ID   PRP4_MOUSE              Reviewed;         521 AA.
AC   Q9DAW6;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   27-MAR-2024, entry version 173.
DE   RecName: Full=U4/U6 small nuclear ribonucleoprotein Prp4;
DE   AltName: Full=U4/U6 snRNP 60 kDa protein;
DE   AltName: Full=WD splicing factor Prp4;
GN   Name=Prpf4;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J, and NOD; TISSUE=Placenta, Spleen, and Thymus;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [4]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-26, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Embryonic fibroblast;
RX   PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA   Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA   Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT   "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT   pathways.";
RL   Mol. Cell 50:919-930(2013).
CC   -!- FUNCTION: Plays a role in pre-mRNA splicing as component of the U4/U6-
CC       U5 tri-snRNP complex that is involved in spliceosome assembly, and as
CC       component of the precatalytic spliceosome (spliceosome B complex).
CC       {ECO:0000250|UniProtKB:O43172}.
CC   -!- SUBUNIT: Component of the precatalytic spliceosome (spliceosome B
CC       complex) (By similarity). Component of the U4/U6-U5 tri-snRNP complex,
CC       a building block of the precatalytic spliceosome (spliceosome B
CC       complex) (By similarity). The U4/U6-U5 tri-snRNP complex is composed of
CC       the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8,
CC       PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3,
CC       SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and
CC       USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 (By
CC       similarity). Interacts directly with PRPF18, PPIH and PRPF3 (By
CC       similarity). Part of a heteromeric complex containing PPIH, PRPF3 and
CC       PRPF4 that is stable in the absence of RNA (By similarity). Interacts
CC       with ERCC6 (By similarity). {ECO:0000250|UniProtKB:O43172}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43172}. Nucleus
CC       speckle {ECO:0000250|UniProtKB:O43172}.
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DR   EMBL; AK005464; BAB24055.1; -; mRNA.
DR   EMBL; AK089838; BAC40976.1; -; mRNA.
DR   EMBL; AK166912; BAE39112.1; -; mRNA.
DR   EMBL; AK167216; BAE39342.1; -; mRNA.
DR   EMBL; AK169532; BAE41214.1; -; mRNA.
DR   EMBL; BC106089; AAI06090.1; -; mRNA.
DR   CCDS; CCDS18239.1; -.
DR   RefSeq; NP_081573.1; NM_027297.3.
DR   AlphaFoldDB; Q9DAW6; -.
DR   SMR; Q9DAW6; -.
DR   BioGRID; 213838; 14.
DR   IntAct; Q9DAW6; 1.
DR   MINT; Q9DAW6; -.
DR   STRING; 10090.ENSMUSP00000081572; -.
DR   GlyGen; Q9DAW6; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q9DAW6; -.
DR   PhosphoSitePlus; Q9DAW6; -.
DR   SwissPalm; Q9DAW6; -.
DR   EPD; Q9DAW6; -.
DR   jPOST; Q9DAW6; -.
DR   MaxQB; Q9DAW6; -.
DR   PaxDb; 10090-ENSMUSP00000081572; -.
DR   ProteomicsDB; 291792; -.
DR   Pumba; Q9DAW6; -.
DR   Antibodypedia; 15276; 158 antibodies from 23 providers.
DR   DNASU; 70052; -.
DR   Ensembl; ENSMUST00000084524.4; ENSMUSP00000081572.4; ENSMUSG00000066148.4.
DR   GeneID; 70052; -.
DR   KEGG; mmu:70052; -.
DR   UCSC; uc008ter.1; mouse.
DR   AGR; MGI:1917302; -.
DR   CTD; 9128; -.
DR   MGI; MGI:1917302; Prpf4.
DR   VEuPathDB; HostDB:ENSMUSG00000066148; -.
DR   eggNOG; KOG0272; Eukaryota.
DR   GeneTree; ENSGT00940000156006; -.
DR   HOGENOM; CLU_000288_57_20_1; -.
DR   InParanoid; Q9DAW6; -.
DR   OMA; LNEPICY; -.
DR   OrthoDB; 5476798at2759; -.
