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Protein

NADH-cytochrome b5 reductase 1

Gene

Cyb5r1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.By similarity

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi136 – 166FADBy similarityAdd BLAST31
Nucleotide bindingi175 – 210FADBy similarityAdd BLAST36

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 1 (EC:1.6.2.2)
Short name:
b5R.1
Alternative name(s):
NAD(P)H:quinone oxidoreductase type 3 polypeptide A2
Gene namesi
Name:Cyb5r1
Synonyms:Nqo3a2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1919267 Cyb5r1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002875461 – 305NADH-cytochrome b5 reductase 1Add BLAST305

Proteomic databases

EPDiQ9DB73
PaxDbiQ9DB73
PeptideAtlasiQ9DB73
PRIDEiQ9DB73

PTM databases

iPTMnetiQ9DB73
PhosphoSitePlusiQ9DB73

Expressioni

Gene expression databases

BgeeiENSMUSG00000026456
CleanExiMM_CYB5R1
ExpressionAtlasiQ9DB73 baseline and differential
GenevisibleiQ9DB73 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027726

Structurei

3D structure databases

ProteinModelPortaliQ9DB73
SMRiQ9DB73
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 156FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0534 Eukaryota
COG0543 LUCA
GeneTreeiENSGT00390000008881
HOGENOMiHOG000175005
HOVERGENiHBG052580
InParanoidiQ9DB73
KOiK00326
OMAiPTAHHVL
OrthoDBiEOG091G0EIS
PhylomeDBiQ9DB73
TreeFamiTF314333

Family and domain databases

InterProiView protein in InterPro
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR001834 NADH-Cyt_B5_reductase
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00371 FPNCR
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DB73-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGIQPSPVLL ASLGVGLLTL LGLALGTYLV RRSRRPQVTL QDPDEKYLLR
60 70 80 90 100
LLDKTTVSHN TRRFRFALPT AHHILGLPVG KHVYLSARID GSLVIRPYTP
110 120 130 140 150
VTSDEDQGYV DLVIKVYLKG VHPKFPEGGK MSQYLDSLKI GDMVEFRGPS
160 170 180 190 200
GLLSYAGKGN FNIQPNKKSP PELRVAKKLG MIAGGTGITP MLQLIRAILK
210 220 230 240 250
VPEDPTQCFL LFANQTERDI ILREDLEELQ AQYPNRFKLW FTLDSPPEDW
260 270 280 290 300
TYSKGFVTAD MIQEHLPAPA EDVLLLLCGP PPMVQLACHP NLDKLGYSQK

MRFTY
Length:305
Mass (Da):34,135
Last modified:June 1, 2001 - v1
Checksum:i847B9AAFD99ED035
GO
Isoform 2 (identifier: Q9DB73-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     159-228: GNFNIQPNKK...DIILREDLEE → ESACVHGCTW...DDPSTVPCLW
     229-305: Missing.

Note: No experimental confirmation available.
Show »
Length:228
Mass (Da):25,305
Checksum:i85FCC210489EDD32
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025557159 – 228GNFNI…EDLEE → ESACVHGCTWAKSPEATMLY GKADFTLWRLPEETYASPSF TKDFPLAKDQKRKLFSSSHG DDPSTVPCLW in isoform 2. 1 PublicationAdd BLAST70
Alternative sequenceiVSP_025558229 – 305Missing in isoform 2. 1 PublicationAdd BLAST77

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005159 mRNA Translation: BAB23850.1
AK159663 mRNA Translation: BAE35270.1
AK167178 mRNA Translation: BAE39313.1
BC016266 mRNA Translation: AAH16266.1
BC024618 mRNA Translation: AAH24618.1
CCDSiCCDS15308.1 [Q9DB73-1]
RefSeqiNP_082333.1, NM_028057.2 [Q9DB73-1]
UniGeneiMm.280230
Mm.490981

Genome annotation databases

EnsembliENSMUST00000027726; ENSMUSP00000027726; ENSMUSG00000026456 [Q9DB73-1]
ENSMUST00000154237; ENSMUSP00000133385; ENSMUSG00000026456 [Q9DB73-2]
GeneIDi72017
KEGGimmu:72017
UCSCiuc007cru.1 mouse [Q9DB73-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNB5R1_MOUSE
AccessioniPrimary (citable) accession number: Q9DB73
Secondary accession number(s): Q91W81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health