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Protein

Serine/threonine-protein kinase RIO3

Gene

Riok3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway. May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei290ATPBy similarity1
Active sitei406Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi257 – 265ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processAntiviral defense, Immunity, Innate immunity, Ribosome biogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase RIO3 (EC:2.7.11.1)
Alternative name(s):
RIO kinase 3
Gene namesi
Name:Riok3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1914128. Riok3.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135301 – 519Serine/threonine-protein kinase RIO3Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineBy similarity1
Modified residuei112PhosphoserineBy similarity1
Modified residuei125PhosphoserineCombined sources1
Modified residuei127PhosphoserineCombined sources1
Modified residuei128PhosphoserineCombined sources1
Modified residuei512PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated (in vitro).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9DBU3.
MaxQBiQ9DBU3.
PaxDbiQ9DBU3.
PRIDEiQ9DBU3.

PTM databases

iPTMnetiQ9DBU3.
PhosphoSitePlusiQ9DBU3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024404.
CleanExiMM_RIOK3.
GenevisibleiQ9DBU3. MM.

Interactioni

Subunit structurei

Interacts with CASP10. Interacts with IRF3; RIOK3 probybly mediates the interaction of TBK1 with IRF3. Associated with 40S pre-ribosomal particles.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211783. 1 interactor.
STRINGi10090.ENSMUSP00000025270.

Structurei

3D structure databases

ProteinModelPortaliQ9DBU3.
SMRiQ9DBU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini251 – 519Protein kinaseAdd BLAST269

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2269. Eukaryota.
COG1718. LUCA.
GeneTreeiENSGT00390000004370.
HOGENOMiHOG000007623.
HOVERGENiHBG057475.
InParanoidiQ9DBU3.
KOiK08872.
OMAiKMNEDHV.
OrthoDBiEOG091G064C.
TreeFamiTF105831.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000687. RIO_kinase.
IPR018935. RIO_kinase_CS.
IPR017406. Ser/Thr_kinase_Rio3.
PANTHERiPTHR10593:SF75. PTHR10593:SF75. 1 hit.
PIRSFiPIRSF038146. Ser/Thr_PK_RIO3. 1 hit.
SMARTiView protein in SMART
SM00090. RIO. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01245. RIO1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DBU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLVGVSSPE PGPAAAWGPS KCPWATPQNT VSCSLTEVMS EELAKELQLE
60 70 80 90 100
EEAAAFPEVV VAEGPFISGE NIDTSSDLML AQMLQMEFDR EYDAQLRREE
110 120 130 140 150
KKFNGDSKVS ISFENYRKVH PFEDSDSSED EVDWQDTRDD PYRPAKPIPT
160 170 180 190 200
PKKGFIGKGK DITTKHDEVV CGRKNTARME NFAPGFQVGD GIGMDLKLSN
210 220 230 240 250
HVFNALKQHA YSEERRSARL HEKKEHSTAE KAVDPKTRLL MYKMVNSGML
260 270 280 290 300
ETITGCISTG KESVVFHAYG GSLEDEKEDG KAIPTECAIK VFKTTLNEFK
310 320 330 340 350
NRDKYIKDDF RFKDRFSKLN PRKIIRMWAE KEMHNLTRMQ KAGIPCPTVV
360 370 380 390 400
LLKKHILVMS FIGHDQVPAP KLKEVKLSNE EMKDAYYQTL HLMQQLYNEC
410 420 430 440 450
TLVHADLSEY NMLWHAGKVW LIDVSQSVEP THPHGLEFLF RDCRNVSQFF
460 470 480 490 500
QKGGVTEALN ERELFNAVSG LNISADNEAD FLAEIEALEK MNEDHIQKNG
510
RKAASFLKDD GSPPVLSAD
Length:519
Mass (Da):58,704
Last modified:July 27, 2011 - v3
Checksum:i4DB90CAFA81C8AC7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti141P → T in BAB23529 (PubMed:16141072).Curated1
Sequence conflicti496I → V in AAH33271 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004748 mRNA. Translation: BAB23529.1.
AK147074 mRNA. Translation: BAE27655.1.
BC033271 mRNA. Translation: AAH33271.1.
CH466622 Genomic DNA. Translation: EDL01557.1.
CCDSiCCDS29062.1.
RefSeqiNP_077144.2. NM_024182.4.
UniGeneiMm.28551.

Genome annotation databases

EnsembliENSMUST00000025270; ENSMUSP00000025270; ENSMUSG00000024404.
GeneIDi66878.
KEGGimmu:66878.
UCSCiuc008eby.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiRIOK3_MOUSE
AccessioniPrimary (citable) accession number: Q9DBU3
Secondary accession number(s): Q3UI51, Q8CIC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 27, 2011
Last modified: September 27, 2017
This is version 113 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families