Q9DAG9 (PHF7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PHD finger protein 7

Alternative name(s):
Testis development protein NYD-SP6

Gene names

Name:

Phf7

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in spermatogenesis.
Subcellular location	Nucleus By similarity.
Tissue specificity	Highly expressed in Sertoli cells in testis. Ref.3
Developmental stage	Expression levels increase during the first 4 weeks after birth and remain constant during the following 3 weeks. Ref.3
Sequence similarities	Contains 1 PHD-type zinc finger. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9DAG9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9DAG9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     228-266: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 307

307

PHD finger protein 7

PRO_0000055997

Regions

Zinc finger

97 – 145

49

PHD-type; atypical

Zinc finger

160 – 208

49

RING-type; degenerate

Natural variations

Alternative sequence

228 – 266

39

Missing in isoform 2.

VSP_013491

Secondary structure

1

.

.

.

.

.

.

.

.

.

307

Helix Strand Turn

Details...

Beta strand

263 – 266

4

Beta strand

274 – 276

3

Helix

284 – 286

3

Turn

298 – 300

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: B838B4CE2ED20BBA	FASTA	307	35,381
	10 20 30 40 50 60 MKTLKEKNKH PRLRKTIRTK KVTQRKLSSS PVCLLCLQEP GDPEKLGEFL QKDNLCVHYF 70 80 90 100 110 120 CLILSSRLPQ KGQPNRGLHG FMPEDIKREA VRASKKICFV CKKKGAAIRC QNDQCVQNFH 130 140 150 160 170 180 LPCGQERGCL SQFFGEYKSY CRKHRPTQNI HQGSLGEESC VLCCENLSRT SVENIQSPCC 190 200 210 220 230 240 SQAIYHRKCI QKYAHTSAKH FFKCPQCNNR EEFPQEMLRM GIHIPDRDAA WELEPGAFSE 250 260 270 280 290 300 LYQRYRHCDA PICLYEQGRD SFEDEGRWRL ILCATCGSHG THRDCSSLRP NSKKWECNEC LPASTTS
	Isoform 2 [UniParc]. Checksum: 393D959DBBED305F	FASTA	268	30,831
	10 20 30 40 50 60 MKTLKEKNKH PRLRKTIRTK KVTQRKLSSS PVCLLCLQEP GDPEKLGEFL QKDNLCVHYF 70 80 90 100 110 120 CLILSSRLPQ KGQPNRGLHG FMPEDIKREA VRASKKICFV CKKKGAAIRC QNDQCVQNFH 130 140 150 160 170 180 LPCGQERGCL SQFFGEYKSY CRKHRPTQNI HQGSLGEESC VLCCENLSRT SVENIQSPCC 190 200 210 220 230 240 SQAIYHRKCI QKYAHTSAKH FFKCPQCNNR EEFPQEMLRM GIHIPDRRWR LILCATCGSH 250 260 GTHRDCSSLR PNSKKWECNE CLPASTTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N-3. Tissue: Mammary gland.
[3]	"NYD-SP6, a novel gene potentially involved in regulating testicular development/spermatogenesis." Xiao J., Xu M., Li J., Chang Chan H., Lin M., Zhu H., Zhang W., Zhou Z., Zhao B., Sha J.H. Biochem. Biophys. Res. Commun. 291:101-110(2002) [PubMed: 11829468] [Abstract] Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Tissue: Testis.
[4]	"Solution structure of PHD domain in PHD finger protein 7." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 232-304.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK005852 mRNA. Translation: BAB24275.1.
BC057174 mRNA. Translation: AAH57174.1.

IPI

IPI00118609.
IPI00461882.

RefSeq

NP_082225.1. NM_027949.1.

UniGene

Mm.5348.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WEQ	NMR	-	A	232-303	[»]

ProteinModelPortal

Q9DAG9.

SMR

Q9DAG9. Positions 96-148, 155-210, 232-307.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9DAG9.

Proteomic databases

PRIDE

Q9DAG9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000022459; ENSMUSP00000022459; ENSMUSG00000021902.
ENSMUST00000112051; ENSMUSP00000107682; ENSMUSG00000021902.

GeneID

71838.

KEGG

mmu:71838.

UCSC

uc007sxg.1. mouse.

Organism-specific databases

CTD

51533.

MGI

MGI:1919088. Phf7.

Phylogenomic databases

GeneTree

ENSGT00390000005246.

HOGENOM

HBG506600.

HOVERGEN

HBG053588.

InParanoid

Q9DAG9.

OMA

CLYEQGR.

OrthoDB

EOG46WZ8H.

PhylomeDB

Q9DAG9.

Gene expression databases

ArrayExpress

Q9DAG9.

Bgee

Q9DAG9.

CleanEx

MM_PHF7.

Genevestigator

Q9DAG9.

GermOnline

ENSMUSG00000021902. Mus musculus.

Family and domain databases

InterPro

IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 2 hits.

SMART

SM00249. PHD. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]

SUPFAM

SSF57903. FYVE_PHD_ZnF. 1 hit.

PROSITE

PS01359. ZF_PHD_1. False negative.
PS50016. ZF_PHD_2. False negative.
PS00518. ZF_RING_1. False negative.
PS50089. ZF_RING_2. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

334684.

SOURCE

Search...

Entry information

Entry name

PHF7_MOUSE

Accession

Primary (citable) accession number: Q9DAG9
Secondary accession number(s): Q6PG81

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2001
Last modified:	September 21, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program