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Protein

N-alpha-acetyltransferase 60

Gene

Naa60

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol. Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase. May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences.By similarity

Catalytic activityi

Acetyl-CoA + an N-terminal-L-methionyl-[transmembrane protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-[transmembrane protein] + CoA.By similarity
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38SubstrateBy similarity1
Active sitei97By similarity1
Binding sitei99Substrate; via carbonyl oxygenBy similarity1
Active sitei138By similarity1
Binding sitei143Acetyl-CoABy similarity1
Binding sitei165SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Chromatin regulator, Transferase
Biological processChromosome partition

Names & Taxonomyi

Protein namesi
Recommended name:
N-alpha-acetyltransferase 60 (EC:2.3.1.259By similarity)
Alternative name(s):
Histone acetyltransferase type B protein 4By similarity (EC:2.3.1.48By similarity)
Short name:
HAT4By similarity
N-acetyltransferase 15
N-alpha-acetyltransferase F
Short name:
NatF
Gene namesi
Name:Naa60
Synonyms:Nat15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1922013 Naa60

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 192CytoplasmicBy similarityAdd BLAST192
Intramembranei193 – 236HelicalBy similarityAdd BLAST44
Topological domaini237 – 242CytoplasmicBy similarity6

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003215671 – 242N-alpha-acetyltransferase 60Add BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79N6-acetyllysine; by autocatalysisBy similarity1
Modified residuei105N6-acetyllysine; by autocatalysisBy similarity1
Modified residuei109N6-acetyllysine; by autocatalysisBy similarity1
Modified residuei121N6-acetyllysine; by autocatalysisBy similarity1

Post-translational modificationi

Acetylated: autoacetylation is required for optimal acetyltransferase activity.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9DBU2
PaxDbiQ9DBU2
PRIDEiQ9DBU2

PTM databases

iPTMnetiQ9DBU2
PhosphoSitePlusiQ9DBU2

Expressioni

Gene expression databases

BgeeiENSMUSG00000005982
ExpressionAtlasiQ9DBU2 baseline and differential
GenevisibleiQ9DBU2 MM

Interactioni

Subunit structurei

Monomer and homodimer; monomer in presence of substrate and homodimer in its absence.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000006138

Structurei

3D structure databases

ProteinModelPortaliQ9DBU2
SMRiQ9DBU2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 182N-acetyltransferasePROSITE-ProRule annotationAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 103Acetyl-CoA bindingBy similarity3
Regioni109 – 114Acetyl-CoA bindingBy similarity6
Regioni150 – 153Acetyl-CoA bindingBy similarity4
Regioni162 – 173Required for homodimerizationBy similarityAdd BLAST12

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IQK0 Eukaryota
ENOG410Y94A LUCA
GeneTreeiENSGT00390000008314
HOGENOMiHOG000280717
HOVERGENiHBG061680
InParanoidiQ9DBU2
KOiK21121
OMAiSIPQRLY
OrthoDBiEOG091G0L3L
TreeFamiTF323980

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DBU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEVVPSSAL SEVSLRLLCH DDIDTVKHLC GDWFPIEYPD SWYRDITSNK
60 70 80 90 100
KFFSLAATYR GAIVGMIVAE IKNRTKIHKE DGDILASSFS VDTQVAYILS
110 120 130 140 150
LGVVKEFRKH GIGSLLLESL KDHISTTAQD HCKAIYLHVL TTNNTAINFY
160 170 180 190 200
ENRDFRQHHY LPYYYSIRGV LKDGFTYVLY INGGHPPWTI LDYIQHLGSA
210 220 230 240
LANLSPCSIP HRIYRQAHSL LCSFLPWSSI STKGGIEYSR TM
Length:242
Mass (Da):27,507
Last modified:June 1, 2001 - v1
Checksum:iF2307B2007F13F84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004750 mRNA Translation: BAB23531.1
AK088736 mRNA Translation: BAC40538.1
BC004837 mRNA Translation: AAH04837.1
CCDSiCCDS37239.1
RefSeqiNP_001277618.1, NM_001290689.1
NP_083366.1, NM_029090.3
XP_006522742.1, XM_006522679.1
XP_006522743.1, XM_006522680.2
UniGeneiMm.28403

Genome annotation databases

EnsembliENSMUST00000006138; ENSMUSP00000006138; ENSMUSG00000005982
ENSMUST00000115860; ENSMUSP00000111526; ENSMUSG00000005982
ENSMUST00000150655; ENSMUSP00000135206; ENSMUSG00000005982
ENSMUST00000186375; ENSMUSP00000140031; ENSMUSG00000005982
GeneIDi74763
KEGGimmu:74763
UCSCiuc007xyw.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNAA60_MOUSE
AccessioniPrimary (citable) accession number: Q9DBU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2001
Last modified: February 28, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome