Q9DBP0 (NPT2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium-dependent phosphate transport protein 2B
Short name=Sodium-phosphate transport protein 2B

Alternative name(s):
Na(+)-dependent phosphate cotransporter 2B
Sodium/phosphate cotransporter 2B
Short name=Na(+)/Pi cotransporter 2B
Short name=NaPi-2b
Solute carrier family 34 member 2

Gene names

Name:	Slc34a2
Synonyms:	Npt2b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	697 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in actively transporting phosphate into cells via Na+ cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Highly abundant in the ileum of small intestine, whereas it is almost absent in the duodenum and in the jejunum. Ref.4
Induction	Up-regulated in the entire small intestine by low-phosphate diet. Up-regulated by metabolic acidosis. Ref.4 Ref.5
Sequence similarities	Belongs to the SLC34A transporter family.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Symport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from electronic annotation. Source: Compara cellular phosphate ion homeostasis Inferred from electronic annotation. Source: Compara in utero embryonic development Inferred from mutant phenotype PubMed 19233126. Source: MGI response to estradiol stimulus Inferred from electronic annotation. Source: Compara response to fructose stimulus Inferred from electronic annotation. Source: Compara
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 11706048PubMed 12121891PubMed 12773415PubMed 17574207Ref.1. Source: MGI brush border Inferred from direct assay PubMed 12773415. Source: MGI brush border membrane Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	phosphate ion binding Inferred from electronic annotation. Source: Compara sodium ion binding Inferred from electronic annotation. Source: Compara sodium-dependent phosphate transmembrane transporter activity Inferred from direct assay Ref.1. Source: MGI sodium:phosphate symporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 697	697	Sodium-dependent phosphate transport protein 2B		PRO_0000068614
Regions
	Topological domain	1 – 91	91	Cytoplasmic Potential
	Transmembrane	92 – 112	21	Helical; Name=M1; Potential
	Topological domain	113 – 136	24	Extracellular Potential
	Transmembrane	137 – 157	21	Helical; Name=M2; Potential
	Topological domain	158 – 213	56	Cytoplasmic Potential
	Transmembrane	214 – 234	21	Helical; Name=M3; Potential
	Topological domain	235 – 363	129	Extracellular Potential
	Transmembrane	364 – 384	21	Helical; Name=M4; Potential
	Topological domain	385 – 408	24	Cytoplasmic Potential
	Transmembrane	409 – 429	21	Helical; Name=M5; Potential
	Topological domain	430 – 486	57	Extracellular Potential
	Transmembrane	487 – 507	21	Helical; Name=M6; Potential
	Topological domain	508 – 526	19	Cytoplasmic Potential
	Transmembrane	527 – 547	21	Helical; Name=M7; Potential
	Topological domain	548 – 551	4	Extracellular Potential
	Transmembrane	552 – 572	21	Helical; Name=M8; Potential
	Topological domain	573 – 696	124	Cytoplasmic Potential
	Compositional bias	465 – 468	4	Poly-Thr
	Compositional bias	535 – 538	4	Poly-Phe
	Compositional bias	564 – 572	9	Poly-Leu
	Compositional bias	613 – 644	32	Cys-rich
	Compositional bias	649 – 655	7	Poly-Glu
Amino acid modifications
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
	Glycosylation	308	1	N-linked (GlcNAc...) Potential
	Glycosylation	321	1	N-linked (GlcNAc...) Potential
	Glycosylation	356	1	N-linked (GlcNAc...) Potential
	Disulfide bond	303 ↔ 350		By similarity
Experimental info
	Sequence conflict	81	1	K → R in AAC80007. Ref.1
	Sequence conflict	155	1	V → L in AAC80007. Ref.1
