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Q9DA01

- EPPI_MOUSE

UniProt

Q9DA01 - EPPI_MOUSE

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Protein

Eppin

Gene

Eppin

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility (By similarity).By similarity

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

  1. defense response to bacterium Source: UniProtKB
  2. negative regulation of peptidase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI02.956.

Names & Taxonomyi

Protein namesi
Recommended name:
Eppin
Alternative name(s):
Epididymal protease inhibitor
Serine protease inhibitor-like with Kunitz and WAP domains 1
Gene namesi
Name:Eppin
Synonyms:Spinlw1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1922776. Eppin.

Subcellular locationi

Secreted. Cell surface
Note: Present on the surface of spermatozoa both before and after capacitation.

GO - Cellular componenti

  1. acrosomal vesicle Source: MGI
  2. cytoplasm Source: MGI
  3. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 134113EppinPRO_0000041380Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 61By similarity
Disulfide bondi40 ↔ 65By similarity
Disulfide bondi48 ↔ 60By similarity
Disulfide bondi54 ↔ 69By similarity
Disulfide bondi77 ↔ 127By similarity
Disulfide bondi86 ↔ 110By similarity
Disulfide bondi102 ↔ 123By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ9DA01.

Expressioni

Tissue specificityi

Expressed in differentiated spermatogonia in testis. Expressed in spermatogonia cell lines GC-1 spg and GC-2spd(ts) as well as in the Leydig tumor cell line MLTC-1 (at protein level). Expressed specifically in epididymis and testis. Expressed predominantly on the postacrosomal region of mouse spermatozoa, in Sertoli cells, Leydig cells, and round spermatids in the testis, and in the principal cells of the cauda epididymidis epithelium.2 Publications

Inductioni

Androgen dependent. Down-regulated in Sertoli cell-selective androgen receptor knockout mice.1 Publication

Gene expression databases

BgeeiQ9DA01.
GenevestigatoriQ9DA01.

Interactioni

Subunit structurei

Monomer. Homodimer. Homomultimers. Interacts (via C-terminus) with SEMG1 (via 164-283 AA). Interacts with LTF. Found in a complex with LTF, CLU, EPPIN and SEMG1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099389.

Structurei

3D structure databases

ProteinModelPortaliQ9DA01.
SMRiQ9DA01. Positions 38-129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 7348WAPPROSITE-ProRule annotationAdd
BLAST
Domaini77 – 12751BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni102 – 13332Interaction with SEMG1By similarityAdd
BLAST
Regioni117 – 13317Interaction with LTFBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 BPTI/Kunitz inhibitor domain.PROSITE-ProRule annotation
Contains 1 WAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG314382.
GeneTreeiENSGT00730000110932.
HOGENOMiHOG000115819.
HOVERGENiHBG004024.
InParanoidiQ9DA01.
OMAiFPRRCPK.
OrthoDBiEOG754HSR.
PhylomeDBiQ9DA01.
TreeFamiTF342459.

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
4.10.75.10. 1 hit.
InterProiIPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
IPR008197. WAP.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 1 hit.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMiSSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DA01-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLSGFVSIL VLFGLLARVQ GPSLADLLFP RRCPRFREEC EHQERDLCTR
60 70 80 90 100
DRDCPKKEKC CVFNCGKKCL NPQQDICSLP KDSGYCMAYF RRWWFNKENS
110 120 130
TCQVFIYGGC QGNNNNFQSQ SICQNACEKK SSLT
Length:134
Mass (Da):15,470
Last modified:May 4, 2001 - v1
Checksum:iDFFEB63D4D4C427F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF346413 mRNA. Translation: AAK31335.1.
AK006296 mRNA. Translation: BAB24514.1.
AL591478 Genomic DNA. Translation: CAM15019.1.
CH466551 Genomic DNA. Translation: EDL06403.1.
BC048637 mRNA. Translation: AAH48637.1.
CCDSiCCDS17043.1.
RefSeqiNP_083601.1. NM_029325.2.
UniGeneiMm.159533.

Genome annotation databases

EnsembliENSMUST00000103100; ENSMUSP00000099389; ENSMUSG00000017733.
GeneIDi75526.
KEGGimmu:75526.
UCSCiuc008nvh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF346413 mRNA. Translation: AAK31335.1 .
AK006296 mRNA. Translation: BAB24514.1 .
AL591478 Genomic DNA. Translation: CAM15019.1 .
CH466551 Genomic DNA. Translation: EDL06403.1 .
BC048637 mRNA. Translation: AAH48637.1 .
CCDSi CCDS17043.1.
RefSeqi NP_083601.1. NM_029325.2.
UniGenei Mm.159533.

3D structure databases

ProteinModelPortali Q9DA01.
SMRi Q9DA01. Positions 38-129.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000099389.

Protein family/group databases

MEROPSi I02.956.

Proteomic databases

PRIDEi Q9DA01.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000103100 ; ENSMUSP00000099389 ; ENSMUSG00000017733 .
GeneIDi 75526.
KEGGi mmu:75526.
UCSCi uc008nvh.1. mouse.

Organism-specific databases

CTDi 57119.
MGIi MGI:1922776. Eppin.

Phylogenomic databases

eggNOGi NOG314382.
GeneTreei ENSGT00730000110932.
HOGENOMi HOG000115819.
HOVERGENi HBG004024.
InParanoidi Q9DA01.
OMAi FPRRCPK.
OrthoDBi EOG754HSR.
PhylomeDBi Q9DA01.
TreeFami TF342459.

Miscellaneous databases

NextBioi 343260.
PROi Q9DA01.
SOURCEi Search...

Gene expression databases

Bgeei Q9DA01.
Genevestigatori Q9DA01.

Family and domain databases

Gene3Di 4.10.410.10. 1 hit.
4.10.75.10. 1 hit.
InterProi IPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
IPR008197. WAP.
[Graphical view ]
Pfami PF00014. Kunitz_BPTI. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view ]
PRINTSi PR00759. BASICPTASE.
SMARTi SM00131. KU. 1 hit.
SM00217. WAP. 1 hit.
[Graphical view ]
SUPFAMi SSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 1 hit.
PROSITEi PS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of mouse Eppin and a gene cluster of similar protease inhibitors on mouse chromosome 2."
    Sivashanmugam P., Hall S.H., Hamil K.G., French F.S., O'Rand M.G., Richardson R.T.
    Gene 312:125-134(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: BALB/c.
    Tissue: Epididymis and Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The effect of a sertoli cell-selective knockout of the androgen receptor on testicular gene expression in prepubertal mice."
    Denolet E., De Gendt K., Allemeersch J., Engelen K., Marchal K., Van Hummelen P., Tan K.A., Sharpe R.M., Saunders P.T., Swinnen J.V., Verhoeven G.
    Mol. Endocrinol. 20:321-334(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Distribution of Eppin in mouse and human testis."
    Long Y., Gu A., Yang H., Ji G., Han X., Song L., Wang S., Wang X.
    Mol. Med. Report. 4:71-75(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiEPPI_MOUSE
AccessioniPrimary (citable) accession number: Q9DA01
Secondary accession number(s): A2A5G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 4, 2001
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3