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Q9D9Q6

- CALR3_MOUSE

UniProt

Q9D9Q6 - CALR3_MOUSE

Protein

Calreticulin-3

Gene

Calr3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    CALR3 capacity for calcium-binding may be absent or much lower than that of CALR By similarity. During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility.By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091CarbohydrateBy similarity
    Binding sitei111 – 1111CarbohydrateBy similarity
    Binding sitei128 – 1281CarbohydrateBy similarity
    Binding sitei135 – 1351CarbohydrateBy similarity
    Binding sitei300 – 3001CarbohydrateBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. protein binding Source: MGI
    3. protein binding involved in protein folding Source: MGI

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. spermatogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calreticulin-3
    Alternative name(s):
    Calreticulin-2
    Calsperin
    Gene namesi
    Name:Calr3
    Synonyms:Crt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1920566. Calr3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Disruption phenotypei

    Defective sperm migration from the uterus into the oviduct and defective binding to the zona pellucida.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 380361Calreticulin-3PRO_0000004179Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi42 – 421N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi137 ↔ 163By similarity
    Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9D9Q6.
    PRIDEiQ9D9Q6.

    PTM databases

    PhosphoSiteiQ9D9Q6.

    Expressioni

    Tissue specificityi

    Testis specific, absent in mature sperm.2 Publications

    Gene expression databases

    CleanExiMM_CALR3.
    GenevestigatoriQ9D9Q6.

    Interactioni

    Subunit structurei

    Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.1 Publication

    Protein-protein interaction databases

    DIPiDIP-60025N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D9Q6.
    SMRiQ9D9Q6. Positions 21-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati191 – 202121-1Add
    BLAST
    Repeati209 – 220121-2Add
    BLAST
    Repeati222 – 231101-3
    Repeati235 – 246121-4Add
    BLAST
    Repeati250 – 25672-1
    Repeati260 – 26892-2
    Repeati270 – 280112-3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni20 – 197178N-domainAdd
    BLAST
    Regioni191 – 246564 X approximate repeatsAdd
    BLAST
    Regioni198 – 29194P-domainAdd
    BLAST
    Regioni250 – 280313 X approximate repeatsAdd
    BLAST
    Regioni292 – 38089C-domainAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi377 – 3804Prevents secretion from ERSequence Analysis

    Sequence similaritiesi

    Belongs to the calreticulin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG268919.
    GeneTreeiENSGT00430000030841.
    HOGENOMiHOG000192435.
    HOVERGENiHBG005407.
    InParanoidiQ9D9Q6.
    KOiK10098.
    OMAiQDWEKHF.
    OrthoDBiEOG77126Z.
    TreeFamiTF338438.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view]
    PANTHERiPTHR11073. PTHR11073. 1 hit.
    PfamiPF00262. Calreticulin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002356. Calreticulin. 1 hit.
    PRINTSiPR00626. CALRETICULIN.
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF63887. SSF63887. 1 hit.
    PROSITEiPS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9D9Q6-1 [UniParc]FASTAAdd to Basket

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    MVSARALLWA ICVLRVALAT VYFQEEFLDG ERWRNRWVQS TNDSQFGHFR    50
    VSSGKFYGHK EKDKGLQTTQ NSRFYAISAS FKPFSNKGKT LVIQYTVKHE 100
    QKMDCGGGYI KVFPSDLDQK KMNGKSQYYI MFGPDICGFD IKKVHVILYF 150
    KNQYHENKKP IRCKVDGFTH LYTLILRPDL SYEVKVDGQS IESGSIEYDW 200
    NLTSLRKTEK TSLDSRDWDQ VEGSKVQDWE KHFLDAGASK PSDWNSELDG 250
    DWLQKPPYED GLKAEGIDKD VWLHQKMRPA GYLTQYDLSE FENIGAIGLE 300
    LWQVRSGTIF DNFLITDDEE YAEKFGKATW GETKGPEKEM DAIQAKEEVK 350
    KAREEDEEDL LMGKFHRHNH FSRFHRQGEL 380
    Length:380
    Mass (Da):44,232
    Last modified:October 3, 2012 - v2
    Checksum:iB13366ADB11B0442
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti371 – 3711F → L in BAB24660. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK006582 mRNA. Translation: BAB24660.1.
    AC113182 Genomic DNA. No translation available.
    CH466525 Genomic DNA. Translation: EDL10797.1.
    CCDSiCCDS22413.1.
    RefSeqiNP_082776.2. NM_028500.3.
    UniGeneiMm.196315.

