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Q9DBJ6

- JOS1_MOUSE

UniProt

Q9DBJ6 - JOS1_MOUSE

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Protein

Josephin-1

Gene

Josd1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity By similarity.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361NucleophilePROSITE-ProRule annotation
Active sitei139 – 1391Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. omega peptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC86.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-1 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 1
Gene namesi
Name:Josd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1921408. Josd1.

Subcellular locationi

Cell membrane By similarity. Cytoplasm By similarity
Note: Ubiquitination increases localization the plasma membrane. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Josephin-1PRO_0000053840Add
BLAST

Post-translational modificationi

Monoubiquitinated. Ubiquitination activates deubiquitination activity in vitro.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9DBJ6.
PRIDEiQ9DBJ6.

PTM databases

PhosphoSiteiQ9DBJ6.

Expressioni

Tissue specificityi

Widely expressed (at protein level).1 Publication

Gene expression databases

BgeeiQ9DBJ6.
CleanExiMM_JOSD1.
GenevestigatoriQ9DBJ6.

Interactioni

Subunit structurei

Interacts with beta-actin/ACTB.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9DBJ6.
SMRiQ9DBJ6. Positions 26-163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 202180JosephinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Josephin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG321724.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ9DBJ6.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG75B871.
PhylomeDBiQ9DBJ6.
TreeFamiTF313660.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DBJ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSCVPWKGDK AKAESSDLPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA
60 70 80 90 100
FTRETLQEIF QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW
110 120 130 140 150
WDKRRDVGVI ALTNVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY
160 170 180 190 200
YNLDSKLKMP EWIGGESELR KFLKYHLRGK NCELLLVVPE EVEAHQSWRA

DV
Length:202
Mass (Da):23,152
Last modified:June 1, 2001 - v1
Checksum:i8F04FB5C37529BF7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK004913 mRNA. Translation: BAB23664.1.
AK154799 mRNA. Translation: BAE32837.1.
BC006928 mRNA. Translation: AAH06928.1.
BC086769 mRNA. Translation: AAH86769.1.
CCDSiCCDS27647.1.
RefSeqiNP_083068.1. NM_028792.3.
XP_001481034.3. XM_001480984.4.
XP_006544219.1. XM_006544156.1.
UniGeneiMm.323393.
Mm.491099.

Genome annotation databases

EnsembliENSMUST00000023061; ENSMUSP00000023061; ENSMUSG00000022426.
GeneIDi100043771.
74158.
KEGGimmu:100043771.
mmu:74158.
UCSCiuc007wue.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK004913 mRNA. Translation: BAB23664.1 .
AK154799 mRNA. Translation: BAE32837.1 .
BC006928 mRNA. Translation: AAH06928.1 .
BC086769 mRNA. Translation: AAH86769.1 .
CCDSi CCDS27647.1.
RefSeqi NP_083068.1. NM_028792.3.
XP_001481034.3. XM_001480984.4.
XP_006544219.1. XM_006544156.1.
UniGenei Mm.323393.
Mm.491099.

3D structure databases

ProteinModelPortali Q9DBJ6.
SMRi Q9DBJ6. Positions 26-163.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C86.004.

PTM databases

PhosphoSitei Q9DBJ6.

Proteomic databases

PaxDbi Q9DBJ6.
PRIDEi Q9DBJ6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000023061 ; ENSMUSP00000023061 ; ENSMUSG00000022426 .
GeneIDi 100043771.
74158.
KEGGi mmu:100043771.
mmu:74158.
UCSCi uc007wue.2. mouse.

Organism-specific databases

CTDi 9929.
MGIi MGI:1921408. Josd1.

Phylogenomic databases

eggNOGi NOG321724.
GeneTreei ENSGT00390000009228.
HOGENOMi HOG000005731.
HOVERGENi HBG039523.
InParanoidi Q9DBJ6.
KOi K15235.
OMAi REVGGTY.
OrthoDBi EOG75B871.
PhylomeDBi Q9DBJ6.
TreeFami TF313660.

Miscellaneous databases

NextBioi 339942.
PROi Q9DBJ6.
SOURCEi Search...

Gene expression databases

Bgeei Q9DBJ6.
CleanExi MM_JOSD1.
Genevestigatori Q9DBJ6.

Family and domain databases

InterProi IPR006155. Josephin.
[Graphical view ]
Pfami PF02099. Josephin. 1 hit.
[Graphical view ]
PROSITEi PS50957. JOSEPHIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Mammary tumor.
  3. "JosD1, a membrane-targeted deubiquitinating enzyme, is activated by ubiquitination and regulates membrane dynamics, cell motility, and endocytosis."
    Seki T., Gong L., Williams A.J., Sakai N., Todi S.V., Paulson H.L.
    J. Biol. Chem. 288:17145-17155(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, UBIQUITINATION.
  4. "Structural modeling of ataxin-3 reveals distant homology to adaptins."
    Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T.
    Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiJOS1_MOUSE
AccessioniPrimary (citable) accession number: Q9DBJ6
Secondary accession number(s): Q3U3E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3