Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase RNF125

Gene

Rnf125

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as DDX58/RIG-I, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53. Acts as a negative regulator of type I interferon production by mediating ubiquitination of DDX58/RIG-I at 'Lys-181', leading to DDX58/RIG-I degradation. Mediates ubiquitination and subsequent degradation of p53/TP53. Mediates ubiquitination and subsequent degradation of JAK1. Acts as a positive regulator of T-cell activation.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37Zinc 1By similarity1
Metal bindingi40Zinc 1By similarity1
Metal bindingi52Zinc 2By similarity1
Metal bindingi54Zinc 2By similarity1
Metal bindingi57Zinc 1By similarity1
Metal bindingi60Zinc 1By similarity1
Metal bindingi73Zinc 2By similarity1
Metal bindingi76Zinc 2By similarity1
Metal bindingi101Zinc 3By similarity1
Metal bindingi104Zinc 3By similarity1
Metal bindingi116Zinc 3By similarity1
Metal bindingi120Zinc 3By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri37 – 77RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri101 – 120C2HC RNF-typePROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAdaptive immunity, Immunity, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF125 (EC:2.3.2.27By similarity)
Alternative name(s):
RING finger protein 125
Gene namesi
Name:Rnf125
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1914914. Rnf125.

Subcellular locationi

  • Golgi apparatus membrane By similarity; Lipid-anchor By similarity

  • Note: Shows a reticular staining pattern within the cell and is probably expressed at other intracellular membranes in addition to the Golgi membrane. Not detected at the plasma membrane.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000560922 – 233E3 ubiquitin-protein ligase RNF125Add BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1

Post-translational modificationi

Autoubiquitinated, leading to its subsequent proteasomal degradation.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

PaxDbiQ9D9R0.
PRIDEiQ9D9R0.

PTM databases

iPTMnetiQ9D9R0.
PhosphoSitePlusiQ9D9R0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033107.
CleanExiMM_RNF125.
GenevisibleiQ9D9R0. MM.

Interactioni

Subunit structurei

Interacts with UBE2D1. Interacts with VCP/p97; leading to recruit RNF125 to DDX58/RIG-I and promote ubiquitination of DDX58/RIG-I.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058251.

Structurei

3D structure databases

ProteinModelPortaliQ9D9R0.
SMRiQ9D9R0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 45Interaction with the C2HC RNF-type zinc fingerBy similarity3
Regioni110 – 114Interaction with the RING-type zinc fingerBy similarity5
Regioni121 – 129Linker regionBy similarity9
Regioni211 – 225Required for interaction with ubiquitin and for autoubiquitinationAdd BLAST15

Domaini

The C2HC RNF-type zinc finger and the linker region stabilize the RING-type zinc finger, leading to promote binding of the RING-type zinc finger to the ubiquitin-conjugating enzyme E2 (donor ubiquitin).By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri37 – 77RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri101 – 120C2HC RNF-typePROSITE-ProRule annotationAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IJHI. Eukaryota.
ENOG410YBNH. LUCA.
GeneTreeiENSGT00530000063064.
HOGENOMiHOG000230946.
HOVERGENiHBG098958.
InParanoidiQ9D9R0.
KOiK12170.
OMAiVPNENAN.
OrthoDBiEOG091G0LBM.
PhylomeDBiQ9D9R0.
TreeFamiTF331012.

Family and domain databases

CDDicd00162. RING. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR008598. Di19_Zn_binding_dom.
IPR034734. ZF_C2HC_RNF.
IPR027370. Znf-RING_LisH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF05605. zf-Di19. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS51803. ZF_C2HC_RNF. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D9R0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSLLSSDSS KSAPASATPR TLERSGDSEL PITSFDCSVC LEVLHQPVRT
60 70 80 90 100
RCGHVFCRSC IATSIKNNNK WTCPYCRAYL PSEGVPATDI AKRMKSEYQN
110 120 130 140 150
CAECGTLVCL SDMRAHIRTC EKYIDKYGPL LELGDTTARC VCPFCQRELD
160 170 180 190 200
EDCLLDHCII HHRSERRPVF CPLCHSRPDE SPSTFNGSLI RHLQVSHTLF
210 220 230
YDDFIDFDII EEAIIRRVLD RSLLEYVNQS NTT
Length:233
Mass (Da):26,362
Last modified:January 23, 2007 - v3
Checksum:i1B92E1F4F6C0113A
GO
Isoform 2 (identifier: Q9D9R0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: Missing.

