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Protein

Alpha-soluble NSF attachment protein

Gene

Napa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. Together with GNA12 promotes CDH5 localization to plasma membrane.By similarity

GO - Molecular functioni

GO - Biological processi

  • apical protein localization Source: MGI
  • brain development Source: MGI
  • intracellular protein transport Source: MGI
  • membrane fusion Source: GO_Central
  • neuron differentiation Source: MGI
  • positive regulation of ATPase activity Source: Ensembl
  • regulation of synaptic vesicle priming Source: MGI
  • SNARE complex disassembly Source: MGI
  • synaptic transmission, glutamatergic Source: MGI

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.
R-MMU-6807878. COPI-mediated anterograde transport.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-MMU-6811438. Intra-Golgi traffic.
R-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-soluble NSF attachment protein
Short name:
SNAP-alpha
Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein alpha
Gene namesi
Name:Napa
Synonyms:Snapa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:104563. Napa.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190571 – 295Alpha-soluble NSF attachment proteinAdd BLAST295

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei26PhosphoserineCombined sources1
Modified residuei29PhosphoserineBy similarity1
Modified residuei195PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9DB05.
MaxQBiQ9DB05.
PaxDbiQ9DB05.
PeptideAtlasiQ9DB05.
PRIDEiQ9DB05.

2D gel databases

REPRODUCTION-2DPAGEiQ9DB05.

PTM databases

iPTMnetiQ9DB05.
PhosphoSitePlusiQ9DB05.
SwissPalmiQ9DB05.

Expressioni

Gene expression databases

BgeeiENSMUSG00000006024.
ExpressionAtlasiQ9DB05. baseline and differential.
GenevisibleiQ9DB05. MM.

Interactioni

Subunit structurei

Interacts with PRKCABP, and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2 (By similarity). Found in a complex with VAMP8 (By similarity). Component of a SNARE-like complex that contains at least ZW10, USE1L, RINT1, STX18 and NAPA/SNAP-alpha (By similarity). Interacts with VTI1A (PubMed:9705316). Interacts with STX12 (By similarity). Interacts with GNA12 (via N-terminus); the interaction promotes CDH5 localization to plasma membrane (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi223847. 3 interactors.
IntActiQ9DB05. 5 interactors.
MINTiMINT-1857852.
STRINGi10090.ENSMUSP00000006181.

Structurei

3D structure databases

ProteinModelPortaliQ9DB05.
SMRiQ9DB05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SNAP family.Curated

Phylogenomic databases

eggNOGiKOG1586. Eukaryota.
ENOG410XPQ6. LUCA.
GeneTreeiENSGT00390000005826.
HOGENOMiHOG000165015.
HOVERGENiHBG001325.
InParanoidiQ9DB05.
KOiK15296.
OMAiMADNEQK.
OrthoDBiEOG091G0KSY.
PhylomeDBiQ9DB05.
TreeFamiTF316547.

Family and domain databases

CDDicd15832. SNAP. 1 hit.
Gene3Di1.25.40.10. 1 hit.
InterProiView protein in InterPro
IPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom.
PANTHERiPTHR13768. PTHR13768. 1 hit.
PRINTSiPR00448. NSFATTACHMNT.
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DB05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNSGKQAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN
60 70 80 90 100
MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA
110 120 130 140 150
INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDVEK AIAHYEQSAD
160 170 180 190 200
YYKGEESNSS ANKCLLKVAG YAAQLEQYQK AIDIYEQVGT SAMDSPLLKY
210 220 230 240 250
SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA
260 270 280 290
HEEQNVDSYT EAVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
Length:295
Mass (Da):33,190
Last modified:June 1, 2001 - v1
Checksum:i30D099B598992AA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005372 mRNA. Translation: BAB23981.1.
AK050623 mRNA. Translation: BAC34348.1.
AK136689 mRNA. Translation: BAE23100.1.
AK171998 mRNA. Translation: BAE42767.1.
BC004804 mRNA. Translation: AAH04804.1.
CCDSiCCDS20843.1.
RefSeqiNP_080174.1. NM_025898.3.
UniGeneiMm.104540.

Genome annotation databases

EnsembliENSMUST00000006181; ENSMUSP00000006181; ENSMUSG00000006024.
GeneIDi108124.
KEGGimmu:108124.
UCSCiuc009fgw.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiSNAA_MOUSE
AccessioniPrimary (citable) accession number: Q9DB05
Secondary accession number(s): Q543I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: June 1, 2001
Last modified: September 27, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families