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Q9DBG6 (RPN2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
EC=2.4.1.119
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
Ribophorin II
Short name=RPN-II
Ribophorin-2
Gene names
Name:Rpn2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length631 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.

Catalytic activity

Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.

Subunit structure

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the SWP1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 By similarity
Chain23 – 631609Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
PRO_0000022248

Regions

Topological domain23 – 540518Lumenal Potential
Transmembrane541 – 56121Helical; Potential
Transmembrane572 – 59221Helical; Potential
Transmembrane597 – 61721Helical; Potential
Topological domain618 – 63114Cytoplasmic Potential

Amino acid modifications

Glycosylation1061N-linked (GlcNAc...) Potential
Cross-link154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9DBG6 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: D20745D10D5486F9

FASTA63169,063
        10         20         30         40         50         60 
MAPPGSSAVF LLALTITASV QALTPTHYLT KQDVERLKAS LDRPFTDLES AFYSIVGLSS 

        70         80         90        100        110        120 
LGVQVPDVKK ACTFIKSNLD PSNVDSLFYA AQSSQVLSGC EISVSNETKE LLLAAVSEDS 

       130        140        150        160        170        180 
PIAQIYHAVA ALSGFGLPLA SNEALGALTA RLGKEETVLA TVQALQTASH LSQQADLRNI 

       190        200        210        220        230        240 
VEEIEDLVAR LDELGGVYLQ FEEGLELTAL FVAATYKLMD HVGTEPSMKE DQVIQLMNTI 

       250        260        270        280        290        300 
FSKKNFESLS EAFSVASAAA ALSQNRYHVP VVVVPEGSTS DTQEQAILRL QVSNVLSQPL 

       310        320        330        340        350        360 
AQAAVKLEHA KSAATRATVL QKTPFSLVGN VFELNFKNVK LSSGYYDFSV RVEGDSRYIA 

       370        380        390        400        410        420 
NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA KAKGTFIADS HQNFALFFQL 

       430        440        450        460        470        480 
VDVNTGAELT PHQTFVRLHN QKTGQEVVFV AEPDNKNVYK FELDTSERKI EFDSASGTYT 

       490        500        510        520        530        540 
LYLIIGDATL KNPILWNVAD VVIKFPEEEA PSTVLSQSLF TPKQEIQHLF REPEKRPPTV 

       550        560        570        580        590        600 
VSNTFTALIL SPLLLLFALW IRIGANVSNF TFAPSTVIFH LGHAAMLGLM YIYWTQLNMF 

       610        620        630 
QTLKYLAVLG TVTFLAGNRM LAQHAVKRTA H

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Liver.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Colon.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK004968 mRNA. Translation: BAB23707.1.
AK161624 mRNA. Translation: BAE36498.1.
AK166778 mRNA. Translation: BAE39013.1.
AL669828 Genomic DNA. Translation: CAM15974.1.
BC010509 mRNA. Translation: AAH10509.1.
BC046806 mRNA. Translation: AAH46806.1.
IPIIPI00475154.
RefSeqNP_062616.2. NM_019642.4.
UniGeneMm.22130.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ9DBG6. 2 interactions.
STRINGQ9DBG6.

PTM databases

PhosphoSiteQ9DBG6.

Proteomic databases

PRIDEQ9DBG6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000116380; ENSMUSP00000112081; ENSMUSG00000027642.
GeneID20014.
KEGGmmu:20014.
NMPDRfig|10090.3.peg.7300.
UCSCuc008now.1. mouse.

Organism-specific databases

CTD6185.
MGIMGI:98085. Rpn2.

Phylogenomic databases

GeneTreeENSGT00390000002635.
HOVERGENHBG002365.
InParanoidQ9DBG6.
OMATQLNMFQ.
OrthoDBEOG4C87S7.
PhylomeDBQ9DBG6.

Gene expression databases

ArrayExpressQ9DBG6.
BgeeQ9DBG6.
CleanExMM_RPN2.
GenevestigatorQ9DBG6.

Family and domain databases

InterProIPR008814. Ribophorin_II.
[Graphical view]
KOK12667.
PANTHERPTHR12640. Ribophorin_II. 1 hit.
PfamPF05817. Ribophorin_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio297511.
SOURCESearch...

Entry information

Entry nameRPN2_MOUSE
AccessionPrimary (citable) accession number: Q9DBG6
Secondary accession number(s): A2ACG6, Q3TKY0, Q91XH0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2001
Last modified: November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents