Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lon protease homolog 2, peroxisomal

Gene

Lonp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import. May indirectly regulate peroxisomal fatty acid beta-oxidation through degradation of the self-processed forms of TYSND1.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei743 – 7431UniRule annotation
Active sitei786 – 7861UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi375 – 3828ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lon protease homolog 2, peroxisomalUniRule annotation (EC:3.4.21.-UniRule annotation)
Alternative name(s):
Lon protease-like protein 2UniRule annotation
Short name:
Lon protease 2UniRule annotation
Peroxisomal Lon proteaseUniRule annotation
Gene namesi
Name:Lonp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1914137. Lonp2.

Subcellular locationi

  • Peroxisome matrix UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: MGI
  • membrane Source: MGI
  • nucleus Source: MGI
  • peroxisomal matrix Source: HGNC
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 852851Lon protease homolog 2, peroxisomalPRO_0000287641Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9DBN5.
MaxQBiQ9DBN5.
PaxDbiQ9DBN5.
PeptideAtlasiQ9DBN5.
PRIDEiQ9DBN5.

PTM databases

iPTMnetiQ9DBN5.
PhosphoSiteiQ9DBN5.
SwissPalmiQ9DBN5.

Expressioni

Gene expression databases

BgeeiQ9DBN5.
CleanExiMM_LONP2.
ExpressionAtlasiQ9DBN5. baseline and differential.
GenevisibleiQ9DBN5. MM.

Interactioni

Subunit structurei

Interacts with PEX5. Interacts with TYSND1.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211789. 1 interaction.
IntActiQ9DBN5. 1 interaction.
MINTiMINT-4128330.
STRINGi10090.ENSMUSP00000034141.

Structurei

3D structure databases

ProteinModelPortaliQ9DBN5.
SMRiQ9DBN5. Positions 269-835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 220208Lon N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini651 – 837187Lon proteolyticPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi850 – 8523Microbody targeting signalUniRule annotation

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon N-terminal domain.PROSITE-ProRule annotation
Contains 1 Lon proteolytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2004. Eukaryota.
COG0466. LUCA.
GeneTreeiENSGT00530000063553.
HOGENOMiHOG000261408.
HOVERGENiHBG000798.
InParanoidiQ9DBN5.
KOiK01338.
OMAiFVYGLAW.
OrthoDBiEOG77T13V.
PhylomeDBiQ9DBN5.
TreeFamiTF317215.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03121. lonp2_euk.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027501. Lonp2_euk.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DBN5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSVSPIQIP SRLPLLLTHE SVLLPGSTMR TSVDTARNLQ LVRSRLLKGT
60 70 80 90 100
SLQSTILGVI PNTPDPASDT QDLPPLHRIG TAALAVQVVG SNWPKPHYTL
110 120 130 140 150
LITGLCRFQI VQVLKEKPYP VAEVEQLDRL EEFPNICKSR EELGELSEQF
160 170 180 190 200
YRYAVQLVEM LDMSVPAVAK LRRLLDNLPR EALPDILTSI IRTSNKEKLQ
210 220 230 240 250
ILDAVSLEDR FKMTIPLLVR QIEGLKLLQK TRKPKQDDDK RVIAIRPIRR
260 270 280 290 300
IPHIPGTLED EEEEEDNDDI VMLEKKIRTS SMPEQAHKVC VKEIKRLKKM
310 320 330 340 350
PQSMPEYALT RNYLELMVEL PWNKSTTDRL DIRAARILLD NDHYAMEKLK
360 370 380 390 400
RRVLEYLAVR QLKNNLKGPI LCFVGPPGVG KTSVGRSVAK TLGREFHRIA
410 420 430 440 450
LGGVCDQSDI RGHRRTYVGS MPGRIINGLK TVGVNNPVFL LDEVDKLGKS
460 470 480 490 500
LQGDPAAALL EVLDPEQNHN FTDHYLNVAF DLSQVLFIAT ANTTATIPPA
510 520 530 540 550
LLDRMEIIQV PGYTQEEKIE IAHRHLIPKQ LEQHGLTPQQ IQIPQHTTLA
560 570 580 590 600
IITRYTREAG VRSLDRKFGA ICRAVAVKVA EGQHKEAKLD RSDVADGEGC
610 620 630 640 650
KEHVLEDAKP ESISDTADLA LPPEMPILID SHALKDILGP PLYELEVSER
660 670 680 690 700
LSQPGVAIGL AWTPLGGKIM FVEASRMDGE GQLTLTGQLG DVMKESAHLA
710 720 730 740 750
ISWLRSNAKK YHLTNAFGSF DLLDNTDIHL HFPAGAVTKD GPSAGVTIVT
760 770 780 790 800
CLASLFSGRL VRSDVAMTGE ITLRGLVLPV GGIKDKVLAA HRAGLKQIII
810 820 830 840 850
PQRNEKDLEE IPSNVRQDLS FVTASCLDEV LNAAFDGGFP VKTRPGLIDS

KL
Length:852
Mass (Da):94,526
Last modified:June 1, 2001 - v1
Checksum:i890BD7E654D04DC7
GO
Isoform 2 (identifier: Q9DBN5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     648-659: SERLSQPGVAIG → MILISMFTLVLA
     660-852: Missing.

