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Protein

A-kinase anchor protein 8

Gene

Akap8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri387 – 41125C2H2 AKAP95-type 1Add
BLAST
Zinc fingeri476 – 50126C2H2 AKAP95-type 2Add
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. double-stranded DNA binding Source: Ensembl
  3. poly(A) RNA binding Source: MGI
  4. zinc ion binding Source: Ensembl

GO - Biological processi

  1. mitotic chromosome condensation Source: MGI
Complete GO annotation...

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 8
Short name:
AKAP-8
Alternative name(s):
A-kinase anchor protein 95 kDa
Short name:
AKAP 95
Gene namesi
Name:Akap8
Synonyms:Akap95
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1928488. Akap8.

Subcellular locationi

Nucleus matrix 1 Publication
Note: Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis (By similarity).By similarity

GO - Cellular componenti

  1. condensed chromosome Source: MGI
  2. female pronucleus Source: MGI
  3. Golgi apparatus Source: Ensembl
  4. membrane Source: MGI
  5. mitochondrion Source: Ensembl
  6. nuclear chromatin Source: MGI
  7. nuclear matrix Source: UniProtKB
  8. nucleoplasm Source: MGI
  9. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

AKAP8 and FIGN double mutant mice die soon after birth due to cleft palate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 687687A-kinase anchor protein 8PRO_0000075382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei320 – 3201Phosphoserine1 Publication
Modified residuei325 – 3251Phosphoserine1 Publication
Modified residuei336 – 3361PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9DBR0.
PaxDbiQ9DBR0.
PRIDEiQ9DBR0.

PTM databases

PhosphoSiteiQ9DBR0.

Expressioni

Gene expression databases

BgeeiQ9DBR0.
CleanExiMM_AKAP8.
ExpressionAtlasiQ9DBR0. baseline and differential.
GenevestigatoriQ9DBR0.

Interactioni

Subunit structurei

Binds to dimeric RII-alpha regulatory subunit of PKA during mitosis (By similarity). Interacts (via C-terminus) with FIGN.By similarity1 Publication

Protein-protein interaction databases

BioGridi207953. 5 interactions.
IntActiQ9DBR0. 6 interactions.
MINTiMINT-4128362.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni568 – 58518RII-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi366 – 37510Nuclear localization signalSequence Analysis

Sequence similaritiesi

Belongs to the AKAP95 family.Curated
Contains 2 C2H2 AKAP95-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri387 – 41125C2H2 AKAP95-type 1Add
BLAST
Zinc fingeri476 – 50126C2H2 AKAP95-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47695.
GeneTreeiENSGT00530000063777.
HOGENOMiHOG000033876.
HOVERGENiHBG053198.
InParanoidiQ9DBR0.
KOiK16525.
OMAiHSVDHNH.
OrthoDBiEOG741Z1X.
PhylomeDBiQ9DBR0.
TreeFamiTF105407.

