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Q9DBE8

- ALG2_MOUSE

UniProt

Q9DBE8 - ALG2_MOUSE

Protein

Alpha-1,3/1,6-mannosyltransferase ALG2

Gene

Alg2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (13 Apr 2004)
      Previous versions | rss
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    Functioni

    Mannosylates Man2GlcNAc(2)-dolichol diphosphate and Man1GlcNAc(2)-dolichol diphosphate to form Man3GlcNAc(2)-dolichol diphosphate.By similarity

    Catalytic activityi

    GDP-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol.
    GDP-D-mannose + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = GDP + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.

    Pathwayi

    GO - Molecular functioni

    1. alpha-1,3-mannosyltransferase activity Source: Ensembl
    2. GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity Source: UniProtKB-EC
    3. glycolipid 6-alpha-mannosyltransferase activity Source: InterPro

    GO - Biological processi

    1. dolichol-linked oligosaccharide biosynthetic process Source: Ensembl
    2. protein glycosylation in endoplasmic reticulum Source: Ensembl
    3. response to calcium ion Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-1,3/1,6-mannosyltransferase ALG2 (EC:2.4.1.132, EC:2.4.1.257)
    Alternative name(s):
    Asparagine-linked glycosylation protein 2 homolog
    GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
    GDP-Man:Man(1)GlcNAc(2)-PP-dolichol mannosyltransferase
    GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
    Gene namesi
    Name:Alg2
    ORF Names:MNCb-5081
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1914731. Alg2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. nucleus Source: Ensembl
    3. perinuclear region of cytoplasm Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415Alpha-1,3/1,6-mannosyltransferase ALG2PRO_0000080261Add
    BLAST

    Proteomic databases

    MaxQBiQ9DBE8.
    PaxDbiQ9DBE8.
    PRIDEiQ9DBE8.

    PTM databases

    PhosphoSiteiQ9DBE8.

    Expressioni

    Gene expression databases

    BgeeiQ9DBE8.
    GenevestigatoriQ9DBE8.

    Interactioni

    Protein-protein interaction databases

    BioGridi208151. 3 interactions.
    IntActiQ9DBE8. 1 interaction.
    MINTiMINT-4998111.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DBE8.
    SMRiQ9DBE8. Positions 153-407.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei85 – 10521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0438.
    GeneTreeiENSGT00550000075033.
    HOGENOMiHOG000177048.
    HOVERGENiHBG009445.
    InParanoidiQ9DBE8.
    KOiK03843.
    OMAiGETGWLR.
    OrthoDBiEOG7HTHH4.
    PhylomeDBiQ9DBE8.
    TreeFamiTF106000.

    Family and domain databases

    InterProiIPR027054. ALG2.
    IPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4_N.
    [Graphical view]
    PANTHERiPTHR12526:SF221. PTHR12526:SF221. 1 hit.
    PfamiPF13579. Glyco_trans_4_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9DBE8-1 [UniParc]FASTAAdd to Basket