	Sequence conflict	622	1	Missing Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9DBP0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 2A7B9384857EF16F	FASTA	697	76,245
	10 20 30 40 50 60 MAPWPELENA QPNPGKFIEG ASGPQSSIPA KDKEASKTND NGTPVAKTEL LPSYSALVLI 70 80 90 100 110 120 EEHPEGTDPW DLPELQDTGI KWSERDTKGK TLCIFQGVGK FILLLGFLYL FVCSLDVLSS 130 140 150 160 170 180 AFQLVGGKVA GQFFSNNSIM SNPVAGLVIG VLVTVMVQSS STSSSIIVSM VASSLLTVRA 190 200 210 220 230 240 AIPIIMGANI GTSITNTIVA LMQAGDRNEF RRAFAGATVH DFFNWLSVFV LLPLEAATHY 250 260 270 280 290 300 LEILTNLVLE TFKFQNGEDA PDILKVITDP FTKLIIQLDK KVIQQIAMGD SAAQNKSLIK 310 320 330 340 350 360 IWCKSITNVT EMNVTVPSTD NCTSPSYCWT DGIQTWTIQN VTQKENIAKC QHIFVNFSLP 370 380 390 400 410 420 DLAVGIILLT VSLVVLCGCL IMIVKLLGSV LRGQVATVIK KTLNTDFPFP FAWLTGYLAI 430 440 450 460 470 480 LVGAGMTFIV QSSSVFTSAM TPLIGIGVIS IERAYPLTLG SNIGTTTTAI LAALASPGNT 490 500 510 520 530 540 LRSSLQIALC HFFFNISGIL LWYPIPFTRL PIRLAKGLGN ISAKYRWFAV FYLIFFFFVT 550 560 570 580 590 600 PLTVFGLSLA GWPVLVGVGV PIILLLLLVL CLRMLQFRCP RILPLKLRDW NFLPLWMHSL 610 620 630 640 650 660 KPWDNVISLA TTCFQRRCCC CCRVCCRVCC MVCGCKCCRC SKCCRDQGEE EEEKEQDIPV 670 680 690 KASGAFDNAA MSKECQDEGK GQVEVLSMKA LSNTTVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a murine type II sodium-phosphate cotransporter expressed in mammalian small intestine." Hilfiker H., Hattenhauer O., Traebert M., Forster I.C., Murer H., Biber J. Proc. Natl. Acad. Sci. U.S.A. 95:14564-14569(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NMRI. Tissue: Small intestine.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[4]	"Regulation of intestinal phosphate transport. I. Segmental expression and adaptation to low-P(i) diet of the type IIb Na(+)-P(i) cotransporter in mouse small intestine." Radanovic T., Wagner C.A., Murer H., Biber J. Am. J. Physiol. 288:G496-G500(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, TISSUE SPECIFICITY. Strain: NMRI.
[5]	"Regulation of intestinal phosphate transport. II. Metabolic acidosis stimulates Na(+)-dependent phosphate absorption and expression of the Na(+)-P(i) cotransporter NaPi-IIb in small intestine." Stauber A., Radanovic T., Stange G., Murer H., Wagner C.A., Biber J. Am. J. Physiol. 288:G501-G506(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF081499 mRNA. Translation: AAC80007.1. AK004832 mRNA. Translation: BAB23600.1. AK145599 mRNA. Translation: BAE26533.1. BC096369 mRNA. Translation: AAH96369.1.
IPI	IPI00321717.
RefSeq	NP_035532.2. NM_011402.3.
UniGene	Mm.284891.
3D structure databases
ProteinModelPortal	Q9DBP0.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000092380.
PTM databases
PhosphoSite	Q9DBP0.
Proteomic databases
PaxDb	Q9DBP0.
PRIDE	Q9DBP0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000094787; ENSMUSP00000092380; ENSMUSG00000029188.
GeneID	20531.
KEGG	mmu:20531.
UCSC	uc008xla.2. mouse.
Organism-specific databases
CTD	10568.
MGI	MGI:1342284. Slc34a2.
Phylogenomic databases
eggNOG	COG1283.
GeneTree	ENSGT00390000005032.
HOGENOM	HOG000006550.
HOVERGEN	HBG006527.
InParanoid	Q9DBP0.
KO	K14683.
OMA	KCCEDLE.
OrthoDB	EOG473PRH.
Gene expression databases
ArrayExpress	Q9DBP0.
Bgee	Q9DBP0.
Genevestigator	Q9DBP0.
GermOnline	ENSMUSG00000029188. Mus musculus.
Family and domain databases
InterPro	IPR003841. Na/Pi_transpt. [Graphical view]
Pfam	PF02690. Na_Pi_cotrans. 2 hits. [Graphical view]
TIGRFAMs	TIGR01013. 2a58. 1 hit.
ProtoNet	Search...
Other
NextBio	298787.
SOURCE	Search...

Entry information

Entry name

NPT2B_MOUSE

Accession

Primary (citable) accession number: Q9DBP0
Secondary accession number(s): Q9Z290

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program