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Q9DBE8

- ALG2_MOUSE

UniProt

Q9DBE8 - ALG2_MOUSE

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Protein

Alpha-1,3/1,6-mannosyltransferase ALG2

Gene

Alg2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Mannosylates Man2GlcNAc(2)-dolichol diphosphate and Man1GlcNAc(2)-dolichol diphosphate to form Man3GlcNAc(2)-dolichol diphosphate.By similarity

Catalytic activityi

GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol.
GDP-D-mannose + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.

Pathwayi

GO - Molecular functioni

  1. alpha-1,3-mannosyltransferase activity Source: Ensembl
  2. GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity Source: UniProtKB-EC
  3. glycolipid 6-alpha-mannosyltransferase activity Source: InterPro

GO - Biological processi

  1. dolichol-linked oligosaccharide biosynthetic process Source: Ensembl
  2. protein glycosylation in endoplasmic reticulum Source: Ensembl
  3. response to calcium ion Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_262413. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3/1,6-mannosyltransferase ALG2 (EC:2.4.1.132, EC:2.4.1.257)
Alternative name(s):
Asparagine-linked glycosylation protein 2 homolog
GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Gene namesi
Name:Alg2
ORF Names:MNCb-5081
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1914731. Alg2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei85 – 10521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. nucleus Source: Ensembl
  3. perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Alpha-1,3/1,6-mannosyltransferase ALG2PRO_0000080261Add
BLAST

Proteomic databases

MaxQBiQ9DBE8.
PaxDbiQ9DBE8.
PRIDEiQ9DBE8.

PTM databases

PhosphoSiteiQ9DBE8.

Expressioni

Gene expression databases

BgeeiQ9DBE8.
GenevestigatoriQ9DBE8.

Interactioni

Protein-protein interaction databases

BioGridi208151. 3 interactions.
IntActiQ9DBE8. 1 interaction.
MINTiMINT-4998111.

Structurei

3D structure databases

ProteinModelPortaliQ9DBE8.
SMRiQ9DBE8. Positions 153-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00550000075033.
HOGENOMiHOG000177048.
HOVERGENiHBG009445.
InParanoidiQ9DBE8.
KOiK03843.
OMAiGETGWLR.
OrthoDBiEOG7HTHH4.
PhylomeDBiQ9DBE8.
TreeFamiTF106000.

Family and domain databases

InterProiIPR027054. ALG2.
IPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4_N.
[Graphical view]
PANTHERiPTHR12526:SF221. PTHR12526:SF221. 1 hit.
PfamiPF13579. Glyco_trans_4_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DBE8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAENLYRARS RVYSPSVLFL HPDMGIGGAE RLVLDAALAL QEYGCDVKIW
60 70 80 90 100
TAHYDPNHCF IETRELSVQC AGDWLPRSLG WGGRGAAICS YVRMVFLALY
110 120 130 140 150
VLFLSGEEFD VVVCDQVSAC IPVFKLARRR KRVLFYCHFP DLLLTQRNSA
160 170 180 190 200
LKKFYRAPID WIEEYTTGMA DRILVNSQYT ASVFKETFKT LSHRNPDVLY
210 220 230 240 250
PSLNIGSFDL AIPEKIDDLV PKGKQFLFLS INRYERKKNL PLALRSLVQL
260 270 280 290 300
RNRLPSQEWD KVHLFMAGGY DDRIPENVEH YKELKKMVQE SDLERHVTFL
310 320 330 340 350
RSFSDRQKIS LLHGCLCVLY TPSNEHFGIV PLEAMYMQCP VIAVNNGGPL
360 370 380 390 400
ESIVHKVTGF LCEPDPVHFS EAMEKFIHKP SLKATMGLAG KARVAEKFSA
410
DAFADQLYQY VTKLV
Length:415
Mass (Da):47,405
Last modified:April 13, 2004 - v2
Checksum:i7462CA9DEF74BA13
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti296 – 2961H → Y in BAB27106. (PubMed:16141072)Curated
Sequence conflicti376 – 3761F → L in BAB23731. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB161357 mRNA. Translation: BAD11906.1.
AB041604 mRNA. Translation: BAA95087.1.
AK004997 mRNA. Translation: BAB23731.1.
AK010673 mRNA. Translation: BAB27106.1.
AL772150 Genomic DNA. Translation: CAM24920.1.
BC051951 mRNA. Translation: AAH51951.1.
BC052411 mRNA. Translation: AAH52411.2.
CCDSiCCDS18161.1.
RefSeqiNP_064382.3. NM_019998.3.
UniGeneiMm.22218.

Genome annotation databases

EnsembliENSMUST00000044148; ENSMUSP00000043580; ENSMUSG00000039740.
GeneIDi56737.
KEGGimmu:56737.
UCSCiuc008suq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB161357 mRNA. Translation: BAD11906.1 .
AB041604 mRNA. Translation: BAA95087.1 .
AK004997 mRNA. Translation: BAB23731.1 .
AK010673 mRNA. Translation: BAB27106.1 .
AL772150 Genomic DNA. Translation: CAM24920.1 .
BC051951 mRNA. Translation: AAH51951.1 .
BC052411 mRNA. Translation: AAH52411.2 .
CCDSi CCDS18161.1.
RefSeqi NP_064382.3. NM_019998.3.
UniGenei Mm.22218.

3D structure databases

ProteinModelPortali Q9DBE8.
SMRi Q9DBE8. Positions 153-407.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208151. 3 interactions.
IntActi Q9DBE8. 1 interaction.
MINTi MINT-4998111.

Protein family/group databases

CAZyi GT4. Glycosyltransferase Family 4.

PTM databases

PhosphoSitei Q9DBE8.

Proteomic databases

MaxQBi Q9DBE8.
PaxDbi Q9DBE8.
PRIDEi Q9DBE8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000044148 ; ENSMUSP00000043580 ; ENSMUSG00000039740 .
GeneIDi 56737.
KEGGi mmu:56737.
UCSCi uc008suq.2. mouse.

Organism-specific databases

CTDi 85365.
MGIi MGI:1914731. Alg2.

Phylogenomic databases

eggNOGi COG0438.
GeneTreei ENSGT00550000075033.
HOGENOMi HOG000177048.
HOVERGENi HBG009445.
InParanoidi Q9DBE8.
KOi K03843.
OMAi GETGWLR.
OrthoDBi EOG7HTHH4.
PhylomeDBi Q9DBE8.
TreeFami TF106000.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_262413. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

NextBioi 313228.
PROi Q9DBE8.
SOURCEi Search...

Gene expression databases

Bgeei Q9DBE8.
Genevestigatori Q9DBE8.

Family and domain databases

InterProi IPR027054. ALG2.
IPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4_N.
[Graphical view ]
PANTHERi PTHR12526:SF221. PTHR12526:SF221. 1 hit.
Pfami PF13579. Glyco_trans_4_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the mammalian genes which complement the defect of the yeast alg2 mutation."
    Takahashi T., Ueda M., Itoh N., Okutomi S., Nishikawa Y.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method."
    Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell and Liver.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Embryonic brain.

Entry informationi

Entry nameiALG2_MOUSE
AccessioniPrimary (citable) accession number: Q9DBE8
Secondary accession number(s): Q7TN30, Q9CWI6, Q9JJA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 26, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3