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    MAENLYRARS RVYSPSVLFL HPDMGIGGAE RLVLDAALAL QEYGCDVKIW    50
    TAHYDPNHCF IETRELSVQC AGDWLPRSLG WGGRGAAICS YVRMVFLALY 100
    VLFLSGEEFD VVVCDQVSAC IPVFKLARRR KRVLFYCHFP DLLLTQRNSA 150
    LKKFYRAPID WIEEYTTGMA DRILVNSQYT ASVFKETFKT LSHRNPDVLY 200
    PSLNIGSFDL AIPEKIDDLV PKGKQFLFLS INRYERKKNL PLALRSLVQL 250
    RNRLPSQEWD KVHLFMAGGY DDRIPENVEH YKELKKMVQE SDLERHVTFL 300
    RSFSDRQKIS LLHGCLCVLY TPSNEHFGIV PLEAMYMQCP VIAVNNGGPL 350
    ESIVHKVTGF LCEPDPVHFS EAMEKFIHKP SLKATMGLAG KARVAEKFSA 400
    DAFADQLYQY VTKLV 415
    Length:415
    Mass (Da):47,405
    Last modified:April 13, 2004 - v2
    Checksum:i7462CA9DEF74BA13
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti296 – 2961H → Y in BAB27106. (PubMed:16141072)Curated
    Sequence conflicti376 – 3761F → L in BAB23731. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB161357 mRNA. Translation: BAD11906.1.
    AB041604 mRNA. Translation: BAA95087.1.
    AK004997 mRNA. Translation: BAB23731.1.
    AK010673 mRNA. Translation: BAB27106.1.
    AL772150 Genomic DNA. Translation: CAM24920.1.
    BC051951 mRNA. Translation: AAH51951.1.
    BC052411 mRNA. Translation: AAH52411.2.
    CCDSiCCDS18161.1.
    RefSeqiNP_064382.3. NM_019998.3.
    UniGeneiMm.22218.

    Genome annotation databases

    EnsembliENSMUST00000044148; ENSMUSP00000043580; ENSMUSG00000039740.
    GeneIDi56737.
    KEGGimmu:56737.
    UCSCiuc008suq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB161357 mRNA. Translation: BAD11906.1 .
    AB041604 mRNA. Translation: BAA95087.1 .
    AK004997 mRNA. Translation: BAB23731.1 .
    AK010673 mRNA. Translation: BAB27106.1 .
    AL772150 Genomic DNA. Translation: CAM24920.1 .
    BC051951 mRNA. Translation: AAH51951.1 .
    BC052411 mRNA. Translation: AAH52411.2 .
    CCDSi CCDS18161.1.
    RefSeqi NP_064382.3. NM_019998.3.
    UniGenei Mm.22218.

    3D structure databases

    ProteinModelPortali Q9DBE8.
    SMRi Q9DBE8. Positions 153-407.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208151. 3 interactions.
    IntActi Q9DBE8. 1 interaction.
    MINTi MINT-4998111.

    Protein family/group databases

    CAZyi GT4. Glycosyltransferase Family 4.

    PTM databases

    PhosphoSitei Q9DBE8.

    Proteomic databases

    MaxQBi Q9DBE8.
    PaxDbi Q9DBE8.
    PRIDEi Q9DBE8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000044148 ; ENSMUSP00000043580 ; ENSMUSG00000039740 .
    GeneIDi 56737.
    KEGGi mmu:56737.
    UCSCi uc008suq.2. mouse.

    Organism-specific databases

    CTDi 85365.
    MGIi MGI:1914731. Alg2.

    Phylogenomic databases

    eggNOGi COG0438.
    GeneTreei ENSGT00550000075033.
    HOGENOMi HOG000177048.
    HOVERGENi HBG009445.
    InParanoidi Q9DBE8.
    KOi K03843.
    OMAi GETGWLR.
    OrthoDBi EOG7HTHH4.
    PhylomeDBi Q9DBE8.
    TreeFami TF106000.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 313228.
    PROi Q9DBE8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9DBE8.
    Genevestigatori Q9DBE8.

    Family and domain databases

    InterProi IPR027054. ALG2.
    IPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4_N.
    [Graphical view ]
    PANTHERi PTHR12526:SF221. PTHR12526:SF221. 1 hit.
    Pfami PF13579. Glyco_trans_4_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the mammalian genes which complement the defect of the yeast alg2 mutation."
      Takahashi T., Ueda M., Itoh N., Okutomi S., Nishikawa Y.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method."
      Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryonic stem cell and Liver.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Embryonic brain.

    Entry informationi

    Entry nameiALG2_MOUSE
    AccessioniPrimary (citable) accession number: Q9DBE8
    Secondary accession number(s): Q7TN30, Q9CWI6, Q9JJA8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: April 13, 2004
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3