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Protein

Dynein light chain roadblock-type 2

Gene

Dynlrb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein light chain roadblock-type 2
Alternative name(s):
Dynein light chain 2B, cytoplasmic
Gene namesi
Name:Dynlrb2
Synonyms:Dncl2b, Dnlc2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1922715. Dynlrb2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • cytoplasmic dynein complex Source: UniProtKB
  • extracellular exosome Source: MGI
  • microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 9695Dynein light chain roadblock-type 2PRO_0000220959Add
BLAST

Proteomic databases

MaxQBiQ9DAJ5.
PaxDbiQ9DAJ5.
PRIDEiQ9DAJ5.

PTM databases

PhosphoSiteiQ9DAJ5.

Expressioni

Gene expression databases

BgeeiQ9DAJ5.
CleanExiMM_DYNLRB2.
GenevisibleiQ9DAJ5. MM.

Interactioni

Subunit structurei

Homodimer (Probable). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1I1 and DYNC1I2. Self-associates. Interacts with DYNLRB1 (By similarity).By similarityCurated

Protein-protein interaction databases

BioGridi217500. 1 interaction.
STRINGi10090.ENSMUSP00000077301.

Structurei

3D structure databases

ProteinModelPortaliQ9DAJ5.
SMRiQ9DAJ5. Positions 3-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GAMAD family.Curated

Phylogenomic databases

eggNOGiKOG4115. Eukaryota.
ENOG4111NDV. LUCA.
GeneTreeiENSGT00390000011067.
HOGENOMiHOG000232033.
HOVERGENiHBG058598.
InParanoidiQ9DAJ5.
KOiK10419.
OMAiAHKGVIG.
OrthoDBiEOG7X0VKJ.
PhylomeDBiQ9DAJ5.
TreeFamiTF315165.

Family and domain databases

InterProiIPR016561. DYNLRB1/2.
IPR004942. Roadblock/LAMTOR2_dom.
[Graphical view]
PfamiPF03259. Robl_LC7. 1 hit.
[Graphical view]
PIRSFiPIRSF009998. DLC7. 1 hit.
SMARTiSM00960. Robl_LC7. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DAJ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTEVEETLKR IQSHKGVIGT MVVNAEGIPI RTTLDNSTTV QYAGLLHQLT
60 70 80 90
MKAKSTVRDI DPQNDLTFLR IRSKKHEIMV APDKEYLLIV IQNPCE
Length:96
Mass (Da):10,876
Last modified:June 1, 2001 - v1
Checksum:i5C446A9D0E815775
GO
Isoform 2 (identifier: Q9DAJ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     83-96: DKEYLLIVIQNPCE → EPM

Note: No experimental confirmation available.
Show »
Length:85
Mass (Da):9,574
Checksum:i0FBE9771068A49EB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei83 – 9614DKEYL…QNPCE → EPM in isoform 2. 1 PublicationVSP_007238Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005789 mRNA. Translation: BAB24240.1.
BC048623 mRNA. Translation: AAH48623.1.
CCDSiCCDS40487.1. [Q9DAJ5-1]
RefSeqiNP_083573.1. NM_029297.1. [Q9DAJ5-1]
UniGeneiMm.23114.

Genome annotation databases

EnsembliENSMUST00000078170; ENSMUSP00000077301; ENSMUSG00000034467. [Q9DAJ5-1]
GeneIDi75465.
KEGGimmu:75465.
UCSCiuc009nog.1. mouse. [Q9DAJ5-1]
uc009noh.1. mouse. [Q9DAJ5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005789 mRNA. Translation: BAB24240.1.
BC048623 mRNA. Translation: AAH48623.1.
CCDSiCCDS40487.1. [Q9DAJ5-1]
RefSeqiNP_083573.1. NM_029297.1. [Q9DAJ5-1]
UniGeneiMm.23114.

3D structure databases

ProteinModelPortaliQ9DAJ5.
SMRiQ9DAJ5. Positions 3-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217500. 1 interaction.
STRINGi10090.ENSMUSP00000077301.

PTM databases

PhosphoSiteiQ9DAJ5.

Proteomic databases

MaxQBiQ9DAJ5.
PaxDbiQ9DAJ5.
PRIDEiQ9DAJ5.

Protocols and materials databases

DNASUi75465.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078170; ENSMUSP00000077301; ENSMUSG00000034467. [Q9DAJ5-1]
GeneIDi75465.
KEGGimmu:75465.
UCSCiuc009nog.1. mouse. [Q9DAJ5-1]
uc009noh.1. mouse. [Q9DAJ5-2]

Organism-specific databases

CTDi83657.
MGIiMGI:1922715. Dynlrb2.

Phylogenomic databases

eggNOGiKOG4115. Eukaryota.
ENOG4111NDV. LUCA.
GeneTreeiENSGT00390000011067.
HOGENOMiHOG000232033.
HOVERGENiHBG058598.
InParanoidiQ9DAJ5.
KOiK10419.
OMAiAHKGVIG.
OrthoDBiEOG7X0VKJ.
PhylomeDBiQ9DAJ5.
TreeFamiTF315165.

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Miscellaneous databases

PROiQ9DAJ5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9DAJ5.
CleanExiMM_DYNLRB2.
GenevisibleiQ9DAJ5. MM.

Family and domain databases

InterProiIPR016561. DYNLRB1/2.
IPR004942. Roadblock/LAMTOR2_dom.
[Graphical view]
PfamiPF03259. Robl_LC7. 1 hit.
[Graphical view]
PIRSFiPIRSF009998. DLC7. 1 hit.
SMARTiSM00960. Robl_LC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  3. "The Roadblock light chains are ubiquitous components of cytoplasmic dynein that form homo- and heterodimers."
    Nikulina K., Patel-King R.S., Takebe S., Pfister K.K., King S.M.
    Cell Motil. Cytoskeleton 57:233-245(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SELF-ASSOCIATION, INTERACTION WITH DYNLRB1, IDENTIFICATION IN THE CYTOPLASMIC DYNEIN I COMPLEX.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiDLRB2_MOUSE
AccessioniPrimary (citable) accession number: Q9DAJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.