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Q9DBM0 (ABCG8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-binding cassette sub-family G member 8
Alternative name(s):
Sterolin-2
Gene names
Name:Abcg8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length673 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.

Subunit structure

May form heterodimers with ABCG5 or be tightly coupled to ABCG5 along a pathway regulating diatery-sterol absorption and excretion By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Tissue specificity

Expressed in the intestine and, at lower level, in the liver. Ref.3

Induction

Up-regulated by cholesterol feeding. Possibly mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Endotoxin (LPS) significantly decreased mRNA levels in the liver but not in the small intestine. Ref.3 Ref.4

Sequence similarities

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. [View classification]

Contains 1 ABC transmembrane type-2 domain.

Contains 1 ABC transporter domain.

Caution

Seems to have a defective ATP-binding region.

Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcholesterol efflux

Inferred from mutant phenotype PubMed 14504269. Source: BHF-UCL

cholesterol homeostasis

Inferred from mutant phenotype PubMed 15040800. Source: MGI

excretion

Inferred from mutant phenotype PubMed 14504269. Source: BHF-UCL

intestinal cholesterol absorption

Inferred by curator PubMed 12208868. Source: BHF-UCL

negative regulation of intestinal cholesterol absorption

Inferred from electronic annotation. Source: Ensembl

negative regulation of intestinal phytosterol absorption

Inferred from electronic annotation. Source: Ensembl

phospholipid transport

Inferred from mutant phenotype PubMed 15040800. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to nutrient

Inferred from electronic annotation. Source: Ensembl

sterol homeostasis

Inferred from mutant phenotype PubMed 15040800. Source: MGI

sterol transport

Inferred from mutant phenotype PubMed 15040800. Source: MGI

   Cellular_componentapical plasma membrane

Inferred from mutant phenotype PubMed 14504269. Source: BHF-UCL

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: Ensembl

ATPase activity

Inferred from electronic annotation. Source: Ensembl

cholesterol transporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9DBM0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9DBM0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     377-377: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 673673ATP-binding cassette sub-family G member 8
PRO_0000093397

Regions

Topological domain1 – 413413Cytoplasmic Potential
Transmembrane414 – 43421Helical; Name=1; Potential
Topological domain435 – 44713Extracellular Potential
Transmembrane448 – 46821Helical; Name=2; Potential
Topological domain469 – 49628Cytoplasmic Potential
Transmembrane497 – 51721Helical; Name=3; Potential
Topological domain518 – 5269Extracellular Potential
Transmembrane527 – 54721Helical; Name=4; Potential
Topological domain548 – 56922Cytoplasmic Potential
Transmembrane570 – 59021Helical; Name=5; Potential
Topological domain591 – 63949Extracellular Potential
Transmembrane640 – 66021Helical; Name=6; Potential
Topological domain661 – 67313Cytoplasmic Potential
Domain48 – 314267ABC transporter
Domain411 – 665255ABC transmembrane type-2

Amino acid modifications

Glycosylation6191N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence3771Missing in isoform 2.
VSP_000053

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 78012611A5DF2589

FASTA67375,996
        10         20         30         40         50         60 
MAEKTKEETQ LWNGTVLQDA SQGLQDSLFS SESDNSLYFT YSGQSNTLEV RDLTYQVDIA 

        70         80         90        100        110        120 
SQVPWFEQLA QFKIPWRSHS SQDSCELGIR NLSFKVRSGQ MLAIIGSSGC GRASLLDVIT 

       130        140        150        160        170        180 
GRGHGGKMKS GQIWINGQPS TPQLVRKCVA HVRQHDQLLP NLTVRETLAF IAQMRLPRTF 

       190        200        210        220        230        240 
SQAQRDKRVE DVIAELRLRQ CANTRVGNTY VRGVSGGERR RVSIGVQLLW NPGILILDEP 

