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Protein

Peroxisomal leader peptide-processing protease

Gene

Tysnd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Peroxisomal protease that mediates both the removal of the leader peptide from proteins containing a PTS2 target sequence and processes several PTS1-containing proteins. Catalyzes the processing of PTS1-proteins involved in the peroxisomal beta-oxidation of fatty acids (By similarity).By similarity1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide (NEM). Not affected by leupeptin or trans-epoxysuccinyl-l-leucylamido-(4-gianidino) butane (E64).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei374Charge relay systemBy similarity1
Active sitei410Charge relay systemBy similarity1
Active sitei483Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.286.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal leader peptide-processing protease (EC:3.4.21.-)
Alternative name(s):
Trypsin domain-containing protein 1
Cleaved into the following 2 chains:
Gene namesi
Name:Tysnd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1919017. Tysnd1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002861271 – 568Peroxisomal leader peptide-processing proteaseAdd BLAST568
ChainiPRO_00002861281 – 110Peroxisomal leader peptide-processing protease, 10 kDa formAdd BLAST110
ChainiPRO_0000286129111 – 568Peroxisomal leader peptide-processing protease, 49 kDa formAdd BLAST458

Post-translational modificationi

Self-cleavage gives rise to an N-terminal 10-kDa fragment and C-terminal 49-kDa fragment upon import into the peroxisomes. The full-lengh TYSND1 is the active the proteolytic processing of PTS1- and PTS2-proteins and in self-cleavage, and intermolecular self-cleavage of TYSND1 down-regulates its protease activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei110 – 111Cleavage2

Proteomic databases

EPDiQ9DBA6.
MaxQBiQ9DBA6.
PaxDbiQ9DBA6.
PeptideAtlasiQ9DBA6.
PRIDEiQ9DBA6.

PTM databases

iPTMnetiQ9DBA6.
PhosphoSitePlusiQ9DBA6.
SwissPalmiQ9DBA6.

Expressioni

Inductioni

By the proliferator-activated receptor alpha agonist bezafibrate.1 Publication

Gene expression databases

BgeeiENSMUSG00000020087.
CleanExiMM_TYSND1.
GenevisibleiQ9DBA6. MM.

Interactioni

Subunit structurei

Homodimer. Forms a heterodimer with the C-terminal cleavage product (49 kDa form). Forms a heterodimer with the N-terminal cleavage product (10 kDa form). Interacts with PEX5. Interacts with LONP2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9DBA6. 1 interactor.
MINTiMINT-4128149.
STRINGi10090.ENSMUSP00000020284.

Structurei

3D structure databases

ProteinModelPortaliQ9DBA6.
SMRiQ9DBA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni332 – 568Serine proteaseAdd BLAST237

Sequence similaritiesi

Belongs to the peptidase S1B family.Curated
Contains 1 peptidase S1 domain.Curated

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
GeneTreeiENSGT00390000014627.
HOGENOMiHOG000050236.
HOVERGENiHBG101259.
InParanoidiQ9DBA6.
OMAiCWKAREW.
OrthoDBiEOG091G06CU.
PhylomeDBiQ9DBA6.
TreeFamiTF331254.

