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Q9DA01

- EPPI_MOUSE

UniProt

Q9DA01 - EPPI_MOUSE

Protein

Eppin

Gene

Eppin

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility By similarity.By similarity

    GO - Molecular functioni

    1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB
    2. negative regulation of peptidase activity Source: UniProtKB

    Keywords - Molecular functioni

    Antimicrobial, Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI02.956.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eppin
    Alternative name(s):
    Epididymal protease inhibitor
    Serine protease inhibitor-like with Kunitz and WAP domains 1
    Gene namesi
    Name:Eppin
    Synonyms:Spinlw1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1922776. Eppin.

    Subcellular locationi

    Secreted. Cell surface
    Note: Present on the surface of spermatozoa both before and after capacitation.

    GO - Cellular componenti

    1. acrosomal vesicle Source: MGI
    2. cell surface Source: UniProtKB-SubCell
    3. cytoplasm Source: MGI
    4. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 134113EppinPRO_0000041380Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 61By similarity
    Disulfide bondi40 ↔ 65By similarity
    Disulfide bondi48 ↔ 60By similarity
    Disulfide bondi54 ↔ 69By similarity
    Disulfide bondi77 ↔ 127By similarity
    Disulfide bondi86 ↔ 110By similarity
    Disulfide bondi102 ↔ 123By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiQ9DA01.

    Expressioni

    Tissue specificityi

    Expressed in differentiated spermatogonia in testis. Expressed in spermatogonia cell lines GC-1 spg and GC-2spd(ts) as well as in the Leydig tumor cell line MLTC-1 (at protein level). Expressed specifically in epididymis and testis. Expressed predominantly on the postacrosomal region of mouse spermatozoa, in Sertoli cells, Leydig cells, and round spermatids in the testis, and in the principal cells of the cauda epididymidis epithelium.2 Publications

    Inductioni

    Androgen dependent. Down-regulated in Sertoli cell-selective androgen receptor knockout mice.1 Publication

    Gene expression databases

    BgeeiQ9DA01.
    GenevestigatoriQ9DA01.

    Interactioni

    Subunit structurei

    Monomer. Homodimer. Homomultimers. Interacts (via C-terminus) with SEMG1 (via 164-283 AA). Interacts with LTF. Found in a complex with LTF, CLU, EPPIN and SEMG1.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000099389.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9DA01.
    SMRiQ9DA01. Positions 38-129.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 7348WAPPROSITE-ProRule annotationAdd
    BLAST
    Domaini77 – 12751BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni102 – 13332Interaction with SEMG1By similarityAdd
    BLAST
    Regioni117 – 13317Interaction with LTFBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BPTI/Kunitz inhibitor domain.PROSITE-ProRule annotation
    Contains 1 WAP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG314382.
    GeneTreeiENSGT00730000110932.
    HOGENOMiHOG000115819.
    HOVERGENiHBG004024.
    InParanoidiQ9DA01.
    OMAiFPRRCPK.
    OrthoDBiEOG754HSR.
    PhylomeDBiQ9DA01.
    TreeFamiTF342459.

    Family and domain databases

    Gene3Di4.10.410.10. 1 hit.
    4.10.75.10. 1 hit.
    InterProiIPR002223. Prot_inh_Kunz-m.
    IPR020901. Prtase_inh_Kunz-CS.
    IPR008197. WAP.
    [Graphical view]
    PfamiPF00014. Kunitz_BPTI. 1 hit.
    PF00095. WAP. 1 hit.
    [Graphical view]
    PRINTSiPR00759. BASICPTASE.
    SMARTiSM00131. KU. 1 hit.
    SM00217. WAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF57256. SSF57256. 1 hit.
    SSF57362. SSF57362. 1 hit.
    PROSITEiPS00280. BPTI_KUNITZ_1. 1 hit.
    PS50279. BPTI_KUNITZ_2. 1 hit.
    PS51390. WAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9DA01-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLSGFVSIL VLFGLLARVQ GPSLADLLFP RRCPRFREEC EHQERDLCTR    50
    DRDCPKKEKC CVFNCGKKCL NPQQDICSLP KDSGYCMAYF RRWWFNKENS 100
    TCQVFIYGGC QGNNNNFQSQ SICQNACEKK SSLT 134
    Length:134
    Mass (Da):15,470
    Last modified:May 4, 2001 - v1
    Checksum:iDFFEB63D4D4C427F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF346413 mRNA. Translation: AAK31335.1.
    AK006296 mRNA. Translation: BAB24514.1.
    AL591478 Genomic DNA. Translation: CAM15019.1.
    CH466551 Genomic DNA. Translation: EDL06403.1.
    BC048637 mRNA. Translation: AAH48637.1.
    CCDSiCCDS17043.1.
    RefSeqiNP_083601.1. NM_029325.2.
    UniGeneiMm.159533.

