Q9D9Q6 (CALR3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calreticulin-3

Alternative name(s):
Calreticulin-2
Calsperin

Gene names

Name:	Calr3
Synonyms:	Crt2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	CALR3 capacity for calcium-binding may be absent or much lower than that of CALR By similarity. During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility. Ref.6
Subunit structure	Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Ref.5
Subcellular location	Endoplasmic reticulum lumen By similarity.
Tissue specificity	Testis specific, absent in mature sperm. Ref.4 Ref.6
Disruption phenotype	Defective sperm migration from the uterus into the oviduct and defective binding to the zona pellucida. Ref.6
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Molecular function	Chaperone
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from electronic annotation. Source: InterPro spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 380	361	Calreticulin-3		PRO_0000004179
Regions
	Repeat	191 – 202	12	1-1
	Repeat	209 – 220	12	1-2
	Repeat	222 – 231	10	1-3
	Repeat	235 – 246	12	1-4
	Repeat	250 – 256	7	2-1
	Repeat	260 – 268	9	2-2
	Repeat	270 – 280	11	2-3
	Region	20 – 197	178	N-domain
	Region	191 – 246	56	4 X approximate repeats
	Region	198 – 291	94	P-domain
	Region	250 – 280	31	3 X approximate repeats
	Region	292 – 380	89	C-domain
	Motif	377 – 380	4	Prevents secretion from ER Potential
Sites
	Binding site	109	1	Carbohydrate By similarity
	Binding site	111	1	Carbohydrate By similarity
	Binding site	128	1	Carbohydrate By similarity
	Binding site	135	1	Carbohydrate By similarity
	Binding site	300	1	Carbohydrate By similarity
Amino acid modifications
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential
	Disulfide bond	137 ↔ 163		By similarity
Experimental info
	Sequence conflict	371	1	F → L in BAB24660. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9D9Q6 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: B13366ADB11B0442	FASTA	380	44,232
	10 20 30 40 50 60 MVSARALLWA ICVLRVALAT VYFQEEFLDG ERWRNRWVQS TNDSQFGHFR VSSGKFYGHK 70 80 90 100 110 120 EKDKGLQTTQ NSRFYAISAS FKPFSNKGKT LVIQYTVKHE QKMDCGGGYI KVFPSDLDQK 130 140 150 160 170 180 KMNGKSQYYI MFGPDICGFD IKKVHVILYF KNQYHENKKP IRCKVDGFTH LYTLILRPDL 190 200 210 220 230 240 SYEVKVDGQS IESGSIEYDW NLTSLRKTEK TSLDSRDWDQ VEGSKVQDWE KHFLDAGASK 250 260 270 280 290 300 PSDWNSELDG DWLQKPPYED GLKAEGIDKD VWLHQKMRPA GYLTQYDLSE FENIGAIGLE 310 320 330 340 350 360 LWQVRSGTIF DNFLITDDEE YAEKFGKATW GETKGPEKEM DAIQAKEEVK KAREEDEEDL 370 380 LMGKFHRHNH FSRFHRQGEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Testis.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Identification of a novel calreticulin isoform (Crt2) in human and mouse." Persson S., Rosenquist M., Sommarin M. Gene 297:151-158(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[5]	"Age-specific CUGBP1-eIF2 complex increases translation of CCAAT/enhancer-binding protein beta in old liver." Timchenko L.T., Salisbury E., Wang G.-L., Nguyen H., Albrecht J.H., Hershey J.W., Timchenko N.A. J. Biol. Chem. 281:32806-32819(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN AN EIF2 COMPLEX WITH EIF2S1; EIF2S2; CALR; CELF1; HSPA5 AND HSP90B1.
[6]	"Calsperin is a testis-specific chaperone required for sperm fertility." Ikawa M., Tokuhiro K., Yamaguchi R., Benham A.M., Tamura T., Wada I., Satouh Y., Inoue N., Okabe M. J. Biol. Chem. 286:5639-5646(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK006582 mRNA. Translation: BAB24660.1. AC113182 Genomic DNA. No translation available. CH466525 Genomic DNA. Translation: EDL10797.1.
IPI	IPI00113023.
RefSeq	NP_082776.2. NM_028500.3.
UniGene	Mm.196315.
3D structure databases
ProteinModelPortal	Q9D9Q6.
SMR	Q9D9Q6. Positions 21-348.
ModBase	Search...
PTM databases
PhosphoSite	Q9D9Q6.
Proteomic databases
PaxDb	Q9D9Q6.
PRIDE	Q9D9Q6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000019876; ENSMUSP00000019876; ENSMUSG00000019732.
GeneID	73316.
KEGG	mmu:73316.
Organism-specific databases
CTD	125972.
MGI	MGI:1920566. Calr3.
Phylogenomic databases
eggNOG	NOG268919.
GeneTree	ENSGT00430000030841.
HOGENOM	HOG000192435.
HOVERGEN	HBG005407.
InParanoid	Q9D9Q6.
KO	K10098.
OMA	QDWEKHF.
OrthoDB	EOG41JZCD.
Gene expression databases
CleanEx	MM_CALR3.
Genevestigator	Q9D9Q6.
GermOnline	ENSMUSG00000019732. Mus musculus.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009169. Calreticulin. IPR009033. Calreticulin/calnexin_P_dom. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PIRSF	PIRSF002356. Calreticulin. 1 hit.
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF63887. Calret_calnex_P. 1 hit. SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00014. ER_TARGET. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	337937.
SOURCE	Search...

Entry information

Entry name

CALR3_MOUSE

Accession

Primary (citable) accession number: Q9D9Q6
Secondary accession number(s): G5E827

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2003
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program