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Protein

Mitochondrial tRNA-specific 2-thiouridylase 1

Gene

Trmu

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.By similarity

Miscellaneous

During the reaction, ATP is used to activate the C2 atom of U34 by adenylation. After this, the persulfide sulfur on the catalytic cysteine is transferred to the C2 atom of the wobble base (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards the activated C2 atom on U34. Subsequently, a transient disulfide bond is formed between the two active site cysteine residues (By similarity).By similarity

Caution

Was originally thought to be a 5-methylaminomethyl-2-methyltransferase involved in tRNA modification.1 Publication

Catalytic activityi

A [protein]-S-sulfanyl-L-cysteine + 5-taurinomethyluridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 5-taurinomethyl-2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei101NucleophileBy similarity1
Binding sitei126ATP; via amide nitrogenBy similarity1
Active sitei222Cysteine persulfide intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.1.1.61 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial tRNA-specific 2-thiouridylase 1 (EC:2.8.1.14By similarity)
Gene namesi
Name:Trmu
Synonyms:Mtu1, Trmt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1919276 Trmu

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217091 – 417Mitochondrial tRNA-specific 2-thiouridylase 1Add BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi101 ↔ 222By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ9DAT5
MaxQBiQ9DAT5
PaxDbiQ9DAT5
PeptideAtlasiQ9DAT5
PRIDEiQ9DAT5

PTM databases

iPTMnetiQ9DAT5
PhosphoSitePlusiQ9DAT5

Expressioni

Tissue specificityi

Widely expressed but most abundant in tissues with high metabolic rate including heart, liver and brain. Expression is low in spleen, testis, lung and skeletal muscle. Also expressed in inner ear.1 Publication

Gene expression databases

BgeeiENSMUSG00000022386
ExpressionAtlasiQ9DAT5 baseline and differential
GenevisibleiQ9DAT5 MM

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei127Interaction with tRNABy similarity1
Sitei267Interaction with tRNABy similarity1
Sitei367Interaction with tRNABy similarity1

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023019

Structurei

3D structure databases

ProteinModelPortaliQ9DAT5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 98Interaction with target base in tRNABy similarity3
Regioni171 – 173Interaction with tRNABy similarity3
Regioni334 – 335Interaction with tRNABy similarity2

Sequence similaritiesi

Belongs to the MnmA/TRMU family.Curated

Phylogenomic databases

eggNOGiKOG2805 Eukaryota
COG0482 LUCA
GeneTreeiENSGT00390000014323
HOGENOMiHOG000218046
HOVERGENiHBG108157
InParanoidiQ9DAT5
KOiK21027
OMAiAVCTGHY
OrthoDBiEOG091G0BKX
PhylomeDBiQ9DAT5
TreeFamiTF105611

Family and domain databases

CDDicd01998 tRNA_Me_trans, 1 hit
Gene3Di2.30.30.280, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00144 tRNA_thiouridyl_MnmA, 1 hit
InterProiView protein in InterPro
IPR023382 Adenine_a_hdrlase_dom
IPR014729 Rossmann-like_a/b/a_fold
IPR004506 tRNA-specific_2-thiouridylase
PANTHERiPTHR11933 PTHR11933, 1 hit
TIGRFAMsiTIGR00420 trmU, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DAT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALRHVVCA LSGGVDSAVA ALLLRRRGYQ VTGVFMKNWD SLDEQGVCAA
60 70 80 90 100
DKDCEDAYKV CQILDIPFHQ VSYVKEYWND VFSDFLNEYE KGRTPNPDIN
110 120 130 140 150
CNKHIKFSCF YHYAVDNLGA DAVATGHYAR TSLEDEEVFE QKHTKKPDGL
160 170 180 190 200
FRNRFEVRNP VKLLQAADSF KDQTFFLSQV SQDALRRTIF PLGELTKDFV
210 220 230 240 250
KKIAAENSLH HVLQKRESMG ICFIGKRNLE HFLLQYLQPR PGKFVSIEDN
260 270 280 290 300
TVLGTHKGWF LYTLGQRAKI SGLREPWYVV EKDGTKGDVL VAPRVDHPAL
310 320 330 340 350
YRDLLRTNRV HWIAEEPPAA LVRDKMMECH FRFRHQMALV PCVLTLNQDG
360 370 380 390 400
TVWVTAVKAV RGLALGQFAV FYKGEECLGS GKILRLGPSA YTLQKGKNRT
410
RVAPEASSDS PGLHPTS
Length:417
Mass (Da):47,240
Last modified:June 1, 2001 - v1
Checksum:i8B4F0E6F461953CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY349617 mRNA Translation: AAQ55218.1
AK005541 mRNA Translation: BAB24110.1
AK043532 mRNA Translation: BAC31570.1
AK044059 mRNA Translation: BAC31758.1
BC061026 mRNA Translation: AAH61026.1
CCDSiCCDS27726.1
RefSeqiNP_082339.1, NM_028063.2
UniGeneiMm.158211

Genome annotation databases

EnsembliENSMUST00000023019; ENSMUSP00000023019; ENSMUSG00000022386
GeneIDi72026
KEGGimmu:72026
UCSCiuc007xdr.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMTU1_MOUSE
AccessioniPrimary (citable) accession number: Q9DAT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health