Family and domain databases

InterProiIPR017345. Pept_S1A_Tysnd1.
IPR009003. Peptidase_S1_PA.
[Graphical view]
PIRSFiPIRSF037989. Peptidase_S1B_Tysnd1. 1 hit.
SUPFAMiSSF50494. SSF50494. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DBA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRQWGPSMR VAEQAGCVVS ASRAGQPDAG SWSCSGVILS RNPGLVLCHG
60 70 80 90 100
GIFTPFLRTG SAALTQTGTA FLPGDSCSDD LRLHVQWGPT AASPAGRADQ
110 120 130 140 150
ELPGLCTPQC ASLGLEPGAP SRARARPLQP PRPAQLLLLL SCPAFRSHFA
160 170 180 190 200
RLFGADAVDQ WHFVSSAPDD AVSEEEEEDQ LRALGWFALL RVQRGAAAEE
210 220 230 240 250
RRGPVVTVAP LGAVVKGAPL LACGSPFGAF CPDIFLNTLS RGVLSNAAGP
260 270 280 290 300
LLLTDARCLP GTEGGGVFAA RPAGALVALV AAPLCWKARE WVGLTLLCAA
310 320 330 340 350
APLLQVARWA LARLHPGSAS LSVLLPPPDV STPRGLPLRD LGPPWAAAAV
360 370 380 390 400
LVECGTVWGS GVVVAPRLVV TCRHVAPREA ARVLVHSATP KNVAIWGQVV
410 420 430 440 450
FATQETSPYD IAVVSLEEEL NGVPTPVPAG HFHEGEPVSV VGFGVFGQAC
460 470 480 490 500
GPSVTSGILS AVVRVDGSPV MLQTTCAVHG GSSGGPLFSS GSGDLLGIVA
510 520 530 540 550
SNTRDNNTGA TYPHLNFSIP ITVLQPALKQ YSQTGDLGGL RELDHTTEPV
560
RVVWRLQRPL SEVPRSKL
Length:568
Mass (Da):59,066
Last modified:June 1, 2001 - v1
Checksum:iB7BED91B4DB50E5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005069 mRNA. Translation: BAB23793.1.
AK154513 mRNA. Translation: BAE32642.1.
BC119320 mRNA. Translation: AAI19321.2.
CCDSiCCDS48575.1.
RefSeqiNP_001259019.1. NM_001272090.1.
NP_001259020.1. NM_001272091.1.
NP_001259021.1. NM_001272092.1.
NP_082188.1. NM_027912.1.
UniGeneiMm.71514.

Genome annotation databases

EnsembliENSMUST00000020284; ENSMUSP00000020284; ENSMUSG00000020087.
GeneIDi71767.
KEGGimmu:71767.
UCSCiuc007fgh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005069 mRNA. Translation: BAB23793.1.
AK154513 mRNA. Translation: BAE32642.1.
BC119320 mRNA. Translation: AAI19321.2.
CCDSiCCDS48575.1.
RefSeqiNP_001259019.1. NM_001272090.1.
NP_001259020.1. NM_001272091.1.
NP_001259021.1. NM_001272092.1.
NP_082188.1. NM_027912.1.
UniGeneiMm.71514.

3D structure databases

ProteinModelPortaliQ9DBA6.
SMRiQ9DBA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DBA6. 1 interactor.
MINTiMINT-4128149.
STRINGi10090.ENSMUSP00000020284.

Protein family/group databases

MEROPSiS01.286.

PTM databases

iPTMnetiQ9DBA6.
PhosphoSitePlusiQ9DBA6.
SwissPalmiQ9DBA6.

Proteomic databases

EPDiQ9DBA6.
MaxQBiQ9DBA6.
PaxDbiQ9DBA6.
PeptideAtlasiQ9DBA6.
PRIDEiQ9DBA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020284; ENSMUSP00000020284; ENSMUSG00000020087.
GeneIDi71767.
KEGGimmu:71767.
UCSCiuc007fgh.2. mouse.

Organism-specific databases

CTDi219743.
MGIiMGI:1919017. Tysnd1.

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
GeneTreeiENSGT00390000014627.
HOGENOMiHOG000050236.
HOVERGENiHBG101259.
InParanoidiQ9DBA6.
OMAiCWKAREW.
OrthoDBiEOG091G06CU.
PhylomeDBiQ9DBA6.
TreeFamiTF331254.

Miscellaneous databases

PROiQ9DBA6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020087.
CleanExiMM_TYSND1.
GenevisibleiQ9DBA6. MM.

Family and domain databases

InterProiIPR017345. Pept_S1A_Tysnd1.
IPR009003. Peptidase_S1_PA.
[Graphical view]
PIRSFiPIRSF037989. Peptidase_S1B_Tysnd1. 1 hit.
SUPFAMiSSF50494. SSF50494. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiTYSD1_MOUSE
AccessioniPrimary (citable) accession number: Q9DBA6
Secondary accession number(s): Q0VE90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.