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Q9D9Q6 (CALR3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calreticulin-3
Alternative name(s):
Calreticulin-2
Calsperin
Gene names
Name:Calr3
Synonyms:Crt2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

CALR3 capacity for calcium-binding may be absent or much lower than that of CALR By similarity. During spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility. Ref.6

Subunit structure

Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Ref.5

Subcellular location

Endoplasmic reticulum lumen By similarity.

Tissue specificity

Testis specific, absent in mature sperm. Ref.4 Ref.6

Disruption phenotype

Defective sperm migration from the uterus into the oviduct and defective binding to the zona pellucida. Ref.6

Sequence similarities

Belongs to the calreticulin family.

Ontologies

Keywords
   Biological processDifferentiation
Spermatogenesis
   Cellular componentEndoplasmic reticulum
   DomainRepeat
Signal
   Molecular functionChaperone
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

spermatogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

protein binding involved in protein folding

Inferred from mutant phenotype PubMed 22357757. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 380361Calreticulin-3
PRO_0000004179

Regions

Repeat191 – 202121-1
Repeat209 – 220121-2
Repeat222 – 231101-3
Repeat235 – 246121-4
Repeat250 – 25672-1
Repeat260 – 26892-2
Repeat270 – 280112-3
Region20 – 197178N-domain
Region191 – 246564 X approximate repeats
Region198 – 29194P-domain
Region250 – 280313 X approximate repeats
Region292 – 38089C-domain
Motif377 – 3804Prevents secretion from ER Potential

Sites

Binding site1091Carbohydrate By similarity
Binding site1111Carbohydrate By similarity
Binding site1281Carbohydrate By similarity
Binding site1351Carbohydrate By similarity
Binding site3001Carbohydrate By similarity

Amino acid modifications

Glycosylation421N-linked (GlcNAc...) Potential
Glycosylation2011N-linked (GlcNAc...) Potential
Disulfide bond137 ↔ 163 By similarity

Experimental info

Sequence conflict3711F → L in BAB24660. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9D9Q6 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: B13366ADB11B0442

FASTA38044,232
        10         20         30         40         50         60 
MVSARALLWA ICVLRVALAT VYFQEEFLDG ERWRNRWVQS TNDSQFGHFR VSSGKFYGHK 

        70         80         90        100        110        120 
EKDKGLQTTQ NSRFYAISAS FKPFSNKGKT LVIQYTVKHE QKMDCGGGYI KVFPSDLDQK 

       130        140        150        160        170        180 
KMNGKSQYYI MFGPDICGFD IKKVHVILYF KNQYHENKKP IRCKVDGFTH LYTLILRPDL 

       190        200        210        220        230        240 
SYEVKVDGQS IESGSIEYDW NLTSLRKTEK TSLDSRDWDQ VEGSKVQDWE KHFLDAGASK 

       250        260        270        280        290        300 
PSDWNSELDG DWLQKPPYED GLKAEGIDKD VWLHQKMRPA GYLTQYDLSE FENIGAIGLE 

       310        320        330        340        350        360 
LWQVRSGTIF DNFLITDDEE YAEKFGKATW GETKGPEKEM DAIQAKEEVK KAREEDEEDL 

       370        380 
LMGKFHRHNH FSRFHRQGEL 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Identification of a novel calreticulin isoform (Crt2) in human and mouse."
Persson S., Rosenquist M., Sommarin M.
Gene 297:151-158(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[5]"Age-specific CUGBP1-eIF2 complex increases translation of CCAAT/enhancer-binding protein beta in old liver."
Timchenko L.T., Salisbury E., Wang G.-L., Nguyen H., Albrecht J.H., Hershey J.W., Timchenko N.A.
J. Biol. Chem. 281:32806-32819(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN AN EIF2 COMPLEX WITH EIF2S1; EIF2S2; CALR; CELF1; HSPA5 AND HSP90B1.
[6]"Calsperin is a testis-specific chaperone required for sperm fertility."
Ikawa M., Tokuhiro K., Yamaguchi R., Benham A.M., Tamura T., Wada I., Satouh Y., Inoue N., Okabe M.
J. Biol. Chem. 286:5639-5646(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK006582 mRNA. Translation: BAB24660.1.
AC113182 Genomic DNA. No translation available.
CH466525 Genomic DNA. Translation: EDL10797.1.
RefSeqNP_082776.2. NM_028500.3.
UniGeneMm.196315.

3D structure databases

ProteinModelPortalQ9D9Q6.
SMRQ9D9Q6. Positions 21-348.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60025N.

PTM databases

PhosphoSiteQ9D9Q6.

Proteomic databases

PaxDbQ9D9Q6.
PRIDEQ9D9Q6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019876; ENSMUSP00000019876; ENSMUSG00000019732.
GeneID73316.
KEGGmmu:73316.
UCSCuc009mfx.1. mouse.

Organism-specific databases

CTD125972.
MGIMGI:1920566. Calr3.

Phylogenomic databases

eggNOGNOG268919.
GeneTreeENSGT00430000030841.
HOGENOMHOG000192435.
HOVERGENHBG005407.
InParanoidQ9D9Q6.
KOK10098.
OMAQDWEKHF.
OrthoDBEOG77126Z.
TreeFamTF338438.

Gene expression databases

CleanExMM_CALR3.
GenevestigatorQ9D9Q6.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR001580. Calret/calnex.
IPR018124. Calret/calnex_CS.
IPR009169. Calreticulin.
IPR009033. Calreticulin/calnexin_P_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
[Graphical view]
PANTHERPTHR11073. PTHR11073. 1 hit.
PfamPF00262. Calreticulin. 1 hit.
[Graphical view]
PIRSFPIRSF002356. Calreticulin. 1 hit.
PRINTSPR00626. CALRETICULIN.
SUPFAMSSF49899. SSF49899. 2 hits.
SSF63887. SSF63887. 1 hit.
PROSITEPS00803. CALRETICULIN_1. 1 hit.
PS00804. CALRETICULIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio337937.
PROQ9D9Q6.
SOURCESearch...

Entry information

Entry nameCALR3_MOUSE
AccessionPrimary (citable) accession number: Q9D9Q6
Secondary accession number(s): G5E827
Entry history
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: October 3, 2012
Last modified: April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot