Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitochondrial tRNA-specific 2-thiouridylase 1

Gene

Trmu

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.By similarity

Catalytic activityi

A [protein]-S-sulfanyl-L-cysteine + 5-taurinomethyluridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 5-taurinomethyl-2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei101NucleophileBy similarity1
Binding sitei126ATP; via amide nitrogenBy similarity1
Active sitei222Cysteine persulfide intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BRENDAi2.1.1.61. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial tRNA-specific 2-thiouridylase 1 (EC:2.8.1.14By similarity)
Gene namesi
Name:Trmu
Synonyms:Mtu1, Trmt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1919276. Trmu.

Subcellular locationi

  • Mitochondrion 1 Publication

GO - Cellular componenti

  • mitochondrion Source: UniProtKB
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217091 – 417Mitochondrial tRNA-specific 2-thiouridylase 1Add BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi101 ↔ 222By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ9DAT5.
MaxQBiQ9DAT5.
PaxDbiQ9DAT5.
PeptideAtlasiQ9DAT5.
PRIDEiQ9DAT5.

PTM databases

PhosphoSitePlusiQ9DAT5.

Expressioni

Tissue specificityi

Widely expressed but most abundant in tissues with high metabolic rate including heart, liver and brain. Expression is low in spleen, testis, lung and skeletal muscle. Also expressed in inner ear.1 Publication

Gene expression databases

BgeeiENSMUSG00000022386.
ExpressionAtlasiQ9DAT5. baseline and differential.
GenevisibleiQ9DAT5. MM.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei127Interaction with tRNABy similarity1
Sitei267Interaction with tRNABy similarity1
Sitei367Interaction with tRNABy similarity1

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023019.

Structurei

3D structure databases

ProteinModelPortaliQ9DAT5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 98Interaction with target base in tRNABy similarity3
Regioni171 – 173Interaction with tRNABy similarity3
Regioni334 – 335Interaction with tRNABy similarity2

Sequence similaritiesi

Belongs to the MnmA/TRMU family.Curated

Phylogenomic databases

eggNOGiKOG2805. Eukaryota.
COG0482. LUCA.
GeneTreeiENSGT00390000014323.
HOGENOMiHOG000218046.
HOVERGENiHBG108157.
InParanoidiQ9DAT5.
KOiK00566.
OMAiLHKINFA.
OrthoDBiEOG091G0BKX.
PhylomeDBiQ9DAT5.
TreeFamiTF105611.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9DAT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALRHVVCA LSGGVDSAVA ALLLRRRGYQ VTGVFMKNWD SLDEQGVCAA
60 70 80 90 100
DKDCEDAYKV CQILDIPFHQ VSYVKEYWND VFSDFLNEYE KGRTPNPDIN
110 120 130 140 150
CNKHIKFSCF YHYAVDNLGA DAVATGHYAR TSLEDEEVFE QKHTKKPDGL
160 170 180 190 200
FRNRFEVRNP VKLLQAADSF KDQTFFLSQV SQDALRRTIF PLGELTKDFV
210 220 230 240 250
KKIAAENSLH HVLQKRESMG ICFIGKRNLE HFLLQYLQPR PGKFVSIEDN
260 270 280 290 300
TVLGTHKGWF LYTLGQRAKI SGLREPWYVV EKDGTKGDVL VAPRVDHPAL
310 320 330 340 350
YRDLLRTNRV HWIAEEPPAA LVRDKMMECH FRFRHQMALV PCVLTLNQDG
360 370 380 390 400
TVWVTAVKAV RGLALGQFAV FYKGEECLGS GKILRLGPSA YTLQKGKNRT
410
RVAPEASSDS PGLHPTS
Length:417
Mass (Da):47,240
Last modified:June 1, 2001 - v1
Checksum:i8B4F0E6F461953CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY349617 mRNA. Translation: AAQ55218.1.
AK005541 mRNA. Translation: BAB24110.1.
AK043532 mRNA. Translation: BAC31570.1.
AK044059 mRNA. Translation: BAC31758.1.
BC061026 mRNA. Translation: AAH61026.1.
CCDSiCCDS27726.1.
RefSeqiNP_082339.1. NM_028063.2.
UniGeneiMm.158211.

Genome annotation databases

EnsembliENSMUST00000023019; ENSMUSP00000023019; ENSMUSG00000022386.
GeneIDi72026.
KEGGimmu:72026.
UCSCiuc007xdr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY349617 mRNA. Translation: AAQ55218.1.
AK005541 mRNA. Translation: BAB24110.1.
AK043532 mRNA. Translation: BAC31570.1.
AK044059 mRNA. Translation: BAC31758.1.
BC061026 mRNA. Translation: AAH61026.1.
CCDSiCCDS27726.1.
RefSeqiNP_082339.1. NM_028063.2.
UniGeneiMm.158211.

3D structure databases

ProteinModelPortaliQ9DAT5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023019.

PTM databases

PhosphoSitePlusiQ9DAT5.

Proteomic databases

EPDiQ9DAT5.
MaxQBiQ9DAT5.
PaxDbiQ9DAT5.
PeptideAtlasiQ9DAT5.
PRIDEiQ9DAT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023019; ENSMUSP00000023019; ENSMUSG00000022386.
GeneIDi72026.
KEGGimmu:72026.
UCSCiuc007xdr.1. mouse.

Organism-specific databases

CTDi55687.
MGIiMGI:1919276. Trmu.

Phylogenomic databases

eggNOGiKOG2805. Eukaryota.
COG0482. LUCA.
GeneTreeiENSGT00390000014323.
HOGENOMiHOG000218046.
HOVERGENiHBG108157.
InParanoidiQ9DAT5.
KOiK00566.
OMAiLHKINFA.
OrthoDBiEOG091G0BKX.
PhylomeDBiQ9DAT5.
TreeFamiTF105611.

Enzyme and pathway databases

BRENDAi2.1.1.61. 3474.

Miscellaneous databases

PROiQ9DAT5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022386.
ExpressionAtlasiQ9DAT5. baseline and differential.
GenevisibleiQ9DAT5. MM.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTU1_MOUSE
AccessioniPrimary (citable) accession number: Q9DAT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

During the reaction, ATP is used to activate the C2 atom of U34 by adenylation. After this, the persulfide sulfur on the catalytic cysteine is transferred to the C2 atom of the wobble base (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards the activated C2 atom on U34. Subsequently, a transient disulfide bond is formed between the two active site cysteine residues (By similarity).By similarity

Caution

Was originally thought to be a 5-methylaminomethyl-2-methyltransferase involved in tRNA modification.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.