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Protein

Charged multivesicular body protein 2a

Gene

Chmp2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy
R-MMU-432720 Lysosome Vesicle Biogenesis
R-MMU-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 2a
Alternative name(s):
Chromatin-modifying protein 2a
Short name:
CHMP2a
Vacuolar protein sorting-associated protein 2
Short name:
mVps2
Gene namesi
Name:Chmp2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1916203 Chmp2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002114631 – 222Charged multivesicular body protein 2aAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei184PhosphoserineBy similarity1
Modified residuei185PhosphothreonineBy similarity1
Modified residuei188PhosphoserineBy similarity1
Modified residuei190PhosphoserineBy similarity1
Modified residuei203PhosphoserineBy similarity1

Post-translational modificationi

ISGylated in a CHMP5-dependent manner. Isgylation weakens and inhibits its interactions with VPS4A and VTA1 respectively (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9DB34
MaxQBiQ9DB34
PaxDbiQ9DB34
PeptideAtlasiQ9DB34
PRIDEiQ9DB34

PTM databases

iPTMnetiQ9DB34
PhosphoSitePlusiQ9DB34

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in brain, heart, liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000033916
CleanExiMM_CHMP2A
ExpressionAtlasiQ9DB34 baseline and differential
GenevisibleiQ9DB34 MM

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. In vitro, heteromerizes with CHMP3 (but not CHMP4) to form helical tubular structures that expose membrane-interacting sites on the outside whereas VPS4B can associate on the inside of the tubule. Interacts with CHMP1B, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C and CHMP5. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with MITD1. Interacts with VTA1; the interaction probably involves the open conformation of CHMP2A (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213137, 10 interactors
IntActiQ9DB34, 9 interactors
MINTiQ9DB34
STRINGi10090.ENSMUSP00000005711

Structurei

3D structure databases

ProteinModelPortaliQ9DB34
SMRiQ9DB34
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 222Interaction with VPS4BBy similarityAdd BLAST167
Regioni217 – 222Interaction with VTA1By similarity6

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili12 – 53Sequence analysisAdd BLAST42
Coiled coili195 – 222Sequence analysisAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi210 – 220MIT-interacting motifAdd BLAST11

Domaini

The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3230 Eukaryota
COG5491 LUCA
GeneTreeiENSGT00550000074737
HOGENOMiHOG000177218
HOVERGENiHBG107298
InParanoidiQ9DB34
KOiK12191
OMAiNMNRQLN
OrthoDBiEOG091G0RT1
PhylomeDBiQ9DB34
TreeFamiTF300118

Family and domain databases

InterProiView protein in InterPro
IPR005024 Snf7_fam
PfamiView protein in Pfam
PF03357 Snf7, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DB34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLLFGRRKT PEELLRQNQR ALNRAMRELD RERQKLETQE KKIIADIKKM
60 70 80 90 100
AKQGQMDAVR IMAKDLVRTR RYVRKFVLMR ANIQAVSLKI QTLKSNNSMA
110 120 130 140 150
QAMKGVTKAM GTMNRQLKLP QIQKIMMEFE RQAEIMDMKE EMMNDAIDDA
160 170 180 190 200
MGDEEDEEES DAVVSQVLDE LGLSLTDELS NLPSTGGSLS VAAGGKKAEA
210 220
TASALADADA DLEERLKNLR RD
Length:222
Mass (Da):25,134
Last modified:June 1, 2001 - v1
Checksum:iF2B86C623832E29E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005267 mRNA Translation: BAB23919.1
BC012230 mRNA Translation: AAH12230.1
CCDSiCCDS20824.1
RefSeqiNP_081161.1, NM_026885.3
XP_006540408.1, XM_006540345.3
UniGeneiMm.295670

Genome annotation databases

EnsembliENSMUST00000005711; ENSMUSP00000005711; ENSMUSG00000033916
ENSMUST00000210587; ENSMUSP00000148218; ENSMUSG00000033916
GeneIDi68953
KEGGimmu:68953
UCSCiuc009ffc.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCHM2A_MOUSE
AccessioniPrimary (citable) accession number: Q9DB34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health