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Protein

E3 ubiquitin-protein ligase RNF6

Gene

Rnf6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri614 – 655RING-type; atypicalPROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • androgen receptor binding Source: UniProtKB
  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF6 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase RNF6Curated
RLIM-like protein
Gene namesi
Name:Rnf6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1921382 Rnf6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003957511 – 667E3 ubiquitin-protein ligase RNF6Add BLAST667

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei559PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9DBU5
MaxQBiQ9DBU5
PaxDbiQ9DBU5
PRIDEiQ9DBU5

PTM databases

iPTMnetiQ9DBU5
PhosphoSitePlusiQ9DBU5

Expressioni

Tissue specificityi

Widely expressed with higher expression in the testis in both germ cells and Sertoli cells.1 Publication

Developmental stagei

Expressed in the floor plate and ventral portions of the developing neural tube of E12.5 to E13 mouse embryos. Found in developing neuronal projections (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000029634
ExpressionAtlasiQ9DBU5 baseline and differential
GenevisibleiQ9DBU5 MM

Interactioni

Subunit structurei

Interacts with AR (By similarity). Interacts with LIMK1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi216515, 2 interactors
IntActiQ9DBU5, 2 interactors
MINTiQ9DBU5
STRINGi10090.ENSMUSP00000067559

Structurei

3D structure databases

ProteinModelPortaliQ9DBU5
SMRiQ9DBU5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni589 – 667Required for polyubiquitinationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi174 – 410Arg-richAdd BLAST237

Sequence similaritiesi

Belongs to the RNF12 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri614 – 655RING-type; atypicalPROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0800 Eukaryota
ENOG41121N2 LUCA
GeneTreeiENSGT00730000110459
HOGENOMiHOG000273881
HOVERGENiHBG009886
InParanoidiQ9DBU5
OMAiHVWENGA
OrthoDBiEOG091G04S3
PhylomeDBiQ9DBU5
TreeFamiTF325756

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF13639 zf-RING_2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DBU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPSRSRSGG SGEESSFQEN ERRWQQERLH REEAYYQFIN ELSDEDYRLM
60 70 80 90 100
RDHNLLGTPG EITSEELQQR LERAKEQLAS QPGSDSAASD GDSESLRAHS
110 120 130 140 150
DEDSLLRWLN TFRRTGNVTR SGQNGNQSWR AVSRTNPNSG EFGFSLEIHI
160 170 180 190 200
NPDNRGSEMH GEDSTDIPLS GVNREHRQQR PSSPVARRTR SQTSMSSSGP
210 220 230 240 250
RGRRGARRQG SVQGSFATLG RLRNGIGVAL GVPRVSAPRT NVINSHTNQS
260 270 280 290 300
DGSTLRQGGR QRFGAAHIWE NGARSNVTVR NTNQRLEPIR LRPAFSSRSR
310 320 330 340 350
SPIQRQNGTV HHNSQRQGRP VQQTGRNRSV RHRGVTRVFL EQGREHRGTD
360 370 380 390 400
YTPLSNSRLV SRITVEEGES SRSSAATQRH PAITLDLQVR RIRPGETRDR
410 420 430 440 450
DSIANRTRSR AGLAESTVES TSGGFHRTIS HLERSGVRTY VSTITVPLRR
460 470 480 490 500
ISENDVVEPS SVALRSILRQ IMTGFGELSS LMEVEPASEN QSNGQRLPEV
510 520 530 540 550
YLELSNGDAA DDSGQHGRAS SQASQAQDGA EMLAVREPAP PQARPSGSRS
560 570 580 590 600
RRQLGRADSV VEAGTLPILR LAHFFLLNEG DDDPIRGLTK EQIDNLSTRS
610 620 630 640 650
YEQDGVDSEL GKVCSVCISD YVAGNKLRQL PCLHEFHIHC IDRWLSENCT
660
CPVCRRPVLE FGATSSG
Length:667
Mass (Da):74,091
Last modified:June 1, 2001 - v1
Checksum:i66B26D8DC20D571A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3P → L in AAK84435 (PubMed:11971979).Curated1
Sequence conflicti3P → L in EDL05801 (Ref. 3) Curated1
Sequence conflicti3P → L in EDL05802 (Ref. 3) Curated1
Sequence conflicti346H → R in AAK84435 (PubMed:11971979).Curated1
Sequence conflicti346H → R in EDL05801 (Ref. 3) Curated1
Sequence conflicti346H → R in EDL05802 (Ref. 3) Curated1
Sequence conflicti346H → R in AAI38546 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039004 mRNA Translation: AAK84435.1
AK004745 mRNA Translation: BAB23526.1
AK150269 mRNA Translation: BAE29425.1
AK152106 mRNA Translation: BAE30953.1
CH466614 Genomic DNA Translation: EDL05801.1
CH466614 Genomic DNA Translation: EDL05802.1
BC138545 mRNA Translation: AAI38546.1
CCDSiCCDS19868.1
RefSeqiNP_001243014.1, NM_001256085.1
NP_001243016.1, NM_001256087.1
NP_083050.1, NM_028774.3
UniGeneiMm.26696

Genome annotation databases

EnsembliENSMUST00000067837; ENSMUSP00000067559; ENSMUSG00000029634
ENSMUST00000161859; ENSMUSP00000124293; ENSMUSG00000029634
ENSMUST00000169407; ENSMUSP00000128774; ENSMUSG00000029634
GeneIDi74132
KEGGimmu:74132
UCSCiuc009anb.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRNF6_MOUSE
AccessioniPrimary (citable) accession number: Q9DBU5
Secondary accession number(s): B2RRR0, Q8K565
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: June 1, 2001
Last modified: February 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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