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Protein

Ectonucleoside triphosphate diphosphohydrolase 4

Gene

Entpd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes preferentially nucleoside 5'-diphosphates, nucleoside 5'-triphosphates are hydrolyzed only to a minor extent.By similarity

Catalytic activityi

A nucleoside diphosphate + H2O = a nucleoside phosphate + phosphate.

Cofactori

Ca2+By similarity, Mg2+By similarityNote: Requires Ca2+ and can also use Mg2+, but with lower efficiency.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei222 – 2221Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 4 (EC:3.6.1.6)
Short name:
NTPDase 4
Alternative name(s):
Lysosomal apyrase-like protein of 70 kDa
Uridine-diphosphatase
Short name:
UDPase
Gene namesi
Name:Entpd4
Synonyms:Lalp70, Lysal1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1914714. Entpd4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence analysisAdd
BLAST
Transmembranei34 – 5421HelicalSequence analysisAdd
BLAST
Topological domaini55 – 559505LumenalSequence analysisAdd
BLAST
Transmembranei560 – 58021HelicalSequence analysisAdd
BLAST
Topological domaini581 – 61333CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 613613Ectonucleoside triphosphate diphosphohydrolase 4PRO_0000209912Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi404 – 4041N-linked (GlcNAc...)Sequence analysis
Glycosylationi407 – 4071N-linked (GlcNAc...)Sequence analysis
Disulfide bondi461 ↔ 490By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9DBT4.
PaxDbiQ9DBT4.
PRIDEiQ9DBT4.

PTM databases

PhosphoSiteiQ9DBT4.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiQ9DBT4.
CleanExiMM_ENTPD4.
ExpressionAtlasiQ9DBT4. baseline.
GenevisibleiQ9DBT4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065046.

Structurei

3D structure databases

ProteinModelPortaliQ9DBT4.
SMRiQ9DBT4. Positions 87-539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1386. Eukaryota.
COG5371. LUCA.
GeneTreeiENSGT00550000074435.
HOGENOMiHOG000090205.
HOVERGENiHBG006789.
InParanoidiQ9DBT4.
KOiK12305.
OMAiNSQFYGF.
OrthoDBiEOG793B78.
PhylomeDBiQ9DBT4.
TreeFamiTF354343.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 3 hits.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DBT4-1) [UniParc]FASTAAdd to basket

Also known as: LALP70

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRIGISCLF PASWHFSISP VGCPRILNTN LRQIVVISIL AAAVSLLYFS
60 70 80 90 100
VVIIRSKYGW LSKDKKFQRY LARVTDVEAT DTNNPSVNYG IVVDCGSSGS
110 120 130 140 150
RIFVYCWPRH NGNPHDLLDI RQMRDKNRKP VVMKIKPGIS EFATSPEKVS
160 170 180 190 200
DYISPLLSFA AEHVPRAKHK ETPLYILCTA GMRVLPESQQ KAILEDLLTD
210 220 230 240 250
IPVHYDFLFS DSHAEVISGK QEGVYAWIGI NFVLGRFEHI EEDDEAVVEV
260 270 280 290 300
NIPGSESSEA IVRKRTAGVL DMGGVSTQIA YEVPQTVSFA SSQQEEVAKN
310 320 330 340 350
LLAEFNLGCD VHQTEHVYRV YVATFLGFGG NAARQRYEDR LFASTVQKNR
360 370 380 390 400
LLGKQTGLTP DAPLLDPCLP LDIKDEIQQN GQTLYLQGTG DFDLCRETLQ
410 420 430 440 450
PFMNKTNETQ TSLNGVYQPP IHFQNSEFYG FSEFYYCTED VLRMGGDYNA
460 470 480 490 500
ARFTQAAKDY CATKWSILRE RFDRGLYASH ADLHRLKYQC FKSAWMFEVF
510 520 530 540 550
HKGFSFPVTY KNLKTALQVY DKEVQWTLGA ILYRTRFLPL RDIRQEVFRA
560 570 580 590 600
GHAHWRGVSF VYNHYLFSGC FLVVLLSILL YLLRLRRIHR RAPRTGSLWM
610
EEGLPSQKGP GPL
Length:613
Mass (Da):69,746
Last modified:June 1, 2001 - v1
Checksum:iDE528F512ABEF52F
GO
Isoform 2 (identifier: Q9DBT4-2) [UniParc]FASTAAdd to basket

Also known as: LALP70V

The sequence of this isoform differs from the canonical sequence as follows:
     287-294: Missing.

