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Q9DAC9 (PO5F2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 5, transcription factor 2
Alternative name(s):
Sperm 1 POU domain transcription factor
Short name=SPRM-1
Gene names
Name:Pou5f2
Synonyms:Sprm1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that binds preferentially to the octamer motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic events that are required for terminal differentiation of male germ cell By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

In adult brain, expressed in the olfactory bulb, becoming specifically concentrated in the mitral cell layer. Also found in the pyramidal cell layer of the hippocampus, in the granule cell layer of the cerebellum and in the cortex. Ref.2

Developmental stage

Low expression levels observed at 12 dpc in neural tube and brain. On days 15.5 and 16.5, expression levels increased and became localized specifically to the forebrain, with highest levels found in the laminar region of the forebrain cortex. Also expressed in the mitral region of the olfactory bulb. Ref.2

Sequence similarities

Belongs to the POU transcription factor family. Class-5 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsequence-specific DNA binding

Inferred from direct assay PubMed 7902581. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329POU domain, class 5, transcription factor 2
PRO_0000100757

Regions

Domain107 – 18175POU-specific
DNA binding199 – 25860Homeobox

Experimental info

Sequence conflict2921T → P in BAC36466. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9DAC9 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: A882639979987F31

FASTA32936,411
        10         20         30         40         50         60 
MAGRRSSNVF PLSGNSGGGL EVDTPTWLSS QAATSRLMVR PSMGPGICPG PEVWGVPLGS 

        70         80         90        100        110        120 
SPYEFRGGIA PYRACEARAW SQSSSEDTCP GPYIALRYMP NLALPEDVSA IQKEMEQLAK 

       130        140        150        160        170        180 
ELRQKRMTLG YSQADVGFAV GAMFGKVLSQ TTICRFEAQQ LSLANMWKLR PLLKMWLEEV 

       190        200        210        220        230        240 
DEKNLLGICR MEMILEQARK RRRASRERRI GSNLEKLFLQ CPEPTPQQIS YIAGRLRLQK 

       250        260        270        280        290        300 
DLVQVWFSNR SQMGSWPTND TSRREDVGAT GSPFPGPPVC FPMAPGLHFD FTHYEGSCLT 

       310        320 
PLYSSTPFPV RGALLSAPTT TLGLPRLSS 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[2]"A human POU domain gene, mPOU, is expressed in developing brain and specific adult tissues."
Wey E., Lyons G.E., Schaefer B.W.
Eur. J. Biochem. 220:753-762(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK005948 mRNA. Translation: BAB24332.1.
AK076753 mRNA. Translation: BAC36466.1.
CCDSCCDS56895.1.
RefSeqNP_083591.1. NM_029315.1.
UniGeneMm.443255.

3D structure databases

ProteinModelPortalQ9DAC9.
SMRQ9DAC9. Positions 107-254.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9DAC9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000175955; ENSMUSP00000135597; ENSMUSG00000093668.
GeneID75507.
KEGGmmu:75507.
UCSCuc011zcd.1. mouse.

Organism-specific databases

CTD134187.
MGIMGI:1922757. Pou5f2.

Phylogenomic databases

GeneTreeENSGT00750000117482.
HOVERGENHBG057998.
OrthoDBEOG7MKW6H.
PhylomeDBQ9DAC9.
TreeFamTF316413.

Gene expression databases

BgeeQ9DAC9.
GenevestigatorQ9DAC9.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio343200.
PROQ9DAC9.
SOURCESearch...

Entry information

Entry namePO5F2_MOUSE
AccessionPrimary (citable) accession number: Q9DAC9
Secondary accession number(s): Q8BVS2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot