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Protein

ATP-binding cassette sub-family G member 8

Gene

Abcg8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.

GO - Molecular functioni

GO - Biological processi

  • cholesterol efflux Source: BHF-UCL
  • cholesterol homeostasis Source: MGI
  • excretion Source: BHF-UCL
  • intestinal cholesterol absorption Source: BHF-UCL
  • negative regulation of intestinal cholesterol absorption Source: MGI
  • negative regulation of intestinal phytosterol absorption Source: MGI
  • phospholipid transport Source: MGI
  • response to drug Source: Ensembl
  • response to nutrient Source: Ensembl
  • sterol homeostasis Source: MGI
  • sterol transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-1369062. ABC transporters in lipid homeostasis.
R-MMU-265473. Trafficking of dietary sterols.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family G member 8
Alternative name(s):
Sterolin-2
Gene namesi
Name:Abcg8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1914720. Abcg8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 413CytoplasmicSequence analysisAdd BLAST413
Transmembranei414 – 434Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini435 – 447ExtracellularSequence analysisAdd BLAST13
Transmembranei448 – 468Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini469 – 496CytoplasmicSequence analysisAdd BLAST28
Transmembranei497 – 517Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini518 – 526ExtracellularSequence analysis9
Transmembranei527 – 547Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini548 – 569CytoplasmicSequence analysisAdd BLAST22
Transmembranei570 – 590Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini591 – 639ExtracellularSequence analysisAdd BLAST49
Transmembranei640 – 660Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini661 – 673CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • apical plasma membrane Source: BHF-UCL
  • ATP-binding cassette (ABC) transporter complex Source: MGI
  • integral component of membrane Source: GO_Central
  • receptor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933971 – 673ATP-binding cassette sub-family G member 8Add BLAST673

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi619N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9DBM0.
PRIDEiQ9DBM0.

PTM databases

iPTMnetiQ9DBM0.
PhosphoSitePlusiQ9DBM0.

Expressioni

Tissue specificityi

Expressed in the intestine and, at lower level, in the liver.1 Publication

Inductioni

Up-regulated by cholesterol feeding. Possibly mediated by the liver X receptor/retinoid X receptor (LXR/RXR) pathway. Endotoxin (LPS) significantly decreased mRNA levels in the liver but not in the small intestine.1 Publication

Gene expression databases

BgeeiENSMUSG00000024254.
ExpressionAtlasiQ9DBM0. baseline and differential.
GenevisibleiQ9DBM0. MM.

Interactioni

Subunit structurei

May form heterodimers with ABCG5 or be tightly coupled to ABCG5 along a pathway regulating diatery-sterol absorption and excretion.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035246.

Structurei

3D structure databases

ProteinModelPortaliQ9DBM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 314ABC transporterPROSITE-ProRule annotationAdd BLAST267
Domaini411 – 665ABC transmembrane type-2Add BLAST255

