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Protein

Alpha-1,3/1,6-mannosyltransferase ALG2

Gene

Alg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mannosylates Man2GlcNAc(2)-dolichol diphosphate and Man1GlcNAc(2)-dolichol diphosphate to form Man3GlcNAc(2)-dolichol diphosphate.By similarity

Catalytic activityi

GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol.
GDP-D-mannose + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3/1,6-mannosyltransferase ALG2 (EC:2.4.1.132, EC:2.4.1.257)
Alternative name(s):
Asparagine-linked glycosylation protein 2 homolog
GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Gene namesi
Name:Alg2
ORF Names:MNCb-5081
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914731. Alg2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei85 – 105HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802611 – 415Alpha-1,3/1,6-mannosyltransferase ALG2Add BLAST415

Proteomic databases

EPDiQ9DBE8.
MaxQBiQ9DBE8.
PaxDbiQ9DBE8.
PeptideAtlasiQ9DBE8.
PRIDEiQ9DBE8.

PTM databases

iPTMnetiQ9DBE8.
PhosphoSitePlusiQ9DBE8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039740.
ExpressionAtlasiQ9DBE8. baseline and differential.
GenevisibleiQ9DBE8. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9DBE8. 1 interactor.
MINTiMINT-4998111.
STRINGi10090.ENSMUSP00000043580.

Structurei

3D structure databases

ProteinModelPortaliQ9DBE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0853. Eukaryota.
COG0438. LUCA.
GeneTreeiENSGT00550000075033.
HOGENOMiHOG000177048.
HOVERGENiHBG009445.
InParanoidiQ9DBE8.
KOiK03843.
OMAiQIWTAHY.
OrthoDBiEOG091G0AE2.
PhylomeDBiQ9DBE8.
TreeFamiTF106000.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DBE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENLYRARS RVYSPSVLFL HPDMGIGGAE RLVLDAALAL QEYGCDVKIW
60 70 80 90 100
TAHYDPNHCF IETRELSVQC AGDWLPRSLG WGGRGAAICS YVRMVFLALY
110 120 130 140 150
VLFLSGEEFD VVVCDQVSAC IPVFKLARRR KRVLFYCHFP DLLLTQRNSA
160 170 180 190 200
LKKFYRAPID WIEEYTTGMA DRILVNSQYT ASVFKETFKT LSHRNPDVLY
210 220 230 240 250
PSLNIGSFDL AIPEKIDDLV PKGKQFLFLS INRYERKKNL PLALRSLVQL
260 270 280 290 300
RNRLPSQEWD KVHLFMAGGY DDRIPENVEH YKELKKMVQE SDLERHVTFL
310 320 330 340 350
RSFSDRQKIS LLHGCLCVLY TPSNEHFGIV PLEAMYMQCP VIAVNNGGPL
360 370 380 390 400
ESIVHKVTGF LCEPDPVHFS EAMEKFIHKP SLKATMGLAG KARVAEKFSA
410
DAFADQLYQY VTKLV
Length:415
Mass (Da):47,405
Last modified:April 13, 2004 - v2
Checksum:i7462CA9DEF74BA13
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti296H → Y in BAB27106 (PubMed:16141072).Curated1
Sequence conflicti376F → L in BAB23731 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB161357 mRNA. Translation: BAD11906.1.
AB041604 mRNA. Translation: BAA95087.1.
AK004997 mRNA. Translation: BAB23731.1.
AK010673 mRNA. Translation: BAB27106.1.
AL772150 Genomic DNA. Translation: CAM24920.1.
BC051951 mRNA. Translation: AAH51951.1.
BC052411 mRNA. Translation: AAH52411.2.
CCDSiCCDS18161.1.
RefSeqiNP_064382.3. NM_019998.3.
UniGeneiMm.22218.

Genome annotation databases

EnsembliENSMUST00000044148; ENSMUSP00000043580; ENSMUSG00000039740.
GeneIDi56737.
KEGGimmu:56737.
UCSCiuc008suq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB161357 mRNA. Translation: BAD11906.1.
AB041604 mRNA. Translation: BAA95087.1.
AK004997 mRNA. Translation: BAB23731.1.
AK010673 mRNA. Translation: BAB27106.1.
AL772150 Genomic DNA. Translation: CAM24920.1.
BC051951 mRNA. Translation: AAH51951.1.
BC052411 mRNA. Translation: AAH52411.2.
CCDSiCCDS18161.1.
RefSeqiNP_064382.3. NM_019998.3.
UniGeneiMm.22218.

3D structure databases

ProteinModelPortaliQ9DBE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DBE8. 1 interactor.
MINTiMINT-4998111.
STRINGi10090.ENSMUSP00000043580.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

PTM databases

iPTMnetiQ9DBE8.
PhosphoSitePlusiQ9DBE8.

Proteomic databases

EPDiQ9DBE8.
MaxQBiQ9DBE8.
PaxDbiQ9DBE8.
PeptideAtlasiQ9DBE8.
PRIDEiQ9DBE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044148; ENSMUSP00000043580; ENSMUSG00000039740.
GeneIDi56737.
KEGGimmu:56737.
UCSCiuc008suq.2. mouse.

Organism-specific databases

CTDi85365.
MGIiMGI:1914731. Alg2.

Phylogenomic databases

eggNOGiKOG0853. Eukaryota.
COG0438. LUCA.
GeneTreeiENSGT00550000075033.
HOGENOMiHOG000177048.
HOVERGENiHBG009445.
InParanoidiQ9DBE8.
KOiK03843.
OMAiQIWTAHY.
OrthoDBiEOG091G0AE2.
PhylomeDBiQ9DBE8.
TreeFamiTF106000.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ9DBE8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039740.
ExpressionAtlasiQ9DBE8. baseline and differential.
GenevisibleiQ9DBE8. MM.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG2_MOUSE
AccessioniPrimary (citable) accession number: Q9DBE8
Secondary accession number(s): Q7TN30, Q9CWI6, Q9JJA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.