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Protein

Ribosomal RNA-processing protein 8

Gene

Rrp8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei282S-adenosyl-L-methionineBy similarity1
Binding sitei317S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei335S-adenosyl-L-methionineBy similarity1
Binding sitei347S-adenosyl-L-methionineBy similarity1
Binding sitei348S-adenosyl-L-methionineBy similarity1
Binding sitei364S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Methyltransferase, Repressor, Transferase
Biological processrRNA processing, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-427359 SIRT1 negatively regulates rRNA expression

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA-processing protein 8 (EC:2.1.1.-)
Alternative name(s):
Cerebral protein 1 homolog
Gene namesi
Name:Rrp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1914251 Rrp8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000840911 – 457Ribosomal RNA-processing protein 8Add BLAST457

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62PhosphoserineCombined sources1
Modified residuei64PhosphoserineCombined sources1
Modified residuei105PhosphoserineBy similarity1
Modified residuei172PhosphoserineBy similarity1
Modified residuei177PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9DB85
MaxQBiQ9DB85
PaxDbiQ9DB85
PeptideAtlasiQ9DB85
PRIDEiQ9DB85

PTM databases

iPTMnetiQ9DB85
PhosphoSitePlusiQ9DB85

Expressioni

Gene expression databases

BgeeiENSMUSG00000030888
CleanExiMM_1500003O22RIK
ExpressionAtlasiQ9DB85 baseline and differential
GenevisibleiQ9DB85 MM

Interactioni

Subunit structurei

Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi221741, 3 interactors
IntActiQ9DB85, 1 interactor
MINTiQ9DB85
STRINGi10090.ENSMUSP00000095752

Structurei

3D structure databases

ProteinModelPortaliQ9DB85
SMRiQ9DB85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi65 – 69Poly-Glu5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3045 Eukaryota
ENOG4111J8Z LUCA
GeneTreeiENSGT00390000006189
HOGENOMiHOG000164004
InParanoidiQ9DB85
KOiK14850
PhylomeDBiQ9DB85
TreeFamiTF313749

Family and domain databases

InterProiView protein in InterPro
IPR007823 RRP8
IPR029063 SAM-dependent_MTases
PANTHERiPTHR12787 PTHR12787, 1 hit
PfamiView protein in Pfam
PF05148 Methyltransf_8, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DB85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEEPEWVEA APAIVGLGAA TAQVRPATAP PVKGRKRRHL LATLRALEAA
60 70 80 90 100
SLSQQTPSLP GSDSEEEEEV GRKKRHLQRP SLASVSKEVG KKRKGKCQKQ
110 120 130 140 150
APSISDSEGK EIRRKCHRQA PPLGGVSAGE EKGKRKCQEY SSLHLTQPLD
160 170 180 190 200
SVDQTVHNSR TSTATIDPSK PSPESMSPNS SHTLSRKQWR NRQKNKRRHK
210 220 230 240 250
NKFRPLQTPE QAPPKASIEE TEVPPVPKSD SQESRAGALR ARMTQRLDGA
260 270 280 290 300
RFRYLNEQLY SGPSSAARRL FQEDPEAFLL YHRGFQRQVK KWPLHPVDRI
310 320 330 340 350
AKDLRQKPAS LVVADFGCGD CRLASSVRNP VHCFDLASLD PRVTVCDMAQ
360 370 380 390 400
VPLEDESVDV AVFCLSLMGT NIRDFLEEAN RVLKTGGLLK VAEVSSRFED
410 420 430 440 450
IRTFLGAVTK LGFKIIYKDL TNSHFFLFDF EKTGPPRVGP KAQLSGLKLQ

PCLYKRR
Length:457
Mass (Da):51,066
Last modified:June 1, 2001 - v1
Checksum:iA9DB8CB932E1DB3C
GO

Sequence cautioni

The sequence BAC37008 differs from that shown. Reason: Frameshift at position 22.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30P → R in BAE41665 (PubMed:16141072).Curated1
Sequence conflicti30P → R in BAE42891 (PubMed:16141072).Curated1
Sequence conflicti30P → R in BAE42874 (PubMed:16141072).Curated1
Sequence conflicti151S → N in BAE41665 (PubMed:16141072).Curated1
Sequence conflicti151S → N in BAE42891 (PubMed:16141072).Curated1
Sequence conflicti151S → N in BAE42874 (PubMed:16141072).Curated1
Sequence conflicti173P → H in BAE32883 (PubMed:16141072).Curated1
Sequence conflicti268R → Q in BAE41665 (PubMed:16141072).Curated1
Sequence conflicti268R → Q in BAE42891 (PubMed:16141072).Curated1
Sequence conflicti268R → Q in BAE42874 (PubMed:16141072).Curated1
Sequence conflicti370T → N in BAE32883 (PubMed:16141072).Curated1
Sequence conflicti385T → P in BAE41665 (PubMed:16141072).Curated1
Sequence conflicti385T → P in BAE42891 (PubMed:16141072).Curated1
Sequence conflicti385T → P in BAE42874 (PubMed:16141072).Curated1
Sequence conflicti394V → A in BAE41665 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005138 mRNA Translation: BAB23836.1
AK045361 mRNA Translation: BAC32326.1
AK077786 mRNA Translation: BAC37008.1 Frameshift.
AK154858 mRNA Translation: BAE32883.1
AK170257 mRNA Translation: BAE41665.1
AK172189 mRNA Translation: BAE42874.1
AK172224 mRNA Translation: BAE42891.1
BC022923 mRNA Translation: AAH22923.1
BC046799 mRNA Translation: AAH46799.1
CCDSiCCDS21658.1
RefSeqiNP_080173.1, NM_025897.2
UniGeneiMm.231400

Genome annotation databases

EnsembliENSMUST00000033179; ENSMUSP00000033179; ENSMUSG00000030888
GeneIDi101867
KEGGimmu:101867
UCSCiuc009iyz.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRRP8_MOUSE
AccessioniPrimary (citable) accession number: Q9DB85
Secondary accession number(s): Q3T9X9
, Q3TDD7, Q3U3A5, Q8BHW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health