       250        260        270        280        290        300 
TSGLDSFTAH NLVTTLSRLA KGNRLVLISL HQPRSDIFRL FDLVLLMTSG TPIYLGAAQQ 

       310        320        330        340        350        360 
MVQYFTSIGH PCPRYSNPAD FYVDLTSIDR RSKEREVATV EKAQSLAALF LEKVQGFDDF 

       370        380        390        400        410        420 
LWKAEAKELN TSTHTVSLTL TQDTDCGTAV ELPGMIEQFS TLIRRQISND FRDLPTLLIH 

       430        440        450        460        470        480 
GSEACLMSLI IGFLYYGHGA KQLSFMDTAA LLFMIGALIP FNVILDVVSK CHSERSMLYY 

       490        500        510        520        530        540 
ELEDGLYTAG PYFFAKILGE LPEHCAYVII YAMPIYWLTN LRPVPELFLL HFLLVWLVVF 

       550        560        570        580        590        600 
CCRTMALAAS AMLPTFHMSS FFCNALYNSF YLTAGFMINL DNLWIVPAWI SKLSFLRWCF 

       610        620        630        640        650        660 
SGLMQIQFNG HLYTTQIGNF TFSILGDTMI SAMDLNSHPL YAIYLIVIGI SYGFLFLYYL 

       670 
SLKLIKQKSI QDW 

« Hide

Isoform 2 [UniParc].

Checksum: FC57D60AB2D79D1F
Show »

FASTA67275,909

References

« Hide 'large scale' references
[1]"Two genes that map to the STSL locus cause sitosterolemia: genomic structure and spectrum of mutations involving sterolin-1 and sterolin-2, encoded by ABCG5 and ABCG8, respectively."
Lu K., Lee M.-H., Hazard S., Brooks-Wilson A., Hidaka H., Kojima H., Ose L., Stalenhoef A.F.H., Mietinnen T., Bjorkhem I., Bruckert E., Pandya A., Brewer H.B. Jr., Salen G., Dean M., Srivastava A.K., Patel S.B.
Am. J. Hum. Genet. 69:278-290(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6.
Tissue: Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Liver.
[3]"Accumulation of dietary cholesterol in sitosterolemia caused by mutations in adjacent ABC transporters."
Berge K.E., Tian H., Graf G.A., Yu L., Grishin N.V., Schultz J., Kwiterovich P., Shan B., Barnes R., Hobbs H.H.
Science 290:1771-1775(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[4]"Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR."
Khovidhunkit W., Moser A.H., Shigenaga J.K., Grunfeld C., Feingold K.R.
J. Lipid Res. 44:1728-1736(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DOWN-REGULATION BY ENDOTOXIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF324495 mRNA. Translation: AAK84079.1.
AK004871 mRNA. Translation: BAB23630.1.
RefSeqNP_001272934.1. NM_001286005.1.
NP_080456.1. NM_026180.3.
UniGeneMm.26581.

3D structure databases

ProteinModelPortalQ9DBM0.
SMRQ9DBM0. Positions 31-323.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9DBM0.

Proteomic databases

PRIDEQ9DBM0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045714; ENSMUSP00000035246; ENSMUSG00000024254. [Q9DBM0-1]
GeneID67470.
KEGGmmu:67470.
UCSCuc008dta.1. mouse. [Q9DBM0-1]

Organism-specific databases

CTD64241.
MGIMGI:1914720. Abcg8.

Phylogenomic databases

eggNOGCOG1131.
GeneTreeENSGT00740000114855.
HOGENOMHOG000033764.
HOVERGENHBG050444.
InParanoidQ9DBM0.
KOK05684.
OMADVVSKCH.
PhylomeDBQ9DBM0.
TreeFamTF105212.

Gene expression databases

BgeeQ9DBM0.
GenevestigatorQ9DBM0.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio324674.
PROQ9DBM0.
SOURCESearch...

Entry information

Entry nameABCG8_MOUSE
AccessionPrimary (citable) accession number: Q9DBM0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot