Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Abscission/NoCut checkpoint regulator

Gene

Zfyve19

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 58FYVE-typePROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

Lipid-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Abscission/NoCut checkpoint regulator
Short name:
ANCHR
Alternative name(s):
Zinc finger FYVE domain-containing protein 19
Gene namesi
Name:Zfyve19
Synonyms:Anchr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919258. Zfyve19.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000987191 – 389Abscission/NoCut checkpoint regulatorAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69PhosphoserineBy similarity1
Cross-linki132Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei219PhosphoserineBy similarity1
Modified residuei280PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9DAZ9.
MaxQBiQ9DAZ9.
PaxDbiQ9DAZ9.
PeptideAtlasiQ9DAZ9.
PRIDEiQ9DAZ9.

PTM databases

iPTMnetiQ9DAZ9.
PhosphoSitePlusiQ9DAZ9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000068580.
CleanExiMM_ZFYVE19.
ExpressionAtlasiQ9DAZ9. baseline and differential.
GenevisibleiQ9DAZ9. MM.

Interactioni

Subunit structurei

Interacts (via MIM1-B) with VPS4A; interaction takes place at the midbody ring following cytokinesis checkpoint activation.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087636.

Structurei

Secondary structure

1389
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Beta strandi14 – 16Combined sources3
Beta strandi18 – 20Combined sources3
Beta strandi22 – 24Combined sources3
Beta strandi27 – 29Combined sources3
Turni30 – 32Combined sources3
Beta strandi35 – 39Combined sources5
Turni40 – 43Combined sources4
Beta strandi44 – 49Combined sources6
Helixi51 – 59Combined sources9
Beta strandi66 – 68Combined sources3
Turni340 – 342Combined sources3
Beta strandi348 – 350Combined sources3
Turni351 – 354Combined sources4
Beta strandi355 – 358Combined sources4
Helixi362 – 366Combined sources5
Turni367 – 369Combined sources3
Turni372 – 374Combined sources3
Beta strandi383 – 386Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WFKNMR-A1-75[»]
2D8VNMR-A336-389[»]
ProteinModelPortaliQ9DAZ9.
SMRiQ9DAZ9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9DAZ9.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili226 – 261Sequence analysisAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi99 – 112MIM1-AAdd BLAST14
Motifi252 – 265MIM1-BAdd BLAST14

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi332 – 335Poly-Glu4
Compositional biasi339 – 362Cys-richAdd BLAST24

Domaini

The FYVE-type zinc finger mediates binding to phosphatidylinositol-3-phosphate (PtdIns3P).By similarity
The MIM1-B motif mediates interaction with VPS4A.By similarity

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 58FYVE-typePROSITE-ProRule annotationAdd BLAST58

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1818. Eukaryota.
ENOG410XNRF. LUCA.
GeneTreeiENSGT00390000016108.
HOGENOMiHOG000231992.
HOVERGENiHBG057281.
InParanoidiQ9DAZ9.
OMAiRCGNTQQ.
OrthoDBiEOG091G0F4F.
TreeFamiTF317652.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9DAZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESRCYGCAV KFTLFKKEYG CKNCGRAFCN GCLSFSALVP RAGNTQQKVC
60 70 80 90 100
KQCHTILTRG SSDNASKWSP PQNYKKRVAA LEAKKKSSTS HSQSLTHKDQ
110 120 130 140 150
AIAERLARLR QENKPKSVPS QAEIEARLAA LKDEVQGPIP STQEMEDRLA
160 170 180 190 200
ALQGRVPPSH TVRPAHQAPD TRTQAQQTQD LLTQLTAEVA IDENCQPRAS
210 220 230 240 250
ASLQNDLNKG AARSQRTNSQ GQASQSLEEE KYKLLAEAAV ELQEENTRQE
260 270 280 290 300
RILALAKRLA VLKGQDPSRV TLQDYHLPDS DEDEETAIQR VMQQLTEEAA
310 320 330 340 350
LDEASGFNIP EKPAPGSRAQ PCKAEMEGPQ AEEEELPWCC ICNEDATLRC
360 370 380
AGCDGDLYCA RCFREGHDNF DLKEHQTSPY HPRRPCQEH
Length:389
Mass (Da):43,272
Last modified:July 27, 2011 - v2
Checksum:i13AA752764AB17A1
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti64N → S in strain: FVB/N. 1 Publication1
Natural varianti91H → Q in strain: FVB/N. 1 Publication1
Natural varianti94S → G in strain: FVB/N. 1 Publication1
Natural varianti157P → T in strain: C57BL/6. 1 Publication1
Natural varianti164P → L in strain: FVB/N. 1 Publication1
Natural varianti229E → A in strain: FVB/N. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005386 mRNA. Translation: BAB23993.1.
AL929318, AL772264 Genomic DNA. Translation: CAM25334.1.
AL772264, AL929318 Genomic DNA. Translation: CAM45941.1.
BC018420 mRNA. Translation: AAH18420.1.
CCDSiCCDS16595.1.
RefSeqiNP_082330.2. NM_028054.3.
UniGeneiMm.386807.

Genome annotation databases

EnsembliENSMUST00000090174; ENSMUSP00000087636; ENSMUSG00000068580.
GeneIDi72008.
KEGGimmu:72008.
UCSCiuc008lti.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005386 mRNA. Translation: BAB23993.1.
AL929318, AL772264 Genomic DNA. Translation: CAM25334.1.
AL772264, AL929318 Genomic DNA. Translation: CAM45941.1.
BC018420 mRNA. Translation: AAH18420.1.
CCDSiCCDS16595.1.
RefSeqiNP_082330.2. NM_028054.3.
UniGeneiMm.386807.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WFKNMR-A1-75[»]
2D8VNMR-A336-389[»]
ProteinModelPortaliQ9DAZ9.
SMRiQ9DAZ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087636.

PTM databases

iPTMnetiQ9DAZ9.
PhosphoSitePlusiQ9DAZ9.

Proteomic databases

EPDiQ9DAZ9.
MaxQBiQ9DAZ9.
PaxDbiQ9DAZ9.
PeptideAtlasiQ9DAZ9.
PRIDEiQ9DAZ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090174; ENSMUSP00000087636; ENSMUSG00000068580.
GeneIDi72008.
KEGGimmu:72008.
UCSCiuc008lti.2. mouse.

Organism-specific databases

CTDi84936.
MGIiMGI:1919258. Zfyve19.

Phylogenomic databases

eggNOGiKOG1818. Eukaryota.
ENOG410XNRF. LUCA.
GeneTreeiENSGT00390000016108.
HOGENOMiHOG000231992.
HOVERGENiHBG057281.
InParanoidiQ9DAZ9.
OMAiRCGNTQQ.
OrthoDBiEOG091G0F4F.
TreeFamiTF317652.

Miscellaneous databases

EvolutionaryTraceiQ9DAZ9.
PROiQ9DAZ9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000068580.
CleanExiMM_ZFYVE19.
ExpressionAtlasiQ9DAZ9. baseline and differential.
GenevisibleiQ9DAZ9. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANCHR_MOUSE
AccessioniPrimary (citable) accession number: Q9DAZ9
Secondary accession number(s): A2AV55, Q8VCV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.