    Genome annotation databases

    EnsembliENSMUST00000019876; ENSMUSP00000019876; ENSMUSG00000019732.
    GeneIDi73316.
    KEGGimmu:73316.
    UCSCiuc009mfx.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK006582 mRNA. Translation: BAB24660.1 .
    AC113182 Genomic DNA. No translation available.
    CH466525 Genomic DNA. Translation: EDL10797.1 .
    CCDSi CCDS22413.1.
    RefSeqi NP_082776.2. NM_028500.3.
    UniGenei Mm.196315.

    3D structure databases

    ProteinModelPortali Q9D9Q6.
    SMRi Q9D9Q6. Positions 21-348.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60025N.

    PTM databases

    PhosphoSitei Q9D9Q6.

    Proteomic databases

    PaxDbi Q9D9Q6.
    PRIDEi Q9D9Q6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019876 ; ENSMUSP00000019876 ; ENSMUSG00000019732 .
    GeneIDi 73316.
    KEGGi mmu:73316.
    UCSCi uc009mfx.1. mouse.

    Organism-specific databases

    CTDi 125972.
    MGIi MGI:1920566. Calr3.

    Phylogenomic databases

    eggNOGi NOG268919.
    GeneTreei ENSGT00430000030841.
    HOGENOMi HOG000192435.
    HOVERGENi HBG005407.
    InParanoidi Q9D9Q6.
    KOi K10098.
    OMAi QDWEKHF.
    OrthoDBi EOG77126Z.
    TreeFami TF338438.

    Miscellaneous databases

    NextBioi 337937.
    PROi Q9D9Q6.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_CALR3.
    Genevestigatori Q9D9Q6.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR001580. Calret/calnex.
    IPR018124. Calret/calnex_CS.
    IPR009169. Calreticulin.
    IPR009033. Calreticulin/calnexin_P_dom.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    [Graphical view ]
    PANTHERi PTHR11073. PTHR11073. 1 hit.
    Pfami PF00262. Calreticulin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002356. Calreticulin. 1 hit.
    PRINTSi PR00626. CALRETICULIN.
    SUPFAMi SSF49899. SSF49899. 2 hits.
    SSF63887. SSF63887. 1 hit.
    PROSITEi PS00803. CALRETICULIN_1. 1 hit.
    PS00804. CALRETICULIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Identification of a novel calreticulin isoform (Crt2) in human and mouse."
      Persson S., Rosenquist M., Sommarin M.
      Gene 297:151-158(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
    5. "Age-specific CUGBP1-eIF2 complex increases translation of CCAAT/enhancer-binding protein beta in old liver."
      Timchenko L.T., Salisbury E., Wang G.-L., Nguyen H., Albrecht J.H., Hershey J.W., Timchenko N.A.
      J. Biol. Chem. 281:32806-32819(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN AN EIF2 COMPLEX WITH EIF2S1; EIF2S2; CALR; CELF1; HSPA5 AND HSP90B1.
    6. "Calsperin is a testis-specific chaperone required for sperm fertility."
      Ikawa M., Tokuhiro K., Yamaguchi R., Benham A.M., Tamura T., Wada I., Satouh Y., Inoue N., Okabe M.
      J. Biol. Chem. 286:5639-5646(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCALR3_MOUSE
    AccessioniPrimary (citable) accession number: Q9D9Q6
    Secondary accession number(s): G5E827
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2003
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3