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Q9DBR0

- AKAP8_MOUSE

UniProt

Q9DBR0 - AKAP8_MOUSE

Protein

A-kinase anchor protein 8

Gene

Akap8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri387 – 41125C2H2 AKAP95-type 1Add
    BLAST
    Zinc fingeri476 – 50126C2H2 AKAP95-type 2Add
    BLAST

    GO - Molecular functioni

    1. double-stranded DNA binding Source: Ensembl
    2. protein binding Source: UniProtKB
    3. zinc ion binding Source: Ensembl

    GO - Biological processi

    1. mitotic chromosome condensation Source: MGI

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    A-kinase anchor protein 8
    Short name:
    AKAP-8
    Alternative name(s):
    A-kinase anchor protein 95 kDa
    Short name:
    AKAP 95
    Gene namesi
    Name:Akap8
    Synonyms:Akap95
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1928488. Akap8.

    Subcellular locationi

    Nucleus matrix 1 Publication
    Note: Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis By similarity.By similarity

    GO - Cellular componenti

    1. condensed chromosome Source: MGI
    2. female pronucleus Source: MGI
    3. Golgi apparatus Source: Ensembl
    4. mitochondrion Source: Ensembl
    5. nuclear matrix Source: UniProtKB
    6. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    AKAP8 and FIGN double mutant mice die soon after birth due to cleft palate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 687687A-kinase anchor protein 8PRO_0000075382Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei320 – 3201Phosphoserine1 Publication
    Modified residuei325 – 3251Phosphoserine1 Publication
    Modified residuei336 – 3361PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9DBR0.
    PaxDbiQ9DBR0.
    PRIDEiQ9DBR0.

    PTM databases

    PhosphoSiteiQ9DBR0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9DBR0.
    BgeeiQ9DBR0.
    CleanExiMM_AKAP8.
    GenevestigatoriQ9DBR0.

    Interactioni

    Subunit structurei

    Binds to dimeric RII-alpha regulatory subunit of PKA during mitosis By similarity. Interacts (via C-terminus) with FIGN.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi207953. 5 interactions.
    IntActiQ9DBR0. 6 interactions.
    MINTiMINT-4128362.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni568 – 58518RII-bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi366 – 37510Nuclear localization signalSequence Analysis

    Sequence similaritiesi

    Belongs to the AKAP95 family.Curated
    Contains 2 C2H2 AKAP95-type zinc fingers.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri387 – 41125C2H2 AKAP95-type 1Add
    BLAST
    Zinc fingeri476 – 50126C2H2 AKAP95-type 2Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47695.
    GeneTreeiENSGT00530000063777.
    HOGENOMiHOG000033876.
    HOVERGENiHBG053198.
    InParanoidiQ9DBR0.
    KOiK16525.
    OMAiHSVDHNH.
    OrthoDBiEOG741Z1X.
    PhylomeDBiQ9DBR0.
    TreeFamiTF105407.

