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Protein

Mediator of RNA polymerase II transcription subunit 27

Gene

Med27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 27
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 8
Short name:
CRSP complex subunit 8
Mediator complex subunit 27
Gene namesi
Name:Med27
Synonyms:Crsp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916225. Med27.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Mediator of RNA polymerase II transcription subunit 27PRO_0000079362Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei132 – 1321PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9DB40.
MaxQBiQ9DB40.
PaxDbiQ9DB40.
PeptideAtlasiQ9DB40.
PRIDEiQ9DB40.

PTM databases

iPTMnetiQ9DB40.
PhosphoSiteiQ9DB40.

Expressioni

Gene expression databases

BgeeiQ9DB40.
CleanExiMM_MED27.
ExpressionAtlasiQ9DB40. baseline and differential.
GenevisibleiQ9DB40. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Protein-protein interaction databases

BioGridi213151. 1 interaction.
IntActiQ9DB40. 4 interactions.
MINTiMINT-4128092.
STRINGi10090.ENSMUSP00000028139.

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 27 family.Curated

Phylogenomic databases

eggNOGiENOG410IFPB. Eukaryota.
ENOG410ZHQY. LUCA.
GeneTreeiENSGT00390000012207.
HOGENOMiHOG000043064.
HOVERGENiHBG104472.
InParanoidiQ9DB40.
KOiK15170.
OMAiQKRQRLP.
OrthoDBiEOG7J1806.
PhylomeDBiQ9DB40.
TreeFamiTF323728.

Family and domain databases

InterProiIPR021627. Mediator_Med27.
[Graphical view]
PfamiPF11571. Med27. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q9DB40-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADVLSVGVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK
60 70 80 90 100
AFIANFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK
110 120 130 140 150
TPLYSQLLQA YKWSNKLQYH AGLASGLLNQ QSLKRSANQM GVSAKRRPKA
160 170 180 190 200
QPTTLVLPPQ YVDDVISRID RMFPEMSIHL SRPNGTSAML LVTLGKVLKV
210 220 230 240 250
IVVMRSLFID RTIVKGYNES VYTEDGKLDI WSKSSYQVFQ KVTDHATTAL
260 270 280 290 300
LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR
310
TLEAFHDTCR Q
Length:311
Mass (Da):35,298
Last modified:November 22, 2005 - v2
Checksum:i60E208A64A2BA6BF
GO
Isoform 2Curated (identifier: Q9DB40-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     192-208: VTLGKVLKVIVVMRSLF → ILINSSAQSITGPWFWS
     209-311: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:208
Mass (Da):23,156
Checksum:i7DC2A91F85AB09C3
GO

Sequence cautioni

The sequence AAH16537.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei192 – 20817VTLGK…MRSLF → ILINSSAQSITGPWFWS in isoform 2. 1 PublicationVSP_051870Add
BLAST
Alternative sequencei209 – 311103Missing in isoform 2. 1 PublicationVSP_051871Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005251 mRNA. Translation: BAB23906.2.
AK009764 mRNA. Translation: BAB26488.2.
AK147181 mRNA. Translation: BAE27743.1.
AL772213, AL929437 Genomic DNA. Translation: CAM13399.1.
AL929437, AL772213 Genomic DNA. Translation: CAM26819.1.
BC016537 mRNA. Translation: AAH16537.1. Different initiation.
CCDSiCCDS15853.1. [Q9DB40-1]
CCDS71014.1. [Q9DB40-2]
RefSeqiNP_081172.2. NM_026896.5. [Q9DB40-1]
NP_083891.1. NM_029615.1. [Q9DB40-2]
UniGeneiMm.330109.

Genome annotation databases

EnsembliENSMUST00000028139; ENSMUSP00000028139; ENSMUSG00000026799. [Q9DB40-1]
ENSMUST00000113830; ENSMUSP00000109461; ENSMUSG00000026799. [Q9DB40-2]
GeneIDi68975.
KEGGimmu:68975.
UCSCiuc008izr.2. mouse. [Q9DB40-2]
uc008izs.2. mouse. [Q9DB40-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005251 mRNA. Translation: BAB23906.2.
AK009764 mRNA. Translation: BAB26488.2.
AK147181 mRNA. Translation: BAE27743.1.
AL772213, AL929437 Genomic DNA. Translation: CAM13399.1.
AL929437, AL772213 Genomic DNA. Translation: CAM26819.1.
BC016537 mRNA. Translation: AAH16537.1. Different initiation.
CCDSiCCDS15853.1. [Q9DB40-1]
CCDS71014.1. [Q9DB40-2]
RefSeqiNP_081172.2. NM_026896.5. [Q9DB40-1]
NP_083891.1. NM_029615.1. [Q9DB40-2]
UniGeneiMm.330109.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213151. 1 interaction.
IntActiQ9DB40. 4 interactions.
MINTiMINT-4128092.
STRINGi10090.ENSMUSP00000028139.

PTM databases

iPTMnetiQ9DB40.
PhosphoSiteiQ9DB40.

Proteomic databases

EPDiQ9DB40.
MaxQBiQ9DB40.
PaxDbiQ9DB40.
PeptideAtlasiQ9DB40.
PRIDEiQ9DB40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028139; ENSMUSP00000028139; ENSMUSG00000026799. [Q9DB40-1]
ENSMUST00000113830; ENSMUSP00000109461; ENSMUSG00000026799. [Q9DB40-2]
GeneIDi68975.
KEGGimmu:68975.
UCSCiuc008izr.2. mouse. [Q9DB40-2]
uc008izs.2. mouse. [Q9DB40-1]

Organism-specific databases

CTDi9442.
MGIiMGI:1916225. Med27.

Phylogenomic databases

eggNOGiENOG410IFPB. Eukaryota.
ENOG410ZHQY. LUCA.
GeneTreeiENSGT00390000012207.
HOGENOMiHOG000043064.
HOVERGENiHBG104472.
InParanoidiQ9DB40.
KOiK15170.
OMAiQKRQRLP.
OrthoDBiEOG7J1806.
PhylomeDBiQ9DB40.
TreeFamiTF323728.

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

PROiQ9DB40.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DB40.
CleanExiMM_MED27.
ExpressionAtlasiQ9DB40. baseline and differential.
GenevisibleiQ9DB40. MM.

Family and domain databases

InterProiIPR021627. Mediator_Med27.
[Graphical view]
PfamiPF11571. Med27. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum, HeartImported and TongueImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/NImported.
    Tissue: Mammary glandImported.

Entry informationi

Entry nameiMED27_MOUSE
AccessioniPrimary (citable) accession number: Q9DB40
Secondary accession number(s): A2AJV2, Q78JI3, Q9D707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 22, 2005
Last modified: July 6, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.