPortaliQ9DBV4.
SMRiQ9DBV4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DBV4. 1 interactor.
MINTiMINT-4122441.
STRINGi10090.ENSMUSP00000030947.

PTM databases

iPTMnetiQ9DBV4.
PhosphoSitePlusiQ9DBV4.
SwissPalmiQ9DBV4.

Proteomic databases

MaxQBiQ9DBV4.
PaxDbiQ9DBV4.
PRIDEiQ9DBV4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030947; ENSMUSP00000030947; ENSMUSG00000029070.
GeneIDi74761.
KEGGimmu:74761.
UCSCiuc012dqy.1. mouse.

Organism-specific databases

CTDi54587.
MGIiMGI:1922011. Mxra8.

Phylogenomic databases

eggNOGiENOG410IJVY. Eukaryota.
ENOG41113W9. LUCA.
GeneTreeiENSGT00390000001509.
HOGENOMiHOG000065729.
HOVERGENiHBG058527.
InParanoidiQ9DBV4.
OMAiLLTCVNR.
OrthoDBiEOG091G09TD.
PhylomeDBiQ9DBV4.
TreeFamiTF332884.

Miscellaneous databases

PROiQ9DBV4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029070.
CleanExiMM_MXRA8.
ExpressionAtlasiQ9DBV4. baseline and differential.
GenevisibleiQ9DBV4. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 2 hits.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMXRA8_MOUSE
AccessioniPrimary (citable) accession number: Q9DBV4
Secondary accession number(s): Q76M97, Q920S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.