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0061. Eukaryota.
COG1131. LUCA.
GeneTreeiENSGT00740000114855.
HOGENOMiHOG000033764.
HOVERGENiHBG050444.
InParanoidiQ9DBM0.
KOiK05684.
OMAiDVVSKCH.
OrthoDBiEOG091G0E38.
PhylomeDBiQ9DBM0.
TreeFamiTF105212.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DBM0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEKTKEETQ LWNGTVLQDA SQGLQDSLFS SESDNSLYFT YSGQSNTLEV
60 70 80 90 100
RDLTYQVDIA SQVPWFEQLA QFKIPWRSHS SQDSCELGIR NLSFKVRSGQ
110 120 130 140 150
MLAIIGSSGC GRASLLDVIT GRGHGGKMKS GQIWINGQPS TPQLVRKCVA
160 170 180 190 200
HVRQHDQLLP NLTVRETLAF IAQMRLPRTF SQAQRDKRVE DVIAELRLRQ
210 220 230 240 250
CANTRVGNTY VRGVSGGERR RVSIGVQLLW NPGILILDEP TSGLDSFTAH
260 270 280 290 300
NLVTTLSRLA KGNRLVLISL HQPRSDIFRL FDLVLLMTSG TPIYLGAAQQ
310 320 330 340 350
MVQYFTSIGH PCPRYSNPAD FYVDLTSIDR RSKEREVATV EKAQSLAALF
360 370 380 390 400
LEKVQGFDDF LWKAEAKELN TSTHTVSLTL TQDTDCGTAV ELPGMIEQFS
410 420 430 440 450
TLIRRQISND FRDLPTLLIH GSEACLMSLI IGFLYYGHGA KQLSFMDTAA
460 470 480 490 500
LLFMIGALIP FNVILDVVSK CHSERSMLYY ELEDGLYTAG PYFFAKILGE
510 520 530 540 550
LPEHCAYVII YAMPIYWLTN LRPVPELFLL HFLLVWLVVF CCRTMALAAS
560 570 580 590 600
AMLPTFHMSS FFCNALYNSF YLTAGFMINL DNLWIVPAWI SKLSFLRWCF
610 620 630 640 650
SGLMQIQFNG HLYTTQIGNF TFSILGDTMI SAMDLNSHPL YAIYLIVIGI
660 670
SYGFLFLYYL SLKLIKQKSI QDW
Length:673
Mass (Da):75,996
Last modified:June 1, 2001 - v1
Checksum:i78012611A5DF2589
GO
Isoform 2 (identifier: Q9DBM0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     377-377: Missing.

Note: No experimental confirmation available.
Show »
Length:672
Mass (Da):75,909
Checksum:iFC57D60AB2D79D1F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000053377Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF324495 mRNA. Translation: AAK84079.1.
AK004871 mRNA. Translation: BAB23630.1.
CCDSiCCDS29002.1. [Q9DBM0-1]
RefSeqiNP_001272934.1. NM_001286005.1.
NP_080456.1. NM_026180.3. [Q9DBM0-1]
UniGeneiMm.26581.

Genome annotation databases

EnsembliENSMUST00000045714; ENSMUSP00000035246; ENSMUSG00000024254. [Q9DBM0-1]
GeneIDi67470.
KEGGimmu:67470.
UCSCiuc008dta.2. mouse. [Q9DBM0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF324495 mRNA. Translation: AAK84079.1.
AK004871 mRNA. Translation: BAB23630.1.
CCDSiCCDS29002.1. [Q9DBM0-1]
RefSeqiNP_001272934.1. NM_001286005.1.
NP_080456.1. NM_026180.3. [Q9DBM0-1]
UniGeneiMm.26581.

3D structure databases

ProteinModelPortaliQ9DBM0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035246.

PTM databases

iPTMnetiQ9DBM0.
PhosphoSitePlusiQ9DBM0.

Proteomic databases

PaxDbiQ9DBM0.
PRIDEiQ9DBM0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045714; ENSMUSP00000035246; ENSMUSG00000024254. [Q9DBM0-1]
GeneIDi67470.
KEGGimmu:67470.
UCSCiuc008dta.2. mouse. [Q9DBM0-1]

Organism-specific databases

CTDi64241.
MGIiMGI:1914720. Abcg8.

Phylogenomic databases

eggNOGiKOG0061. Eukaryota.
COG1131. LUCA.
GeneTreeiENSGT00740000114855.
HOGENOMiHOG000033764.
HOVERGENiHBG050444.
InParanoidiQ9DBM0.
KOiK05684.
OMAiDVVSKCH.
OrthoDBiEOG091G0E38.
PhylomeDBiQ9DBM0.
TreeFamiTF105212.

Enzyme and pathway databases

ReactomeiR-MMU-1369062. ABC transporters in lipid homeostasis.
R-MMU-265473. Trafficking of dietary sterols.

Miscellaneous databases

PROiQ9DBM0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024254.
ExpressionAtlasiQ9DBM0. baseline and differential.
GenevisibleiQ9DBM0. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR013525. ABC_2_trans.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01061. ABC2_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiABCG8_MOUSE
AccessioniPrimary (citable) accession number: Q9DBM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Seems to have a defective ATP-binding region.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.