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Q9D9M2

- UBP12_MOUSE

UniProt

Q9D9M2 - UBP12_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase 12

Gene

Usp12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2 By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei48 – 481NucleophilePROSITE-ProRule annotation
    Active sitei317 – 3171Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. protein deubiquitination Source: UniProtKB
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.020.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 12 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 12
    Ubiquitin thioesterase 12
    Ubiquitin-hydrolyzing enzyme 1
    Ubiquitin-specific-processing protease 12
    Gene namesi
    Name:Usp12
    Synonyms:Ubh1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1270128. Usp12.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Ubiquitin carboxyl-terminal hydrolase 12PRO_0000260312Add
    BLAST

    Proteomic databases

    PaxDbiQ9D9M2.
    PRIDEiQ9D9M2.

    PTM databases

    PhosphoSiteiQ9D9M2.

    Expressioni

    Gene expression databases

    BgeeiQ9D9M2.
    CleanExiMM_USP12.
    GenevestigatoriQ9D9M2.

    Interactioni

    Subunit structurei

    Interacts with WDR48.By similarity

    Protein-protein interaction databases

    IntActiQ9D9M2. 1 interaction.
    MINTiMINT-4127824.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9D9M2.
    SMRiQ9D9M2. Positions 40-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 369331USPAdd
    BLAST

    Sequence similaritiesi

    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    GeneTreeiENSGT00650000093027.
    HOGENOMiHOG000231498.
    HOVERGENiHBG054038.
    InParanoidiQ80Y43.
    KOiK11842.
    OMAiSKFASFC.
    OrthoDBiEOG7RBZ8G.
    PhylomeDBiQ9D9M2.
    TreeFamiTF314144.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9D9M2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEILMTVSKF ASICTMGANA SALEKEIGPE QFPVNEHYFG LVNFGNTCYC    50
    NSVLQALYFC RPFREKVLAY KSQPRKKENL LTCLADLFHS IATQKKKVGV 100
    IPPKKFITRL RKENELFDNY MQQDAHEFLN YLLNTIADIL QEERKQEKQN 150
    GRLRNGDVDN EDNNSTPDPT WVHEIFQGTL TNETRCLTCE TISSKDEDFL 200
    DLSVDVEQNT SITHCLRGFS NTETLCSEYK YYCEECRSKQ EAHKRMKVKK 250
    LPLILALHLK RFKYMDQLHR YTKLSYRVVF PLELRLFNTS GDATNPDRMY 300
    DLVAVVVHCG SGPNRGHYIA IVKSHDFWLL FDDDIVEKID AQAIEEFYGL 350
    TSDISKNSES GYILFYQSRD 370
    Length:370
    Mass (Da):42,914
    Last modified:July 5, 2004 - v2
    Checksum:iB11AC16D9B12EBB8
    GO
    Isoform 2 (identifier: Q9D9M2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-120: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:250
    Mass (Da):29,157
    Checksum:i4FBC07CA0DABD050
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 120120Missing in isoform 2. 1 PublicationVSP_037617Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK006739 mRNA. Translation: BAB24720.2.
    AK035629 mRNA. Translation: BAC29129.1.
    AK167153 mRNA. Translation: BAE39295.1.
    BC049274 mRNA. Translation: AAH49274.1.
    BC055398 mRNA. Translation: AAH55398.1.
    BC068136 mRNA. Translation: AAH68136.1.
    AF441835 mRNA. Translation: AAL86740.1.
    CCDSiCCDS39395.1. [Q9D9M2-1]
    RefSeqiNP_035799.1. NM_011669.3. [Q9D9M2-1]
    XP_006504880.1. XM_006504817.1.
    XP_006504881.1. XM_006504818.1. [Q9D9M2-2]
    UniGeneiMm.167971.

    Genome annotation databases

    EnsembliENSMUST00000085614; ENSMUSP00000082754; ENSMUSG00000029640. [Q9D9M2-1]
    GeneIDi22217.
    KEGGimmu:22217.
    UCSCiuc009anj.1. mouse. [Q9D9M2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK006739 mRNA. Translation: BAB24720.2 .
    AK035629 mRNA. Translation: BAC29129.1 .
    AK167153 mRNA. Translation: BAE39295.1 .
    BC049274 mRNA. Translation: AAH49274.1 .
    BC055398 mRNA. Translation: AAH55398.1 .
    BC068136 mRNA. Translation: AAH68136.1 .
    AF441835 mRNA. Translation: AAL86740.1 .
    CCDSi CCDS39395.1. [Q9D9M2-1 ]
    RefSeqi NP_035799.1. NM_011669.3. [Q9D9M2-1 ]
    XP_006504880.1. XM_006504817.1.
    XP_006504881.1. XM_006504818.1. [Q9D9M2-2 ]
    UniGenei Mm.167971.

    3D structure databases

    ProteinModelPortali Q9D9M2.
    SMRi Q9D9M2. Positions 40-369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9D9M2. 1 interaction.
    MINTi MINT-4127824.

    Protein family/group databases

    MEROPSi C19.020.

    PTM databases

    PhosphoSitei Q9D9M2.

    Proteomic databases

    PaxDbi Q9D9M2.
    PRIDEi Q9D9M2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000085614 ; ENSMUSP00000082754 ; ENSMUSG00000029640 . [Q9D9M2-1 ]
    GeneIDi 22217.
    KEGGi mmu:22217.
    UCSCi uc009anj.1. mouse. [Q9D9M2-1 ]

    Organism-specific databases

    CTDi 219333.
    MGIi MGI:1270128. Usp12.

    Phylogenomic databases

    eggNOGi COG5077.
    GeneTreei ENSGT00650000093027.
    HOGENOMi HOG000231498.
    HOVERGENi HBG054038.
    InParanoidi Q80Y43.
    KOi K11842.
    OMAi SKFASFC.
    OrthoDBi EOG7RBZ8G.
    PhylomeDBi Q9D9M2.
    TreeFami TF314144.

    Miscellaneous databases

    NextBioi 302225.
    PROi Q9D9M2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9D9M2.
    CleanExi MM_USP12.
    Genevestigatori Q9D9M2.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Testis and Urinary bladder.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6 and FVB/N.
      Tissue: Brain and Liver.
    3. "Molecular cloning and complete cDNA sequence of UBH1 in mouse testis."
      Baek K.-H., Park K.-H., Kim Y.-S., Kim M.-S., Choi H.-K.
      DNA Seq. 13:145-148(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-370 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Testis.

    Entry informationi

    Entry nameiUBP12_MOUSE
    AccessioniPrimary (citable) accession number: Q9D9M2
    Secondary accession number(s): Q790B0
    , Q7TNV4, Q80Y43, Q8CBN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3