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Protein

Malignant T-cell-amplified sequence 1

Gene

Mcts1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Anti-oncogene that plays a role in cell cycle regulation; decreases cell doubling time and anchorage-dependent growth; shortens the duration of G1 transit time and G1/S transition. When constituvely expressed, increases CDK4 and CDK6 kinases activity and CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex formation. Involved in translation initiation; promotes recruitment of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role as translation enhancer; recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Hyperactivates DNA damage signaling pathway; increased gamma-irradiation-induced phosphorylation of histone H2AX, and induces damage foci formation. Increases the overall number of chromosomal abnormalities such as larger chromosomes formation and multiples chromosomal fusions when overexpressed in gamma-irradiated cells. May play a role in promoting lymphoid tumor development: lymphoid cell lines overexpressing MCTS1 exhibit increased growth rates and display increased protection against apoptosis. May contribute to the pathogenesis and progression of breast cancer via promotion of angiogenesis through the decline of inhibitory THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the process of proteasome degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer cell; Positively regulates phosphorylation of MAPK1 and MAPK3 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Cell cycle, DNA damage, Growth regulation, Protein biosynthesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Malignant T-cell-amplified sequence 1
Short name:
MCT-1
Alternative name(s):
Multiple copies T-cell malignancies 1
Gene namesi
Name:Mcts1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1916245. Mcts1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003447871 – 181Malignant T-cell-amplified sequence 1Add BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81PhosphothreonineBy similarity1
Modified residuei118PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation is critical for stabilization and promotion of cell proliferation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9DB27.
PaxDbiQ9DB27.
PeptideAtlasiQ9DB27.
PRIDEiQ9DB27.

PTM databases

iPTMnetiQ9DB27.
PhosphoSitePlusiQ9DB27.

Expressioni

Gene expression databases

BgeeiENSMUSG00000000355.
GenevisibleiQ9DB27. MM.

Interactioni

Subunit structurei

Interacts (via PUA domain) with DENR.By similarity

Protein-protein interaction databases

IntActiQ9DB27. 1 interactor.
MINTiMINT-4128072.
STRINGi10090.ENSMUSP00000000365.

Structurei

3D structure databases

ProteinModelPortaliQ9DB27.
SMRiQ9DB27.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 171PUAPROSITE-ProRule annotationAdd BLAST80

Domaini

The PUA RNA-binding domain is critical for cap binding, but not sufficient for translation enhancer function. MCT1 N-terminal region is required to enhance translation possibly through interaction with other proteins (By similarity).By similarity

Sequence similaritiesi

Belongs to the MCTS1 family.Curated
Contains 1 PUA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2523. Eukaryota.
COG2016. LUCA.
GeneTreeiENSGT00550000074964.
HOGENOMiHOG000223988.
HOVERGENiHBG105551.
InParanoidiQ9DB27.
KOiK07575.
OMAiNHIMPKK.
OrthoDBiEOG091G0LZ0.
PhylomeDBiQ9DB27.
TreeFamiTF315123.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
InterProiIPR016437. MCT-1/Tma20.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR22798. PTHR22798. 1 hit.
PfamiPF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF005067. Tma_RNA-bind_prd. 1 hit.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DB27-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFKKFDEKEN VSNCIQLKTS VIKGIKNQLL EQFPGIEPWL NQIMPKKDPV
60 70 80 90 100
KIVRCHEHIE ILTVNGELLF FRQREGPFYP TLRLLHKYPF ILPHQQVDKG
110 120 130 140 150
AIKFVLSGAN IMCPGLTSPG AKLYPAAVDT IVAIMAEGKQ HALCVGVMKM
160 170 180
SAEDIEKVNK GIGIENIHYL NDGLWHMKTY K
Length:181
Mass (Da):20,555
Last modified:June 1, 2001 - v1
Checksum:iA8E02C2F992B74BD
GO
Isoform 2 (identifier: Q9DB27-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MFKK → MGKGR

Show »
Length:182
Mass (Da):20,550
Checksum:i0ED704B1C7602954
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0348571 – 4MFKK → MGKGR in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005292 mRNA. Translation: BAB23936.1.
AK087975 mRNA. Translation: BAC40069.1.
AK138385 mRNA. Translation: BAE23639.1.
AL513356, AL845279 Genomic DNA. Translation: CAM17146.1.
AL845279, AL513356 Genomic DNA. Translation: CAM20158.1.
BC010486 mRNA. Translation: AAH10486.1.
CCDSiCCDS30094.1. [Q9DB27-1]
RefSeqiNP_081178.1. NM_026902.3. [Q9DB27-1]
XP_006541598.1. XM_006541535.2. [Q9DB27-2]
UniGeneiMm.262453.

Genome annotation databases

EnsembliENSMUST00000000365; ENSMUSP00000000365; ENSMUSG00000000355. [Q9DB27-1]
GeneIDi68995.
KEGGimmu:68995.
UCSCiuc009tad.1. mouse. [Q9DB27-1]
uc009tae.1. mouse. [Q9DB27-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005292 mRNA. Translation: BAB23936.1.
AK087975 mRNA. Translation: BAC40069.1.
AK138385 mRNA. Translation: BAE23639.1.
AL513356, AL845279 Genomic DNA. Translation: CAM17146.1.
AL845279, AL513356 Genomic DNA. Translation: CAM20158.1.
BC010486 mRNA. Translation: AAH10486.1.
CCDSiCCDS30094.1. [Q9DB27-1]
RefSeqiNP_081178.1. NM_026902.3. [Q9DB27-1]
XP_006541598.1. XM_006541535.2. [Q9DB27-2]
UniGeneiMm.262453.

3D structure databases

ProteinModelPortaliQ9DB27.
SMRiQ9DB27.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DB27. 1 interactor.
MINTiMINT-4128072.
STRINGi10090.ENSMUSP00000000365.

PTM databases

iPTMnetiQ9DB27.
PhosphoSitePlusiQ9DB27.

Proteomic databases

EPDiQ9DB27.
PaxDbiQ9DB27.
PeptideAtlasiQ9DB27.
PRIDEiQ9DB27.

Protocols and materials databases

DNASUi68995.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000365; ENSMUSP00000000365; ENSMUSG00000000355. [Q9DB27-1]
GeneIDi68995.
KEGGimmu:68995.
UCSCiuc009tad.1. mouse. [Q9DB27-1]
uc009tae.1. mouse. [Q9DB27-2]

Organism-specific databases

CTDi28985.
MGIiMGI:1916245. Mcts1.

Phylogenomic databases

eggNOGiKOG2523. Eukaryota.
COG2016. LUCA.
GeneTreeiENSGT00550000074964.
HOGENOMiHOG000223988.
HOVERGENiHBG105551.
InParanoidiQ9DB27.
KOiK07575.
OMAiNHIMPKK.
OrthoDBiEOG091G0LZ0.
PhylomeDBiQ9DB27.
TreeFamiTF315123.

Miscellaneous databases

ChiTaRSiMcts1. mouse.
PROiQ9DB27.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000355.
GenevisibleiQ9DB27. MM.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
InterProiIPR016437. MCT-1/Tma20.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERiPTHR22798. PTHR22798. 1 hit.
PfamiPF01472. PUA. 1 hit.
[Graphical view]
PIRSFiPIRSF005067. Tma_RNA-bind_prd. 1 hit.
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00451. unchar_dom_2. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCTS1_MOUSE
AccessioniPrimary (citable) accession number: Q9DB27
Secondary accession number(s): Q3UUI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.