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Protein

Eppin

Gene

Eppin

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Protease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-MMU-6803157. Antimicrobial peptides.

Protein family/group databases

MEROPSiI17.953.

Names & Taxonomyi

Protein namesi
Recommended name:
Eppin
Alternative name(s):
Epididymal protease inhibitor
Serine protease inhibitor-like with Kunitz and WAP domains 1
Gene namesi
Name:Eppin
Synonyms:Spinlw1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1922776. Eppin.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: MGI
  • cell surface Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000004138022 – 134EppinAdd BLAST113

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 61By similarity
Disulfide bondi40 ↔ 65By similarity
Disulfide bondi48 ↔ 60By similarity
Disulfide bondi54 ↔ 69By similarity
Disulfide bondi77 ↔ 127By similarity
Disulfide bondi86 ↔ 110By similarity
Disulfide bondi102 ↔ 123By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9DA01.
PRIDEiQ9DA01.

Expressioni

Tissue specificityi

Expressed in differentiated spermatogonia in testis. Expressed in spermatogonia cell lines GC-1 spg and GC-2spd(ts) as well as in the Leydig tumor cell line MLTC-1 (at protein level). Expressed specifically in epididymis and testis. Expressed predominantly on the postacrosomal region of mouse spermatozoa, in Sertoli cells, Leydig cells, and round spermatids in the testis, and in the principal cells of the cauda epididymidis epithelium.2 Publications

Inductioni

Androgen dependent. Down-regulated in Sertoli cell-selective androgen receptor knockout mice.1 Publication

Gene expression databases

BgeeiENSMUSG00000017733.
GenevisibleiQ9DA01. MM.

Interactioni

Subunit structurei

Monomer. Homodimer. Homomultimers. Interacts (via C-terminus) with SEMG1 (via 164-283 AA). Interacts with LTF. Found in a complex with LTF, CLU, EPPIN and SEMG1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099389.

Structurei

3D structure databases

ProteinModelPortaliQ9DA01.
SMRiQ9DA01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 73WAPPROSITE-ProRule annotationAdd BLAST48
Domaini77 – 127BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST51

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 133Interaction with SEMG1By similarityAdd BLAST32
Regioni117 – 133Interaction with LTFBy similarityAdd BLAST17

Sequence similaritiesi

Contains 1 BPTI/Kunitz inhibitor domain.PROSITE-ProRule annotation
Contains 1 WAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00730000110932.
HOGENOMiHOG000115819.
HOVERGENiHBG004024.
InParanoidiQ9DA01.
OMAiANVQGPG.
OrthoDBiEOG091G09P2.
PhylomeDBiQ9DA01.
TreeFamiTF342459.

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
4.10.75.10. 1 hit.
InterProiIPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
IPR008197. WAP_dom.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 1 hit.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMiSSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DA01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSGFVSIL VLFGLLARVQ GPSLADLLFP RRCPRFREEC EHQERDLCTR
60 70 80 90 100
DRDCPKKEKC CVFNCGKKCL NPQQDICSLP KDSGYCMAYF RRWWFNKENS
110 120 130
TCQVFIYGGC QGNNNNFQSQ SICQNACEKK SSLT
Length:134
Mass (Da):15,470
Last modified:May 4, 2001 - v1
Checksum:iDFFEB63D4D4C427F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF346413 mRNA. Translation: AAK31335.1.
AK006296 mRNA. Translation: BAB24514.1.
AL591478 Genomic DNA. Translation: CAM15019.1.
CH466551 Genomic DNA. Translation: EDL06403.1.
BC048637 mRNA. Translation: AAH48637.1.
CCDSiCCDS17043.1.
RefSeqiNP_083601.1. NM_029325.2.
UniGeneiMm.159533.

Genome annotation databases

EnsembliENSMUST00000103100; ENSMUSP00000099389; ENSMUSG00000017733.
GeneIDi75526.
KEGGimmu:75526.
UCSCiuc008nvh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF346413 mRNA. Translation: AAK31335.1.
AK006296 mRNA. Translation: BAB24514.1.
AL591478 Genomic DNA. Translation: CAM15019.1.
CH466551 Genomic DNA. Translation: EDL06403.1.
BC048637 mRNA. Translation: AAH48637.1.
CCDSiCCDS17043.1.
RefSeqiNP_083601.1. NM_029325.2.
UniGeneiMm.159533.

3D structure databases

ProteinModelPortaliQ9DA01.
SMRiQ9DA01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099389.

Protein family/group databases

MEROPSiI17.953.

Proteomic databases

PaxDbiQ9DA01.
PRIDEiQ9DA01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103100; ENSMUSP00000099389; ENSMUSG00000017733.
GeneIDi75526.
KEGGimmu:75526.
UCSCiuc008nvh.1. mouse.

Organism-specific databases

CTDi57119.
MGIiMGI:1922776. Eppin.

Phylogenomic databases

eggNOGiKOG4295. Eukaryota.
ENOG410XQNP. LUCA.
GeneTreeiENSGT00730000110932.
HOGENOMiHOG000115819.
HOVERGENiHBG004024.
InParanoidiQ9DA01.
OMAiANVQGPG.
OrthoDBiEOG091G09P2.
PhylomeDBiQ9DA01.
TreeFamiTF342459.

Enzyme and pathway databases

ReactomeiR-MMU-6803157. Antimicrobial peptides.

Miscellaneous databases

PROiQ9DA01.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017733.
GenevisibleiQ9DA01. MM.

Family and domain databases

Gene3Di4.10.410.10. 1 hit.
4.10.75.10. 1 hit.
InterProiIPR002223. Kunitz_BPTI.
IPR020901. Prtase_inh_Kunz-CS.
IPR008197. WAP_dom.
[Graphical view]
PfamiPF00014. Kunitz_BPTI. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSiPR00759. BASICPTASE.
SMARTiSM00131. KU. 1 hit.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMiSSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 1 hit.
PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEPPI_MOUSE
AccessioniPrimary (citable) accession number: Q9DA01
Secondary accession number(s): A2A5G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 4, 2001
Last modified: November 30, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.