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Q9DBR0 (AKAP8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
A-kinase anchor protein 8

Short name=AKAP-8
Alternative name(s):
A-kinase anchor protein 95 kDa
Short name=AKAP 95
Gene names
Name:Akap8
Synonyms:Akap95
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length687 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) By similarity.

Subunit structure

Binds to dimeric RII-alpha regulatory subunit of PKA during mitosis By similarity. Interacts (via C-terminus) with FIGN. Ref.3

Subcellular location

Nucleus matrix. Note: Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis By similarity. Ref.3

Disruption phenotype

AKAP8 and FIGN double mutant mice die soon after birth due to cleft palate. Ref.3

Sequence similarities

Belongs to the AKAP95 family.

Contains 2 C2H2 AKAP95-type zinc fingers.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 687687A-kinase anchor protein 8
PRO_0000075382

Regions

Zinc finger387 – 41125C2H2 AKAP95-type 1
Zinc finger476 – 50126C2H2 AKAP95-type 2
Region568 – 58518RII-binding By similarity
Motif366 – 37510Nuclear localization signal Potential

Amino acid modifications

Modified residue3201Phosphoserine Ref.4
Modified residue3251Phosphoserine Ref.4
Modified residue3361Phosphoserine By similarity

Experimental info

Sequence conflict3231D → G in BAA84710. Ref.1
Sequence conflict3701K → E in BAA84710. Ref.1
Sequence conflict5411D → G in BAA84710. Ref.1
Sequence conflict5441V → A in BAA84710. Ref.1
Sequence conflict5841A → T in BAA84710. Ref.1
Sequence conflict5901E → G in BAA84710. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9DBR0 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: BC1E320216A47E10

FASTA68776,294
        10         20         30         40         50         60 
MEQGYGGYGA WSAGPANTQG TYGSGMTSWQ GYENYNYYNA QNTSVPAGTP YSYGPASWEA 

        70         80         90        100        110        120 
TKTNDGGLAA GSPAMHVASF APEPCTDNSD SLIAKINQRL DMLSKEGGRG GISSGGEGVQ 

       130        140        150        160        170        180 
DRDSSFRFQP YESYDARPCI PEHNPYRPGY GYDYDFDLGT DRNGSFGGTF NDCRDPAPER 

       190        200        210        220        230        240 
GSLDGFLRGR GQGRFQDRSN SSTFIRSDPF MPPSASEPLS TTWNELNYMG GRGLGGPSTS 

       250        260        270        280        290        300 
RPPPSLFSQS MAPDYSMMGM QGVGGFGGTM PYGCGRSQTR IRDWPRRRGF ERFGPDNMGR 

       310        320        330        340        350        360 
KRKQFPLYEE PDAKLARADS DGDLSENDDG AGDLRSGDEE FRGEDDLCDS RKQRGEKEDE 

       370        380        390        400        410        420 
DEDVKKRREK QRRRDRMRDR AADRIQFACS VCKFRSFEDE EIQKHLQSKF HKETLRFIST 

       430        440        450        460        470        480 
KLPDKTVEFL QEYIINRNKK IEKRRQELLE KESPKPKPDP FKGIGQEHFF KKIEAAHCLA 

       490        500        510        520        530        540 
CDMLIPAQHQ LLQRHLHSVD HNHNRRLAAE QFKKTSLHVA KSVLNNKHIV KMLEKYLKGE 

       550        560        570        580        590        600 
DPFVNETADL ETEGDENVGE EKEETPEEVA AEVLAEVITA AVKAVEGEGE PAAAHSDVLT 

       610        620        630        640        650        660 
EVEGPVDTAE ASSDPHTEKL LEEQTCEAAS ETRSIEDKTR GEAAEARNEA AMPTADAGST 

       670        680 
LPVIAIPGIM EDELEQTGAE AKDIPTE 

« Hide

References

« Hide 'large scale' references
[1]"Mouse AKAP95."
Hattori A., Seki N., Hayashi A., Kozuma S., Muramatsu M., Miyajima N., Saito T.
Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Lung and Thymus.
[3]"Interaction between fidgetin and protein kinase A-anchoring protein AKAP95 is critical for palatogenesis in the mouse."
Yang Y., Mahaffey C.L., Berube N., Frankel W.N.
J. Biol. Chem. 281:22352-22359(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH FIGN, DISRUPTION PHENOTYPE.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-320 AND SER-325, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB028920 mRNA. Translation: BAA84710.1.
AK004801 mRNA. Translation: BAB23574.1.
AK089092 mRNA. Translation: BAC40746.1.
CCDSCCDS37555.1.
RefSeqNP_062748.2. NM_019774.4.
UniGeneMm.328945.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207953. 5 interactions.
IntActQ9DBR0. 6 interactions.
MINTMINT-4128362.

PTM databases

PhosphoSiteQ9DBR0.

Proteomic databases

MaxQBQ9DBR0.
PaxDbQ9DBR0.
PRIDEQ9DBR0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002699; ENSMUSP00000002699; ENSMUSG00000024045.
GeneID56399.
KEGGmmu:56399.
UCSCuc008bwg.1. mouse.

Organism-specific databases

CTD10270.
MGIMGI:1928488. Akap8.

Phylogenomic databases

eggNOGNOG47695.
GeneTreeENSGT00530000063777.
HOGENOMHOG000033876.
HOVERGENHBG053198.
InParanoidQ9DBR0.
KOK16525.
OMAHSVDHNH.
OrthoDBEOG741Z1X.
PhylomeDBQ9DBR0.
TreeFamTF105407.

Gene expression databases

ArrayExpressQ9DBR0.
BgeeQ9DBR0.
CleanExMM_AKAP8.
GenevestigatorQ9DBR0.

Family and domain databases

InterProIPR007071. AKAP95.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERPTHR12190. PTHR12190. 1 hit.
PfamPF04988. AKAP95. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAKAP8. mouse.
NextBio312508.
PROQ9DBR0.
SOURCESearch...

Entry information

Entry nameAKAP8_MOUSE
AccessionPrimary (citable) accession number: Q9DBR0
Secondary accession number(s): Q9R0L8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot