Q9DAA6 (EXOS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified July 9, 2014. Version 98. History...
Names and origin
|Protein names||Recommended name:|
Exosome complex component CSL4
Exosome component 1
|Organism||Mus musculus (Mouse) [Reference proteome]|
|Taxonomic identifier||10090 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus|
|Sequence length||195 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC1 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC6 and EXOSC8 By similarity.
Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core (Exo-9) complex which is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits specifically containing the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and peripheral S1 domain-containing components EXOSC1, EXOSC2 and EXOSC3 located on the top of the ring structure. Interacts with EXOSC5, EXOSC7 and EXOSC10 By similarity. Interacts with DDX60 By similarity.
Belongs to the CSL4 family.
Contains 1 S1 motif domain.
|Biological process||rRNA processing|
|Coding sequence diversity||Alternative splicing|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
Inferred from electronic annotation. Source: UniProtKB-KW
Inferred from electronic annotation. Source: UniProtKB-SubCellexosome (RNase complex)
Inferred from sequence or structural similarity. Source: UniProtKBnucleolus
Inferred from sequence or structural similarity. Source: UniProtKB
Inferred from electronic annotation. Source: UniProtKB-KWprotein binding
|Complete GO annotation...|
|This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]|
|Isoform 1 (identifier: Q9DAA6-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Isoform 2 (identifier: Q9DAA6-2) |
The sequence of this isoform differs from the canonical sequence as follows:
|Note: No experimental confirmation available.|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 195||195||Exosome complex component CSL4||PRO_0000087128|
|Domain||66 – 147||82||S1 motif|
Amino acid modifications
|Modified residue||21||1||Phosphoserine By similarity|
|Alternative sequence||76 – 116||41||Missing in isoform 2.||VSP_004176|
|||"The transcriptional landscape of the mammalian genome."|
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
|||"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."|
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
|+||Additional computationally mapped references.|
|AK002930 mRNA. Translation: BAB22465.1.|
AK006018 mRNA. Translation: BAB24368.1.
BC024423 mRNA. Translation: AAH24423.1.
|RefSeq||NP_001158033.1. NM_001164561.1. [Q9DAA6-2]|
NP_079920.1. NM_025644.4. [Q9DAA6-1]
3D structure databases
|SMR||Q9DAA6. Positions 6-185. |
Protein-protein interaction databases
|BioGrid||211569. 2 interactions.|
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSMUST00000075280; ENSMUSP00000074756; ENSMUSG00000034321. [Q9DAA6-1]|
ENSMUST00000112123; ENSMUSP00000107751; ENSMUSG00000034321. [Q9DAA6-2]
|UCSC||uc008hmm.2. mouse. [Q9DAA6-1]|
uc008hmn.2. mouse. [Q9DAA6-2]
|MGI||MGI:1913833. Exosc1. |
Gene expression databases
Family and domain databases
|InterPro||IPR019495. EXOSC1. |
|Pfam||PF14382. ECR1_N. 1 hit. |
PF10447. EXOSC1. 1 hit.
|SMART||SM00316. S1. 1 hit. |
|SUPFAM||SSF50249. SSF50249. 1 hit. |
|Accession||Primary (citable) accession number: Q9DAA6|
Secondary accession number(s): Q9DCB9
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|