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Q9DBG6

- RPN2_MOUSE

UniProt

Q9DBG6 - RPN2_MOUSE

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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene

Rpn2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi

GO - Molecular functioni

  1. ribosome binding Source: Ensembl
  2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

GO - Biological processi

  1. aging Source: Ensembl
  2. protein N-linked glycosylation Source: MGI
  3. response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_196445. SRP-dependent cotranslational protein targeting to membrane.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (EC:2.4.99.18)
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
Ribophorin II
Short name:
RPN-II
Ribophorin-2
Gene namesi
Name:Rpn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:98085. Rpn2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 540518LumenalSequence AnalysisAdd
BLAST
Transmembranei541 – 56121HelicalSequence AnalysisAdd
BLAST
Topological domaini562 – 57110CytoplasmicSequence Analysis
Transmembranei572 – 59221HelicalSequence AnalysisAdd
BLAST
Topological domaini593 – 5964LumenalSequence Analysis
Transmembranei597 – 61721HelicalSequence AnalysisAdd
BLAST
Topological domaini618 – 63114CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. autophagic vacuole membrane Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleus Source: Ensembl
  4. oligosaccharyltransferase complex Source: InterPro
  5. rough endoplasmic reticulum Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 631609Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2PRO_0000022248Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
Cross-linki154 – 154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ9DBG6.
PaxDbiQ9DBG6.
PRIDEiQ9DBG6.

PTM databases

PhosphoSiteiQ9DBG6.

Expressioni

Gene expression databases

BgeeiQ9DBG6.
CleanExiMM_RPN2.
ExpressionAtlasiQ9DBG6. baseline and differential.
GenevestigatoriQ9DBG6.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi202990. 2 interactions.
IntActiQ9DBG6. 7 interactions.
MINTiMINT-1859656.
STRINGi10090.ENSMUSP00000099417.

Family & Domainsi

Sequence similaritiesi

Belongs to the SWP1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG258688.
GeneTreeiENSGT00390000002635.
HOVERGENiHBG002365.
InParanoidiQ9DBG6.
KOiK12667.
OMAiPTHYLTK.
OrthoDBiEOG78SQHS.
TreeFamiTF106146.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DBG6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPPGSSAVF LLALTITASV QALTPTHYLT KQDVERLKAS LDRPFTDLES
60 70 80 90 100
AFYSIVGLSS LGVQVPDVKK ACTFIKSNLD PSNVDSLFYA AQSSQVLSGC
110 120 130 140 150
EISVSNETKE LLLAAVSEDS PIAQIYHAVA ALSGFGLPLA SNEALGALTA
160 170 180 190 200
RLGKEETVLA TVQALQTASH LSQQADLRNI VEEIEDLVAR LDELGGVYLQ
210 220 230 240 250
FEEGLELTAL FVAATYKLMD HVGTEPSMKE DQVIQLMNTI FSKKNFESLS
260 270 280 290 300
EAFSVASAAA ALSQNRYHVP VVVVPEGSTS DTQEQAILRL QVSNVLSQPL
310 320 330 340 350
AQAAVKLEHA KSAATRATVL QKTPFSLVGN VFELNFKNVK LSSGYYDFSV
360 370 380 390 400
RVEGDSRYIA NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA
410 420 430 440 450
KAKGTFIADS HQNFALFFQL VDVNTGAELT PHQTFVRLHN QKTGQEVVFV
460 470 480 490 500
AEPDNKNVYK FELDTSERKI EFDSASGTYT LYLIIGDATL KNPILWNVAD
510 520 530 540 550
VVIKFPEEEA PSTVLSQSLF TPKQEIQHLF REPEKRPPTV VSNTFTALIL
560 570 580 590 600
SPLLLLFALW IRIGANVSNF TFAPSTVIFH LGHAAMLGLM YIYWTQLNMF
610 620 630
QTLKYLAVLG TVTFLAGNRM LAQHAVKRTA H
Length:631
Mass (Da):69,063
Last modified:June 1, 2001 - v1
Checksum:iD20745D10D5486F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004968 mRNA. Translation: BAB23707.1.
AK161624 mRNA. Translation: BAE36498.1.
AK166778 mRNA. Translation: BAE39013.1.
AL669828 Genomic DNA. Translation: CAM15974.1.
BC010509 mRNA. Translation: AAH10509.1.
BC046806 mRNA. Translation: AAH46806.1.
CCDSiCCDS16975.1.
RefSeqiNP_062616.2. NM_019642.4.
UniGeneiMm.22130.

Genome annotation databases

EnsembliENSMUST00000116380; ENSMUSP00000112081; ENSMUSG00000027642.
GeneIDi20014.
KEGGimmu:20014.
UCSCiuc008now.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004968 mRNA. Translation: BAB23707.1 .
AK161624 mRNA. Translation: BAE36498.1 .
AK166778 mRNA. Translation: BAE39013.1 .
AL669828 Genomic DNA. Translation: CAM15974.1 .
BC010509 mRNA. Translation: AAH10509.1 .
BC046806 mRNA. Translation: AAH46806.1 .
CCDSi CCDS16975.1.
RefSeqi NP_062616.2. NM_019642.4.
UniGenei Mm.22130.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202990. 2 interactions.
IntActi Q9DBG6. 7 interactions.
MINTi MINT-1859656.
STRINGi 10090.ENSMUSP00000099417.

PTM databases

PhosphoSitei Q9DBG6.

Proteomic databases

MaxQBi Q9DBG6.
PaxDbi Q9DBG6.
PRIDEi Q9DBG6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000116380 ; ENSMUSP00000112081 ; ENSMUSG00000027642 .
GeneIDi 20014.
KEGGi mmu:20014.
UCSCi uc008now.1. mouse.

Organism-specific databases

CTDi 6185.
MGIi MGI:98085. Rpn2.

Phylogenomic databases

eggNOGi NOG258688.
GeneTreei ENSGT00390000002635.
HOVERGENi HBG002365.
InParanoidi Q9DBG6.
KOi K12667.
OMAi PTHYLTK.
OrthoDBi EOG78SQHS.
TreeFami TF106146.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_196445. SRP-dependent cotranslational protein targeting to membrane.

Miscellaneous databases

ChiTaRSi Rpn2. mouse.
NextBioi 297511.
PROi Q9DBG6.
SOURCEi Search...

Gene expression databases

Bgeei Q9DBG6.
CleanExi MM_RPN2.
ExpressionAtlasi Q9DBG6. baseline and differential.
Genevestigatori Q9DBG6.

Family and domain databases

InterProi IPR008814. Swp1.
[Graphical view ]
PANTHERi PTHR12640:SF0. PTHR12640:SF0. 1 hit.
Pfami PF05817. Ribophorin_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Colon.

Entry informationi

Entry nameiRPN2_MOUSE
AccessioniPrimary (citable) accession number: Q9DBG6
Secondary accession number(s): A2ACG6, Q3TKY0, Q91XH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3