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Protein

Sperm acrosome membrane-associated protein 1

Gene

Spaca1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in acrosome expansion and establishment of normal sperm morphology during spermatogenesis. Important for male fertility.1 Publication

GO - Molecular functioni

GO - Biological processi

  • acrosome assembly Source: MGI
  • cell wall macromolecule catabolic process Source: GO_Central
  • defense response to Gram-negative bacterium Source: GO_Central
  • defense response to Gram-positive bacterium Source: GO_Central
  • spermatogenesis Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm acrosome membrane-associated protein 1
Gene namesi
Name:Spaca1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914902 Spaca1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 231ExtracellularSequence analysisAdd BLAST203
Transmembranei232 – 252HelicalSequence analysisAdd BLAST21
Topological domaini253 – 305CytoplasmicSequence analysisAdd BLAST53

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

Males are sterile, while female fertility is not affected. Spermatozoa have significantly impaired swimming ability. The sperm head has a rounded shape instead of the normal hook-shaped morphology. The acrosome membrane does not associate normally with the nuclear envelope or intermediate filament bundles, and fails to expand along the nuclear surface. The sperm nucleus remains globular and mitochondria appear disorganized.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000024815329 – 305Sperm acrosome membrane-associated protein 1Add BLAST277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi30N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei266PhosphoserineBy similarity1
Modified residuei279PhosphotyrosineBy similarity1
Modified residuei288PhosphoserineBy similarity1
Modified residuei301PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9DA48
PRIDEiQ9DA48

PTM databases

iPTMnetiQ9DA48
PhosphoSitePlusiQ9DA48

Expressioni

Tissue specificityi

Testis specific (at protein level).1 Publication

Developmental stagei

Detected in testis from 3 weeks of age onwards.1 Publication

Gene expression databases

BgeeiENSMUSG00000028264
CleanExiMM_SPACA1
ExpressionAtlasiQ9DA48 baseline and differential
GenevisibleiQ9DA48 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029927

Structurei

3D structure databases

ProteinModelPortaliQ9DA48
SMRiQ9DA48
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 90Glu-richAdd BLAST45

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFV6 Eukaryota
ENOG410YPX9 LUCA
GeneTreeiENSGT00390000004211
HOGENOMiHOG000049221
HOVERGENiHBG080320
InParanoidiQ9DA48
OMAiWGAKAST
OrthoDBiEOG091G0M9J
PhylomeDBiQ9DA48
TreeFamiTF336918

Family and domain databases

CDDicd13783 SPACA1, 1 hit
InterProiView protein in InterPro
IPR037878 SPACA1

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DA48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRARGAGCPA GLLAVGWLIL VGLQASQASN VTSSGGGVQE PAVAREGESE
60 70 80 90 100
SESESEEEAE NEGEVPESET TAEADAEEEV QNRTIVKEVE FGMCTVTCGV
110 120 130 140 150
GIREVILTNG CPGGESKCVV RVEECRGPVD CGWGKPISEN LDSARLSCVH
160 170 180 190 200
ISPENRFKYV WKLLKPDQQP VILTNDSAVL EITREIRPLA FECDTLDNNE
210 220 230 240 250
MVASVKFTVY TTNELQMRRS SRPDTDAVLV FVLTIGVIIC IFVIFVLIFI
260 270 280 290 300
IINWAAVKSF WGSKTSATEI QSELSSMRYK DSTSLDQSPT DIPVHEDDAL

SEWNE
Length:305
Mass (Da):33,343
Last modified:June 1, 2001 - v1
Checksum:i9D68F7322E2DB398
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006187 mRNA Translation: BAB24447.1
CCDSiCCDS18026.1
RefSeqiNP_080569.1, NM_026293.3
UniGeneiMm.55848

Genome annotation databases

EnsembliENSMUST00000029927; ENSMUSP00000029927; ENSMUSG00000028264
GeneIDi67652
KEGGimmu:67652
UCSCiuc008sfz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSACA1_MOUSE
AccessioniPrimary (citable) accession number: Q9DA48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health