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Q9DBM0

- ABCG8_MOUSE

UniProt

Q9DBM0 - ABCG8_MOUSE

Protein

ATP-binding cassette sub-family G member 8

Gene

Abcg8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.

    GO - Molecular functioni

    1. ATPase activity Source: Ensembl
    2. ATP binding Source: Ensembl
    3. cholesterol transporter activity Source: Ensembl

    GO - Biological processi

    1. cholesterol efflux Source: BHF-UCL
    2. cholesterol homeostasis Source: MGI
    3. excretion Source: BHF-UCL
    4. intestinal cholesterol absorption Source: BHF-UCL
    5. negative regulation of intestinal cholesterol absorption Source: Ensembl
    6. negative regulation of intestinal phytosterol absorption Source: Ensembl
    7. phospholipid transport Source: MGI
    8. response to drug Source: Ensembl
    9. response to nutrient Source: Ensembl
    10. sterol homeostasis Source: MGI
    11. sterol transport Source: MGI

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_197984. ABCA transporters in lipid homeostasis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-binding cassette sub-family G member 8
    Alternative name(s):
    Sterolin-2
    Gene namesi
    Name:Abcg8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1914720. Abcg8.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: BHF-UCL
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 673673ATP-binding cassette sub-family G member 8PRO_0000093397Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi619 – 6191N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ9DBM0.

    PTM databases

    PhosphoSiteiQ9DBM0.

    Expressioni

    Tissue specificityi

    Expressed in the intestine and, at lower level, in the liver.1 Publication

    Inductioni

    Up-regulated by cholesterol feeding. Possibly mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Endotoxin (LPS) significantly decreased mRNA levels in the liver but not in the small intestine.1 Publication

    Gene expression databases

    BgeeiQ9DBM0.
    GenevestigatoriQ9DBM0.

    Interactioni

    Subunit structurei

    May form heterodimers with ABCG5 or be tightly coupled to ABCG5 along a pathway regulating diatery-sterol absorption and excretion.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DBM0.
    SMRiQ9DBM0. Positions 46-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 413413CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini435 – 44713ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini469 – 49628CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini518 – 5269ExtracellularSequence Analysis
    Topological domaini548 – 56922CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini591 – 63949ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini661 – 67313CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei414 – 43421Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei448 – 46821Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei497 – 51721Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei527 – 54721Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei570 – 59021Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei640 – 66021Helical; Name=6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini48 – 314267ABC transporterPROSITE-ProRule annotationAdd
    BLAST
    Domaini411 – 665255ABC transmembrane type-2Add
    BLAST

    Sequence similaritiesi

    Contains 1 ABC transporter domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1131.
    GeneTreeiENSGT00740000114855.
    HOGENOMiHOG000033764.
    HOVERGENiHBG050444.
    InParanoidiQ9DBM0.
    KOiK05684.
    OMAiKCHSERS.
    PhylomeDBiQ9DBM0.
    TreeFamiTF105212.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR013525. ABC_2_trans.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01061. ABC2_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9DBM0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEKTKEETQ LWNGTVLQDA SQGLQDSLFS SESDNSLYFT YSGQSNTLEV    50
    RDLTYQVDIA SQVPWFEQLA QFKIPWRSHS SQDSCELGIR NLSFKVRSGQ 100
    MLAIIGSSGC GRASLLDVIT GRGHGGKMKS GQIWINGQPS TPQLVRKCVA 150
    HVRQHDQLLP NLTVRETLAF IAQMRLPRTF SQAQRDKRVE DVIAELRLRQ 200
    CANTRVGNTY VRGVSGGERR RVSIGVQLLW NPGILILDEP TSGLDSFTAH 250
    NLVTTLSRLA KGNRLVLISL HQPRSDIFRL FDLVLLMTSG TPIYLGAAQQ 300
    MVQYFTSIGH PCPRYSNPAD FYVDLTSIDR RSKEREVATV EKAQSLAALF 350
    LEKVQGFDDF LWKAEAKELN TSTHTVSLTL TQDTDCGTAV ELPGMIEQFS 400
    TLIRRQISND FRDLPTLLIH GSEACLMSLI IGFLYYGHGA KQLSFMDTAA 450
    LLFMIGALIP FNVILDVVSK CHSERSMLYY ELEDGLYTAG PYFFAKILGE 500
    LPEHCAYVII YAMPIYWLTN LRPVPELFLL HFLLVWLVVF CCRTMALAAS 550
    AMLPTFHMSS FFCNALYNSF YLTAGFMINL DNLWIVPAWI SKLSFLRWCF 600
    SGLMQIQFNG HLYTTQIGNF TFSILGDTMI SAMDLNSHPL YAIYLIVIGI 650
    SYGFLFLYYL SLKLIKQKSI QDW 673
    Length:673
    Mass (Da):75,996
    Last modified:June 1, 2001 - v1
    Checksum:i78012611A5DF2589
    GO
    Isoform 2 (identifier: Q9DBM0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         377-377: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:672
    Mass (Da):75,909
    Checksum:iFC57D60AB2D79D1F
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei377 – 3771Missing in isoform 2. 1 PublicationVSP_000053

