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Protein

Ubiquitin carboxyl-terminal hydrolase 12

Gene

Usp12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Activated by interaction with WDR20 and WDR48 through different allosteric mechanisms.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48NucleophilePROSITE-ProRule annotation1
Metal bindingi186ZincBy similarity1
Metal bindingi189ZincBy similarity1
Metal bindingi233ZincBy similarity1
Metal bindingi236ZincBy similarity1
Active sitei317Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.020

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 12
Ubiquitin thioesterase 12
Ubiquitin-hydrolyzing enzyme 1
Ubiquitin-specific-processing protease 12
Gene namesi
Name:Usp12
Synonyms:Ubh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1270128 Usp12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002603121 – 370Ubiquitin carboxyl-terminal hydrolase 12Add BLAST370

Proteomic databases

PaxDbiQ9D9M2
PeptideAtlasiQ9D9M2
PRIDEiQ9D9M2

PTM databases

iPTMnetiQ9D9M2
PhosphoSitePlusiQ9D9M2
SwissPalmiQ9D9M2

Expressioni

Gene expression databases

BgeeiENSMUSG00000029640
CleanExiMM_USP12
GenevisibleiQ9D9M2 MM

Interactioni

Subunit structurei

Interacts with WDR48. Interacts with WDR20. Component of the USP12/WDR20/WDR48 deubiquitinating complex.By similarity

Protein-protein interaction databases

BioGridi204419, 1 interactor
IntActiQ9D9M2, 2 interactors
MINTiQ9D9M2
STRINGi10090.ENSMUSP00000082754

Structurei

3D structure databases

ProteinModelPortaliQ9D9M2
SMRiQ9D9M2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 369USPAdd BLAST331

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1864 Eukaryota
ENOG410XQ81 LUCA
GeneTreeiENSGT00890000139367
HOGENOMiHOG000231498
HOVERGENiHBG054038
InParanoidiQ9D9M2
KOiK11842
OMAiTANCKIN
OrthoDBiEOG091G0FC5
PhylomeDBiQ9D9M2
TreeFamiTF314144

Family and domain databases

InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9D9M2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEILMTVSKF ASICTMGANA SALEKEIGPE QFPVNEHYFG LVNFGNTCYC
60 70 80 90 100
NSVLQALYFC RPFREKVLAY KSQPRKKENL LTCLADLFHS IATQKKKVGV
110 120 130 140 150
IPPKKFITRL RKENELFDNY MQQDAHEFLN YLLNTIADIL QEERKQEKQN
160 170 180 190 200
GRLRNGDVDN EDNNSTPDPT WVHEIFQGTL TNETRCLTCE TISSKDEDFL
210 220 230 240 250
DLSVDVEQNT SITHCLRGFS NTETLCSEYK YYCEECRSKQ EAHKRMKVKK
260 270 280 290 300
LPLILALHLK RFKYMDQLHR YTKLSYRVVF PLELRLFNTS GDATNPDRMY
310 320 330 340 350
DLVAVVVHCG SGPNRGHYIA IVKSHDFWLL FDDDIVEKID AQAIEEFYGL
360 370
TSDISKNSES GYILFYQSRD
Length:370
Mass (Da):42,914
Last modified:July 5, 2004 - v2
Checksum:iB11AC16D9B12EBB8
GO
Isoform 2 (identifier: Q9D9M2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-120: Missing.

Note: No experimental confirmation available.
Show »
Length:250
Mass (Da):29,157
Checksum:i4FBC07CA0DABD050
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0376171 – 120Missing in isoform 2. 1 PublicationAdd BLAST120

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006739 mRNA Translation: BAB24720.2
AK035629 mRNA Translation: BAC29129.1
AK167153 mRNA Translation: BAE39295.1
BC049274 mRNA Translation: AAH49274.1
BC055398 mRNA Translation: AAH55398.1
BC068136 mRNA Translation: AAH68136.1
AF441835 mRNA Translation: AAL86740.1
CCDSiCCDS39395.1 [Q9D9M2-1]
RefSeqiNP_035799.1, NM_011669.3 [Q9D9M2-1]
XP_006504881.1, XM_006504818.2 [Q9D9M2-2]
UniGeneiMm.167971

Genome annotation databases

EnsembliENSMUST00000085614; ENSMUSP00000082754; ENSMUSG00000029640 [Q9D9M2-1]
GeneIDi22217
KEGGimmu:22217
UCSCiuc009anj.1 mouse [Q9D9M2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBP12_MOUSE
AccessioniPrimary (citable) accession number: Q9D9M2
Secondary accession number(s): Q790B0
, Q7TNV4, Q80Y43, Q8CBN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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