    Family and domain databases

    InterProiIPR007071. AKAP95.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PANTHERiPTHR12190. PTHR12190. 1 hit.
    PfamiPF04988. AKAP95. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9DBR0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQGYGGYGA WSAGPANTQG TYGSGMTSWQ GYENYNYYNA QNTSVPAGTP    50
    YSYGPASWEA TKTNDGGLAA GSPAMHVASF APEPCTDNSD SLIAKINQRL 100
    DMLSKEGGRG GISSGGEGVQ DRDSSFRFQP YESYDARPCI PEHNPYRPGY 150
    GYDYDFDLGT DRNGSFGGTF NDCRDPAPER GSLDGFLRGR GQGRFQDRSN 200
    SSTFIRSDPF MPPSASEPLS TTWNELNYMG GRGLGGPSTS RPPPSLFSQS 250
    MAPDYSMMGM QGVGGFGGTM PYGCGRSQTR IRDWPRRRGF ERFGPDNMGR 300
    KRKQFPLYEE PDAKLARADS DGDLSENDDG AGDLRSGDEE FRGEDDLCDS 350
    RKQRGEKEDE DEDVKKRREK QRRRDRMRDR AADRIQFACS VCKFRSFEDE 400
    EIQKHLQSKF HKETLRFIST KLPDKTVEFL QEYIINRNKK IEKRRQELLE 450
    KESPKPKPDP FKGIGQEHFF KKIEAAHCLA CDMLIPAQHQ LLQRHLHSVD 500
    HNHNRRLAAE QFKKTSLHVA KSVLNNKHIV KMLEKYLKGE DPFVNETADL 550
    ETEGDENVGE EKEETPEEVA AEVLAEVITA AVKAVEGEGE PAAAHSDVLT 600
    EVEGPVDTAE ASSDPHTEKL LEEQTCEAAS ETRSIEDKTR GEAAEARNEA 650
    AMPTADAGST LPVIAIPGIM EDELEQTGAE AKDIPTE 687
    Length:687
    Mass (Da):76,294
    Last modified:June 1, 2001 - v1
    Checksum:iBC1E320216A47E10
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti323 – 3231D → G in BAA84710. 1 PublicationCurated
    Sequence conflicti370 – 3701K → E in BAA84710. 1 PublicationCurated
    Sequence conflicti541 – 5411D → G in BAA84710. 1 PublicationCurated
    Sequence conflicti544 – 5441V → A in BAA84710. 1 PublicationCurated
    Sequence conflicti584 – 5841A → T in BAA84710. 1 PublicationCurated
    Sequence conflicti590 – 5901E → G in BAA84710. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028920 mRNA. Translation: BAA84710.1.
    AK004801 mRNA. Translation: BAB23574.1.
    AK089092 mRNA. Translation: BAC40746.1.
    CCDSiCCDS37555.1.
    RefSeqiNP_062748.2. NM_019774.4.
    UniGeneiMm.328945.

    Genome annotation databases

    EnsembliENSMUST00000002699; ENSMUSP00000002699; ENSMUSG00000024045.
    GeneIDi56399.
    KEGGimmu:56399.
    UCSCiuc008bwg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB028920 mRNA. Translation: BAA84710.1 .
    AK004801 mRNA. Translation: BAB23574.1 .
    AK089092 mRNA. Translation: BAC40746.1 .
    CCDSi CCDS37555.1.
    RefSeqi NP_062748.2. NM_019774.4.
    UniGenei Mm.328945.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207953. 5 interactions.
    IntActi Q9DBR0. 6 interactions.
    MINTi MINT-4128362.

    PTM databases

    PhosphoSitei Q9DBR0.

    Proteomic databases

    MaxQBi Q9DBR0.
    PaxDbi Q9DBR0.
    PRIDEi Q9DBR0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002699 ; ENSMUSP00000002699 ; ENSMUSG00000024045 .
    GeneIDi 56399.
    KEGGi mmu:56399.
    UCSCi uc008bwg.1. mouse.

    Organism-specific databases

    CTDi 10270.
    MGIi MGI:1928488. Akap8.

    Phylogenomic databases

    eggNOGi NOG47695.
    GeneTreei ENSGT00530000063777.
    HOGENOMi HOG000033876.
    HOVERGENi HBG053198.
    InParanoidi Q9DBR0.
    KOi K16525.
    OMAi HSVDHNH.
    OrthoDBi EOG741Z1X.
    PhylomeDBi Q9DBR0.
    TreeFami TF105407.

    Miscellaneous databases

    ChiTaRSi AKAP8. mouse.
    NextBioi 312508.
    PROi Q9DBR0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9DBR0.
    Bgeei Q9DBR0.
    CleanExi MM_AKAP8.
    Genevestigatori Q9DBR0.

    Family and domain databases

    InterProi IPR007071. AKAP95.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    PANTHERi PTHR12190. PTHR12190. 1 hit.
    Pfami PF04988. AKAP95. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse AKAP95."
      Hattori A., Seki N., Hayashi A., Kozuma S., Muramatsu M., Miyajima N., Saito T.
      Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Lung and Thymus.
    3. "Interaction between fidgetin and protein kinase A-anchoring protein AKAP95 is critical for palatogenesis in the mouse."
      Yang Y., Mahaffey C.L., Berube N., Frankel W.N.
      J. Biol. Chem. 281:22352-22359(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH FIGN, DISRUPTION PHENOTYPE.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-320 AND SER-325, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAKAP8_MOUSE
    AccessioniPrimary (citable) accession number: Q9DBR0
    Secondary accession number(s): Q9R0L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3