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF324495 mRNA. Translation: AAK84079.1.
    AK004871 mRNA. Translation: BAB23630.1.
    CCDSiCCDS29002.1. [Q9DBM0-1]
    RefSeqiNP_001272934.1. NM_001286005.1.
    NP_080456.1. NM_026180.3. [Q9DBM0-1]
    UniGeneiMm.26581.

    Genome annotation databases

    EnsembliENSMUST00000045714; ENSMUSP00000035246; ENSMUSG00000024254. [Q9DBM0-1]
    GeneIDi67470.
    KEGGimmu:67470.
    UCSCiuc008dta.1. mouse. [Q9DBM0-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF324495 mRNA. Translation: AAK84079.1 .
    AK004871 mRNA. Translation: BAB23630.1 .
    CCDSi CCDS29002.1. [Q9DBM0-1 ]
    RefSeqi NP_001272934.1. NM_001286005.1.
    NP_080456.1. NM_026180.3. [Q9DBM0-1 ]
    UniGenei Mm.26581.

    3D structure databases

    ProteinModelPortali Q9DBM0.
    SMRi Q9DBM0. Positions 46-323.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9DBM0.

    Proteomic databases

    PRIDEi Q9DBM0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045714 ; ENSMUSP00000035246 ; ENSMUSG00000024254 . [Q9DBM0-1 ]
    GeneIDi 67470.
    KEGGi mmu:67470.
    UCSCi uc008dta.1. mouse. [Q9DBM0-1 ]

    Organism-specific databases

    CTDi 64241.
    MGIi MGI:1914720. Abcg8.

    Phylogenomic databases

    eggNOGi COG1131.
    GeneTreei ENSGT00740000114855.
    HOGENOMi HOG000033764.
    HOVERGENi HBG050444.
    InParanoidi Q9DBM0.
    KOi K05684.
    OMAi KCHSERS.
    PhylomeDBi Q9DBM0.
    TreeFami TF105212.

    Enzyme and pathway databases

    Reactomei REACT_197984. ABCA transporters in lipid homeostasis.

    Miscellaneous databases

    NextBioi 324674.
    PROi Q9DBM0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9DBM0.
    Genevestigatori Q9DBM0.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR013525. ABC_2_trans.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF01061. ABC2_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two genes that map to the STSL locus cause sitosterolemia: genomic structure and spectrum of mutations involving sterolin-1 and sterolin-2, encoded by ABCG5 and ABCG8, respectively."
      Lu K., Lee M.-H., Hazard S., Brooks-Wilson A., Hidaka H., Kojima H., Ose L., Stalenhoef A.F.H., Mietinnen T., Bjorkhem I., Bruckert E., Pandya A., Brewer H.B. Jr., Salen G., Dean M., Srivastava A.K., Patel S.B.
      Am. J. Hum. Genet. 69:278-290(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6.
      Tissue: Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Liver.
    3. "Accumulation of dietary cholesterol in sitosterolemia caused by mutations in adjacent ABC transporters."
      Berge K.E., Tian H., Graf G.A., Yu L., Grishin N.V., Schultz J., Kwiterovich P., Shan B., Barnes R., Hobbs H.H.
      Science 290:1771-1775(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    4. "Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR."
      Khovidhunkit W., Moser A.H., Shigenaga J.K., Grunfeld C., Feingold K.R.
      J. Lipid Res. 44:1728-1736(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOWN-REGULATION BY ENDOTOXIN.

    Entry informationi

    Entry nameiABCG8_MOUSE
    AccessioniPrimary (citable) accession number: Q9DBM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 5, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3