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Q9DBP0 (NPT2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium-dependent phosphate transport protein 2B

Short name=Sodium-phosphate transport protein 2B
Alternative name(s):
Na(+)-dependent phosphate cotransporter 2B
Sodium/phosphate cotransporter 2B
Short name=Na(+)/Pi cotransporter 2B
Short name=NaPi-2b
Solute carrier family 34 member 2
Gene names
Name:Slc34a2
Synonyms:Npt2b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length697 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in actively transporting phosphate into cells via Na+ cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Highly abundant in the ileum of small intestine, whereas it is almost absent in the duodenum and in the jejunum. Ref.4

Induction

Up-regulated in the entire small intestine by low-phosphate diet. Up-regulated by metabolic acidosis. Ref.4 Ref.5

Sequence similarities

Belongs to the SLC34A transporter family.

Ontologies

Keywords
   Biological processIon transport
Sodium transport
Symport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandSodium
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from electronic annotation. Source: Ensembl

cellular phosphate ion homeostasis

Inferred from electronic annotation. Source: Ensembl

in utero embryonic development

Inferred from mutant phenotype PubMed 19233126. Source: MGI

phosphate ion transport

Inferred from direct assay Ref.1. Source: MGI

response to estradiol

Inferred from electronic annotation. Source: Ensembl

response to fructose

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentapical plasma membrane

Inferred from direct assay PubMed 11706048PubMed 12121891PubMed 12773415PubMed 17574207Ref.1. Source: MGI

brush border

Inferred from direct assay PubMed 12773415. Source: MGI

brush border membrane

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

microvillus membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionphosphate ion binding

Inferred from electronic annotation. Source: Ensembl

sodium ion binding

Inferred from electronic annotation. Source: Ensembl

sodium-dependent phosphate transmembrane transporter activity

Inferred from direct assay Ref.1. Source: MGI

sodium:phosphate symporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 697697Sodium-dependent phosphate transport protein 2B
PRO_0000068614

Regions

Topological domain1 – 9191Cytoplasmic Potential
Transmembrane92 – 11221Helical; Name=M1; Potential
Topological domain113 – 13624Extracellular Potential
Transmembrane137 – 15721Helical; Name=M2; Potential
Topological domain158 – 21356Cytoplasmic Potential
Transmembrane214 – 23421Helical; Name=M3; Potential
Topological domain235 – 363129Extracellular Potential
Transmembrane364 – 38421Helical; Name=M4; Potential
Topological domain385 – 40824Cytoplasmic Potential
Transmembrane409 – 42921Helical; Name=M5; Potential
Topological domain430 – 48657Extracellular Potential
Transmembrane487 – 50721Helical; Name=M6; Potential
Topological domain508 – 52619Cytoplasmic Potential
Transmembrane527 – 54721Helical; Name=M7; Potential
Topological domain548 – 5514Extracellular Potential
Transmembrane552 – 57221Helical; Name=M8; Potential
Topological domain573 – 696124Cytoplasmic Potential
Compositional bias465 – 4684Poly-Thr
Compositional bias535 – 5384Poly-Phe
Compositional bias564 – 5729Poly-Leu
Compositional bias613 – 64432Cys-rich
Compositional bias649 – 6557Poly-Glu

Amino acid modifications

Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation3081N-linked (GlcNAc...) Potential
Glycosylation3211N-linked (GlcNAc...) Potential
Glycosylation3561N-linked (GlcNAc...) Potential
Disulfide bond303 ↔ 350 By similarity

Experimental info

Sequence conflict811K → R in AAC80007. Ref.1
Sequence conflict1551V → L in AAC80007. Ref.1
Sequence conflict6221Missing Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9DBP0 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 2A7B9384857EF16F

FASTA69776,245
        10         20         30         40         50         60 
MAPWPELENA QPNPGKFIEG ASGPQSSIPA KDKEASKTND NGTPVAKTEL LPSYSALVLI 

        70         80         90        100        110        120 
EEHPEGTDPW DLPELQDTGI KWSERDTKGK TLCIFQGVGK FILLLGFLYL FVCSLDVLSS 

       130        140        150        160        170        180 
AFQLVGGKVA GQFFSNNSIM SNPVAGLVIG VLVTVMVQSS STSSSIIVSM VASSLLTVRA 