Show »
Length:605
Mass (Da):68,911
Checksum:i181F561B64BC62FC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei287 – 2948Missing in isoform 2. 1 PublicationVSP_003615

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004761 mRNA. Translation: BAB23542.1.
BC006924 mRNA. Translation: AAH06924.1.
BC043134 mRNA. Translation: AAH43134.1.
CCDSiCCDS27240.1. [Q9DBT4-1]
RefSeqiNP_080450.1. NM_026174.3. [Q9DBT4-1]
XP_003688976.1. XM_003688928.2. [Q9DBT4-1]
XP_003688977.1. XM_003688929.4. [Q9DBT4-2]
XP_006519516.1. XM_006519453.2. [Q9DBT4-2]
UniGeneiMm.489765.

Genome annotation databases

EnsembliENSMUST00000064831; ENSMUSP00000065046; ENSMUSG00000095463. [Q9DBT4-1]
ENSMUST00000184973; ENSMUSP00000138944; ENSMUSG00000095463. [Q9DBT4-1]
ENSMUST00000185072; ENSMUSP00000139202; ENSMUSG00000022066. [Q9DBT4-1]
GeneIDi100862375.
67464.
KEGGimmu:100862375.
mmu:67464.
UCSCiuc007umi.2. mouse. [Q9DBT4-1]
uc007umk.2. mouse. [Q9DBT4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004761 mRNA. Translation: BAB23542.1.
BC006924 mRNA. Translation: AAH06924.1.
BC043134 mRNA. Translation: AAH43134.1.
CCDSiCCDS27240.1. [Q9DBT4-1]
RefSeqiNP_080450.1. NM_026174.3. [Q9DBT4-1]
XP_003688976.1. XM_003688928.2. [Q9DBT4-1]
XP_003688977.1. XM_003688929.4. [Q9DBT4-2]
XP_006519516.1. XM_006519453.2. [Q9DBT4-2]
UniGeneiMm.489765.

3D structure databases

ProteinModelPortaliQ9DBT4.
SMRiQ9DBT4. Positions 87-539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065046.

PTM databases

PhosphoSiteiQ9DBT4.

Proteomic databases

MaxQBiQ9DBT4.
PaxDbiQ9DBT4.
PRIDEiQ9DBT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064831; ENSMUSP00000065046; ENSMUSG00000095463. [Q9DBT4-1]
ENSMUST00000184973; ENSMUSP00000138944; ENSMUSG00000095463. [Q9DBT4-1]
ENSMUST00000185072; ENSMUSP00000139202; ENSMUSG00000022066. [Q9DBT4-1]
GeneIDi100862375.
67464.
KEGGimmu:100862375.
mmu:67464.
UCSCiuc007umi.2. mouse. [Q9DBT4-1]
uc007umk.2. mouse. [Q9DBT4-2]

Organism-specific databases

CTDi9583.
MGIiMGI:1914714. Entpd4.

Phylogenomic databases

eggNOGiKOG1386. Eukaryota.
COG5371. LUCA.
GeneTreeiENSGT00550000074435.
HOGENOMiHOG000090205.
HOVERGENiHBG006789.
InParanoidiQ9DBT4.
KOiK12305.
OMAiNSQFYGF.
OrthoDBiEOG793B78.
PhylomeDBiQ9DBT4.
TreeFamiTF354343.

Miscellaneous databases

ChiTaRSiEntpd4. mouse.
NextBioi324650.
PROiQ9DBT4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DBT4.
CleanExiMM_ENTPD4.
ExpressionAtlasiQ9DBT4. baseline.
GenevisibleiQ9DBT4. MM.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 3 hits.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Mammary gland.
  3. "First apyrase splice variants have different enzymatic properties."
    Biederbick A., Kosan C., Kunz J., Elsaesser H.-P.
    J. Biol. Chem. 275:19018-19024(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue.

Entry informationi

Entry nameiENTP4_MOUSE
AccessioniPrimary (citable) accession number: Q9DBT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: June 1, 2001
Last modified: February 17, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.