Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene

Rpn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • protein N-linked glycosylation Source: MGI
  • response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (EC:2.4.99.18)
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
Ribophorin II
Short name:
RPN-II
Ribophorin-2
Gene namesi
Name:Rpn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:98085. Rpn2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 540LumenalSequence analysisAdd BLAST518
Transmembranei541 – 561HelicalSequence analysisAdd BLAST21
Topological domaini562 – 571CytoplasmicSequence analysis10
Transmembranei572 – 592HelicalSequence analysisAdd BLAST21
Topological domaini593 – 596LumenalSequence analysis4
Transmembranei597 – 617HelicalSequence analysisAdd BLAST21
Topological domaini618 – 631CytoplasmicSequence analysisAdd BLAST14

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000002224823 – 631Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2Add BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...)Sequence analysis1
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9DBG6.
MaxQBiQ9DBG6.
PaxDbiQ9DBG6.
PeptideAtlasiQ9DBG6.
PRIDEiQ9DBG6.
TopDownProteomicsiQ9DBG6.

PTM databases

iPTMnetiQ9DBG6.
PhosphoSitePlusiQ9DBG6.
SwissPalmiQ9DBG6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027642.
CleanExiMM_RPN2.
ExpressionAtlasiQ9DBG6. baseline and differential.
GenevisibleiQ9DBG6. MM.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi202990. 3 interactors.
IntActiQ9DBG6. 11 interactors.
MINTiMINT-1859656.
STRINGi10090.ENSMUSP00000112081.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5J6GX-ray3.30E/F373-380[»]
5J6HX-ray2.30F373-380[»]
ProteinModelPortaliQ9DBG6.
SMRiQ9DBG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SWP1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2447. Eukaryota.
ENOG410XRF2. LUCA.
GeneTreeiENSGT00390000002635.
HOVERGENiHBG002365.
InParanoidiQ9DBG6.
KOiK12667.
OMAiVKKACAF.
OrthoDBiEOG091G0H3U.
TreeFamiTF106146.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DBG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPGSSAVF LLALTITASV QALTPTHYLT KQDVERLKAS LDRPFTDLES
60 70 80 90 100
AFYSIVGLSS LGVQVPDVKK ACTFIKSNLD PSNVDSLFYA AQSSQVLSGC
110 120 130 140 150
EISVSNETKE LLLAAVSEDS PIAQIYHAVA ALSGFGLPLA SNEALGALTA
160 170 180 190 200
RLGKEETVLA TVQALQTASH LSQQADLRNI VEEIEDLVAR LDELGGVYLQ
210 220 230 240 250
FEEGLELTAL FVAATYKLMD HVGTEPSMKE DQVIQLMNTI FSKKNFESLS
260 270 280 290 300
EAFSVASAAA ALSQNRYHVP VVVVPEGSTS DTQEQAILRL QVSNVLSQPL
310 320 330 340 350
AQAAVKLEHA KSAATRATVL QKTPFSLVGN VFELNFKNVK LSSGYYDFSV
360 370 380 390 400
RVEGDSRYIA NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA
410 420 430 440 450
KAKGTFIADS HQNFALFFQL VDVNTGAELT PHQTFVRLHN QKTGQEVVFV
460 470 480 490 500
AEPDNKNVYK FELDTSERKI EFDSASGTYT LYLIIGDATL KNPILWNVAD
510 520 530 540 550
VVIKFPEEEA PSTVLSQSLF TPKQEIQHLF REPEKRPPTV VSNTFTALIL
560 570 580 590 600
SPLLLLFALW IRIGANVSNF TFAPSTVIFH LGHAAMLGLM YIYWTQLNMF
610 620 630
QTLKYLAVLG TVTFLAGNRM LAQHAVKRTA H
Length:631
Mass (Da):69,063
Last modified:June 1, 2001 - v1
Checksum:iD20745D10D5486F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004968 mRNA. Translation: BAB23707.1.
AK161624 mRNA. Translation: BAE36498.1.
AK166778 mRNA. Translation: BAE39013.1.
AL669828 Genomic DNA. Translation: CAM15974.1.
BC010509 mRNA. Translation: AAH10509.1.
BC046806 mRNA. Translation: AAH46806.1.
CCDSiCCDS16975.1.
RefSeqiNP_062616.2. NM_019642.4.
UniGeneiMm.22130.

Genome annotation databases

EnsembliENSMUST00000116380; ENSMUSP00000112081; ENSMUSG00000027642.
GeneIDi20014.
KEGGimmu:20014.
UCSCiuc008now.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004968 mRNA. Translation: BAB23707.1.
AK161624 mRNA. Translation: BAE36498.1.
AK166778 mRNA. Translation: BAE39013.1.
AL669828 Genomic DNA. Translation: CAM15974.1.
BC010509 mRNA. Translation: AAH10509.1.
BC046806 mRNA. Translation: AAH46806.1.
CCDSiCCDS16975.1.
RefSeqiNP_062616.2. NM_019642.4.
UniGeneiMm.22130.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5J6GX-ray3.30E/F373-380[»]
5J6HX-ray2.30F373-380[»]
ProteinModelPortaliQ9DBG6.
SMRiQ9DBG6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202990. 3 interactors.
IntActiQ9DBG6. 11 interactors.
MINTiMINT-1859656.
STRINGi10090.ENSMUSP00000112081.

PTM databases

iPTMnetiQ9DBG6.
PhosphoSitePlusiQ9DBG6.
SwissPalmiQ9DBG6.

Proteomic databases

EPDiQ9DBG6.
MaxQBiQ9DBG6.
PaxDbiQ9DBG6.
PeptideAtlasiQ9DBG6.
PRIDEiQ9DBG6.
TopDownProteomicsiQ9DBG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000116380; ENSMUSP00000112081; ENSMUSG00000027642.
GeneIDi20014.
KEGGimmu:20014.
UCSCiuc008now.1. mouse.

Organism-specific databases

CTDi6185.
MGIiMGI:98085. Rpn2.

Phylogenomic databases

eggNOGiKOG2447. Eukaryota.
ENOG410XRF2. LUCA.
GeneTreeiENSGT00390000002635.
HOVERGENiHBG002365.
InParanoidiQ9DBG6.
KOiK12667.
OMAiVKKACAF.
OrthoDBiEOG091G0H3U.
TreeFamiTF106146.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

ChiTaRSiRpn2. mouse.
PROiQ9DBG6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027642.
CleanExiMM_RPN2.
ExpressionAtlasiQ9DBG6. baseline and differential.
GenevisibleiQ9DBG6. MM.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPN2_MOUSE
AccessioniPrimary (citable) accession number: Q9DBG6
Secondary accession number(s): A2ACG6, Q3TKY0, Q91XH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.