       190        200        210        220        230        240 
AIPIIMGANI GTSITNTIVA LMQAGDRNEF RRAFAGATVH DFFNWLSVFV LLPLEAATHY 

       250        260        270        280        290        300 
LEILTNLVLE TFKFQNGEDA PDILKVITDP FTKLIIQLDK KVIQQIAMGD SAAQNKSLIK 

       310        320        330        340        350        360 
IWCKSITNVT EMNVTVPSTD NCTSPSYCWT DGIQTWTIQN VTQKENIAKC QHIFVNFSLP 

       370        380        390        400        410        420 
DLAVGIILLT VSLVVLCGCL IMIVKLLGSV LRGQVATVIK KTLNTDFPFP FAWLTGYLAI 

       430        440        450        460        470        480 
LVGAGMTFIV QSSSVFTSAM TPLIGIGVIS IERAYPLTLG SNIGTTTTAI LAALASPGNT 

       490        500        510        520        530        540 
LRSSLQIALC HFFFNISGIL LWYPIPFTRL PIRLAKGLGN ISAKYRWFAV FYLIFFFFVT 

       550        560        570        580        590        600 
PLTVFGLSLA GWPVLVGVGV PIILLLLLVL CLRMLQFRCP RILPLKLRDW NFLPLWMHSL 

       610        620        630        640        650        660 
KPWDNVISLA TTCFQRRCCC CCRVCCRVCC MVCGCKCCRC SKCCRDQGEE EEEKEQDIPV 

       670        680        690 
KASGAFDNAA MSKECQDEGK GQVEVLSMKA LSNTTVF 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a murine type II sodium-phosphate cotransporter expressed in mammalian small intestine."
Hilfiker H., Hattenhauer O., Traebert M., Forster I.C., Murer H., Biber J.
Proc. Natl. Acad. Sci. U.S.A. 95:14564-14569(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: NMRI.
Tissue: Small intestine.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Lung.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Regulation of intestinal phosphate transport. I. Segmental expression and adaptation to low-P(i) diet of the type IIb Na(+)-P(i) cotransporter in mouse small intestine."
Radanovic T., Wagner C.A., Murer H., Biber J.
Am. J. Physiol. 288:G496-G500(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, TISSUE SPECIFICITY.
Strain: NMRI.
[5]"Regulation of intestinal phosphate transport. II. Metabolic acidosis stimulates Na(+)-dependent phosphate absorption and expression of the Na(+)-P(i) cotransporter NaPi-IIb in small intestine."
Stauber A., Radanovic T., Stange G., Murer H., Wagner C.A., Biber J.
Am. J. Physiol. 288:G501-G506(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF081499 mRNA. Translation: AAC80007.1.
AK004832 mRNA. Translation: BAB23600.1.
AK145599 mRNA. Translation: BAE26533.1.
BC096369 mRNA. Translation: AAH96369.1.
RefSeqNP_035532.2. NM_011402.3.
UniGeneMm.284891.

3D structure databases

ProteinModelPortalQ9DBP0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4998123.
STRING10090.ENSMUSP00000092380.

PTM databases

PhosphoSiteQ9DBP0.

Proteomic databases

PaxDbQ9DBP0.
PRIDEQ9DBP0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000094787; ENSMUSP00000092380; ENSMUSG00000029188.
GeneID20531.
KEGGmmu:20531.
UCSCuc008xla.2. mouse.

Organism-specific databases

CTD10568.
MGIMGI:1342284. Slc34a2.

Phylogenomic databases

eggNOGCOG1283.
GeneTreeENSGT00390000005032.
HOGENOMHOG000006550.
HOVERGENHBG006527.
InParanoidQ9DBP0.
KOK14683.
OMAYSHESTD.
OrthoDBEOG72ZCDP.
PhylomeDBQ9DBP0.
TreeFamTF313981.

Gene expression databases

BgeeQ9DBP0.
GenevestigatorQ9DBP0.

Family and domain databases

InterProIPR003841. Na/Pi_transpt.
[Graphical view]
PfamPF02690. Na_Pi_cotrans. 2 hits.
[Graphical view]
TIGRFAMsTIGR01013. 2a58. 1 hit.
ProtoNetSearch...

Other

NextBio298787.
PROQ9DBP0.
SOURCESearch...

Entry information

Entry nameNPT2B_MOUSE
AccessionPrimary (citable) accession number: Q9DBP0
Secondary accession number(s): Q9Z290
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot