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Q9DBF1

- AL7A1_MOUSE

UniProt

Q9DBF1 - AL7A1_MOUSE

Protein

Alpha-aminoadipic semialdehyde dehydrogenase

Gene

Aldh7a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 4 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism By similarity.By similarity

    Catalytic activityi

    (S)-2-amino-6-oxohexanoate + NAD(P)+ + H2O = L-2-aminoadipate + NAD(P)H.
    Betaine aldehyde + NAD+ + H2O = betaine + NADH.
    An aldehyde + NAD+ + H2O = a carboxylate + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei195 – 1951Transition state stabilizerBy similarity
    Active sitei296 – 2961Proton acceptorPROSITE-ProRule annotation
    Active sitei330 – 3301NucleophilePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi274 – 2796NADBy similarity

    GO - Molecular functioni

    1. betaine-aldehyde dehydrogenase activity Source: UniProtKB-EC
    2. L-aminoadipate-semialdehyde dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00529; UER00386.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-aminoadipic semialdehyde dehydrogenase (EC:1.2.1.31)
    Short name:
    Alpha-AASA dehydrogenase
    Alternative name(s):
    Aldehyde dehydrogenase family 7 member A1 (EC:1.2.1.3)
    Antiquitin-1
    Betaine aldehyde dehydrogenase (EC:1.2.1.8)
    Delta1-piperideine-6-carboxylate dehydrogenase
    Short name:
    P6c dehydrogenase
    Gene namesi
    Name:Aldh7a1
    Synonyms:Ald7a1
    OrganismiMus musculus (Mouse)Imported
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:108186. Aldh7a1.

    Subcellular locationi

    Mitochondrion 1 Publication. Nucleus 1 Publication. Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626MitochondrionSequence AnalysisAdd
    BLAST
    Chaini27 – 539513Alpha-aminoadipic semialdehyde dehydrogenasePRO_0000056491Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei86 – 861N6-acetyllysine; alternate1 Publication
    Modified residuei86 – 861N6-succinyllysine; alternate1 Publication
    Modified residuei94 – 941N6-acetyllysine; alternate1 Publication
    Modified residuei94 – 941N6-succinyllysine; alternate1 Publication
    Modified residuei97 – 971N6-acetyllysine; alternate1 Publication
    Modified residuei97 – 971N6-succinyllysine; alternate1 Publication
    Modified residuei462 – 4621N6-acetyllysine1 Publication
    Modified residuei500 – 5001N6-acetyllysine1 Publication
    Modified residuei537 – 5371N6-succinyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9DBF1.
    PaxDbiQ9DBF1.
    PRIDEiQ9DBF1.

    2D gel databases

    REPRODUCTION-2DPAGEQ9DBF1.

    PTM databases

    PhosphoSiteiQ9DBF1.

    Expressioni

    Tissue specificityi

    Present in liver, kidney, brain and pancreas, and at lower levels in jejunum, duodenum, stomach and testes (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiQ9DBF1.
    BgeeiQ9DBF1.
    CleanExiMM_ALDH7A1.
    GenevestigatoriQ9DBF1.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    IntActiQ9DBF1. 4 interactions.
    MINTiMINT-1859388.
    STRINGi10090.ENSMUSP00000112334.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DBF1.
    SMRiQ9DBF1. Positions 30-527.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1012.
    GeneTreeiENSGT00720000108597.
    HOGENOMiHOG000271511.
    HOVERGENiHBG050485.
    InParanoidiQ3UKT6.
    KOiK14085.
    OMAiNAIIVFE.
    OrthoDBiEOG78D7JV.
    PhylomeDBiQ9DBF1.
    TreeFamiTF300388.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    PROSITEiPS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9DBF1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MWRVPRRLCV QSVKTSKLSG PWSRPAAHMS TLLIHHPQYA WLQDLGLRED    50
    NEGVYNGSWG GRGEVITTYC PANNEPIARV RQASLKDYEE TIGKAKKAWN 100
    IWADIPAPKR GEIVRKIGDA FREKIQLLGR LVSLEMGKIL VEGIGEVQEY 150
    VDVCDYAAGL SRMIGGPTLP SERPGHALIE MWNPLGLVGI ITAFNFPVAV 200
    FGWNNAIALI TGNVCLWKGA PTTSLVSVAV TKIIAQVLED NLLPGAICSL 250
    VCGGADIGTT MARDERVNLL SFTGSTQVGK EVALMVQERF GKSLLELGGN 300
    NAIIAFEDAD LSLVVPSVLF AAVGTAGQRC TTVRRLFLHE SIHNEVVDRL 350
    RSAYSQIRVG NPWDPNILYG PLHTKQAVSM FVRAVEEAKK QGGTVVYGGK 400
    VMDHPGNYVE PTIVTGLAHD APIVHQETFA PILYVFKFQD EEEVFEWNNE 450
    VKQGLSSSIF TKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK 500
    HTGGGRESGS DAWKQYMRRS TCTINYSTSL PLAQGIKFQ 539
    Length:539
    Mass (Da):58,861
    Last modified:April 20, 2010 - v4
    Checksum:i7591EE5652F2577E
    GO
    Isoform 2 (identifier: Q9DBF1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-28: Missing.