Show »
Length:140
Mass (Da):16,302
Checksum:i41DE755191A98B93
GO

Sequence cautioni

The sequence BAB24656 differs from that shown. Reason: Frameshift at position 72.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84G → R in BAC34234 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0152161 – 93Missing in isoform 2. 2 PublicationsAdd BLAST93

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006569 mRNA. Translation: BAB24656.1. Frameshift.
AK050395 mRNA. Translation: BAC34234.1.
AC138611 Genomic DNA. No translation available.
BC094290 mRNA. Translation: AAH94290.1.
BC116883 mRNA. Translation: AAI16884.1.
BC116887 mRNA. Translation: AAI16888.1.
CCDSiCCDS29088.1. [Q9D9R0-2]
RefSeqiNP_080577.1. NM_026301.2. [Q9D9R0-2]
XP_006526251.1. XM_006526188.3. [Q9D9R0-1]
UniGeneiMm.45980.

Genome annotation databases

EnsembliENSMUST00000050004; ENSMUSP00000058251; ENSMUSG00000033107. [Q9D9R0-2]
GeneIDi67664.
KEGGimmu:67664.
UCSCiuc008eez.1. mouse. [Q9D9R0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006569 mRNA. Translation: BAB24656.1. Frameshift.
AK050395 mRNA. Translation: BAC34234.1.
AC138611 Genomic DNA. No translation available.
BC094290 mRNA. Translation: AAH94290.1.
BC116883 mRNA. Translation: AAI16884.1.
BC116887 mRNA. Translation: AAI16888.1.
CCDSiCCDS29088.1. [Q9D9R0-2]
RefSeqiNP_080577.1. NM_026301.2. [Q9D9R0-2]
XP_006526251.1. XM_006526188.3. [Q9D9R0-1]
UniGeneiMm.45980.

3D structure databases

ProteinModelPortaliQ9D9R0.
SMRiQ9D9R0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058251.

PTM databases

iPTMnetiQ9D9R0.
PhosphoSitePlusiQ9D9R0.

Proteomic databases

PaxDbiQ9D9R0.
PRIDEiQ9D9R0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050004; ENSMUSP00000058251; ENSMUSG00000033107. [Q9D9R0-2]
GeneIDi67664.
KEGGimmu:67664.
UCSCiuc008eez.1. mouse. [Q9D9R0-1]

Organism-specific databases

CTDi54941.
MGIiMGI:1914914. Rnf125.

Phylogenomic databases

eggNOGiENOG410IJHI. Eukaryota.
ENOG410YBNH. LUCA.
GeneTreeiENSGT00530000063064.
HOGENOMiHOG000230946.
HOVERGENiHBG098958.
InParanoidiQ9D9R0.
KOiK12170.
OMAiVPNENAN.
OrthoDBiEOG091G0LBM.
PhylomeDBiQ9D9R0.
TreeFamiTF331012.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiRnf125. mouse.
PROiPR:Q9D9R0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033107.
CleanExiMM_RNF125.
GenevisibleiQ9D9R0. MM.

Family and domain databases

CDDicd00162. RING. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR008598. Di19_Zn_binding_dom.
IPR034734. ZF_C2HC_RNF.
IPR027370. Znf-RING_LisH.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF05605. zf-Di19. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS51803. ZF_C2HC_RNF. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRN125_MOUSE
AccessioniPrimary (citable) accession number: Q9D9R0
Secondary accession number(s): Q0VG32, Q52KL4, Q8C7F2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: May 10, 2017
This is version 126 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.