DR   PhylomeDB; Q9DAW6; -.
DR   TreeFam; TF314922; -.
DR   Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR   BioGRID-ORCS; 70052; 28 hits in 79 CRISPR screens.
DR   ChiTaRS; Prpf4; mouse.
DR   PRO; PR:Q9DAW6; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   RNAct; Q9DAW6; Protein.
DR   Bgee; ENSMUSG00000066148; Expressed in manus and 216 other cell types or tissues.
DR   ExpressionAtlas; Q9DAW6; baseline and differential.
DR   GO; GO:0015030; C:Cajal body; ISO:MGI.
DR   GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISO:MGI.
DR   GO; GO:0097525; C:spliceosomal snRNP complex; ISS:UniProtKB.
DR   GO; GO:0071005; C:U2-type precatalytic spliceosome; ISO:MGI.
DR   GO; GO:0071001; C:U4/U6 snRNP; ISS:UniProtKB.
DR   GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; ISO:MGI.
DR   GO; GO:0030621; F:U4 snRNA binding; IBA:GO_Central.
DR   GO; GO:0017070; F:U6 snRNA binding; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISO:MGI.
DR   CDD; cd00200; WD40; 1.
DR   Gene3D; 4.10.280.110; Pre-mRNA processing factor 4 domain; 1.
DR   Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 3.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR014906; PRP4-like.
DR   InterPro; IPR036285; PRP4-like_sf.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR001680; WD40_rpt.
DR   PANTHER; PTHR19846:SF5; U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP4; 1.
DR   PANTHER; PTHR19846; WD40 REPEAT PROTEIN; 1.
DR   Pfam; PF08799; PRP4; 1.
DR   Pfam; PF00400; WD40; 7.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00500; SFM; 1.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF158230; PRP4-like; 1.
DR   SUPFAM; SSF50978; WD40 repeat-like; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 6.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
DR   Genevisible; Q9DAW6; MM.
PE   1: Evidence at protein level;
KW   Acetylation; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Repeat; Spliceosome; WD repeat.
FT   CHAIN           1..521
FT                   /note="U4/U6 small nuclear ribonucleoprotein Prp4"
FT                   /id="PRO_0000051150"
FT   REPEAT          228..267
FT                   /note="WD 1"
FT   REPEAT          270..317
FT                   /note="WD 2"
FT   REPEAT          320..359
FT                   /note="WD 3"
FT   REPEAT          362..401
FT                   /note="WD 4"
FT   REPEAT          404..443
FT                   /note="WD 5"
FT   REPEAT          446..486
FT                   /note="WD 6"
FT   REPEAT          489..521
FT                   /note="WD 7"
FT   MOD_RES         26
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0007744|PubMed:23806337"
SQ   SEQUENCE   521 AA;  58370 MW;  CDE80E76D591CC89 CRC64;
     MASSRASSTT TKTKAPDDLV APVVKKPHIY YGSLEEKERE RLAKGESGIL GKEGLKAGIE
     AGNINITSGE VFEIEEHISE RQAEVLAEFE RRKRARQINV STDDSEVKAC LRALGEPITL
     FGEGPAERRE RLRNILSVVG TDALKKTKKD DEKSKKSKEE YQQTWYHEGP NSLKVARLWI
     ANYSLPRAMK RLEEARLHKE IPETTRTSQM QELHKSLRSL NNFCSQIGDD RPISYCHFSP
     NSKMLATACW SGLCKLWSVP DCSLLHTLRG HNTNVGAIVF HPKSTVSLDQ KDVNLASCAA
     DGSVKLWSLD SDEPVADIEG HTVRVARVMW HPSGRFLGTT CYDRSWRLWD LEAQEEILHQ
     EGHSMGVYDI AFHQDGSLAG TGGLDAFGRV WDLRTGRCIM FLEGHLKEIY GINFSPNGYH
     IATGSGDNTC KVWDLRQRRC VYTIPAHQNL VTGVKFEPIH GDFLLTGAYD NTAKIWTHPG
     WSPLKTLAGH EGKVMGLDIS SDGQLIATCS YDRTFKLWMA E
//