    Genome annotation databases

    EnsembliENSMUST00000103100; ENSMUSP00000099389; ENSMUSG00000017733.
    GeneIDi75526.
    KEGGimmu:75526.
    UCSCiuc008nvh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF346413 mRNA. Translation: AAK31335.1 .
    AK006296 mRNA. Translation: BAB24514.1 .
    AL591478 Genomic DNA. Translation: CAM15019.1 .
    CH466551 Genomic DNA. Translation: EDL06403.1 .
    BC048637 mRNA. Translation: AAH48637.1 .
    CCDSi CCDS17043.1.
    RefSeqi NP_083601.1. NM_029325.2.
    UniGenei Mm.159533.

    3D structure databases

    ProteinModelPortali Q9DA01.
    SMRi Q9DA01. Positions 38-129.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000099389.

    Protein family/group databases

    MEROPSi I02.956.

    Proteomic databases

    PRIDEi Q9DA01.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103100 ; ENSMUSP00000099389 ; ENSMUSG00000017733 .
    GeneIDi 75526.
    KEGGi mmu:75526.
    UCSCi uc008nvh.1. mouse.

    Organism-specific databases

    CTDi 57119.
    MGIi MGI:1922776. Eppin.

    Phylogenomic databases

    eggNOGi NOG314382.
    GeneTreei ENSGT00730000110932.
    HOGENOMi HOG000115819.
    HOVERGENi HBG004024.
    InParanoidi Q9DA01.
    OMAi FPRRCPK.
    OrthoDBi EOG754HSR.
    PhylomeDBi Q9DA01.
    TreeFami TF342459.

    Miscellaneous databases

    NextBioi 343260.
    PROi Q9DA01.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9DA01.
    Genevestigatori Q9DA01.

    Family and domain databases

    Gene3Di 4.10.410.10. 1 hit.
    4.10.75.10. 1 hit.
    InterProi IPR002223. Prot_inh_Kunz-m.
    IPR020901. Prtase_inh_Kunz-CS.
    IPR008197. WAP.
    [Graphical view ]
    Pfami PF00014. Kunitz_BPTI. 1 hit.
    PF00095. WAP. 1 hit.
    [Graphical view ]
    PRINTSi PR00759. BASICPTASE.
    SMARTi SM00131. KU. 1 hit.
    SM00217. WAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57256. SSF57256. 1 hit.
    SSF57362. SSF57362. 1 hit.
    PROSITEi PS00280. BPTI_KUNITZ_1. 1 hit.
    PS50279. BPTI_KUNITZ_2. 1 hit.
    PS51390. WAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of mouse Eppin and a gene cluster of similar protease inhibitors on mouse chromosome 2."
      Sivashanmugam P., Hall S.H., Hamil K.G., French F.S., O'Rand M.G., Richardson R.T.
      Gene 312:125-134(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: BALB/c.
      Tissue: Epididymis and Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The effect of a sertoli cell-selective knockout of the androgen receptor on testicular gene expression in prepubertal mice."
      Denolet E., De Gendt K., Allemeersch J., Engelen K., Marchal K., Van Hummelen P., Tan K.A., Sharpe R.M., Saunders P.T., Swinnen J.V., Verhoeven G.
      Mol. Endocrinol. 20:321-334(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Distribution of Eppin in mouse and human testis."
      Long Y., Gu A., Yang H., Ji G., Han X., Song L., Wang S., Wang X.
      Mol. Med. Report. 4:71-75(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiEPPI_MOUSE
    AccessioniPrimary (citable) accession number: Q9DA01
    Secondary accession number(s): A2A5G9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: May 4, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3