Note: No experimental confirmation available.
Show »
Length:659
Mass (Da):73,989
Checksum:iEC4B4E725B6D87F3
GO
Isoform 3 (identifier: Q9DBN5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-319: Missing.

Note: No experimental confirmation available.
Show »
Length:710
Mass (Da):77,891
Checksum:i7A67CC6266AFB55E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871Q → P in BAE30373 (PubMed:16141072).Curated
Sequence conflicti348 – 3481K → E in BAE40624 (PubMed:16141072).Curated
Sequence conflicti348 – 3481K → E in BAE40762 (PubMed:16141072).Curated
Sequence conflicti740 – 7401D → V in BAE30373 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei178 – 319142Missing in isoform 3. 1 PublicationVSP_025576Add
BLAST
Alternative sequencei648 – 65912SERLS…GVAIG → MILISMFTLVLA in isoform 2. 1 PublicationVSP_025577Add
BLAST
Alternative sequencei660 – 852193Missing in isoform 2. 1 PublicationVSP_025578Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004843 mRNA. Translation: BAB23609.1.
AK050231 mRNA. Translation: BAC34137.1.
AK151103 mRNA. Translation: BAE30113.1.
AK151406 mRNA. Translation: BAE30373.1.
AK168791 mRNA. Translation: BAE40624.1.
AK168628 mRNA. Translation: BAE40488.1.
AK168956 mRNA. Translation: BAE40762.1.
AK169656 mRNA. Translation: BAE41280.1.
BC049090 mRNA. Translation: AAH49090.1.
CCDSiCCDS22503.1. [Q9DBN5-1]
RefSeqiNP_001162063.1. NM_001168591.1.
NP_080103.1. NM_025827.3. [Q9DBN5-1]
UniGeneiMm.324550.

Genome annotation databases

EnsembliENSMUST00000034141; ENSMUSP00000034141; ENSMUSG00000047866. [Q9DBN5-1]
ENSMUST00000122188; ENSMUSP00000113834; ENSMUSG00000047866. [Q9DBN5-3]
ENSMUST00000155433; ENSMUSP00000118737; ENSMUSG00000047866. [Q9DBN5-2]
GeneIDi66887.
KEGGimmu:66887.
UCSCiuc009mqm.3. mouse. [Q9DBN5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004843 mRNA. Translation: BAB23609.1.
AK050231 mRNA. Translation: BAC34137.1.
AK151103 mRNA. Translation: BAE30113.1.
AK151406 mRNA. Translation: BAE30373.1.
AK168791 mRNA. Translation: BAE40624.1.
AK168628 mRNA. Translation: BAE40488.1.
AK168956 mRNA. Translation: BAE40762.1.
AK169656 mRNA. Translation: BAE41280.1.
BC049090 mRNA. Translation: AAH49090.1.
CCDSiCCDS22503.1. [Q9DBN5-1]
RefSeqiNP_001162063.1. NM_001168591.1.
NP_080103.1. NM_025827.3. [Q9DBN5-1]
UniGeneiMm.324550.

3D structure databases

ProteinModelPortaliQ9DBN5.
SMRiQ9DBN5. Positions 269-835.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211789. 1 interaction.
IntActiQ9DBN5. 1 interaction.
MINTiMINT-4128330.
STRINGi10090.ENSMUSP00000034141.

PTM databases

iPTMnetiQ9DBN5.
PhosphoSiteiQ9DBN5.
SwissPalmiQ9DBN5.

Proteomic databases

EPDiQ9DBN5.
MaxQBiQ9DBN5.
PaxDbiQ9DBN5.
PeptideAtlasiQ9DBN5.
PRIDEiQ9DBN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034141; ENSMUSP00000034141; ENSMUSG00000047866. [Q9DBN5-1]
ENSMUST00000122188; ENSMUSP00000113834; ENSMUSG00000047866. [Q9DBN5-3]
ENSMUST00000155433; ENSMUSP00000118737; ENSMUSG00000047866. [Q9DBN5-2]
GeneIDi66887.
KEGGimmu:66887.
UCSCiuc009mqm.3. mouse. [Q9DBN5-1]

Organism-specific databases

CTDi83752.
MGIiMGI:1914137. Lonp2.

Phylogenomic databases

eggNOGiKOG2004. Eukaryota.
COG0466. LUCA.
GeneTreeiENSGT00530000063553.
HOGENOMiHOG000261408.
HOVERGENiHBG000798.
InParanoidiQ9DBN5.
KOiK01338.
OMAiFVYGLAW.
OrthoDBiEOG77T13V.
PhylomeDBiQ9DBN5.
TreeFamiTF317215.

Miscellaneous databases

ChiTaRSiLonp2. mouse.
PROiQ9DBN5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DBN5.
CleanExiMM_LONP2.
ExpressionAtlasiQ9DBN5. baseline and differential.
GenevisibleiQ9DBN5. MM.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03121. lonp2_euk.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027501. Lonp2_euk.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
PS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Amnion, Bone marrow, Liver and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiLONP2_MOUSE
AccessioniPrimary (citable) accession number: Q9DBN5
Secondary accession number(s): Q3TEG8
, Q3TFY6, Q3TGR0, Q3UAE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.