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Protein

Calreticulin-3

Gene

Calr3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

CALR3 capacity for calcium-binding may be absent or much lower than that of CALR (By similarity). During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109CarbohydrateBy similarity1
Binding sitei111CarbohydrateBy similarity1
Binding sitei128CarbohydrateBy similarity1
Binding sitei135CarbohydrateBy similarity1
Binding sitei300CarbohydrateBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • protein binding involved in protein folding Source: MGI
  • unfolded protein binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDifferentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Calreticulin-3
Alternative name(s):
Calreticulin-2
Calsperin
Gene namesi
Name:Calr3
Synonyms:Crt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1920566 Calr3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Defective sperm migration from the uterus into the oviduct and defective binding to the zona pellucida.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000000417920 – 380Calreticulin-3Add BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi42N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi137 ↔ 163By similarity
Glycosylationi201N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9D9Q6
PeptideAtlasiQ9D9Q6
PRIDEiQ9D9Q6

PTM databases

iPTMnetiQ9D9Q6
PhosphoSitePlusiQ9D9Q6

Expressioni

Tissue specificityi

Testis specific, absent in mature sperm.2 Publications

Gene expression databases

BgeeiENSMUSG00000019732
CleanExiMM_CALR3
ExpressionAtlasiQ9D9Q6 baseline and differential
GenevisibleiQ9D9Q6 MM

Interactioni

Subunit structurei

Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.1 Publication

GO - Molecular functioni

  • protein binding involved in protein folding Source: MGI
  • unfolded protein binding Source: InterPro

Protein-protein interaction databases

DIPiDIP-60025N
IntActiQ9D9Q6, 2 interactors
STRINGi10090.ENSMUSP00000019876

Structurei

3D structure databases

ProteinModelPortaliQ9D9Q6
SMRiQ9D9Q6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati191 – 2021-1Add BLAST12
Repeati209 – 2201-2Add BLAST12
Repeati222 – 2311-310
Repeati235 – 2461-4Add BLAST12
Repeati250 – 2562-17
Repeati260 – 2682-29
Repeati270 – 2802-3Add BLAST11

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 197N-domainAdd BLAST178
Regioni191 – 2464 X approximate repeatsAdd BLAST56
Regioni198 – 291P-domainAdd BLAST94
Regioni250 – 2803 X approximate repeatsAdd BLAST31
Regioni292 – 380C-domainAdd BLAST89

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi377 – 380Prevents secretion from ERSequence analysis4

Sequence similaritiesi

Belongs to the calreticulin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG0674 Eukaryota
ENOG410XRR7 LUCA
GeneTreeiENSGT00430000030841
HOGENOMiHOG000192435
HOVERGENiHBG005407
InParanoidiQ9D9Q6
KOiK10098
OMAiWVQSTND
OrthoDBiEOG091G0AP1
TreeFamiTF338438

Family and domain databases

Gene3Di2.10.250.10, 1 hit
InterProiView protein in InterPro
IPR001580 Calret/calnex
IPR018124 Calret/calnex_CS
IPR009169 Calreticulin
IPR009033 Calreticulin/calnexin_P_dom_sf
IPR013320 ConA-like_dom_sf
PANTHERiPTHR11073 PTHR11073, 1 hit
PfamiView protein in Pfam
PF00262 Calreticulin, 2 hits
PIRSFiPIRSF002356 Calreticulin, 1 hit
PRINTSiPR00626 CALRETICULIN
SUPFAMiSSF49899 SSF49899, 2 hits
SSF63887 SSF63887, 1 hit
PROSITEiView protein in PROSITE
PS00803 CALRETICULIN_1, 1 hit
PS00804 CALRETICULIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9D9Q6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSARALLWA ICVLRVALAT VYFQEEFLDG ERWRNRWVQS TNDSQFGHFR
60 70 80 90 100
VSSGKFYGHK EKDKGLQTTQ NSRFYAISAS FKPFSNKGKT LVIQYTVKHE
110 120 130 140 150
QKMDCGGGYI KVFPSDLDQK KMNGKSQYYI MFGPDICGFD IKKVHVILYF
160 170 180 190 200
KNQYHENKKP IRCKVDGFTH LYTLILRPDL SYEVKVDGQS IESGSIEYDW
210 220 230 240 250
NLTSLRKTEK TSLDSRDWDQ VEGSKVQDWE KHFLDAGASK PSDWNSELDG
260 270 280 290 300
DWLQKPPYED GLKAEGIDKD VWLHQKMRPA GYLTQYDLSE FENIGAIGLE
310 320 330 340 350
LWQVRSGTIF DNFLITDDEE YAEKFGKATW GETKGPEKEM DAIQAKEEVK
360 370 380
KAREEDEEDL LMGKFHRHNH FSRFHRQGEL
Length:380
Mass (Da):44,232
Last modified:October 3, 2012 - v2
Checksum:iB13366ADB11B0442
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti371F → L in BAB24660 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006582 mRNA Translation: BAB24660.1
AC113182 Genomic DNA No translation available.
CH466525 Genomic DNA Translation: EDL10797.1
CCDSiCCDS22413.1
RefSeqiNP_082776.2, NM_028500.3
UniGeneiMm.196315

Genome annotation databases

EnsembliENSMUST00000019876; ENSMUSP00000019876; ENSMUSG00000019732
GeneIDi73316
KEGGimmu:73316
UCSCiuc009mfx.1 mouse

Similar proteinsi

Entry informationi

Entry nameiCALR3_MOUSE
AccessioniPrimary (citable) accession number: Q9D9Q6
Secondary accession number(s): G5E827
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: October 3, 2012
Last modified: March 28, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health