Reviewed, UniProtKB/Swiss-Prot Q9DBT9 (M2GD_MOUSE)

Names and origin

Protein names

Recommended name:
    Dimethylglycine dehydrogenase, mitochondrial
    EC=1.5.99.2
Alternative name(s):
    ME2GLYDH

Gene names

Name:

Dmgdh

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	869 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	N,N-dimethylglycine + acceptor + H2O = sarcosine + formaldehyde + reduced acceptor.
Cofactor	Binds 1 FAD covalently per monomer By similarity.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 2/2.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: MGI
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: InterPro dimethylglycine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Mitochondrion Potential
	Chain	44 – 869	826	Dimethylglycine dehydrogenase, mitochondrial		PRO_0000010768
Amino acid modifications
	Modified residue	84	1	Pros-8alpha-FAD histidine By similarity
	Modified residue	866	1	Phosphothreonine Ref.3
Experimental info
	Sequence conflict	758	1	T → A in AAH24126. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9DBT9-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D415C743105A0547	FASTA	869	97,255
	10 20 30 40 50 60 MLRPGALRLR GLALRGSPRR PSSAGLREGQ ESPASPPEWK DRAETVIIGG GCVGVSLAYH 70 80 90 100 110 120 LAKAGMRDVV LMEKSELTAG STWHAAGLTT YFHPGINLKK IHYDSIKLYE RLEEETGQVV 130 140 150 160 170 180 GFHQPGSIRL ATTPVRVDEF KYQMTRTNWH ATEQYIIEPE KIHELFPLLN MNKILAGLYN 190 200 210 220 230 240 PGDGHIDPYS LTMALAAGAR KYGALLKYPA PVTSLKPRPD GTWDVETPQG SVRANRIVNA 250 260 270 280 290 300 AGFWAREVGK MIGLDHPLIP VQHQYVVTST IPEVKALKRE LPVLRDLEGS YYLRQERDGL 310 320 330 340 350 360 LFGPYESQEK MKLQASWVTH GVPPGFGKEL FESDLDRISD HLEAAMEMIP VLKKADIINV 370 380 390 400 410 420 VNGPITYSPD ILPMVGPHQG VRNYWVATGF GYGIIHAGGV GKFLSDWILH GEPPFDLIEL 430 440 450 460 470 480 DPNRYGKWTT TQYTEAKARE SYGFNNIVGY PKEERFAGRP TQRVSGLYKT LKSKCSMGFH 490 500 510 520 530 540 AGWEQPHWFY KPGQDTQYRP SFRRTNWFEP VGSEYKQVMQ RVGVIDLSPF GKFNIKGRDS 550 560 570 580 590 600 TQLLDHLFAN VIPKVGFTNI SHMLTPRGRV YAELTVSQQS PGEFLLITGS GSELHDLRWI 610 620 630 640 650 660 EEAAFRGGYD VEIQNITDEF GVLGVAGPYA RRVLQKLTSE DLSDDAFKFL QTKSFNISDI 670 680 690 700 710 720 PVTAIRISYT GELGWELYHR REDSATLYER IMSAGQEEGI GDFGTYALNA LRLEKAFRAW 730 740 750 760 770 780 GSEMNCDTNP LEAGLEYFVK LNKPADFIGK QALKQIKTEG LKRRLVCLTV ATDDVDPEGN 790 800 810 820 830 840 ESIWYKGKVV GNTTSGSYSY SIQKSLAFAY VPVQLSEVGQ QVEVELLGKN YPATIIQEPL 850 860 VLTEPARARL QKDGKKTNLE KGPSRTTKL

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 237-869. Tissue: Liver.
[3]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed: 17622165] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-866, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK004755 mRNA. Translation: BAB23536.1. BC024126 mRNA. Translation: AAH24126.1.
IPI	IPI00120123.
RefSeq	NP_083048.1.
UniGene	Mm.21789
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000042102. Mus musculus. [Contig view]
GeneID	74129.
KEGG	mmu:74129.
Organism-specific databases
MGI	MGI:1921379. Dmgdh.
Phylogenomic databases
HOGENOM	Q9DBT9.
HOVERGEN	Q9DBT9.
OMA	Q9DBT9. REVGKMI.
Enzyme and pathway databases
BRENDA	1.5.99.2. 244.
Gene expression databases
ArrayExpress	Q9DBT9.
Bgee	Q9DBT9.
CleanEx	MM_DMGDH.
GermOnline	ENSMUSG00000042102. Mus musculus.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR013977. GCV_T_C. IPR006222. GCV_T_N. [Graphical view]
Pfam	PF01266. DAO. 1 hit. PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	339854.
SOURCE	Search...

Entry information

Entry name

M2GD_MOUSE

Accession

Primary (citable) accession number: Q9DBT9
Secondary accession number(s): Q8R1S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 1, 2001
Last modified:	May 5, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)