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Protein

Dolichyl-phosphate beta-glucosyltransferase

Gene

Alg5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

UDP-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-480985. Synthesis of dolichyl-phosphate-glucose.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate beta-glucosyltransferase (EC:2.4.1.117)
Short name:
DolP-glucosyltransferase
Alternative name(s):
Asparagine-linked glycosylation protein 5 homolog
Gene namesi
Name:Alg5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1913498. Alg5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 324LumenalSequence analysisAdd BLAST296

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000590991 – 324Dolichyl-phosphate beta-glucosyltransferaseAdd BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi281N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9DB25.
MaxQBiQ9DB25.
PaxDbiQ9DB25.
PRIDEiQ9DB25.

2D gel databases

REPRODUCTION-2DPAGEQ9DB25.

PTM databases

iPTMnetiQ9DB25.
PhosphoSitePlusiQ9DB25.

Expressioni

Gene expression databases

BgeeiENSMUSG00000036632.
ExpressionAtlasiQ9DB25. baseline and differential.
GenevisibleiQ9DB25. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9DB25. 1 interactor.
MINTiMINT-4087614.
STRINGi10090.ENSMUSP00000035879.

Structurei

3D structure databases

ProteinModelPortaliQ9DB25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2977. Eukaryota.
COG0463. LUCA.
GeneTreeiENSGT00550000074941.
HOVERGENiHBG024330.
InParanoidiQ9DB25.
KOiK00729.
OMAiFIRLRYM.
OrthoDBiEOG091G0C84.
TreeFamiTF314844.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DB25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLLLQLLG LGVALAAAAL ILVSIVAFIT ATKMPPCYQH EEEKFFLNAK
60 70 80 90 100
GQKEALPSIW DSPTKQLSVV VPSYNEEKRL PVMMDEALNY LEKRQKHDCT
110 120 130 140 150
FTYEVIVVDD GSEDQTSKVA LKYCQKYGSD KVRVITLVRN RGKGGAVRMG
160 170 180 190 200
VFSSRGEKIL MADADGATKF PDVEKLEKGL SDLQPWPEQM AIACGSRAHL
210 220 230 240 250
EKESIAQRSY FRTFLMYGFH FLVWFLCVKG IRDTQCGFKL LTREAAARTF
260 270 280 290 300
SSLHIERWAF DVELLYIAQC LQIPIAEVAV NWTEIEGSKL VPFWSWLQMG
310 320
KDLLFIRLRY LTGAWRLKQT RKAS
Length:324
Mass (Da):36,791
Last modified:June 1, 2001 - v1
Checksum:i83203C070D571523
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005296 mRNA. Translation: BAB23938.1.
BC027160 mRNA. Translation: AAH27160.1.
CCDSiCCDS17353.1.
RefSeqiNP_079718.1. NM_025442.3.
UniGeneiMm.144218.

Genome annotation databases

EnsembliENSMUST00000044567; ENSMUSP00000035879; ENSMUSG00000036632.
GeneIDi66248.
KEGGimmu:66248.
UCSCiuc008pft.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005296 mRNA. Translation: BAB23938.1.
BC027160 mRNA. Translation: AAH27160.1.
CCDSiCCDS17353.1.
RefSeqiNP_079718.1. NM_025442.3.
UniGeneiMm.144218.

3D structure databases

ProteinModelPortaliQ9DB25.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9DB25. 1 interactor.
MINTiMINT-4087614.
STRINGi10090.ENSMUSP00000035879.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

PTM databases

iPTMnetiQ9DB25.
PhosphoSitePlusiQ9DB25.

2D gel databases

REPRODUCTION-2DPAGEQ9DB25.

Proteomic databases

EPDiQ9DB25.
MaxQBiQ9DB25.
PaxDbiQ9DB25.
PRIDEiQ9DB25.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044567; ENSMUSP00000035879; ENSMUSG00000036632.
GeneIDi66248.
KEGGimmu:66248.
UCSCiuc008pft.1. mouse.

Organism-specific databases

CTDi29880.
MGIiMGI:1913498. Alg5.

Phylogenomic databases

eggNOGiKOG2977. Eukaryota.
COG0463. LUCA.
GeneTreeiENSGT00550000074941.
HOVERGENiHBG024330.
InParanoidiQ9DB25.
KOiK00729.
OMAiFIRLRYM.
OrthoDBiEOG091G0C84.
TreeFamiTF314844.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-480985. Synthesis of dolichyl-phosphate-glucose.

Miscellaneous databases

PROiQ9DB25.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036632.
ExpressionAtlasiQ9DB25. baseline and differential.
GenevisibleiQ9DB25. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiALG5_MOUSE
AccessioniPrimary (citable) accession number: Q9DB25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.