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Protein

Ectonucleoside triphosphate diphosphohydrolase 4

Gene

Entpd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes preferentially nucleoside 5'-diphosphates, nucleoside 5'-triphosphates are hydrolyzed only to a minor extent.By similarity

Catalytic activityi

A nucleoside diphosphate + H2O = a nucleoside phosphate + phosphate.

Cofactori

Ca2+By similarity, Mg2+By similarityNote: Requires Ca2+ and can also use Mg2+, but with lower efficiency.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei222Proton acceptorBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandCalcium, Magnesium

Enzyme and pathway databases

ReactomeiR-MMU-8850843 Phosphate bond hydrolysis by NTPDase proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 4 (EC:3.6.1.6)
Short name:
NTPDase 4
Alternative name(s):
Lysosomal apyrase-like protein of 70 kDa
Uridine-diphosphatase
Short name:
UDPase
Gene namesi
Name:Entpd4
Synonyms:Lalp70, Lysal1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1914714 Entpd4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 559LumenalSequence analysisAdd BLAST505
Transmembranei560 – 580HelicalSequence analysisAdd BLAST21
Topological domaini581 – 613CytoplasmicSequence analysisAdd BLAST33

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002099121 – 613Ectonucleoside triphosphate diphosphohydrolase 4Add BLAST613

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi404N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi407N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi461 ↔ 490By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9DBT4
PRIDEiQ9DBT4

PTM databases

PhosphoSitePlusiQ9DBT4

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000022066
CleanExiMM_ENTPD4
ExpressionAtlasiQ9DBT4 baseline
GenevisibleiQ9DBT4 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065046

Structurei

3D structure databases

ProteinModelPortaliQ9DBT4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1386 Eukaryota
COG5371 LUCA
GeneTreeiENSGT00550000074435
HOGENOMiHOG000090205
HOVERGENiHBG006789
InParanoidiQ9DBT4
KOiK12305
OMAiSEYWYTA
OrthoDBiEOG091G04IV
PhylomeDBiQ9DBT4
TreeFamiTF354343

Family and domain databases

InterProiView protein in InterPro
IPR000407 GDA1_CD39_NTPase
PANTHERiPTHR11782 PTHR11782, 1 hit
PfamiView protein in Pfam
PF01150 GDA1_CD39, 1 hit
PROSITEiView protein in PROSITE
PS01238 GDA1_CD39_NTPASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DBT4-1) [UniParc]FASTAAdd to basket
Also known as: LALP70

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRIGISCLF PASWHFSISP VGCPRILNTN LRQIVVISIL AAAVSLLYFS
60 70 80 90 100
VVIIRSKYGW LSKDKKFQRY LARVTDVEAT DTNNPSVNYG IVVDCGSSGS
110 120 130 140 150
RIFVYCWPRH NGNPHDLLDI RQMRDKNRKP VVMKIKPGIS EFATSPEKVS
160 170 180 190 200
DYISPLLSFA AEHVPRAKHK ETPLYILCTA GMRVLPESQQ KAILEDLLTD
210 220 230 240 250
IPVHYDFLFS DSHAEVISGK QEGVYAWIGI NFVLGRFEHI EEDDEAVVEV
260 270 280 290 300
NIPGSESSEA IVRKRTAGVL DMGGVSTQIA YEVPQTVSFA SSQQEEVAKN
310 320 330 340 350
LLAEFNLGCD VHQTEHVYRV YVATFLGFGG NAARQRYEDR LFASTVQKNR
360 370 380 390 400
LLGKQTGLTP DAPLLDPCLP LDIKDEIQQN GQTLYLQGTG DFDLCRETLQ
410 420 430 440 450
PFMNKTNETQ TSLNGVYQPP IHFQNSEFYG FSEFYYCTED VLRMGGDYNA
460 470 480 490 500
ARFTQAAKDY CATKWSILRE RFDRGLYASH ADLHRLKYQC FKSAWMFEVF
510 520 530 540 550
HKGFSFPVTY KNLKTALQVY DKEVQWTLGA ILYRTRFLPL RDIRQEVFRA
560 570 580 590 600
GHAHWRGVSF VYNHYLFSGC FLVVLLSILL YLLRLRRIHR RAPRTGSLWM
610
EEGLPSQKGP GPL
Length:613
Mass (Da):69,746
Last modified:June 1, 2001 - v1
Checksum:iDE528F512ABEF52F
GO
Isoform 2 (identifier: Q9DBT4-2) [UniParc]FASTAAdd to basket
Also known as: LALP70V

The sequence of this isoform differs from the canonical sequence as follows:
     287-294: Missing.

Show »
Length:605
Mass (Da):68,911
Checksum:i181F561B64BC62FC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003615287 – 294Missing in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004761 mRNA Translation: BAB23542.1
BC006924 mRNA Translation: AAH06924.1
BC043134 mRNA Translation: AAH43134.1
CCDSiCCDS27240.1 [Q9DBT4-1]
RefSeqiNP_080450.1, NM_026174.3 [Q9DBT4-1]
XP_003688976.1, XM_003688928.3
XP_003688977.1, XM_003688929.4
XP_006519516.1, XM_006519453.2 [Q9DBT4-2]
UniGeneiMm.489765

Genome annotation databases

EnsembliENSMUST00000064831; ENSMUSP00000065046; ENSMUSG00000095463 [Q9DBT4-1]
ENSMUST00000184973; ENSMUSP00000138944; ENSMUSG00000095463 [Q9DBT4-1]
ENSMUST00000185072; ENSMUSP00000139202; ENSMUSG00000022066 [Q9DBT4-1]
GeneIDi100862375
67464
KEGGimmu:67464
UCSCiuc007umi.2 mouse [Q9DBT4-1]
uc007umk.2 mouse [Q9DBT4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiENTP4_MOUSE
AccessioniPrimary (citable) accession number: Q9DBT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome