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Protein

Acyl-coenzyme A thioesterase 12

Gene

Acot12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes acetyl-CoA to acetate and CoA.By similarity

Catalytic activityi

Acetyl-CoA + H2O = CoA + acetate.

Pathwayi: pyruvate metabolism

This protein is involved in the pathway pyruvate metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway pyruvate metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei145Coenzyme ABy similarity1

GO - Molecular functioni

  • acetyl-CoA hydrolase activity Source: MGI
  • acyl-CoA hydrolase activity Source: GO_Central
  • ATP binding Source: MGI
  • carboxylic ester hydrolase activity Source: UniProtKB-KW
  • lipid binding Source: InterPro

GO - Biological processi

  • acetyl-CoA metabolic process Source: MGI
  • fatty acid metabolic process Source: MGI
  • pyruvate metabolic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processFatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-77289. Mitochondrial Fatty Acid Beta-Oxidation.
UniPathwayiUPA00231.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 12 (EC:3.1.2.1)
Short name:
Acyl-CoA thioesterase 12
Alternative name(s):
Acyl-CoA thioester hydrolase 12
Cytoplasmic acetyl-CoA hydrolase 1
Short name:
CACH-1
Short name:
mCACH-1
Gene namesi
Name:Acot12
Synonyms:Cach, Cach1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1921406. Acot12.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538101 – 556Acyl-coenzyme A thioesterase 12Add BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34N6-succinyllysineCombined sources1
Modified residuei97N6-succinyllysineCombined sources1
Modified residuei160N6-succinyllysineCombined sources1
Modified residuei229N6-succinyllysineCombined sources1

Proteomic databases

MaxQBiQ9DBK0.
PaxDbiQ9DBK0.
PRIDEiQ9DBK0.

PTM databases

iPTMnetiQ9DBK0.
PhosphoSitePlusiQ9DBK0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021620.
CleanExiMM_ACOT12.
ExpressionAtlasiQ9DBK0. baseline and differential.
GenevisibleiQ9DBK0. MM.

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

IntActiQ9DBK0. 3 interactors.
MINTiMINT-4089645.
STRINGi10090.ENSMUSP00000022120.

Structurei

3D structure databases

ProteinModelPortaliQ9DBK0.
SMRiQ9DBK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 118HotDog ACOT-type 1PROSITE-ProRule annotationAdd BLAST113
Domaini180 – 295HotDog ACOT-type 2PROSITE-ProRule annotationAdd BLAST116
Domaini327 – 536STARTPROSITE-ProRule annotationAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 56Coenzyme A bindingBy similarity3
Regioni83 – 85Coenzyme A bindingBy similarity3
Regioni235 – 237Coenzyme A bindingBy similarity3

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2763. Eukaryota.
COG1607. LUCA.
GeneTreeiENSGT00760000119297.
HOGENOMiHOG000232032.
HOVERGENiHBG023847.
InParanoidiQ9DBK0.
KOiK01067.
OMAiRPLWDPH.
OrthoDBiEOG091G06LZ.
PhylomeDBiQ9DBK0.
TreeFamiTF328368.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiView protein in InterPro
IPR033120. HOTDOG_ACOT.
IPR029069. HotDog_dom.
IPR023393. START-like_dom.
IPR002913. START_lipid-bd_dom.
IPR006683. Thioestr_dom.
PfamiView protein in Pfam
PF03061. 4HBT. 2 hits.
PF01852. START. 1 hit.
SMARTiView protein in SMART
SM00234. START. 1 hit.
SUPFAMiSSF54637. SSF54637. 2 hits.
PROSITEiView protein in PROSITE
PS51770. HOTDOG_ACOT. 2 hits.
PS50848. START. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DBK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESMVAPGEV LMSQAIQPAH ADSRGELSAG QLLKWMDTTA CLAAEKHAGI
60 70 80 90 100
SCVTASMDDI LFEDTARIGQ IITIRAKVTR AFSTSMEISI KVIVQDKFTG
110 120 130 140 150
IQKLLCVAFS TFVAKPVGKE KVHLKPVLLQ TEQEQVEHNL ASERRKVRLQ
160 170 180 190 200
HENTFNNIMK ESSRFSDSIC NEEEGTATTM GTSVQSIELV LPPHANHHGN
210 220 230 240 250
TFGGQIMAWM ETVATISASR LCHGHPFLKS VDMFKFRGPS TVGDRLVFSA
260 270 280 290 300
IVNNTFQNSV EVGVRVEAFD CQEWAEGQGR HINSAFLIYN AVDDQEKLIT
310 320 330 340 350
FPRIQPISKD DFRRYQGAIA RRRIRLGRKY VISHKKEVPL SAQWDISKKG
360 370 380 390 400
SLSNTNVEAL KNLASKSGWE ITTTLEKIKI YTLEEQDAIS VKVEKLVGSP
410 420 430 440 450
AHIAYHLLSD LTKRPLWDPH YISCEVIDQV SEDDQIYYIT CSVVNGDKPK
460 470 480 490 500
DFVVLVSRRK PLKDNNTYTV ALRSVVLPSV PSSPQYIRSE VICAGFLIQA
510 520 530 540 550
VDSNSCTVTY LNQMSDSILP YFAGNIGGWS KSIEEAAASC IKFIENATPD

GLKSVL
Length:556
Mass (Da):61,762
Last modified:June 1, 2001 - v1
Checksum:i9FE9C487BBCBB812
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078618 mRNA. Translation: BAB84021.1.
AK004905 mRNA. Translation: BAB23658.1.
AK034622 mRNA. Translation: BAC28775.1.
BC025852 mRNA. Translation: AAH25852.1.
CCDSiCCDS26678.1.
RefSeqiNP_083066.1. NM_028790.3.
UniGeneiMm.275963.

Genome annotation databases

EnsembliENSMUST00000022120; ENSMUSP00000022120; ENSMUSG00000021620.
GeneIDi74156.
KEGGimmu:74156.
UCSCiuc007rka.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiACO12_MOUSE
AccessioniPrimary (citable) accession number: Q9DBK0
Secondary accession number(s): Q544M5, Q8R108
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: June 1, 2001
Last modified: July 5, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways