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Q9DBT9 (M2GD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dimethylglycine dehydrogenase, mitochondrial
EC=1.5.99.2
Alternative name(s):
ME2GLYDH
Gene names
Name:Dmgdh
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length869 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

N,N-dimethylglycine + acceptor + H2O = sarcosine + formaldehyde + reduced acceptor.

Cofactor

Binds 1 FAD covalently per monomer By similarity.

Pathway

Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 2/2.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the GcvT family.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processglycine catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentmitochondrion

Inferred from direct assay. Source: MGI

   Molecular functionaminomethyltransferase activity

Inferred from electronic annotation. Source: InterPro

dimethylglycine dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Mitochondrion Potential
Chain44 – 869826Dimethylglycine dehydrogenase, mitochondrial
PRO_0000010768

Amino acid modifications

Modified residue841Tele-8alpha-FAD histidine By similarity
Modified residue8661Phosphothreonine Ref.4

Experimental info

Sequence conflict7581T → A in AAH24126. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9DBT9 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: D415C743105A0547

FASTA86997,255
        10         20         30         40         50         60 
MLRPGALRLR GLALRGSPRR PSSAGLREGQ ESPASPPEWK DRAETVIIGG GCVGVSLAYH 

        70         80         90        100        110        120 
LAKAGMRDVV LMEKSELTAG STWHAAGLTT YFHPGINLKK IHYDSIKLYE RLEEETGQVV 

       130        140        150        160        170        180 
GFHQPGSIRL ATTPVRVDEF KYQMTRTNWH ATEQYIIEPE KIHELFPLLN MNKILAGLYN 

       190        200        210        220        230        240 
PGDGHIDPYS LTMALAAGAR KYGALLKYPA PVTSLKPRPD GTWDVETPQG SVRANRIVNA 

       250        260        270        280        290        300 
AGFWAREVGK MIGLDHPLIP VQHQYVVTST IPEVKALKRE LPVLRDLEGS YYLRQERDGL 

       310        320        330        340        350        360 
LFGPYESQEK MKLQASWVTH GVPPGFGKEL FESDLDRISD HLEAAMEMIP VLKKADIINV 

       370        380        390        400        410        420 
VNGPITYSPD ILPMVGPHQG VRNYWVATGF GYGIIHAGGV GKFLSDWILH GEPPFDLIEL 

       430        440        450        460        470        480 
DPNRYGKWTT TQYTEAKARE SYGFNNIVGY PKEERFAGRP TQRVSGLYKT LKSKCSMGFH 

       490        500        510        520        530        540 
AGWEQPHWFY KPGQDTQYRP SFRRTNWFEP VGSEYKQVMQ RVGVIDLSPF GKFNIKGRDS 

       550        560        570        580        590        600 
TQLLDHLFAN VIPKVGFTNI SHMLTPRGRV YAELTVSQQS PGEFLLITGS GSELHDLRWI 

       610        620        630        640        650        660 
EEAAFRGGYD VEIQNITDEF GVLGVAGPYA RRVLQKLTSE DLSDDAFKFL QTKSFNISDI 

       670        680        690        700        710        720 
PVTAIRISYT GELGWELYHR REDSATLYER IMSAGQEEGI GDFGTYALNA LRLEKAFRAW 

       730        740        750        760        770        780 
GSEMNCDTNP LEAGLEYFVK LNKPADFIGK QALKQIKTEG LKRRLVCLTV ATDDVDPEGN 

       790        800        810        820        830        840 
ESIWYKGKVV GNTTSGSYSY SIQKSLAFAY VPVQLSEVGQ QVEVELLGKN YPATIIQEPL 

       850        860 
VLTEPARARL QKDGKKTNLE KGPSRTTKL

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Lung.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 237-869.
Tissue: Liver.
[4]"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells."
Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.
J. Proteome Res. 6:3174-3186(2007) [PubMed: 17622165] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-866, MASS SPECTROMETRY.
Tissue: Teratocarcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK004755 mRNA. Translation: BAB23536.1.
CT030023, AC131739 Genomic DNA. Translation: CAO77970.1.
BC024126 mRNA. Translation: AAH24126.1.
IPIIPI00120123.
RefSeqNP_083048.1. NM_028772.3.
UniGeneMm.21789.

3D structure databases

ProteinModelPortalQ9DBT9.
SMRQ9DBT9. Positions 41-849.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9DBT9.

PTM databases

PhosphoSiteQ9DBT9.

Proteomic databases

PRIDEQ9DBT9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000048001; ENSMUSP00000039663; ENSMUSG00000042102.
GeneID74129.
KEGGmmu:74129.
UCSCuc007rll.1. mouse.

Organism-specific databases

CTD29958.
MGIMGI:1921379. Dmgdh.

Phylogenomic databases

GeneTreeENSGT00530000063120.
HOGENOMHBG322775.
HOVERGENHBG081945.
InParanoidQ9DBT9.
OMAREVGKMI.
OrthoDBEOG466VK7.
PhylomeDBQ9DBT9.

Gene expression databases

ArrayExpressQ9DBT9.
BgeeQ9DBT9.
CleanExMM_DMGDH.
GenevestigatorQ9DBT9.
GermOnlineENSMUSG00000042102. Mus musculus.

Family and domain databases

InterProIPR006076. FAD-dep_OxRdtase.
IPR013977. GCV_T_C.
IPR006222. GCV_T_N.
[Graphical view]
KOK00315.
PfamPF01266. DAO. 1 hit.
PF01571. GCV_T. 1 hit.
PF08669. GCV_T_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio339854.
SOURCESearch...

Entry information

Entry nameM2GD_MOUSE
AccessionPrimary (citable) accession number: Q9DBT9
Secondary accession number(s): B1B1D0, Q8R1S7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 1, 2001
Last modified: November 16, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents