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Protein

E3 ubiquitin-protein ligase MARCH8

Gene

March8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS (By similarity).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri68 – 129RING-CH-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

  • MHC class II protein binding Source: BHF-UCL
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • antigen processing and presentation of peptide antigen via MHC class II Source: UniProtKB
  • negative regulation of MHC class II biosynthetic process Source: BHF-UCL
  • protein polyubiquitination Source: MGI
  • protein ubiquitination Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processAdaptive immunity, Immunity, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH8 (EC:2.3.2.27)
Alternative name(s):
Cellular modulator of immune recognition
Short name:
c-MIR
Membrane-associated RING finger protein 8
Membrane-associated RING-CH protein VIII
Short name:
MARCH-VIII
RING-type E3 ubiquitin transferase MARCH8Curated
Gene namesi
Name:March8
Synonyms:Mir
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1919029. March8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002743711 – 286E3 ubiquitin-protein ligase MARCH8Add BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei249PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9DBD2.
PaxDbiQ9DBD2.
PRIDEiQ9DBD2.

PTM databases

iPTMnetiQ9DBD2.
PhosphoSitePlusiQ9DBD2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025702.
CleanExiMM_MARCH8.
ExpressionAtlasiQ9DBD2. baseline and differential.
GenevisibleiQ9DBD2. MM.

Interactioni

Subunit structurei

Interacts with CD86.By similarity

GO - Molecular functioni

  • MHC class II protein binding Source: BHF-UCL

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078024.

Structurei

3D structure databases

ProteinModelPortaliQ9DBD2.
SMRiQ9DBD2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri68 – 129RING-CH-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110355.
HOGENOMiHOG000113483.
HOVERGENiHBG081957.
InParanoidiQ9DBD2.
KOiK10656.
OrthoDBiEOG091G0WJX.
PhylomeDBiQ9DBD2.
TreeFamiTF319557.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiView protein in Pfam
PF12906. RINGv. 1 hit.
SMARTiView protein in SMART
SM00744. RINGv. 1 hit.
PROSITEiView protein in PROSITE
PS51292. ZF_RING_CH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DBD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMPLHQISA IPSQDAISAR VYRSKTKDKE QNEKTLGHSM SHPSNISKAG
60 70 80 90 100
SSPPSTTAPV SAFSRTSVTP SNQDICRICH CEGDDESPLI TPCHCTGSLH
110 120 130 140 150
FVHQACLQQW IKSSDTRCCE LCKYEFIMET KLKPLRKWEK LQMTASERRK
160 170 180 190 200
IMCSVTFHVI AITCVVWSLY VLIDRTAEEI KQGQVTGILE WPFWTKLVVV
210 220 230 240 250
AIGFTGGLLF MYVQCKVYLQ LWKRLKAYNR VIYVQNCPET SKKNIFEKSA
260 270 280
LTEPTLENKE GHGMCHSTTN SSCTEPEDTG AEIINV
Length:286
Mass (Da):32,243
Last modified:June 1, 2001 - v1
Checksum:i3F4D68112A4C0400
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005032 mRNA. Translation: BAB23759.1.
AK154976 mRNA. Translation: BAE32966.1.
BC050908 mRNA. Translation: AAH50908.1.
BC053090 mRNA. Translation: AAH53090.1.
CCDSiCCDS20451.1.
RefSeqiNP_001289312.1. NM_001302383.1.
NP_001289313.1. NM_001302384.1.
NP_001289314.1. NM_001302385.1.
NP_082196.1. NM_027920.5.
UniGeneiMm.27064.

Genome annotation databases

EnsembliENSMUST00000079012; ENSMUSP00000078024; ENSMUSG00000025702.
ENSMUST00000101032; ENSMUSP00000098594; ENSMUSG00000025702.
GeneIDi71779.
KEGGimmu:71779.
UCSCiuc009djz.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMARH8_MOUSE
AccessioniPrimary (citable) accession number: Q9DBD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: June 1, 2001
Last modified: May 10, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways