ID   PO5F2_MOUSE             Reviewed;         329 AA.
AC   Q9DAC9; Q8BVS2;
DT   01-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   08-NOV-2023, entry version 134.
DE   RecName: Full=POU domain, class 5, transcription factor 2;
DE   AltName: Full=Sperm 1 POU domain transcription factor;
DE            Short=SPRM-1;
GN   Name=Pou5f2; Synonyms=Sprm1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=7908264; DOI=10.1111/j.1432-1033.1994.tb18676.x;
RA   Wey E., Lyons G.E., Schaefer B.W.;
RT   "A human POU domain gene, mPOU, is expressed in developing brain and
RT   specific adult tissues.";
RL   Eur. J. Biochem. 220:753-762(1994).
CC   -!- FUNCTION: Transcription factor that binds preferentially to the octamer
CC       motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic
CC       events that are required for terminal differentiation of male germ cell
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC       ECO:0000255|PROSITE-ProRule:PRU00530}.
CC   -!- TISSUE SPECIFICITY: In adult brain, expressed in the olfactory bulb,
CC       becoming specifically concentrated in the mitral cell layer. Also found
CC       in the pyramidal cell layer of the hippocampus, in the granule cell
CC       layer of the cerebellum and in the cortex.
CC       {ECO:0000269|PubMed:7908264}.
CC   -!- DEVELOPMENTAL STAGE: Low expression levels observed at 12 dpc in neural
CC       tube and brain. On days 15.5 and 16.5, expression levels increased and
CC       became localized specifically to the forebrain, with highest levels
CC       found in the laminar region of the forebrain cortex. Also expressed in
CC       the mitral region of the olfactory bulb. {ECO:0000269|PubMed:7908264}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-5
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AK005948; BAB24332.1; -; mRNA.
DR   EMBL; AK076753; BAC36466.1; -; mRNA.
DR   CCDS; CCDS56895.1; -.
DR   RefSeq; NP_083591.1; NM_029315.1.
DR   AlphaFoldDB; Q9DAC9; -.
DR   SMR; Q9DAC9; -.
DR   STRING; 10090.ENSMUSP00000135597; -.
DR   PhosphoSitePlus; Q9DAC9; -.
DR   MaxQB; Q9DAC9; -.
DR   PaxDb; 10090-ENSMUSP00000135597; -.
DR   ProteomicsDB; 289650; -.
DR   GeneID; 75507; -.
DR   KEGG; mmu:75507; -.
DR   UCSC; uc011zcd.1; mouse.
DR   AGR; MGI:1922757; -.
DR   CTD; 134187; -.
DR   MGI; MGI:1922757; Pou5f2.
DR   eggNOG; KOG3802; Eukaryota.
DR   InParanoid; Q9DAC9; -.
DR   OrthoDB; 4250502at2759; -.
DR   PhylomeDB; Q9DAC9; -.
DR   TreeFam; TF316413; -.
DR   BioGRID-ORCS; 75507; 0 hits in 75 CRISPR screens.
DR   PRO; PR:Q9DAC9; -.
DR   Proteomes; UP000000589; Unplaced.
DR   RNAct; Q9DAC9; Protein.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   PANTHER; PTHR11636; POU DOMAIN; 1.
DR   PANTHER; PTHR11636:SF14; POU DOMAIN, CLASS 5, TRANSCRIPTION FACTOR 2; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..329
FT                   /note="POU domain, class 5, transcription factor 2"
FT                   /id="PRO_0000100757"
FT   DOMAIN          107..181
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        199..258
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        292
FT                   /note="T -> P (in Ref. 1; BAC36466)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   329 AA;  36411 MW;  A882639979987F31 CRC64;
     MAGRRSSNVF PLSGNSGGGL EVDTPTWLSS QAATSRLMVR PSMGPGICPG PEVWGVPLGS
     SPYEFRGGIA PYRACEARAW SQSSSEDTCP GPYIALRYMP NLALPEDVSA IQKEMEQLAK
     ELRQKRMTLG YSQADVGFAV GAMFGKVLSQ TTICRFEAQQ LSLANMWKLR PLLKMWLEEV
     DEKNLLGICR MEMILEQARK RRRASRERRI GSNLEKLFLQ CPEPTPQQIS YIAGRLRLQK
     DLVQVWFSNR SQMGSWPTND TSRREDVGAT GSPFPGPPVC FPMAPGLHFD FTHYEGSCLT
     PLYSSTPFPV RGALLSAPTT TLGLPRLSS
//