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Protein

Dipeptidase 3

Gene

Dpep3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable metalloprotease which hydrolyzes cystinyl-bis-glycine. May be involved in meiosis.1 Publication

Catalytic activityi

Hydrolysis of dipeptides.PROSITE-ProRule annotation

Cofactori

Zn2+PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by L-penicillamine.1 Publication

Kineticsi

  1. KM=2.5 mM for cystinyl-bis-glycine1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi94 – 941Zinc 1; catalyticPROSITE-ProRule annotation
    Metal bindingi96 – 961Zinc 1; catalyticPROSITE-ProRule annotation
    Metal bindingi193 – 1931Zinc 1; catalyticPROSITE-ProRule annotation
    Metal bindingi193 – 1931Zinc 2; catalyticPROSITE-ProRule annotation
    Metal bindingi266 – 2661Zinc 2; catalyticPROSITE-ProRule annotation
    Metal bindingi287 – 2871Zinc 2; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    GO - Biological processi

    • male meiosis Source: UniProtKB
    • proteolysis Source: MGI
    Complete GO annotation...

    Keywords - Molecular functioni

    Dipeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Biological processi

    Meiosis

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM19.011.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dipeptidase 3 (EC:3.4.13.19)
    Alternative name(s):
    Membrane-bound dipeptidase 3
    Short name:
    MBD-3
    Protein expressed in male leptotene and zygotene spermatocytes 136
    Short name:
    MLZ-136
    Gene namesi
    Name:Dpep3
    Synonyms:Mbd3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:1919104. Dpep3.

    Subcellular locationi

    • Membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

    GO - Cellular componenti

    • anchored component of membrane Source: UniProtKB-KW
    • membrane Source: MGI
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3535Sequence AnalysisAdd
    BLAST
    Chaini36 – 462427Dipeptidase 3PRO_0000231613Add
    BLAST
    Propeptidei463 – 49331Removed in mature formSequence AnalysisPRO_0000231614Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi143 ↔ 222PROSITE-ProRule annotation
    Disulfide bondi294 ↔ 326PROSITE-ProRule annotation
    Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi431 – 431InterchainPROSITE-ProRule annotation
    Lipidationi462 – 4621GPI-anchor amidated serineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ9DA79.
    PRIDEiQ9DA79.

    PTM databases

    PhosphoSiteiQ9DA79.

    Expressioni

    Tissue specificityi

    Expressed in testis but not ovary.2 Publications

    Developmental stagei

    expressed in ovary and testis at E15.5.1 Publication

    Gene expression databases

    BgeeiQ9DA79.
    CleanExiMM_DPEP3.
    MM_MBD3.
    GenevisibleiQ9DA79. MM.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.PROSITE-ProRule annotation

    Protein-protein interaction databases

    IntActiQ9DA79. 1 interaction.
    MINTiMINT-8174108.
    STRINGi10090.ENSMUSP00000034371.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DA79.
    SMRiQ9DA79. Positions 77-434.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M19 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2355.
    GeneTreeiENSGT00390000017920.
    HOGENOMiHOG000072016.
    HOVERGENiHBG002339.
    InParanoidiQ9DA79.
    KOiK01273.
    OMAiGGHSIDN.
    OrthoDBiEOG7SJD4N.
    PhylomeDBiQ9DA79.
    TreeFamiTF324523.

