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Protein

Mediator of RNA polymerase II transcription subunit 27

Gene

Med27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription by RNA polymerase II Source: MGI
  • stem cell population maintenance Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 27
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 8
Short name:
CRSP complex subunit 8
Mediator complex subunit 27
Gene namesi
Name:Med27
Synonyms:Crsp8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916225 Med27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000793621 – 311Mediator of RNA polymerase II transcription subunit 27Add BLAST311

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei132PhosphoserineBy similarity1
Modified residuei134N6-methyllysineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ9DB40
PaxDbiQ9DB40
PeptideAtlasiQ9DB40
PRIDEiQ9DB40

PTM databases

iPTMnetiQ9DB40
PhosphoSitePlusiQ9DB40

Expressioni

Gene expression databases

BgeeiENSMUSG00000026799
CleanExiMM_MED27
ExpressionAtlasiQ9DB40 baseline and differential
GenevisibleiQ9DB40 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Protein-protein interaction databases

BioGridi213151, 1 interactor
IntActiQ9DB40, 4 interactors
MINTiQ9DB40
STRINGi10090.ENSMUSP00000028139

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 27 family.Curated

Phylogenomic databases

eggNOGiENOG410IFPB Eukaryota
ENOG410ZHQY LUCA
GeneTreeiENSGT00390000012207
HOGENOMiHOG000043064
HOVERGENiHBG104472
InParanoidiQ9DB40
KOiK15170
OMAiCSQKRQR
OrthoDBiEOG091G0BWJ
PhylomeDBiQ9DB40
TreeFamiTF323728

Family and domain databases

InterProiView protein in InterPro
IPR021627 Mediator_Med27
PfamiView protein in Pfam
PF11571 Med27, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q9DB40-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADVLSVGVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK
60 70 80 90 100
AFIANFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK
110 120 130 140 150
TPLYSQLLQA YKWSNKLQYH AGLASGLLNQ QSLKRSANQM GVSAKRRPKA
160 170 180 190 200
QPTTLVLPPQ YVDDVISRID RMFPEMSIHL SRPNGTSAML LVTLGKVLKV
210 220 230 240 250
IVVMRSLFID RTIVKGYNES VYTEDGKLDI WSKSSYQVFQ KVTDHATTAL
260 270 280 290 300
LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR
310
TLEAFHDTCR Q
Length:311
Mass (Da):35,298
Last modified:November 22, 2005 - v2
Checksum:i60E208A64A2BA6BF
GO
Isoform 2Curated (identifier: Q9DB40-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     192-208: VTLGKVLKVIVVMRSLF → ILINSSAQSITGPWFWS
     209-311: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:208
Mass (Da):23,156
Checksum:i7DC2A91F85AB09C3
GO

Sequence cautioni

The sequence AAH16537 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_051870192 – 208VTLGK…MRSLF → ILINSSAQSITGPWFWS in isoform 2. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_051871209 – 311Missing in isoform 2. 1 PublicationAdd BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005251 mRNA Translation: BAB23906.2
AK009764 mRNA Translation: BAB26488.2
AK147181 mRNA Translation: BAE27743.1
AL772213, AL929437 Genomic DNA Translation: CAM13399.1
AL929437, AL772213 Genomic DNA Translation: CAM26819.1
BC016537 mRNA Translation: AAH16537.1 Different initiation.
CCDSiCCDS15853.1 [Q9DB40-1]
CCDS71014.1 [Q9DB40-2]
RefSeqiNP_001277419.1, NM_001290490.1
NP_081172.2, NM_026896.5 [Q9DB40-1]
NP_083891.1, NM_029615.1 [Q9DB40-2]
UniGeneiMm.330109

Genome annotation databases

EnsembliENSMUST00000028139; ENSMUSP00000028139; ENSMUSG00000026799 [Q9DB40-1]
ENSMUST00000113830; ENSMUSP00000109461; ENSMUSG00000026799 [Q9DB40-2]
GeneIDi68975
KEGGimmu:68975
UCSCiuc008izr.2 mouse [Q9DB40-2]
uc008izs.2 mouse [Q9DB40-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMED27_MOUSE
AccessioniPrimary (citable) accession number: Q9DB40
Secondary accession number(s): A2AJV2, Q78JI3, Q9D707
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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