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Protein

Prostamide/prostaglandin F synthase

Gene

Fam213b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of prostaglandin-ethanolamide H2 (prostamide H2) to prostamide F(2alpha) with NADPH as proton donor. Also able to reduce prostaglandin H2 to prostaglandin F(2alpha).1 Publication

Catalytic activityi

Thioredoxin + prostaglandin H2 = thioredoxin disulfide + prostaglandin F(2-alpha).1 Publication

GO - Molecular functioni

  • oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: UniProtKB
  • prostaglandin-F synthase activity Source: UniProtKB
  • thioredoxin peroxidase activity Source: Reactome

GO - Biological processi

  • cyclooxygenase pathway Source: Reactome
  • prostaglandin biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Prostaglandin biosynthesis, Prostaglandin metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.11.1.20. 3474.
ReactomeiR-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

Chemistry databases

SwissLipidsiSLP:000001101.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostamide/prostaglandin F synthase (EC:1.11.1.20)
Short name:
Prostamide/PG F synthase
Short name:
Prostamide/PGF synthase
Gene namesi
Name:Fam213b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1913719. Fam213b.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: MGI
  • endoplasmic reticulum Source: UniProtKB
  • extracellular exosome Source: MGI
  • myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44C → S: Almost abolishes enzyme activity. 1 Publication1
Mutagenesisi47C → S: Decreased enzyme activity to retain 63% of wild type activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002846381 – 201Prostamide/prostaglandin F synthaseAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei108PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9DB60.
PaxDbiQ9DB60.
PeptideAtlasiQ9DB60.
PRIDEiQ9DB60.

PTM databases

iPTMnetiQ9DB60.
PhosphoSitePlusiQ9DB60.

Expressioni

Tissue specificityi

Mainly present in brain and spinal cord. In spinal cord, present in the superficial layer of the dorsal horn, in motor neurons of the ventral horn and in glia of the white matter of the spinal cord. In brain, expressed preferentially in the white matter bundles of the entire CNS of adult with less marked expression in neuronal cell bodies. Colocalizes with MBP in myelin sheaths but not in axons. Localizes to myelin sheaths (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000029059.
CleanExiMM_2810405K02RIK.
ExpressionAtlasiQ9DB60. baseline and differential.
GenevisibleiQ9DB60. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030935.

Structurei

3D structure databases

ProteinModelPortaliQ9DB60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4498. Eukaryota.
ENOG410YFY9. LUCA.
GeneTreeiENSGT00530000063724.
HOVERGENiHBG106494.
InParanoidiQ9DB60.
KOiK15717.
OMAiGQATWCL.
OrthoDBiEOG091G0WTG.
PhylomeDBiQ9DB60.
TreeFamiTF313804.

Family and domain databases

InterProiIPR032802. FAM213.
IPR032801. FAM213/AAED1.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR28630. PTHR28630. 1 hit.
PTHR28630:SF2. PTHR28630:SF2. 1 hit.
PfamiPF13911. AhpC-TSA_2. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DB60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVVDLGRVG ACVLKHAVTG EAVELRSLWQ EKACVVAGLR RFGCMVCRWI
60 70 80 90 100
AQDLSNLRSI LDQHDVRLVG VGPEALGLQE FLDGGYFSGE LYLDESKQIY
110 120 130 140 150
KELGFKRYNS LSILPAALGK PVRDVASKAK AVGIQGNLSG DLLQSGGLLV
160 170 180 190 200
VSKGGDKVLL HFIQKSPGDY VPQENILQAL GISAEVCSSK PPQCDEEVCG

R
Length:201
Mass (Da):21,670
Last modified:June 1, 2001 - v1
Checksum:i4F392EF111AC6B42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005188 mRNA. Translation: BAB23870.1.
AL607032 Genomic DNA. Translation: CAM15790.1.
BC030453 mRNA. Translation: AAH30453.1.
CCDSiCCDS19016.1.
RefSeqiNP_079858.2. NM_025582.3.
UniGeneiMm.41868.

Genome annotation databases

EnsembliENSMUST00000030935; ENSMUSP00000030935; ENSMUSG00000029059.
GeneIDi66469.
KEGGimmu:66469.
UCSCiuc008wch.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005188 mRNA. Translation: BAB23870.1.
AL607032 Genomic DNA. Translation: CAM15790.1.
BC030453 mRNA. Translation: AAH30453.1.
CCDSiCCDS19016.1.
RefSeqiNP_079858.2. NM_025582.3.
UniGeneiMm.41868.

3D structure databases

ProteinModelPortaliQ9DB60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030935.

Chemistry databases

SwissLipidsiSLP:000001101.

PTM databases

iPTMnetiQ9DB60.
PhosphoSitePlusiQ9DB60.

Proteomic databases

MaxQBiQ9DB60.
PaxDbiQ9DB60.
PeptideAtlasiQ9DB60.
PRIDEiQ9DB60.

Protocols and materials databases

DNASUi66469.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030935; ENSMUSP00000030935; ENSMUSG00000029059.
GeneIDi66469.
KEGGimmu:66469.
UCSCiuc008wch.1. mouse.

Organism-specific databases

CTDi127281.
MGIiMGI:1913719. Fam213b.

Phylogenomic databases

eggNOGiKOG4498. Eukaryota.
ENOG410YFY9. LUCA.
GeneTreeiENSGT00530000063724.
HOVERGENiHBG106494.
InParanoidiQ9DB60.
KOiK15717.
OMAiGQATWCL.
OrthoDBiEOG091G0WTG.
PhylomeDBiQ9DB60.
TreeFamiTF313804.

Enzyme and pathway databases

BRENDAi1.11.1.20. 3474.
ReactomeiR-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

Miscellaneous databases

PROiQ9DB60.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029059.
CleanExiMM_2810405K02RIK.
ExpressionAtlasiQ9DB60. baseline and differential.
GenevisibleiQ9DB60. MM.

Family and domain databases

InterProiIPR032802. FAM213.
IPR032801. FAM213/AAED1.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR28630. PTHR28630. 1 hit.
PTHR28630:SF2. PTHR28630:SF2. 1 hit.
PfamiPF13911. AhpC-TSA_2. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGFS_MOUSE
AccessioniPrimary (citable) accession number: Q9DB60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.