    Family and domain databases

    InterProiIPR000180. Dipep_AS.
    IPR028533. Dpep3.
    IPR008257. Renal_dipep_fam.
    [Graphical view]
    PANTHERiPTHR10443. PTHR10443. 1 hit.
    PTHR10443:SF14. PTHR10443:SF14. 1 hit.
    PfamiPF01244. Peptidase_M19. 1 hit.
    [Graphical view]
    PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
    PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9DA79-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MQPAGLEGPR ALGLRPLGHR LSLLGVLLLV PSLWVTCTLT TPSPSSAPTT
    60 70 80 90 100
    PEASNATTAP GIPNDTATSG VTSDPRLREQ ALALMRDFPL VDGHNDLPLL
    110 120 130 140 150
    LRELFQNQLQ DVNLRNFTRG QTNLDRLRDG LVGAQFWSAY IPCQTQDRDA
    160 170 180 190 200
    VRLALEQIDL IRRMCSAYPE LELVTSADGL NNTQKLACLI GVEGGHSLDT
    210 220 230 240 250
    SLAVLRSFYE LGVRYLTLTF TCSTPWAESA TKFRHHFYTN ISGLTSFGEK
    260 270 280 290 300
    VVEEMNRLGM MIDLSHASDT LVKQTLEVSQ APVIFSHSAA RSVCDNLLNI
    310 320 330 340 350
    PDDILQLLKK NGGIVMVTLS MGVLQCSLFA NVSTVADHFD HIRTVIGSEF
    360 370 380 390 400
    IGIGGSYDGS GRFPQGLEDV STYPVLIEEL LSRGWDEREL QGVLRGNLLR
    410 420 430 440 450
    VFRQVEQVRE KSLGQSPVEV KFPERQQSNT CHSHLLPQPQ EDQHQDTHLK
    460 470 480 490
    VTKLPNILQR ASKAPPHPLP GLMATLTSLA LILWLCCSGH RAV
    Length:493
    Mass (Da):54,247
    Last modified:June 1, 2001 - v1
    Checksum:iB9A0D39BB7CEB619
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF488553 mRNA. Translation: AAP84987.1.
    AK006085 mRNA. Translation: BAB24402.1.
    BC051148 mRNA. Translation: AAH51148.1.
    CCDSiCCDS22624.1.
    RefSeqiNP_082236.1. NM_027960.2.
    UniGeneiMm.173395.

    Genome annotation databases

    EnsembliENSMUST00000034371; ENSMUSP00000034371; ENSMUSG00000031898.
    GeneIDi71854.
    KEGGimmu:71854.
    UCSCiuc009net.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF488553 mRNA. Translation: AAP84987.1.
    AK006085 mRNA. Translation: BAB24402.1.
    BC051148 mRNA. Translation: AAH51148.1.
    CCDSiCCDS22624.1.
    RefSeqiNP_082236.1. NM_027960.2.
    UniGeneiMm.173395.

    3D structure databases

    ProteinModelPortaliQ9DA79.
    SMRiQ9DA79. Positions 77-434.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ9DA79. 1 interaction.
    MINTiMINT-8174108.
    STRINGi10090.ENSMUSP00000034371.

    Protein family/group databases

    MEROPSiM19.011.

    PTM databases

    PhosphoSiteiQ9DA79.

    Proteomic databases

    PaxDbiQ9DA79.
    PRIDEiQ9DA79.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000034371; ENSMUSP00000034371; ENSMUSG00000031898.
    GeneIDi71854.
    KEGGimmu:71854.
    UCSCiuc009net.1. mouse.

    Organism-specific databases

    CTDi64180.
    MGIiMGI:1919104. Dpep3.

    Phylogenomic databases

    eggNOGiCOG2355.
    GeneTreeiENSGT00390000017920.
    HOGENOMiHOG000072016.
    HOVERGENiHBG002339.
    InParanoidiQ9DA79.
    KOiK01273.
    OMAiGGHSIDN.
    OrthoDBiEOG7SJD4N.
    PhylomeDBiQ9DA79.
    TreeFamiTF324523.

    Miscellaneous databases

    NextBioi334738.
    PROiQ9DA79.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ9DA79.
    CleanExiMM_DPEP3.
    MM_MBD3.
    GenevisibleiQ9DA79. MM.

    Family and domain databases

    InterProiIPR000180. Dipep_AS.
    IPR028533. Dpep3.
    IPR008257. Renal_dipep_fam.
    [Graphical view]
    PANTHERiPTHR10443. PTHR10443. 1 hit.
    PTHR10443:SF14. PTHR10443:SF14. 1 hit.
    PfamiPF01244. Peptidase_M19. 1 hit.
    [Graphical view]
    PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
    PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Identification of two additional members of the membrane-bound dipeptidase family."
      Habib G.M., Shi Z.-Z., Cuevas A.A., Lieberman M.W.
      FASEB J. 17:1313-1315(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION.
      Strain: L129.
      Tissue: Spleen and Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    4. "Screening of genes involved in chromosome segregation during meiosis I: toward the identification of genes responsible for infertility in humans."
      Kogo H., Kowa-Sugiyama H., Yamada K., Bolor H., Tsutsumi M., Ohye T., Inagaki H., Taniguchi M., Toda T., Kurahashi H.
      J. Hum. Genet. 55:293-299(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, FUNCTION, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiDPEP3_MOUSE
    AccessioniPrimary (citable) accession number: Q9DA79
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: June 1, 2001
    Last modified: July 22, 2015
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.