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Q9DB30

- PHKG2_MOUSE

UniProt

Q9DB30 - PHKG2_MOUSE

Protein

Phosphorylase b kinase gamma catalytic chain, liver/testis isoform

Gene

Phkg2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM By similarity.By similarity

    Catalytic activityi

    2 ATP + phosphorylase b = 2 ADP + phosphorylase a.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531ATPPROSITE-ProRule annotation
    Active sitei153 – 1531Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi30 – 389ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphorylase kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycogen biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen metabolism

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.19. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphorylase b kinase gamma catalytic chain, liver/testis isoform (EC:2.7.11.19)
    Short name:
    PHK-gamma-LT
    Short name:
    PHK-gamma-T
    Alternative name(s):
    Phosphorylase kinase subunit gamma-2
    Gene namesi
    Name:Phkg2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1916211. Phkg2.

    Subcellular locationi

    GO - Cellular componenti

    1. phosphorylase kinase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 406406Phosphorylase b kinase gamma catalytic chain, liver/testis isoformPRO_0000086513Add
    BLAST

    Proteomic databases

    PaxDbiQ9DB30.
    PRIDEiQ9DB30.

    PTM databases

    PhosphoSiteiQ9DB30.

    Expressioni

    Gene expression databases

    BgeeiQ9DB30.
    CleanExiMM_PHKG2.
    GenevestigatoriQ9DB30.

    Interactioni

    Subunit structurei

    Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000033086.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DB30.
    SMRiQ9DB30. Positions 10-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 291268Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni306 – 33025Calmodulin-binding (domain-N)By similarityAdd
    BLAST
    Regioni346 – 37025Calmodulin-binding (domain-C)By similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117716.
    HOGENOMiHOG000233016.
    HOVERGENiHBG106193.
    InParanoidiA6H632.
    KOiK00871.
    OMAiHRPPGPF.
    OrthoDBiEOG7JMGDM.
    TreeFamiTF320349.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR002291. Phosph_kin_gamma.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01049. PHOSPHBKNASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9DB30-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLDVGPEDE LPDWAAAKEF YQKYDPKDII GRGVSSVVRR CVHRATGDEF    50
    AVKIMEVSAE RLSLEQLEEV RDATRREMHI LRQVAGHPHI ITLIDSYESS 100
    SFMFLVFDLM RKGELFDYLT EKVALSEKET RSIMRSLLEA VSFLHANNIV 150
    HRDLKPENIL LDDNMQIRLS DFGFSCHLEA GEKLRELCGT PGYLAPEILK 200
    CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL MLRMIMEGQY 250
    QFTSPEWDDR SNTVKDLISK LLQVDPEARL TAEQALQHPF FERCEGSQPW 300
    NLTPRQRFRV AVWTILAAGR VALSSHRLRP LTKNALLRDP YALRPVRRLI 350
    DNCAFRLYGH WVKKGEQQNR AALFQHQPPR LFPIAATELE GDSGAITEDE 400
    ATLVRS 406
    Length:406
    Mass (Da):46,572
    Last modified:July 27, 2011 - v2
    Checksum:i2B12C7F0F8FE054C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti260 – 2601R → C in BAB23926. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK005277 mRNA. Translation: BAB23926.1.
    CH466531 Genomic DNA. Translation: EDL17548.1.
    CH466531 Genomic DNA. Translation: EDL17549.1.
    BC138913 mRNA. Translation: AAI38914.1.
    BC145734 mRNA. Translation: AAI45735.1.
    CCDSiCCDS21869.1.
    RefSeqiNP_081164.2. NM_026888.3.
    XP_006508230.1. XM_006508167.1.
    UniGeneiMm.274473.

    Genome annotation databases

    EnsembliENSMUST00000033086; ENSMUSP00000033086; ENSMUSG00000030815.
    ENSMUST00000121004; ENSMUSP00000113533; ENSMUSG00000030815.
    GeneIDi68961.
    KEGGimmu:68961.
    UCSCiuc009jwc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK005277 mRNA. Translation: BAB23926.1 .
    CH466531 Genomic DNA. Translation: EDL17548.1 .
    CH466531 Genomic DNA. Translation: EDL17549.1 .
    BC138913 mRNA. Translation: AAI38914.1 .
    BC145734 mRNA. Translation: AAI45735.1 .
    CCDSi CCDS21869.1.
    RefSeqi NP_081164.2. NM_026888.3.
    XP_006508230.1. XM_006508167.1.
    UniGenei Mm.274473.

    3D structure databases

    ProteinModelPortali Q9DB30.
    SMRi Q9DB30. Positions 10-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000033086.

    PTM databases

    PhosphoSitei Q9DB30.

    Proteomic databases

    PaxDbi Q9DB30.
    PRIDEi Q9DB30.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033086 ; ENSMUSP00000033086 ; ENSMUSG00000030815 .
    ENSMUST00000121004 ; ENSMUSP00000113533 ; ENSMUSG00000030815 .
    GeneIDi 68961.
    KEGGi mmu:68961.
    UCSCi uc009jwc.2. mouse.

    Organism-specific databases

    CTDi 5261.
    MGIi MGI:1916211. Phkg2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117716.
    HOGENOMi HOG000233016.
    HOVERGENi HBG106193.
    InParanoidi A6H632.
    KOi K00871.
    OMAi HRPPGPF.
    OrthoDBi EOG7JMGDM.
    TreeFami TF320349.

    Enzyme and pathway databases

    BRENDAi 2.7.11.19. 3474.

    Miscellaneous databases

    NextBioi 328293.
    PROi Q9DB30.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9DB30.
    CleanExi MM_PHKG2.
    Genevestigatori Q9DB30.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR002291. Phosph_kin_gamma.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01049. PHOSPHBKNASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiPHKG2_MOUSE
    AccessioniPrimary (citable) accession number: Q9DB30
    Secondary accession number(s): A6H632
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 5, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3