Q9DBJ3 (BI2L1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1
Short name=BAI1-associated protein 2-like protein 1

Alternative name(s):
Insulin receptor tyrosine kinase substrate

Gene names

Name:	Baiap2l1
Synonyms:	Irtks

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection By similarity.
Subunit structure	Interacts with RAC1. Binds to F-actin. Interacts with FASLG By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Note: Recruited to actin pedestals that are formed upon infection by bacteria at bacterial attachment sites By similarity.
Tissue specificity	Detected in bladder, liver, testes, heart, lung, spleen, brain and skeletal muscle (at protein level). Ref.5
Domain	The IMD domain is predicted to have a helical structure. It may induce actin bundling and filopodia formation By similarity.
Post-translational modification	Phosphorylated on tyrosine in response to insulin By similarity.
Sequence similarities	Contains 1 IMD (IRSp53/MIM homology) domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Coiled coil SH3 domain
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	filopodium assembly Inferred from electronic annotation. Source: InterPro positive regulation of actin cytoskeleton reorganization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of actin filament polymerization Inferred from sequence or structural similarity. Source: UniProtKB response to bacterium Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	actin cytoskeleton Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: Compara cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	proline-rich region binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 514

514

Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1

PRO_0000247855

Regions

Domain

1 – 249

249

IMD

Domain

340 – 403

64

SH3

Region

486 – 514

29

Binds F-actin By similarity

Coiled coil

115 – 148

34

Potential

Amino acid modifications

Modified residue

248

1

Phosphothreonine By similarity

Modified residue

257

1

Phosphothreonine By similarity

Modified residue

261

1

Phosphoserine Ref.3

Modified residue

281

1

Phosphoserine By similarity

Modified residue

330

1

Phosphoserine Ref.3

Modified residue

332

1

Phosphoserine Ref.3

Modified residue

411

1

Phosphothreonine By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

514

Helix Strand Turn

Details...

Beta strand

344 – 346

3

Beta strand

357 – 359

3

Beta strand

367 – 370

4

Beta strand

379 – 384

6

Turn

385 – 387

3

Beta strand

390 – 394

5

Helix

395 – 397

3

Sequences

	Sequence		Length	Mass (Da)
	Q9DBJ3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: AF9974A0696812EC	FASTA	514	57,188
	10 20 30 40 50 60 MSRGPEEVNR LTENTYRNVM EQFNPGLRNL INLGKNYEKA VNAMILAGKA YYDGVAKIGE 70 80 90 100 110 120 IATGSPVSTE LGHVLIEISS THKKLNETLD ENFKKFHKDI IHELEKKTEL DVKYMNATLK 130 140 150 160 170 180 RYQAEHRNKL DSLEKSQAEL KKIRRKSQGG RNALKYEHKE IEYVETVTSR QSEIQKFIAD 190 200 210 220 230 240 GCKEALLEEK RRFCFLVDKH CSFASHIHYY HMQSAELLNS KLPRWQETCC DATKVPEKIM 250 260 270 280 290 300 NMIEEIKTPI STPVSGTPQP SPMIERSKMI GKDYDTLSKY SPKMPPAPSV KAYTSPLIDM 310 320 330 340 350 360 FNNPATAAQS SEKTNNSTAN TGEDPSLQRS VSVATGLNMM KKQKVKTIFP HTAGNNKTLL 370 380 390 400 410 420 SFAQGDVLTL LIPEEKDGWL YGEHDTTKAR GWFPSSYTKL LEENEAMSVP TPSPAPVRSI 430 440 450 460 470 480 STVDLTEKSS VVIPPPDYLE CLSMGATSDK KAGAPKVPSA STFRAPVSRP DATSTSPSDA 490 500 510 NGTAKPPFLS GENPFATVKL RPTVTNDRSA PIIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-261; SER-330 AND SER-332, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Solution structure of RSGI RUH-010, an SH3 domain from mouse cDNA." RIKEN structural genomics initiative (RSGI) Submitted (SEP-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 343-401.
[5]	"Characterisation of IRTKS, a novel IRSp53/MIM family actin regulator with distinct filament bundling properties." Millard T.H., Dawson J., Machesky L.M. J. Cell Sci. 120:1663-1672(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK004918 mRNA. Translation: BAB23669.1.
BC015459 mRNA. Translation: AAH15459.1.

IPI

IPI00321643.

RefSeq

NP_080109.1. NM_025833.3.

UniGene

Mm.18814.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SPK	NMR	-	A	343-401	[»]

ProteinModelPortal

Q9DBJ3.

SMR

Q9DBJ3. Positions 6-227, 343-401.

ModBase

Search...

Protein-protein interaction databases

STRING

10090.ENSMUSP00000053129.

PTM databases

PhosphoSite

Q9DBJ3.

Proteomic databases

PaxDb

Q9DBJ3.

PRIDE

Q9DBJ3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000055190; ENSMUSP00000053129; ENSMUSG00000038859.

GeneID

66898.

KEGG

mmu:66898.

UCSC

uc009alq.1. mouse.

Organism-specific databases

CTD

55971.

MGI

MGI:1914148. Baiap2l1.

Phylogenomic databases

eggNOG

NOG71665.

GeneTree

ENSGT00390000005995.

HOGENOM

HOG000038005.

HOVERGEN

HBG054462.

InParanoid

Q9DBJ3.

OMA

TESTYRN.

OrthoDB

EOG4GXFMT.

Gene expression databases

Bgee

Q9DBJ3.

CleanEx

MM_BAIAP2L1.

Genevestigator

Q9DBJ3.

GermOnline

ENSMUSG00000038859. Mus musculus.

Family and domain databases

InterPro

IPR013606. IRSp53/MIM_homology_IMD.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF08397. IMD. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]

SMART

SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS51338. IMD. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9DBJ3.

NextBio

322961.

SOURCE

Search...

Entry information

Entry name

BI2L1_MOUSE

Accession

Primary (citable) accession number: Q9DBJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program