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Q9DBN9 (DDX59_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent RNA helicase DDX59

EC=3.6.4.13
Alternative name(s):
DEAD box protein 59
Gene names
Name:Ddx59
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length619 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + H2O = ADP + phosphate.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Developmental stage

AT 11.5 dpc, expressed in the developing snout region, eye and limb buds. At 13.5 dpc, highly enriched in the lips, palatal shelves (secondary palate), and developing limb buds. Ref.3

Domain

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similarities

Belongs to the DEAD box helicase family. DDX59 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 HIT-type zinc finger.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainZinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
   Molecular functionHelicase
Hydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9DBN9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9DBN9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     269-292: DKTPSALILTPTRELAIQIERQAK → VIYQSDMSSSLKQTRDLPSFIVCN
     293-619: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 619619Probable ATP-dependent RNA helicase DDX59
PRO_0000282714

Regions

Domain234 – 405172Helicase ATP-binding
Domain416 – 579164Helicase C-terminal
Zinc finger104 – 13330HIT-type
Nucleotide binding247 – 2548ATP By similarity
Motif203 – 23129Q motif
Motif353 – 3564DEAD box

Amino acid modifications

Modified residue641Phosphoserine By similarity
Modified residue1601Phosphoserine By similarity

Natural variations

Alternative sequence269 – 29224DKTPS…ERQAK → VIYQSDMSSSLKQTRDLPSF IVCN in isoform 2.
VSP_026429
Alternative sequence293 – 619327Missing in isoform 2.
VSP_026430

Experimental info

Sequence conflict2081C → S in BAB28694. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: E4FE6E723D125697

FASTA61968,234
        10         20         30         40         50         60 
MFVPRSLKIK RSSNDDLKSG EAKKSKPEAG GLQVEGDRDT PVHTSVTEEA VTADKPGHAS 

        70         80         90        100        110        120 
STNSPSCQLA EVSSTGPDEG VKDSHPSEEP VKSFSKTQRW PEPGEPVCVV CGRYGEYICD 

       130        140        150        160        170        180 
KTDEDVCSLE CKAKHLLQVK EGEGSLRPSS PQRVAAEPES PLDAFYVYKE HPFIVTLKED 

       190        200        210        220        230        240 
QIETLKQQLG ISVQGQDVAR PIIDFEHCGF PETLNQNLKK SGYEVPTPIQ MQMIPVGLLG 

       250        260        270        280        290        300 
RDILASADTG SGKTAAFLLP VIIRAFSEDK TPSALILTPT RELAIQIERQ AKELMSGLPR 

       310        320        330        340        350        360 
MKTVLLVGGL PLPPQLYRLR QHVKVIIATP GRLLDIIKQS SVSLSGIKIV VVDEADTMLK 

       370        380        390        400        410        420 
MGFQQQVLDV LEHTPGDCQT ILVSATIPDS IEQLTDQLLH NPVRIITGDK NLPCASVRQI 

       430        440        450        460        470        480 
ILWVEDPAKK KKLFEILNDQ KLFKPPVLVF VDCKLGADLL SEAVQKITGL NSTSIHSEKS 

       490        500        510        520        530        540 
QVERRDILKG LLEGDYEVVV STGVLGRGLD LVNVKLVVNF DMPSSMDEYV HQVGRVGRLG 

       550        560        570        580        590        600 
QNGTAITFIN NNSKRLFWDV AKRVKPTGSI LPPQLLNSPY LHEQKRKEQQ KDRQTQNSLV 

       610 
TGANLMDIIR KHEKSSSQK 

« Hide

Isoform 2 [UniParc].

Checksum: B178BF6B8DCA3B90
Show »

FASTA29231,851

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Head and Lung.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[3]"Mutations in DDX59 implicate RNA helicase in the pathogenesis of orofaciodigital syndrome."
Shamseldin H.E., Rajab A., Alhashem A., Shaheen R., Al-Shidi T., Alamro R., Al Harassi S., Alkuraya F.S.
Am. J. Hum. Genet. 93:555-560(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK004833 mRNA. Translation: BAB23601.1.
AK013179 mRNA. Translation: BAB28694.1.
AK076462 mRNA. Translation: BAC36354.1.
BC023840 mRNA. Translation: AAH23840.1.
CCDSCCDS15326.1. [Q9DBN9-1]
RefSeqNP_080776.1. NM_026500.3. [Q9DBN9-1]
UniGeneMm.385462.
Mm.486546.

3D structure databases

ProteinModelPortalQ9DBN9.
SMRQ9DBN9. Positions 96-143, 168-583.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9DBN9.

Proteomic databases

MaxQBQ9DBN9.
PRIDEQ9DBN9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027655; ENSMUSP00000027655; ENSMUSG00000026404. [Q9DBN9-1]
GeneID67997.
KEGGmmu:67997.
UCSCuc007cut.1. mouse. [Q9DBN9-2]
uc007cuu.2. mouse. [Q9DBN9-1]

Organism-specific databases

CTD83479.
MGIMGI:1915247. Ddx59.

Phylogenomic databases

eggNOGCOG0513.
GeneTreeENSGT00740000115519.
HOGENOMHOG000006599.
HOVERGENHBG106120.
InParanoidQ9DBN9.
OMAEHPFILN.
OrthoDBEOG7B8S3D.
PhylomeDBQ9DBN9.
TreeFamTF330866.

Gene expression databases

BgeeQ9DBN9.
CleanExMM_DDX59.
GenevestigatorQ9DBN9.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR007529. Znf_HIT.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF04438. zf-HIT. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDDX59. mouse.
NextBio326168.
PROQ9DBN9.
SOURCESearch...

Entry information

Entry nameDDX59_MOUSE
AccessionPrimary (citable) accession number: Q9DBN9
Secondary accession number(s): Q8C667, Q9CSD1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot