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Protein

Josephin-1

Gene

Josd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361NucleophilePROSITE-ProRule annotation
Active sitei139 – 1391Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. omega peptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC86.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-1 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 1
Gene namesi
Name:Josd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1921408. Josd1.

Subcellular locationi

Cell membrane By similarity. Cytoplasm By similarity
Note: Ubiquitination increases localization the plasma membrane. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Josephin-1PRO_0000053840Add
BLAST

Post-translational modificationi

Monoubiquitinated. Ubiquitination activates deubiquitination activity in vitro.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9DBJ6.
PRIDEiQ9DBJ6.

PTM databases

PhosphoSiteiQ9DBJ6.

Expressioni

Tissue specificityi

Widely expressed (at protein level).1 Publication

Gene expression databases

BgeeiQ9DBJ6.
CleanExiMM_JOSD1.
GenevestigatoriQ9DBJ6.

Interactioni

Subunit structurei

Interacts with beta-actin/ACTB.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9DBJ6.
SMRiQ9DBJ6. Positions 26-163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 202180JosephinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Josephin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG321724.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ9DBJ6.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG75B871.
PhylomeDBiQ9DBJ6.
TreeFamiTF313660.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DBJ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSCVPWKGDK AKAESSDLPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA
60 70 80 90 100
FTRETLQEIF QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW
110 120 130 140 150
WDKRRDVGVI ALTNVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY
160 170 180 190 200
YNLDSKLKMP EWIGGESELR KFLKYHLRGK NCELLLVVPE EVEAHQSWRA

DV
Length:202
Mass (Da):23,152
Last modified:June 1, 2001 - v1
Checksum:i8F04FB5C37529BF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004913 mRNA. Translation: BAB23664.1.
AK154799 mRNA. Translation: BAE32837.1.
BC006928 mRNA. Translation: AAH06928.1.
BC086769 mRNA. Translation: AAH86769.1.
CCDSiCCDS27647.1.
RefSeqiNP_083068.1. NM_028792.3.
XP_001481034.3. XM_001480984.4.
XP_006544219.1. XM_006544156.1.
UniGeneiMm.323393.
Mm.491099.

Genome annotation databases

EnsembliENSMUST00000023061; ENSMUSP00000023061; ENSMUSG00000022426.
GeneIDi100043771.
74158.
KEGGimmu:100043771.
mmu:74158.
UCSCiuc007wue.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004913 mRNA. Translation: BAB23664.1.
AK154799 mRNA. Translation: BAE32837.1.
BC006928 mRNA. Translation: AAH06928.1.
BC086769 mRNA. Translation: AAH86769.1.
CCDSiCCDS27647.1.
RefSeqiNP_083068.1. NM_028792.3.
XP_001481034.3. XM_001480984.4.
XP_006544219.1. XM_006544156.1.
UniGeneiMm.323393.
Mm.491099.

3D structure databases

ProteinModelPortaliQ9DBJ6.
SMRiQ9DBJ6. Positions 26-163.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC86.004.

PTM databases

PhosphoSiteiQ9DBJ6.

Proteomic databases

PaxDbiQ9DBJ6.
PRIDEiQ9DBJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023061; ENSMUSP00000023061; ENSMUSG00000022426.
GeneIDi100043771.
74158.
KEGGimmu:100043771.
mmu:74158.
UCSCiuc007wue.2. mouse.

Organism-specific databases

CTDi9929.
MGIiMGI:1921408. Josd1.

Phylogenomic databases

eggNOGiNOG321724.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ9DBJ6.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG75B871.
PhylomeDBiQ9DBJ6.
TreeFamiTF313660.

Miscellaneous databases

ChiTaRSiJosd1. mouse.
NextBioi339942.
PROiQ9DBJ6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DBJ6.
CleanExiMM_JOSD1.
GenevestigatoriQ9DBJ6.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Mammary tumor.
  3. "JosD1, a membrane-targeted deubiquitinating enzyme, is activated by ubiquitination and regulates membrane dynamics, cell motility, and endocytosis."
    Seki T., Gong L., Williams A.J., Sakai N., Todi S.V., Paulson H.L.
    J. Biol. Chem. 288:17145-17155(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, UBIQUITINATION.
  4. "Structural modeling of ataxin-3 reveals distant homology to adaptins."
    Albrecht M., Hoffmann D., Evert B.O., Schmitt I., Wuellner U., Lengauer T.
    Proteins 50:355-370(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiJOS1_MOUSE
AccessioniPrimary (citable) accession number: Q9DBJ6
Secondary accession number(s): Q3U3E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.