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Protein

cAMP-dependent protein kinase type I-alpha regulatory subunit

Gene

Prkar1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.By similarity

Miscellaneous

Two types of regulatory chains are found: type I, which predominates in skeletal muscle, and type II, which predominates in cardiac muscle.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei202cAMP 11
Binding sitei211cAMP 11
Binding sitei326cAMP 21
Binding sitei335cAMP 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 254cAMP 1Add BLAST118
Nucleotide bindingi255 – 381cAMP 2Add BLAST127

GO - Molecular functioni

GO - Biological processi

  • animal organ morphogenesis Source: MGI
  • cardiac muscle cell proliferation Source: MGI
  • cell proliferation Source: MGI
  • female meiotic nuclear division Source: Ensembl
  • heart development Source: MGI
  • mesoderm formation Source: MGI
  • negative regulation of activated T cell proliferation Source: MGI
  • negative regulation of cAMP-dependent protein kinase activity Source: MGI
  • negative regulation of meiotic nuclear division Source: Ensembl
  • negative regulation of protein kinase activity Source: MGI
  • protein phosphorylation Source: MGI
  • sarcomere organization Source: MGI

Keywordsi

LigandcAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-163615 PKA activation
R-MMU-164378 PKA activation in glucagon signalling
R-MMU-180024 DARPP-32 events
R-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-5610787 Hedgehog 'off' state
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type I-alpha regulatory subunit
Cleaved into the following chain:
Gene namesi
Name:Prkar1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:104878 Prkar1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002053781 – 381cAMP-dependent protein kinase type I-alpha regulatory subunitAdd BLAST381
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004217862 – 381cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processedAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylalanine; in cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processedBy similarity1
Modified residuei3PhosphoserineBy similarity1
Disulfide bondi18Interchain (with C-39)By similarity
Disulfide bondi39Interchain (with C-18)By similarity
Modified residuei75PhosphothreonineBy similarity1
Modified residuei77PhosphoserineBy similarity1
Modified residuei83PhosphoserineCombined sources1
Modified residuei101PhosphoserineCombined sources1
Modified residuei258PhosphoserineBy similarity1

Post-translational modificationi

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9DBC7
MaxQBiQ9DBC7
PaxDbiQ9DBC7
PRIDEiQ9DBC7

2D gel databases

REPRODUCTION-2DPAGEiIPI00762049
Q9DBC7

PTM databases

iPTMnetiQ9DBC7
PhosphoSitePlusiQ9DBC7
SwissPalmiQ9DBC7

Expressioni

Gene expression databases

BgeeiENSMUSG00000020612
ExpressionAtlasiQ9DBC7 baseline and differential
GenevisibleiQ9DBC7 MM

Interactioni

Subunit structurei

The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. PRKAR1A also interacts with RFC2; the complex may be involved in cell survival. Interacts with AKAP4. Interacts with RARA; the interaction occurs in the presence of cAMP or FSH and regulates RARA transcriptional activity. Interacts with the phosphorylated form of PJA2. Interacts with PRKX; regulates this cAMP-dependent protein kinase (By similarity). Interacts with CBFA2T3. Interacts with smAKAP; this interaction may target PRKAR1A to the plasma membrane. Interacts with AICDA (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Bcl2l11O54918-12EBI-645677,EBI-526076

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202365, 4 interactors
DIPiDIP-32450N
IntActiQ9DBC7, 15 interactors
MINTiQ9DBC7
STRINGi10090.ENSMUSP00000056500

Structurei

3D structure databases

ProteinModelPortaliQ9DBC7
SMRiQ9DBC7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 136Dimerization and phosphorylationAdd BLAST135

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 100Pseudophosphorylation motif5

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOVERGENiHBG002025
InParanoidiQ9DBC7
KOiK04739
OMAiVQLCTVR
OrthoDBiEOG091G0F1K
TreeFamiTF314920

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DBC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGSMATSE EERSLRECEL YVQKHNIQAL LKDSIVQLCT TRPERPMAFL
60 70 80 90 100
REYFERLEKE EARQIQCLQK TGIRTDSRED EISPPPPNPV VKGRRRRGAI
110 120 130 140 150
SAEVYTEEDA ASYVRKVIPK DYKTMAALAK AIEKNVLFSH LDDNERSDIF
160 170 180 190 200
DAMFPVSFIA GETVIQQGDE GDNFYVIDQG EMDVYVNNEW ATSVGEGGSF
210 220 230 240 250
GELALIYGTP RAATVKAKTN VKLWGIDRDS YRRILMGSTL RKRKMYEEFL
260 270 280 290 300
SKVSILESLD KWERLTVADA LEPVQFEDGQ KIVVQGEPGD EFFIILEGTA
310 320 330 340 350
AVLQRRSENE EFVEVGRLGP SDYFGEIALL MNRPRAATVV ARGPLKCVKL
360 370 380
DRPRFERVLG PCSDILKRNI QQYNSFVSLS V
Length:381
Mass (Da):43,185
Last modified:January 23, 2007 - v3
Checksum:i08F164BB4528C63B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005039 mRNA Translation: BAB23766.1
AK027916 mRNA Translation: BAC25664.1
AK051068 mRNA Translation: BAC34516.1
AK145797 mRNA Translation: BAE26655.1
AK145860 mRNA Translation: BAE26704.1
AK147188 mRNA Translation: BAE27748.1
AK150427 mRNA Translation: BAE29550.1
AK151124 mRNA Translation: BAE30132.1
AK153227 mRNA Translation: BAE31820.1
BC003461 mRNA Translation: AAH03461.1
BC005697 mRNA Translation: AAH05697.1
AJ278427 Genomic DNA Translation: CAB94778.1
AJ278429 Genomic DNA Translation: CAB94718.1
CCDSiCCDS25583.1
RefSeqiNP_001300902.1, NM_001313973.1
NP_001300903.1, NM_001313974.1
NP_001300904.1, NM_001313975.1
NP_001300905.1, NM_001313976.1
NP_068680.1, NM_021880.3
XP_017169829.1, XM_017314340.1
UniGeneiMm.30039

Genome annotation databases

EnsembliENSMUST00000049527; ENSMUSP00000056500; ENSMUSG00000020612
ENSMUST00000106677; ENSMUSP00000102288; ENSMUSG00000020612
GeneIDi19084
KEGGimmu:19084
UCSCiuc007mcu.1 mouse

Similar proteinsi

Entry informationi

Entry nameiKAP0_MOUSE
AccessioniPrimary (citable) accession number: Q9DBC7
Secondary accession number(s): Q3UKU7, Q9JHR5, Q9JHR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health