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Protein

General transcription factor IIH subunit 1

Gene

Gtf2h1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.By similarity

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 1
Alternative name(s):
Basic transcription factor 2 62 kDa subunit
Short name:
BTF2 p62
General transcription factor IIH polypeptide 1
TFIIH basal transcription factor complex p62 subunit
Gene namesi
Name:Gtf2h1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1277216. Gtf2h1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192461 – 547General transcription factor IIH subunit 1Add BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei239N6-acetyllysineCombined sources1
Modified residuei338PhosphoserineBy similarity1
Modified residuei356PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9DBA9.
MaxQBiQ9DBA9.
PaxDbiQ9DBA9.
PeptideAtlasiQ9DBA9.
PRIDEiQ9DBA9.

PTM databases

iPTMnetiQ9DBA9.
PhosphoSitePlusiQ9DBA9.

Expressioni

Gene expression databases

CleanExiMM_GTF2H1.

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription. Interacts with PUF60.By similarity

Protein-protein interaction databases

BioGridi200111. 4 interactors.
IntActiQ9DBA9. 1 interactor.
STRINGi10090.ENSMUSP00000006774.

Structurei

3D structure databases

ProteinModelPortaliQ9DBA9.
SMRiQ9DBA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 154BSD 1PROSITE-ProRule annotationAdd BLAST56
Domaini179 – 231BSD 2PROSITE-ProRule annotationAdd BLAST53

Sequence similaritiesi

Belongs to the TFB1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2074. Eukaryota.
ENOG410XRI6. LUCA.
HOGENOMiHOG000006589.
HOVERGENiHBG060375.
InParanoidiQ9DBA9.
KOiK03141.
PhylomeDBiQ9DBA9.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR005607. BSD_dom.
IPR011993. PH-like_dom_sf.
IPR027079. Tfb1/GTF2H1.
IPR013876. TFIIH_BTF_p62_N.
PANTHERiPTHR12856. PTHR12856. 1 hit.
PfamiView protein in Pfam
PF03909. BSD. 1 hit.
PF08567. PH_TFIIH. 1 hit.
SMARTiView protein in SMART
SM00751. BSD. 2 hits.
PROSITEiView protein in PROSITE
PS50858. BSD. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9DBA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSSEEVLL IVKKVRQKKQ DGALYLMAER IAWAPEGKDR FTISHMYADI
60 70 80 90 100
KCQKISPEGK AKIQLQLVLH AGDTTNFHFS NESTAVKERD AVKDLLQQLL
110 120 130 140 150
PKFKRKANKE LEEKNRMLQE DPVLFQLYKD LVVSQVISAE EFWANRLNVN
160 170 180 190 200
ATDSSTSSHK QDVGISAAFL ADVRPQTDGC NGLRYNLTSD IIESIFRTYP
210 220 230 240 250
AVKMKYAETV PHNMTEKEFW TRFFQSHYFH RDRLNTGSKD LFAECAKIDE
260 270 280 290 300
KGLKTMVSLG VKNPMLDLTS LEDKPLDEGY SISSVPSTSN SKSIKENSNA
310 320 330 340 350
AIIKRFNHHS AMVLAAGLRK QQAQNGQNGE PSSVDGNSGD TDCFQPAVKR
360 370 380 390 400
AKLQESIEYE DLGNNNSVKT IALNLKKSDR YYHGPTPIQS LQYATSQDII
410 420 430 440 450
NSFQSIRQEM EAYTPKLTQV LSSSAASSTI TALSPGGALM QGGTQQAVNQ
460 470 480 490 500
MVPNDIQSEL KHLYVAVGEL LRHFWSCFPV NTPFLEEKVV KMKSNLERFQ
510 520 530 540
VTKLCPFQEK IRRQYLSTNL VSHIEEMLQT AYNKLHTWQS RRLMKKT
Length:547
Mass (Da):61,851
Last modified:October 18, 2001 - v2
Checksum:i0167316D11F87969
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137I → F in BAB23789 (PubMed:16141072).Curated1
Sequence conflicti208E → G in BAB23789 (PubMed:16141072).Curated1
Sequence conflicti281S → G in BAB23789 (PubMed:16141072).Curated1
Sequence conflicti537T → A in BAB23789 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002366 mRNA. Translation: CAA05340.1.
AK005065 mRNA. Translation: BAB23789.1.
CCDSiCCDS21287.1.
RefSeqiNP_001278004.1. NM_001291075.1.
NP_032212.3. NM_008186.4.
UniGeneiMm.22700.

Genome annotation databases

GeneIDi14884.
KEGGimmu:14884.

Similar proteinsi

Entry informationi

Entry nameiTF2H1_MOUSE
AccessioniPrimary (citable) accession number: Q9DBA9
Secondary accession number(s): O35637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 18, 2001
Last modified: March 28, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome