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Q9DA01 (EPPI_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eppin
Alternative name(s):
Epididymal protease inhibitor
Serine protease inhibitor-like with Kunitz and WAP domains 1
Gene names
Name:Eppin
Synonyms:Spinlw1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length134 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility By similarity.

Subunit structure

Monomer By similarity. Homodimer By similarity. Homomultimers By similarity. Interacts (via C-terminus) with SEMG1 (via 164-283 AA) By similarity. Interacts with LTF By similarity. Found in a complex with LTF, CLU, EPPIN and SEMG1 By similarity.

Subcellular location

Secreted. Cell surface. Note: Present on the surface of spermatozoa both before and after capacitation. Ref.1 Ref.7

Tissue specificity

Expressed in differentiated spermatogonia in testis. Expressed in spermatogonia cell lines GC-1 spg and GC-2spd(ts) as well as in the Leydig tumor cell line MLTC-1 (at protein level). Expressed specifically in epididymis and testis. Expressed predominantly on the postacrosomal region of mouse spermatozoa, in Sertoli cells, Leydig cells, and round spermatids in the testis, and in the principal cells of the cauda epididymidis epithelium. Ref.1 Ref.7

Induction

Androgen dependent. Down-regulated in Sertoli cell-selective androgen receptor knockout mice. Ref.6

Sequence similarities

Contains 1 BPTI/Kunitz inhibitor domain.

Contains 1 WAP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 134113Eppin
PRO_0000041380

Regions

Domain26 – 7348WAP
Domain77 – 12751BPTI/Kunitz inhibitor
Region102 – 13332Interaction with SEMG1 By similarity
Region117 – 13317Interaction with LTF By similarity

Amino acid modifications

Disulfide bond33 ↔ 61 By similarity
Disulfide bond40 ↔ 65 By similarity
Disulfide bond48 ↔ 60 By similarity
Disulfide bond54 ↔ 69 By similarity
Disulfide bond77 ↔ 127 By similarity
Disulfide bond86 ↔ 110 By similarity
Disulfide bond102 ↔ 123 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9DA01 [UniParc].

Last modified May 4, 2001. Version 1.
Checksum: DFFEB63D4D4C427F

FASTA13415,470
        10         20         30         40         50         60 
MKLSGFVSIL VLFGLLARVQ GPSLADLLFP RRCPRFREEC EHQERDLCTR DRDCPKKEKC 

        70         80         90        100        110        120 
CVFNCGKKCL NPQQDICSLP KDSGYCMAYF RRWWFNKENS TCQVFIYGGC QGNNNNFQSQ 

       130 
SICQNACEKK SSLT 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of mouse Eppin and a gene cluster of similar protease inhibitors on mouse chromosome 2."
Sivashanmugam P., Hall S.H., Hamil K.G., French F.S., O'Rand M.G., Richardson R.T.
Gene 312:125-134(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: BALB/c.
Tissue: Epididymis and Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The effect of a sertoli cell-selective knockout of the androgen receptor on testicular gene expression in prepubertal mice."
Denolet E., De Gendt K., Allemeersch J., Engelen K., Marchal K., Van Hummelen P., Tan K.A., Sharpe R.M., Saunders P.T., Swinnen J.V., Verhoeven G.
Mol. Endocrinol. 20:321-334(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[7]"Distribution of Eppin in mouse and human testis."
Long Y., Gu A., Yang H., Ji G., Han X., Song L., Wang S., Wang X.
Mol. Med. Report. 4:71-75(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF346413 mRNA. Translation: AAK31335.1.
AK006296 mRNA. Translation: BAB24514.1.
AL591478 Genomic DNA. Translation: CAM15019.1.
CH466551 Genomic DNA. Translation: EDL06403.1.
BC048637 mRNA. Translation: AAH48637.1.
CCDSCCDS17043.1.
RefSeqNP_083601.1. NM_029325.2.
UniGeneMm.159533.

3D structure databases

ProteinModelPortalQ9DA01.
SMRQ9DA01. Positions 38-129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000099389.

Protein family/group databases

MEROPSI02.956.

Proteomic databases

PRIDEQ9DA01.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103100; ENSMUSP00000099389; ENSMUSG00000017733.
GeneID75526.
KEGGmmu:75526.
UCSCuc008nvh.1. mouse.

Organism-specific databases

CTD57119.
MGIMGI:1922776. Eppin.

Phylogenomic databases

eggNOGNOG314382.
GeneTreeENSGT00730000110932.
HOGENOMHOG000115819.
HOVERGENHBG004024.
InParanoidQ9DA01.
OMAFPRRCPK.
OrthoDBEOG754HSR.
PhylomeDBQ9DA01.
TreeFamTF342459.

Gene expression databases

BgeeQ9DA01.
GenevestigatorQ9DA01.

Family and domain databases

Gene3D4.10.410.10. 1 hit.
4.10.75.10. 1 hit.
InterProIPR002223. Prot_inh_Kunz-m.
IPR020901. Prtase_inh_Kunz-CS.
IPR008197. WAP-type_4-diS_core.
[Graphical view]
PfamPF00014. Kunitz_BPTI. 1 hit.
PF00095. WAP. 1 hit.
[Graphical view]
PRINTSPR00759. BASICPTASE.
SMARTSM00131. KU. 1 hit.
SM00217. WAP. 1 hit.
[Graphical view]
SUPFAMSSF57256. SSF57256. 1 hit.
SSF57362. SSF57362. 1 hit.
PROSITEPS00280. BPTI_KUNITZ_1. 1 hit.
PS50279. BPTI_KUNITZ_2. 1 hit.
PS51390. WAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio343260.
PROQ9DA01.
SOURCESearch...

Entry information

Entry nameEPPI_MOUSE
AccessionPrimary (citable) accession number: Q9DA01
Secondary accession number(s): A2A5G9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: May 4, 2001
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot