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Protein

Sperm acrosome membrane-associated protein 3

Gene

Spaca3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro (By similarity).By similarity1 Publication

Caution

Although it belongs to the glycosyl hydrolase 22 family, Thr-128 and Asn-145 are present instead of the conserved Glu and Asp which are active site residues. It is therefore expected that this protein lacks hydrolase activity.Curated

GO - Molecular functioni

GO - Biological processi

  • cell wall macromolecule catabolic process Source: GO_Central
  • defense response to Gram-negative bacterium Source: GO_Central
  • defense response to Gram-positive bacterium Source: GO_Central
  • fusion of sperm to egg plasma membrane involved in single fertilization Source: UniProtKB
  • sperm-egg recognition Source: MGI

Protein family/group databases

CAZyiGH22 Glycoside Hydrolase Family 22

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm acrosome membrane-associated protein 3
Alternative name(s):
Lysozyme-like protein 3
Sperm lysozyme-like protein 1
Short name:
mSLLP1
Cleaved into the following 2 chains:
Gene namesi
Name:Spaca3
Synonyms:Lyc3, Lyzl3, Sllp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1922872 Spaca3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 69CytoplasmicSequence analysisAdd BLAST69
Transmembranei70 – 90Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini91 – 221ExtracellularSequence analysisAdd BLAST131

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002562211 – 221Sperm acrosome membrane-associated protein 3, membrane formAdd BLAST221
ChainiPRO_000025622295 – 221Sperm acrosome membrane-associated protein 3, processed formAdd BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi99 ↔ 219PROSITE-ProRule annotation1 Publication
Disulfide bondi123 ↔ 207PROSITE-ProRule annotation1 Publication
Disulfide bondi157 ↔ 172PROSITE-ProRule annotation1 Publication
Disulfide bondi168 ↔ 186PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei93 – 94Cleavage; to produce processed formBy similarity2

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9D9X8
PeptideAtlasiQ9D9X8
PRIDEiQ9D9X8

PTM databases

PhosphoSitePlusiQ9D9X8

Expressioni

Tissue specificityi

The processed form is expressed in sperm (at protein level). Expressed strongly in testis and epididymis and weakly in pancreas.2 Publications

Gene expression databases

BgeeiENSMUSG00000053184
CleanExiMM_SPACA3
ExpressionAtlasiQ9D9X8 baseline and differential
GenevisibleiQ9D9X8 MM

Interactioni

Subunit structurei

Interacts with ASTL.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099511

Structurei

Secondary structure

1221
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi98 – 107Combined sources10
Helixi113 – 115Combined sources3
Helixi118 – 129Combined sources12
Beta strandi136 – 138Combined sources3
Beta strandi144 – 146Combined sources3
Turni147 – 150Combined sources4
Turni153 – 155Combined sources3
Beta strandi156 – 158Combined sources3
Beta strandi160 – 162Combined sources3
Helixi172 – 176Combined sources5
Helixi181 – 191Combined sources11
Helixi196 – 199Combined sources4
Helixi201 – 206Combined sources6
Turni207 – 209Combined sources3
Helixi213 – 215Combined sources3
Turni216 – 218Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YF2X-ray2.15A/B/C93-221[»]
ProteinModelPortaliQ9D9X8
SMRiQ9D9X8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9D9X8

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 22 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG3E Eukaryota
ENOG41118PT LUCA
GeneTreeiENSGT00550000074398
HOGENOMiHOG000037357
HOVERGENiHBG052297
InParanoidiQ9D9X8
OMAiKDTVICA
PhylomeDBiQ9D9X8
TreeFamiTF324882

Family and domain databases

CDDicd00119 LYZ1, 1 hit
InterProiView protein in InterPro
IPR001916 Glyco_hydro_22
IPR019799 Glyco_hydro_22_CS
IPR000974 Glyco_hydro_22_lys
IPR023346 Lysozyme-like_dom_sf
IPR030058 LYZL3
PANTHERiPTHR11407:SF25 PTHR11407:SF25, 1 hit
PfamiView protein in Pfam
PF00062 Lys, 1 hit
PRINTSiPR00137 LYSOZYME
PR00135 LYZLACT
SMARTiView protein in SMART
SM00263 LYZ1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
PROSITEiView protein in PROSITE
PS00128 LACTALBUMIN_LYSOZYME_1, 1 hit
PS51348 LACTALBUMIN_LYSOZYME_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D9X8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGICMSMYTQ VLVPVDADGD HHILWSRFYE RWGSCFNPCA GLVNCLPPHS
60 70 80 90 100
SALYLCHRME ARSRAPRRQL CPPGITWLAL AYLLSCLLAS SKAKVFSRCE
110 120 130 140 150
LAKEMHDFGL DGYRGYNLAD WVCLAYYTSG FNTNAVDHEA DGSTNNGIFQ
160 170 180 190 200
ISSRRWCRTL ASNGPNLCRI YCTDLLNNDL KDSIVCAMKI VQEPLGLGYW
210 220
EAWRHHCQGR DLSDWVDGCD F
Note: No experimental confirmation available.
Length:221
Mass (Da):25,020
Last modified:October 31, 2006 - v2
Checksum:iE122BB82C730AA3B
GO
Isoform 2 (identifier: Q9D9X8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.

Show »
Length:163
Mass (Da):18,438
Checksum:i9FC152947A1B1A27
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61A → P in BAB24544 (PubMed:16141072).Curated1
Sequence conflicti66 – 67PR → SI in BAB24544 (PubMed:16141072).Curated2
Sequence conflicti212L → P in AAS77887 (PubMed:16014814).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0213301 – 58Missing in isoform 2. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY572448 mRNA Translation: AAS77887.1
AK006357 mRNA Translation: BAB24544.1
AL645842 Genomic DNA No translation available.
BC100503 mRNA Translation: AAI00504.1
AY601763 mRNA Translation: AAT07446.1
CCDSiCCDS25136.1 [Q9D9X8-2]
RefSeqiNP_083643.1, NM_029367.1 [Q9D9X8-2]
XP_011247596.1, XM_011249294.2 [Q9D9X8-2]
UniGeneiMm.26988

Genome annotation databases

EnsembliENSMUST00000103222; ENSMUSP00000099511; ENSMUSG00000053184 [Q9D9X8-2]
ENSMUST00000103223; ENSMUSP00000099512; ENSMUSG00000053184 [Q9D9X8-2]
GeneIDi75622
KEGGimmu:75622
UCSCiuc007kmk.1 mouse [Q9D9X8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSACA3_MOUSE
AccessioniPrimary (citable) accession number: Q9D9X8
Secondary accession number(s): Q5SUU3, Q6PKP1, Q6PX67
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: May 23, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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