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Q9DAW6 (PRP4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U4/U6 small nuclear ribonucleoprotein Prp4
Alternative name(s):
U4/U6 snRNP 60 kDa protein
WD splicing factor Prp4
Gene names
Name:Prpf4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome By similarity.

Subunit structure

Interacts directly with PRPF18, PPIH and PRPF3. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39 By similarity.

Subcellular location

Nucleus speckle By similarity. Note: Colocalizes with spliceosomal snRNPs By similarity.

Sequence similarities

Contains 7 WD repeats.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentNucleus
Spliceosome
   DomainRepeat
WD repeat
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA splicing

Inferred from electronic annotation. Source: UniProtKB-KW

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentCajal body

Inferred from electronic annotation. Source: Ensembl

U4/U6 snRNP

Inferred from electronic annotation. Source: Ensembl

nuclear speck

Inferred from electronic annotation. Source: UniProtKB-SubCell

spliceosomal complex

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 521521U4/U6 small nuclear ribonucleoprotein Prp4
PRO_0000051150

Regions

Repeat228 – 26740WD 1
Repeat270 – 31748WD 2
Repeat320 – 35940WD 3
Repeat362 – 40140WD 4
Repeat404 – 44340WD 5
Repeat446 – 48641WD 6
Repeat489 – 52133WD 7

Amino acid modifications

Modified residue261N6-acetyllysine Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9DAW6 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: CDE80E76D591CC89

FASTA52158,370
        10         20         30         40         50         60 
MASSRASSTT TKTKAPDDLV APVVKKPHIY YGSLEEKERE RLAKGESGIL GKEGLKAGIE 

        70         80         90        100        110        120 
AGNINITSGE VFEIEEHISE RQAEVLAEFE RRKRARQINV STDDSEVKAC LRALGEPITL 

       130        140        150        160        170        180 
FGEGPAERRE RLRNILSVVG TDALKKTKKD DEKSKKSKEE YQQTWYHEGP NSLKVARLWI 

       190        200        210        220        230        240 
ANYSLPRAMK RLEEARLHKE IPETTRTSQM QELHKSLRSL NNFCSQIGDD RPISYCHFSP 

       250        260        270        280        290        300 
NSKMLATACW SGLCKLWSVP DCSLLHTLRG HNTNVGAIVF HPKSTVSLDQ KDVNLASCAA 

       310        320        330        340        350        360 
DGSVKLWSLD SDEPVADIEG HTVRVARVMW HPSGRFLGTT CYDRSWRLWD LEAQEEILHQ 

       370        380        390        400        410        420 
EGHSMGVYDI AFHQDGSLAG TGGLDAFGRV WDLRTGRCIM FLEGHLKEIY GINFSPNGYH 

       430        440        450        460        470        480 
IATGSGDNTC KVWDLRQRRC VYTIPAHQNL VTGVKFEPIH GDFLLTGAYD NTAKIWTHPG 

       490        500        510        520 
WSPLKTLAGH EGKVMGLDIS SDGQLIATCS YDRTFKLWMA E 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Placenta, Spleen and Thymus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[3]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-26, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK005464 mRNA. Translation: BAB24055.1.
AK089838 mRNA. Translation: BAC40976.1.
AK166912 mRNA. Translation: BAE39112.1.
AK167216 mRNA. Translation: BAE39342.1.
AK169532 mRNA. Translation: BAE41214.1.
BC106089 mRNA. Translation: AAI06090.1.
RefSeqNP_081573.1. NM_027297.3.
UniGeneMm.30660.

3D structure databases

ProteinModelPortalQ9DAW6.
SMRQ9DAW6. Positions 106-136, 238-519.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid213838. 1 interaction.
IntActQ9DAW6. 1 interaction.
MINTMINT-4127974.
STRING10090.ENSMUSP00000081572.

PTM databases

PhosphoSiteQ9DAW6.

Proteomic databases

PaxDbQ9DAW6.
PRIDEQ9DAW6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000084524; ENSMUSP00000081572; ENSMUSG00000066148.
GeneID70052.
KEGGmmu:70052.
UCSCuc008ter.1. mouse.

Organism-specific databases

CTD9128.
MGIMGI:1917302. Prpf4.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00740000115057.
HOGENOMHOG000157615.
HOVERGENHBG030892.
InParanoidQ9DAW6.
KOK12662.
OMAHPSRQAY.
OrthoDBEOG7P5T0Z.
PhylomeDBQ9DAW6.
TreeFamTF314922.

Gene expression databases

ArrayExpressQ9DAW6.
BgeeQ9DAW6.
CleanExMM_PRPF4.
GenevestigatorQ9DAW6.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR027106. Prp4.
IPR014906. PRP4-like.
IPR003648. SFM.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERPTHR19846. PTHR19846. 1 hit.
PfamPF08799. PRP4. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00500. SFM. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPRPF4. mouse.
NextBio330897.
PROQ9DAW6.
SOURCESearch...

Entry information

Entry namePRP4_MOUSE
AccessionPrimary (citable) accession number: Q9DAW6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot