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Protein

U4/U6 small nuclear ribonucleoprotein Prp4

Gene

Prpf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein Prp4
Alternative name(s):
U4/U6 snRNP 60 kDa protein
WD splicing factor Prp4
Gene namesi
Name:Prpf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917302. Prpf4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511501 – 521U4/U6 small nuclear ribonucleoprotein Prp4Add BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9DAW6.
MaxQBiQ9DAW6.
PaxDbiQ9DAW6.
PRIDEiQ9DAW6.

PTM databases

iPTMnetiQ9DAW6.
PhosphoSitePlusiQ9DAW6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000066148.
CleanExiMM_PRPF4.
ExpressionAtlasiQ9DAW6. baseline and differential.
GenevisibleiQ9DAW6. MM.

Interactioni

Subunit structurei

Interacts directly with PRPF18, PPIH and PRPF3. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (By similarity).By similarity

Protein-protein interaction databases

BioGridi213838. 2 interactors.
IntActiQ9DAW6. 1 interactor.
MINTiMINT-4127974.
STRINGi10090.ENSMUSP00000081572.

Structurei

3D structure databases

ProteinModelPortaliQ9DAW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati228 – 267WD 1Add BLAST40
Repeati270 – 317WD 2Add BLAST48
Repeati320 – 359WD 3Add BLAST40
Repeati362 – 401WD 4Add BLAST40
Repeati404 – 443WD 5Add BLAST40
Repeati446 – 486WD 6Add BLAST41
Repeati489 – 521WD 7Add BLAST33

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0272. Eukaryota.
ENOG410XNP8. LUCA.
GeneTreeiENSGT00900000140925.
HOGENOMiHOG000157615.
HOVERGENiHBG030892.
InParanoidiQ9DAW6.
KOiK12662.
OMAiDSSWRLW.
OrthoDBiEOG091G0HXT.
PhylomeDBiQ9DAW6.
TreeFamiTF314922.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiView protein in InterPro
IPR020472. G-protein_beta_WD-40_rep.
IPR027106. Prp4.
IPR014906. PRP4-like.
IPR036285. PRP4-like_sf.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PANTHERiPTHR19846. PTHR19846. 1 hit.
PfamiView protein in Pfam
PF08799. PRP4. 1 hit.
PF00400. WD40. 6 hits.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00500. SFM. 1 hit.
SM00320. WD40. 7 hits.
SUPFAMiSSF158230. SSF158230. 1 hit.
SSF50978. SSF50978. 1 hit.
PROSITEiView protein in PROSITE
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DAW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSRASSTT TKTKAPDDLV APVVKKPHIY YGSLEEKERE RLAKGESGIL
60 70 80 90 100
GKEGLKAGIE AGNINITSGE VFEIEEHISE RQAEVLAEFE RRKRARQINV
110 120 130 140 150
STDDSEVKAC LRALGEPITL FGEGPAERRE RLRNILSVVG TDALKKTKKD
160 170 180 190 200
DEKSKKSKEE YQQTWYHEGP NSLKVARLWI ANYSLPRAMK RLEEARLHKE
210 220 230 240 250
IPETTRTSQM QELHKSLRSL NNFCSQIGDD RPISYCHFSP NSKMLATACW
260 270 280 290 300
SGLCKLWSVP DCSLLHTLRG HNTNVGAIVF HPKSTVSLDQ KDVNLASCAA
310 320 330 340 350
DGSVKLWSLD SDEPVADIEG HTVRVARVMW HPSGRFLGTT CYDRSWRLWD
360 370 380 390 400
LEAQEEILHQ EGHSMGVYDI AFHQDGSLAG TGGLDAFGRV WDLRTGRCIM
410 420 430 440 450
FLEGHLKEIY GINFSPNGYH IATGSGDNTC KVWDLRQRRC VYTIPAHQNL
460 470 480 490 500
VTGVKFEPIH GDFLLTGAYD NTAKIWTHPG WSPLKTLAGH EGKVMGLDIS
510 520
SDGQLIATCS YDRTFKLWMA E
Length:521
Mass (Da):58,370
Last modified:June 1, 2001 - v1
Checksum:iCDE80E76D591CC89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005464 mRNA. Translation: BAB24055.1.
AK089838 mRNA. Translation: BAC40976.1.
AK166912 mRNA. Translation: BAE39112.1.
AK167216 mRNA. Translation: BAE39342.1.
AK169532 mRNA. Translation: BAE41214.1.
BC106089 mRNA. Translation: AAI06090.1.
CCDSiCCDS18239.1.
RefSeqiNP_081573.1. NM_027297.3.
UniGeneiMm.30660.

Genome annotation databases

EnsembliENSMUST00000084524; ENSMUSP00000081572; ENSMUSG00000066148.
GeneIDi70052.
KEGGimmu:70052.
UCSCiuc008ter.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiPRP4_MOUSE
AccessioniPrimary (citable) accession number: Q9DAW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2001
Last modified: November 22, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot