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Q9DBG6

- RPN2_MOUSE

UniProt

Q9DBG6 - RPN2_MOUSE

Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene

Rpn2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

    Catalytic activityi

    Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

    Pathwayi

    GO - Molecular functioni

    1. ribosome binding Source: Ensembl
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

    GO - Biological processi

    1. aging Source: Ensembl
    2. protein N-linked glycosylation Source: MGI
    3. response to drug Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_196445. SRP-dependent cotranslational protein targeting to membrane.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (EC:2.4.99.18)
    Alternative name(s):
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
    Ribophorin II
    Short name:
    RPN-II
    Ribophorin-2
    Gene namesi
    Name:Rpn2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:98085. Rpn2.

    Subcellular locationi

    GO - Cellular componenti

    1. autophagic vacuole membrane Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. nucleus Source: Ensembl
    4. oligosaccharyltransferase complex Source: InterPro
    5. rough endoplasmic reticulum Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222By similarityAdd
    BLAST
    Chaini23 – 631609Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2PRO_0000022248Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
    Cross-linki154 – 154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Keywords - PTMi

    Glycoprotein, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiQ9DBG6.
    PaxDbiQ9DBG6.
    PRIDEiQ9DBG6.

    PTM databases

    PhosphoSiteiQ9DBG6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9DBG6.
    BgeeiQ9DBG6.
    CleanExiMM_RPN2.
    GenevestigatoriQ9DBG6.

    Interactioni

    Subunit structurei

    Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202990. 2 interactions.
    IntActiQ9DBG6. 7 interactions.
    MINTiMINT-1859656.
    STRINGi10090.ENSMUSP00000099417.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 540518LumenalSequence AnalysisAdd
    BLAST
    Topological domaini562 – 57110CytoplasmicSequence Analysis
    Topological domaini593 – 5964LumenalSequence Analysis
    Topological domaini618 – 63114CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei541 – 56121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei572 – 59221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei597 – 61721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SWP1 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG258688.
    GeneTreeiENSGT00390000002635.
    HOVERGENiHBG002365.
    InParanoidiA2ACG6.
    KOiK12667.
    OMAiPTHYLTK.
    OrthoDBiEOG78SQHS.
    TreeFamiTF106146.

    Family and domain databases

    InterProiIPR008814. Swp1.
    [Graphical view]
    PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
    PfamiPF05817. Ribophorin_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9DBG6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPPGSSAVF LLALTITASV QALTPTHYLT KQDVERLKAS LDRPFTDLES    50
    AFYSIVGLSS LGVQVPDVKK ACTFIKSNLD PSNVDSLFYA AQSSQVLSGC 100
    EISVSNETKE LLLAAVSEDS PIAQIYHAVA ALSGFGLPLA SNEALGALTA 150
    RLGKEETVLA TVQALQTASH LSQQADLRNI VEEIEDLVAR LDELGGVYLQ 200
    FEEGLELTAL FVAATYKLMD HVGTEPSMKE DQVIQLMNTI FSKKNFESLS 250
    EAFSVASAAA ALSQNRYHVP VVVVPEGSTS DTQEQAILRL QVSNVLSQPL 300
    AQAAVKLEHA KSAATRATVL QKTPFSLVGN VFELNFKNVK LSSGYYDFSV 350
    RVEGDSRYIA NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA 400
    KAKGTFIADS HQNFALFFQL VDVNTGAELT PHQTFVRLHN QKTGQEVVFV 450
    AEPDNKNVYK FELDTSERKI EFDSASGTYT LYLIIGDATL KNPILWNVAD 500
    VVIKFPEEEA PSTVLSQSLF TPKQEIQHLF REPEKRPPTV VSNTFTALIL 550
    SPLLLLFALW IRIGANVSNF TFAPSTVIFH LGHAAMLGLM YIYWTQLNMF 600
    QTLKYLAVLG TVTFLAGNRM LAQHAVKRTA H 631
    Length:631
    Mass (Da):69,063
    Last modified:June 1, 2001 - v1
    Checksum:iD20745D10D5486F9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004968 mRNA. Translation: BAB23707.1.
    AK161624 mRNA. Translation: BAE36498.1.
    AK166778 mRNA. Translation: BAE39013.1.
    AL669828 Genomic DNA. Translation: CAM15974.1.
    BC010509 mRNA. Translation: AAH10509.1.
    BC046806 mRNA. Translation: AAH46806.1.
    CCDSiCCDS16975.1.
    RefSeqiNP_062616.2. NM_019642.4.
    UniGeneiMm.22130.

    Genome annotation databases

    EnsembliENSMUST00000116380; ENSMUSP00000112081; ENSMUSG00000027642.
    GeneIDi20014.
    KEGGimmu:20014.
    UCSCiuc008now.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004968 mRNA. Translation: BAB23707.1 .
    AK161624 mRNA. Translation: BAE36498.1 .
    AK166778 mRNA. Translation: BAE39013.1 .
    AL669828 Genomic DNA. Translation: CAM15974.1 .
    BC010509 mRNA. Translation: AAH10509.1 .
    BC046806 mRNA. Translation: AAH46806.1 .
    CCDSi CCDS16975.1.
    RefSeqi NP_062616.2. NM_019642.4.
    UniGenei Mm.22130.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202990. 2 interactions.
    IntActi Q9DBG6. 7 interactions.
    MINTi MINT-1859656.
    STRINGi 10090.ENSMUSP00000099417.

    PTM databases

    PhosphoSitei Q9DBG6.

    Proteomic databases

    MaxQBi Q9DBG6.
    PaxDbi Q9DBG6.
    PRIDEi Q9DBG6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000116380 ; ENSMUSP00000112081 ; ENSMUSG00000027642 .
    GeneIDi 20014.
    KEGGi mmu:20014.
    UCSCi uc008now.1. mouse.

    Organism-specific databases

    CTDi 6185.
    MGIi MGI:98085. Rpn2.

    Phylogenomic databases

    eggNOGi NOG258688.
    GeneTreei ENSGT00390000002635.
    HOVERGENi HBG002365.
    InParanoidi A2ACG6.
    KOi K12667.
    OMAi PTHYLTK.
    OrthoDBi EOG78SQHS.
    TreeFami TF106146.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196445. SRP-dependent cotranslational protein targeting to membrane.

    Miscellaneous databases

    ChiTaRSi RPN2. mouse.
    NextBioi 297511.
    PROi Q9DBG6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9DBG6.
    Bgeei Q9DBG6.
    CleanExi MM_RPN2.
    Genevestigatori Q9DBG6.

    Family and domain databases

    InterProi IPR008814. Swp1.
    [Graphical view ]
    PANTHERi PTHR12640:SF0. PTHR12640:SF0. 1 hit.
    Pfami PF05817. Ribophorin_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Liver.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Colon.

    Entry informationi

    Entry nameiRPN2_MOUSE
    AccessioniPrimary (citable) accession number: Q9DBG6
    Secondary accession number(s): A2ACG6, Q3TKY0, Q91XH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3