Family and domain databases

InterProiIPR007071. AKAP95.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERiPTHR12190. PTHR12190. 1 hit.
PfamiPF04988. AKAP95. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DBR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQGYGGYGA WSAGPANTQG TYGSGMTSWQ GYENYNYYNA QNTSVPAGTP
60 70 80 90 100
YSYGPASWEA TKTNDGGLAA GSPAMHVASF APEPCTDNSD SLIAKINQRL
110 120 130 140 150
DMLSKEGGRG GISSGGEGVQ DRDSSFRFQP YESYDARPCI PEHNPYRPGY
160 170 180 190 200
GYDYDFDLGT DRNGSFGGTF NDCRDPAPER GSLDGFLRGR GQGRFQDRSN
210 220 230 240 250
SSTFIRSDPF MPPSASEPLS TTWNELNYMG GRGLGGPSTS RPPPSLFSQS
260 270 280 290 300
MAPDYSMMGM QGVGGFGGTM PYGCGRSQTR IRDWPRRRGF ERFGPDNMGR
310 320 330 340 350
KRKQFPLYEE PDAKLARADS DGDLSENDDG AGDLRSGDEE FRGEDDLCDS
360 370 380 390 400
RKQRGEKEDE DEDVKKRREK QRRRDRMRDR AADRIQFACS VCKFRSFEDE
410 420 430 440 450
EIQKHLQSKF HKETLRFIST KLPDKTVEFL QEYIINRNKK IEKRRQELLE
460 470 480 490 500
KESPKPKPDP FKGIGQEHFF KKIEAAHCLA CDMLIPAQHQ LLQRHLHSVD
510 520 530 540 550
HNHNRRLAAE QFKKTSLHVA KSVLNNKHIV KMLEKYLKGE DPFVNETADL
560 570 580 590 600
ETEGDENVGE EKEETPEEVA AEVLAEVITA AVKAVEGEGE PAAAHSDVLT
610 620 630 640 650
EVEGPVDTAE ASSDPHTEKL LEEQTCEAAS ETRSIEDKTR GEAAEARNEA
660 670 680
AMPTADAGST LPVIAIPGIM EDELEQTGAE AKDIPTE
Length:687
Mass (Da):76,294
Last modified:June 1, 2001 - v1
Checksum:iBC1E320216A47E10
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti323 – 3231D → G in BAA84710 (Ref. 1) Curated
Sequence conflicti370 – 3701K → E in BAA84710 (Ref. 1) Curated
Sequence conflicti541 – 5411D → G in BAA84710 (Ref. 1) Curated
Sequence conflicti544 – 5441V → A in BAA84710 (Ref. 1) Curated
Sequence conflicti584 – 5841A → T in BAA84710 (Ref. 1) Curated
Sequence conflicti590 – 5901E → G in BAA84710 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028920 mRNA. Translation: BAA84710.1.
AK004801 mRNA. Translation: BAB23574.1.
AK089092 mRNA. Translation: BAC40746.1.
CCDSiCCDS37555.1.
RefSeqiNP_062748.2. NM_019774.4.
UniGeneiMm.328945.

Genome annotation databases

EnsembliENSMUST00000002699; ENSMUSP00000002699; ENSMUSG00000024045.
GeneIDi56399.
KEGGimmu:56399.
UCSCiuc008bwg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028920 mRNA. Translation: BAA84710.1.
AK004801 mRNA. Translation: BAB23574.1.
AK089092 mRNA. Translation: BAC40746.1.
CCDSiCCDS37555.1.
RefSeqiNP_062748.2. NM_019774.4.
UniGeneiMm.328945.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207953. 5 interactions.
IntActiQ9DBR0. 6 interactions.
MINTiMINT-4128362.

PTM databases

PhosphoSiteiQ9DBR0.

Proteomic databases

MaxQBiQ9DBR0.
PaxDbiQ9DBR0.
PRIDEiQ9DBR0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002699; ENSMUSP00000002699; ENSMUSG00000024045.
GeneIDi56399.
KEGGimmu:56399.
UCSCiuc008bwg.1. mouse.

Organism-specific databases

CTDi10270.
MGIiMGI:1928488. Akap8.

Phylogenomic databases

eggNOGiNOG47695.
GeneTreeiENSGT00530000063777.
HOGENOMiHOG000033876.
HOVERGENiHBG053198.
InParanoidiQ9DBR0.
KOiK16525.
OMAiHSVDHNH.
OrthoDBiEOG741Z1X.
PhylomeDBiQ9DBR0.
TreeFamiTF105407.

Miscellaneous databases

ChiTaRSiAkap8. mouse.
NextBioi312508.
PROiQ9DBR0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DBR0.
CleanExiMM_AKAP8.
ExpressionAtlasiQ9DBR0. baseline and differential.
GenevestigatoriQ9DBR0.

Family and domain databases

InterProiIPR007071. AKAP95.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERiPTHR12190. PTHR12190. 1 hit.
PfamiPF04988. AKAP95. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse AKAP95."
    Hattori A., Seki N., Hayashi A., Kozuma S., Muramatsu M., Miyajima N., Saito T.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Lung and Thymus.
  3. "Interaction between fidgetin and protein kinase A-anchoring protein AKAP95 is critical for palatogenesis in the mouse."
    Yang Y., Mahaffey C.L., Berube N., Frankel W.N.
    J. Biol. Chem. 281:22352-22359(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH FIGN, DISRUPTION PHENOTYPE.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-320 AND SER-325, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAKAP8_MOUSE
AccessioniPrimary (citable) accession number: Q9DBR0
Secondary accession number(s): Q9R0L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: March 4, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.