    Show »
    Length:511
    Mass (Da):55,645
    Checksum:i37952CEC7C5F3A85
    GO

    Sequence cautioni

    The sequence AAH12407.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti164 – 1641I → M in BAE26715. (PubMed:16141072)Curated
    Sequence conflicti467 – 4671I → V in BAE35641. (PubMed:16141072)Curated
    Sequence conflicti491 – 4911E → G in BAE35641. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2828Missing in isoform 2. 1 PublicationVSP_038989Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004991 mRNA. Translation: BAB23726.1.
    AK145873 mRNA. Translation: BAE26715.1.
    AK160117 mRNA. Translation: BAE35641.1.
    AK169195 mRNA. Translation: BAE40971.1.
    BC012407 mRNA. Translation: AAH12407.1. Different initiation.
    CCDSiCCDS29258.2. [Q9DBF1-1]
    CCDS50291.1. [Q9DBF1-2]
    RefSeqiNP_001120810.1. NM_001127338.1. [Q9DBF1-2]
    NP_613066.2. NM_138600.4. [Q9DBF1-1]
    UniGeneiMm.30250.

    Genome annotation databases

    EnsembliENSMUST00000066208; ENSMUSP00000065089; ENSMUSG00000053644. [Q9DBF1-1]
    ENSMUST00000174518; ENSMUSP00000133372; ENSMUSG00000053644. [Q9DBF1-2]
    GeneIDi110695.
    KEGGimmu:110695.
    UCSCiuc008eyn.2. mouse. [Q9DBF1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004991 mRNA. Translation: BAB23726.1 .
    AK145873 mRNA. Translation: BAE26715.1 .
    AK160117 mRNA. Translation: BAE35641.1 .
    AK169195 mRNA. Translation: BAE40971.1 .
    BC012407 mRNA. Translation: AAH12407.1 . Different initiation.
    CCDSi CCDS29258.2. [Q9DBF1-1 ]
    CCDS50291.1. [Q9DBF1-2 ]
    RefSeqi NP_001120810.1. NM_001127338.1. [Q9DBF1-2 ]
    NP_613066.2. NM_138600.4. [Q9DBF1-1 ]
    UniGenei Mm.30250.

    3D structure databases

    ProteinModelPortali Q9DBF1.
    SMRi Q9DBF1. Positions 30-527.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9DBF1. 4 interactions.
    MINTi MINT-1859388.
    STRINGi 10090.ENSMUSP00000112334.

    PTM databases

    PhosphoSitei Q9DBF1.

    2D gel databases

    REPRODUCTION-2DPAGE Q9DBF1.

    Proteomic databases

    MaxQBi Q9DBF1.
    PaxDbi Q9DBF1.
    PRIDEi Q9DBF1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000066208 ; ENSMUSP00000065089 ; ENSMUSG00000053644 . [Q9DBF1-1 ]
    ENSMUST00000174518 ; ENSMUSP00000133372 ; ENSMUSG00000053644 . [Q9DBF1-2 ]
    GeneIDi 110695.
    KEGGi mmu:110695.
    UCSCi uc008eyn.2. mouse. [Q9DBF1-1 ]

    Organism-specific databases

    CTDi 501.
    MGIi MGI:108186. Aldh7a1.

    Phylogenomic databases

    eggNOGi COG1012.
    GeneTreei ENSGT00720000108597.
    HOGENOMi HOG000271511.
    HOVERGENi HBG050485.
    InParanoidi Q3UKT6.
    KOi K14085.
    OMAi NAIIVFE.
    OrthoDBi EOG78D7JV.
    PhylomeDBi Q9DBF1.
    TreeFami TF300388.

    Enzyme and pathway databases

    UniPathwayi UPA00529 ; UER00386 .

    Miscellaneous databases

    NextBioi 364487.
    PROi Q9DBF1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9DBF1.
    Bgeei Q9DBF1.
    CleanExi MM_ALDH7A1.
    Genevestigatori Q9DBF1.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    PROSITEi PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Salivary gland.
    3. "Aldehyde dehydrogenase 7A1 (ALDH7A1) is a novel enzyme involved in cellular defense against hyperosmotic stress."
      Brocker C., Lassen N., Estey T., Pappa A., Cantore M., Orlova V.V., Chavakis T., Kavanagh K.L., Oppermann U., Vasiliou V.
      J. Biol. Chem. 285:18452-18463(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-86; LYS-94; LYS-97 AND LYS-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-86; LYS-94; LYS-97; LYS-462 AND LYS-500, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiAL7A1_MOUSE
    AccessioniPrimary (citable) accession number: Q9DBF1
    Secondary accession number(s): Q3TFC7, Q3TVH7, Q3UKT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 117 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3