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Protein

E3 ubiquitin-protein ligase MARCH8

Gene

March8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS (By similarity).By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri68 – 12962RING-CH-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • MHC class II protein binding Source: BHF-UCL
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • antigen processing and presentation of peptide antigen via MHC class II Source: UniProtKB
  • negative regulation of MHC class II biosynthetic process Source: BHF-UCL
  • protein polyubiquitination Source: MGI
  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Adaptive immunity, Immunity, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH8 (EC:6.3.2.-)
Alternative name(s):
Cellular modulator of immune recognition
Short name:
c-MIR
Membrane-associated RING finger protein 8
Membrane-associated RING-CH protein VIII
Short name:
MARCH-VIII
Gene namesi
Name:March8
Synonyms:Mir
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1919029. March8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286E3 ubiquitin-protein ligase MARCH8PRO_0000274371Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei249 – 2491PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9DBD2.
PaxDbiQ9DBD2.
PRIDEiQ9DBD2.

PTM databases

iPTMnetiQ9DBD2.
PhosphoSiteiQ9DBD2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025702.
CleanExiMM_MARCH8.
ExpressionAtlasiQ9DBD2. baseline and differential.
GenevisibleiQ9DBD2. MM.

Interactioni

Subunit structurei

Interacts with CD86.By similarity

GO - Molecular functioni

  • MHC class II protein binding Source: BHF-UCL

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078024.

Structurei

3D structure databases

ProteinModelPortaliQ9DBD2.
SMRiQ9DBD2. Positions 68-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Sequence similaritiesi

Contains 1 RING-CH-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri68 – 12962RING-CH-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110355.
HOGENOMiHOG000113483.
HOVERGENiHBG081957.
InParanoidiQ9DBD2.
KOiK10656.
OrthoDBiEOG091G0WJX.
PhylomeDBiQ9DBD2.
TreeFamiTF319557.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DBD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMPLHQISA IPSQDAISAR VYRSKTKDKE QNEKTLGHSM SHPSNISKAG
60 70 80 90 100
SSPPSTTAPV SAFSRTSVTP SNQDICRICH CEGDDESPLI TPCHCTGSLH
110 120 130 140 150
FVHQACLQQW IKSSDTRCCE LCKYEFIMET KLKPLRKWEK LQMTASERRK
160 170 180 190 200
IMCSVTFHVI AITCVVWSLY VLIDRTAEEI KQGQVTGILE WPFWTKLVVV
210 220 230 240 250
AIGFTGGLLF MYVQCKVYLQ LWKRLKAYNR VIYVQNCPET SKKNIFEKSA
260 270 280
LTEPTLENKE GHGMCHSTTN SSCTEPEDTG AEIINV
Length:286
Mass (Da):32,243
Last modified:June 1, 2001 - v1
Checksum:i3F4D68112A4C0400
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005032 mRNA. Translation: BAB23759.1.
AK154976 mRNA. Translation: BAE32966.1.
BC050908 mRNA. Translation: AAH50908.1.
BC053090 mRNA. Translation: AAH53090.1.
CCDSiCCDS20451.1.
RefSeqiNP_001289312.1. NM_001302383.1.
NP_001289313.1. NM_001302384.1.
NP_001289314.1. NM_001302385.1.
NP_082196.1. NM_027920.5.
UniGeneiMm.27064.

Genome annotation databases

EnsembliENSMUST00000079012; ENSMUSP00000078024; ENSMUSG00000025702.
ENSMUST00000101032; ENSMUSP00000098594; ENSMUSG00000025702.
GeneIDi71779.
KEGGimmu:71779.
UCSCiuc009djz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005032 mRNA. Translation: BAB23759.1.
AK154976 mRNA. Translation: BAE32966.1.
BC050908 mRNA. Translation: AAH50908.1.
BC053090 mRNA. Translation: AAH53090.1.
CCDSiCCDS20451.1.
RefSeqiNP_001289312.1. NM_001302383.1.
NP_001289313.1. NM_001302384.1.
NP_001289314.1. NM_001302385.1.
NP_082196.1. NM_027920.5.
UniGeneiMm.27064.

3D structure databases

ProteinModelPortaliQ9DBD2.
SMRiQ9DBD2. Positions 68-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078024.

PTM databases

iPTMnetiQ9DBD2.
PhosphoSiteiQ9DBD2.

Proteomic databases

MaxQBiQ9DBD2.
PaxDbiQ9DBD2.
PRIDEiQ9DBD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079012; ENSMUSP00000078024; ENSMUSG00000025702.
ENSMUST00000101032; ENSMUSP00000098594; ENSMUSG00000025702.
GeneIDi71779.
KEGGimmu:71779.
UCSCiuc009djz.2. mouse.

Organism-specific databases

CTDi220972.
MGIiMGI:1919029. March8.

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110355.
HOGENOMiHOG000113483.
HOVERGENiHBG081957.
InParanoidiQ9DBD2.
KOiK10656.
OrthoDBiEOG091G0WJX.
PhylomeDBiQ9DBD2.
TreeFamiTF319557.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiMarch8. mouse.
PROiQ9DBD2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025702.
CleanExiMM_MARCH8.
ExpressionAtlasiQ9DBD2. baseline and differential.
GenevisibleiQ9DBD2. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMARH8_MOUSE
AccessioniPrimary (citable) accession number: Q9DBD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.