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Q9DBJ6

- JOS1_MOUSE

UniProt

Q9DBJ6 - JOS1_MOUSE

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Protein
Josephin-1
Gene
Josd1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361Nucleophile By similarity
Active sitei139 – 1391Proton acceptor By similarity

GO - Molecular functioni

  1. omega peptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC86.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Josephin-1 (EC:3.4.19.12)
    Alternative name(s):
    Josephin domain-containing protein 1
    Gene namesi
    Name:Josd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1921408. Josd1.

    Subcellular locationi

    Cell membrane By similarity. Cytoplasm By similarity
    Note: Ubiquitination increases localization the plasma membrane By similarity. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding By similarity.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 202202Josephin-1
    PRO_0000053840Add
    BLAST

    Post-translational modificationi

    Monoubiquitinated. Ubiquitination activates deubiquitination activity in vitro.

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ9DBJ6.
    PRIDEiQ9DBJ6.

    PTM databases

    PhosphoSiteiQ9DBJ6.

    Expressioni

    Tissue specificityi

    Widely expressed (at protein level).1 Publication

    Gene expression databases

    BgeeiQ9DBJ6.
    CleanExiMM_JOSD1.
    GenevestigatoriQ9DBJ6.

    Interactioni

    Subunit structurei

    Interacts with beta-actin/ACTB By similarity.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DBJ6.
    SMRiQ9DBJ6. Positions 26-163.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 202180Josephin
    Add
    BLAST

    Sequence similaritiesi

    Contains 1 Josephin domain.

    Phylogenomic databases

    eggNOGiNOG321724.
    GeneTreeiENSGT00390000009228.
    HOGENOMiHOG000005731.
    HOVERGENiHBG039523.
    InParanoidiQ9DBJ6.
    KOiK15235.
    OMAiREVGGTY.
    OrthoDBiEOG75B871.
    PhylomeDBiQ9DBJ6.
    TreeFamiTF313660.

    Family and domain databases

    InterProiIPR006155. Josephin.
    [Graphical view]
    PfamiPF02099. Josephin. 1 hit.
    [Graphical view]
    PROSITEiPS50957. JOSEPHIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9DBJ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCVPWKGDK AKAESSDLPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA    50
    FTRETLQEIF QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW 100
    WDKRRDVGVI ALTNVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY 150
    YNLDSKLKMP EWIGGESELR KFLKYHLRGK NCELLLVVPE EVEAHQSWRA 200
    DV 202
    Length:202
    Mass (Da):23,152
    Last modified:June 1, 2001 - v1
    Checksum:i8F04FB5C37529BF7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004913 mRNA. Translation: BAB23664.1.
    AK154799 mRNA. Translation: BAE32837.1.
    BC006928 mRNA. Translation: AAH06928.1.
    BC086769 mRNA. Translation: AAH86769.1.
    CCDSiCCDS27647.1.
    RefSeqiNP_083068.1. NM_028792.3.
    XP_001481034.3. XM_001480984.4.
    XP_006544219.1. XM_006544156.1.
    UniGeneiMm.323393.
    Mm.491099.

    Genome annotation databases

    EnsembliENSMUST00000023061; ENSMUSP00000023061; ENSMUSG00000022426.
    GeneIDi100043771.
    74158.
    KEGGimmu:100043771.
    mmu:74158.
    UCSCiuc007wue.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004913 mRNA. Translation: BAB23664.1 .
    AK154799 mRNA. Translation: BAE32837.1 .
    BC006928 mRNA. Translation: AAH06928.1 .
    BC086769 mRNA. Translation: AAH86769.1 .
    CCDSi CCDS27647.1.
    RefSeqi NP_083068.1. NM_028792.3.
    XP_001481034.3. XM_001480984.4.
    XP_006544219.1. XM_006544156.1.
    UniGenei Mm.323393.
    Mm.491099.

    3D structure databases

    ProteinModelPortali Q9DBJ6.
    SMRi Q9DBJ6. Positions 26-163.
    ModBasei Search...

    Protein family/group databases

    MEROPSi C86.004.

    PTM databases

    PhosphoSitei Q9DBJ6.

    Proteomic databases

    PaxDbi Q9DBJ6.
    PRIDEi Q9DBJ6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023061 ; ENSMUSP00000023061 ; ENSMUSG00000022426 .
    GeneIDi 100043771.
    74158.
    KEGGi mmu:100043771.
    mmu:74158.
    UCSCi uc007wue.2. mouse.

    Organism-specific databases

    CTDi 9929.
    MGIi MGI:1921408. Josd1.

    Phylogenomic databases

    eggNOGi NOG321724.
    GeneTreei ENSGT00390000009228.
    HOGENOMi HOG000005731.
    HOVERGENi HBG039523.
    InParanoidi Q9DBJ6.
    KOi K15235.
    OMAi REVGGTY.
    OrthoDBi EOG75B871.
    PhylomeDBi Q9DBJ6.
    TreeFami TF313660.

    Miscellaneous databases

    NextBioi 339942.
    PROi Q9DBJ6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9DBJ6.
    CleanExi MM_JOSD1.
    Genevestigatori Q9DBJ6.

    Family and domain databases

    InterProi IPR006155. Josephin.
    [Graphical view ]
    Pfami PF02099. Josephin. 1 hit.
    [Graphical view ]
    PROSITEi PS50957. JOSEPHIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain and Mammary tumor.
    3. "JosD1, a membrane-targeted deubiquitinating enzyme, is activated by ubiquitination and regulates membrane dynamics, cell motility, and endocytosis."
      Seki T., Gong L., Williams A.J., Sakai N., Todi S.V., Paulson H.L.
      J. Biol. Chem. 288:17145-17155(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, UBIQUITINATION.
    4. "Structural modeling of ataxin-3 reveals distant homology to adaptins."
      Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T.
      Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiJOS1_MOUSE
    AccessioniPrimary (citable) accession number: Q9DBJ6
    Secondary accession number(s): Q3U3E9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 28, 2003
    Last sequence update: June 1, 2001
    Last modified: July 9, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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