ID   PROF3_MOUSE             Reviewed;         137 AA.
AC   Q9DAD6;
DT   29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   24-JAN-2024, entry version 141.
DE   RecName: Full=Profilin-3;
DE   AltName: Full=Profilin III;
GN   Name=Pfn3;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC   STRAIN=129/Sv;
RX   PubMed=11867228; DOI=10.1016/s0378-1119(01)00855-1;
RA   Braun A., Aszodi A., Hellebrand H., Berna A., Fassler R., Brandau O.;
RT   "Genomic organization of profilin-III and evidence for a transcript
RT   expressed exclusively in testis.";
RL   Gene 283:219-225(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   SUBCELLULAR LOCATION, AND INTERACTION WITH ACTRT3.
RX   PubMed=18692047; DOI=10.1016/j.febslet.2008.07.058;
RA   Hara Y., Yamagata K., Oguchi K., Baba T.;
RT   "Nuclear localization of profilin III-ArpM1 complex in mouse
RT   spermiogenesis.";
RL   FEBS Lett. 582:2998-3004(2008).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Binds to actin and affects the structure of the cytoskeleton.
CC       Binds to poly-L-proline, phosphatidylinositol 3-phosphate (PtdIns(3)P),
CC       phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and
CC       phosphatidylinositol 4-phosphate (PtdIns(4)P). Slightly reduces actin
CC       polymerization. May be involved in spermatogenesis. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with ACTRT3. {ECO:0000269|PubMed:18692047}.
CC   -!- INTERACTION:
CC       Q9DAD6; Q8BXF8: Actrt3; NbExp=3; IntAct=EBI-6480328, EBI-6480313;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}. Nucleus
CC       {ECO:0000269|PubMed:18692047}.
CC   -!- TISSUE SPECIFICITY: Testis specific. {ECO:0000269|PubMed:11867228}.
CC   -!- SIMILARITY: Belongs to the profilin family. {ECO:0000305}.
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DR   EMBL; AF422809; AAL65908.1; -; Genomic_DNA.
DR   EMBL; AK005930; BAB24322.1; -; mRNA.
DR   EMBL; BC048629; AAH48629.1; -; mRNA.
DR   CCDS; CCDS26545.1; -.
DR   RefSeq; NP_083579.1; NM_029303.2.
DR   AlphaFoldDB; Q9DAD6; -.
DR   SMR; Q9DAD6; -.
DR   IntAct; Q9DAD6; 1.
DR   MINT; Q9DAD6; -.
DR   STRING; 10090.ENSMUSP00000054053; -.
DR   PhosphoSitePlus; Q9DAD6; -.
DR   SwissPalm; Q9DAD6; -.
DR   PaxDb; 10090-ENSMUSP00000054053; -.
DR   ProteomicsDB; 291563; -.
DR   Pumba; Q9DAD6; -.
DR   Antibodypedia; 48836; 23 antibodies from 5 providers.
DR   DNASU; 75477; -.
DR   Ensembl; ENSMUST00000054146.5; ENSMUSP00000054053.4; ENSMUSG00000044444.7.
DR   GeneID; 75477; -.
DR   KEGG; mmu:75477; -.
DR   UCSC; uc007qqu.1; mouse.
DR   AGR; MGI:2178800; -.
DR   CTD; 345456; -.
DR   MGI; MGI:2178800; Pfn3.
DR   VEuPathDB; HostDB:ENSMUSG00000044444; -.
DR   eggNOG; ENOG502S2A3; Eukaryota.
DR   GeneTree; ENSGT00940000153664; -.
DR   HOGENOM; CLU_123405_1_0_1; -.
DR   InParanoid; Q9DAD6; -.
DR   OMA; VCVGHTP; -.
DR   OrthoDB; 2965090at2759; -.
DR   PhylomeDB; Q9DAD6; -.
DR   TreeFam; TF331744; -.
DR   BioGRID-ORCS; 75477; 2 hits in 77 CRISPR screens.
DR   ChiTaRS; Pfn3; mouse.
DR   PRO; PR:Q9DAD6; -.
DR   Proteomes; UP000000589; Chromosome 13.
DR   RNAct; Q9DAD6; Protein.
DR   Bgee; ENSMUSG00000044444; Expressed in seminiferous tubule of testis and 20 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; IBA:GO_Central.
DR   GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR   GO; GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
DR   GO; GO:0032233; P:positive regulation of actin filament bundle assembly; IBA:GO_Central.
DR   GO; GO:0030833; P:regulation of actin filament polymerization; IBA:GO_Central.
DR   CDD; cd00148; PROF; 1.
DR   Gene3D; 3.30.450.30; Dynein light chain 2a, cytoplasmic; 1.
DR   InterPro; IPR048278; PFN.
DR   InterPro; IPR005455; PFN_euk.
DR   InterPro; IPR036140; PFN_sf.
DR   InterPro; IPR005454; Profilin1/2/3_vertebrate.
DR   PANTHER; PTHR13936; PROFILIN; 1.
DR   PANTHER; PTHR13936:SF2; PROFILIN-3; 1.
DR   Pfam; PF00235; Profilin; 1.
DR   PRINTS; PR01639; PROFILINMAML.
DR   SMART; SM00392; PROF; 1.
DR   SUPFAM; SSF55770; Profilin (actin-binding protein); 1.
DR   Genevisible; Q9DAD6; MM.
PE   1: Evidence at protein level;
KW   Actin-binding; Cytoplasm; Cytoskeleton; Lipid-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..137
FT                   /note="Profilin-3"
FT                   /id="PRO_0000199580"
SQ   SEQUENCE   137 AA;  14697 MW;  939E617834F71BC8 CRC64;
     MSDWKGYISA VLRDQRIDDV AIVGHSDNRC VWASRPGGLL AAISPQEVGV LTGPDRHTFL
     QTGLSVAGRR CCVIRDYLLA EGDGVLDART KGLDGRAICV GHTPRALLVL MGRRGVHGGI
     LNKTVHDLIG GLREQCS
//